Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGW_NcoI-Eco109I
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00744903
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4008nts
Open reading frame: 1 to 4008
ATGAGGGGCGCAGGAGGACCTAGAGGGCCTAGAGGACCTGCTAAGATGCTTCTGCTGCTT
GCCCTCGCTTGTGCCTCTCCTTTTCCTGAAGAAGCCCCAGGGCCCGGAGGCGCCGGAGGA
CCCGGAGGAGGACTTGGCGGAGCCAGACCCCTTAATGTTGCCCTGGTGTTTTCTGGGCCT
GCCTATGCTGCCGAGGCCGCTAGACTTGGACCTGCCGTGGCCGCCGCTGTGCGATCTCCT
GGCTTGGATGTGAGGCCAGTGGCCCTGGTTCTGAATGGATCTGATCCTAGATCTCTCGTG
CTCCAACTGTGTGATCTGCTGTCCGGACTCAGGGTGCACGGAGTGGTTTTTGAAGATGAT
TCTAGAGCTCCCGCCGTGGCTCCTATTCTGGATTTTTTATCAGCCCAGACATCTTTACCT
ATTGTGGCCGTGCATGGAGGAGCCGCCCTTGTGCTCACACCTAAAGAAAAAGGATCTACA
TTTCTGCAGCTGGGGTCTTCTACTGAGCAACAGTTGCAAGTGATATTTGAAGTGCTGGAA
GAATATGATTGGACAAGTTTTGTAGCCGTGACAACAAGGGCCCCTGGACATAGAGCCTTT
TTGTCTTATATTGAAGTTCTGACAGATGGCTCTCTGGTTGGATGGGAACATAGAGGAGCA
CTCACACTGGATCCTGGGGCCGGAGAGGCCGTGCTGTCTGCTCAGCTTAGATCTGTGTCT
GCCCAAATTAGACTGCTGTTTTGTGCTAGAGAAGAGGCCGAACCAGTCTTTAGAGCCGCC
GAAGAGGCTGGACTCACAGGATCAGGCTATGTGTGGTTTATGGTCGGACCTCAGCTCGCT
GGAGGCGGCGGATCCGGAGCTCCAGGAGAACCTCCTCTGCTTCCAGGCGGGGCCCCACTT
CCTGCCGGACTGTTTGCCGTGAGATCCGCTGGATGGCGGGATGATCTGGCTAGGAGAGTG
GCTGCCGGAGTGGCCGTTGTGGCCAGAGGAGCCCAGGCTCTGCTGAGAGATTATGGGTTT
CTGCCTGAACTGGGACATGATTGTAGAGCACAGAATAGAACTCATAGAGGCGAGTCTCTG
CATCGTTATTTTATGAATATTACCTGGGATAATAGGGATTATTCTTTTAATGAAGATGGC
TTTCTGGTGAATCCATCTCTGGTGGTTATTTCTCTCACAAGAGATAGGACATGGGAAGTG
GTTGGGAGCTGGGAGCAACAAACACTGAGATTAAAATATCCTCTTTGGTCAAGATATGGC
CGCTTTCTTCAGCCTGTGGATGATACACAGCATCTGACAGTGGCCACACTCGAAGAAAGA
CCGTTTGTGATTGTCGAACCTGCCGATCCTATTAGCGGAACATGTATTAGAGATAGCGTG
CCTTGTAGATCTCAGCTCAACAGAACTCACTCTCCACCACCTGATGCCCCCAGACCTGAA
AAAAGATGTTGTAAAGGATTTTGTATTGATATTCTGAAAAGGCTGGCTCATACAATCGGA
TTTAGCTATGATCTGTATCTGGTTACAAATGGAAAACATGGAAAAAAAATTGATGGAGTG
TGGAATGGAATGATTGGCGAAGTGTTTTACCAGCGGGCCGACATGGCCATTGGATCTCTT
ACAATTAATGAGGAAAGATCTGAGATTGTGGATTTCTCTGTGCCATTTGTGGAAACAGGA
ATTTCTGTGATGGTGGCCAGGTCTAATGGTACAGTGTCTCCTTCTGCCTTTCTGGAACCT
TATAGCCCTGCTGTGTGGGTGATGATGTTTGTTATGTGTCTCACAGTGGTTGCCGTTACA
GTGTTTATTTTTGAATATCTGTCTCCAGTGGGATATAATAGGTCACTGGCAACAGGAAAG
AGGCCTGGAGGGTCTACATTCACAATTGGCAAATCTATTTGGCTGCTGTGGGCACTGGTT
TTTAATAATAGCGTTCCTGTGGAGAATCCTAGGGGAACAACAAGCAAAATTATGGTTCTG
GTGTGGGCTTTTTTTGCAGTGATTTTTCTGGCAAGTTATACAGCCAATCTTGCCGCTTTT
ATGATTCAAGAGGAATATGTGGATACAGTTTCTGGCCTTTCAGATAGAAAATTTCAGAGG
CCTCAGGAACAGTATCCTCCTCTCAAATTTGGAACAGTGCCTAATGGCTCTACAGAAAAA
AATATTAGAAGCAATTATCCTGATATGCATTCTTATATGGTGAGATATAATCAGCCTAGA
GTGGAAGAAGCTCTGACACAGCTGAAAGCCGGAAAACTGGACGCCTTTATTTATGATGCC
GCCGTGCTTAATTATATGGCTAGAAAGGATGAAGGCTGTAAACTGGTGACTATCGGATCT
GGAAAAGTGTTCGCTACAACAGGATATGGCATTGCCCTGCATAAAGGGTCTAGATGGAAA
AGACCTATTGACCTCGCTCTCCTGCAGTTTCTCGGAGATGATGAAATTGAAATGCTCGAG
AGGCTTTGGCTGTCTGGAATTTGTCACAATGATAAAATTGAGGTGATGTCTAGCAAACTG
GATATTGATAATATGGCAGGAGTTTTTTATATGCTGCTCGTGGCTATGGGACTGTCTCTC
CTGGTATTTGCCTGGGAACACCTGGTGTATTGGAGACTCAGACATTGTCTGGGGCCTACA
CATAGAATGGATTTTCTGCTCGCTTTTTCTAGAGGAATGTATTCTTGTTGTAGTGCCGAA
GCCGCCCCACCTCCCGCTAAACCCCCTCCTCCACCTCAACCTCTTCCTTCTCCTGCTTAT
CCTGCCCCTAGACCTGCTCCTGGCCCCGCTCCTTTTGTGCCAAGAGAAAGAGCCTCTGTG
GATAGATGGAGAAGAACAAAAGGCGCTGGACCTCCAGGGGGAGCGGGACTGGCCGATGGA
TTTCATAGATATTATGGACCTATCGAACCTCAGGGCCTGGGTCTGGGACTGGGCGAAGCC
AGAGCTGCTCCTCGGGGAGCTGCAGGAAGACCACTCAGCCCACCCGCTGCCCAGCCTCCA
CAGAAGCCACCTCCTTCTTATTTTGCCATTGTGAGGGATAAAGAACCTGCTGAACCACCC
GCCGGAGCCTTTCCAGGATTCCCTAGCCCTCCCGCCCCTCCAGCTGCCGCCGCCACAGCC
GTTGGACCACCTCTCTGTAGACTGGCCTTCGAAGATGAGAGCCCACCAGCTCCGGCCAGA
TGGCCAAGAAGCGATCCTGAATCTCAGCCCCTTTTGGGACCTGGCGCTGGTGGTGCTGGA
GGAACAGGAGGGGCTGGCGGAGGAGCTCCTGCTGCTCCCCCACCTTGTAGGGCTGCACCT
CCTCCTTGTCCTTATCTGGATCTCGAACCAAGCCCTTCTGATTCTGAAGATAGCGAAAGT
CTGGGCGGCGCTAGCCTGGGAGGCCTTGAACCTTGGTGGTTTGCCGATTTTCCTTATCCA
TATGCCGAGAGACTGGGACCACCGCCAGGAAGGTATTGGTCAGTGGATAAACTGGGTGGC
TGGAGAGCCGGAAGCTGGGATTATCTCCCTCCACGGAGCGGACCTGCGGCCTGGCATTGT
AGGCATTGTGCTTCTCTGGAACTTCTGCCACCTCCAAGGCATCTGTCTTGTTCTCATGAT
GGACTGGATGGAGGATGGTGGGCACCTCCCCCCCCTCCCTGGGCCGCCGGACCTCTCCCA
AGAAGAAGAGCCAGATGTGGATGTCCAAGATCCCATCCTCATAGACCTAGGGCTTCTCAC
AGAACACCAGCTGCTGCTGCCCCCCATCATCATAGACATAGGAGGGCCGCTGGCGGCTGG
GATCTGCCTCCACCCGCACCTACTTCTAGGAGTCTGGAAGATCTGTCTAGCTGTCCAAGG
GCCGCCCCTGCAAGAAGACTGACAGGCCCTTCTCGCCATGCTAGAAGATGTCCTCATGCC
GCCCATTGGGGCCCACCTCTGCCAACAGCCTCTCATAGAAGACACAGGGGAGGCGATCTG
GGCACAAGAAGAGGAAGCGCCCATTTTTCTTCTCTCGAATCTGAAGTG
Protein sequence predicted based on nucleotide sequence
MRGAGGPRGPRGPAKMLLLLALACASPFPEEAPGPGGAGGPGGGLGGARPLNVALVFSGP AYAAEAARLGPAVAAAVRSPGLDVRPVALVLNGSDPRSLVLQLCDLLSGLRVHGVVFEDD SRAPAVAPILDFLSAQTSLPIVAVHGGAALVLTPKEKGSTFLQLGSSTEQQLQVIFEVLE EYDWTSFVAVTTRAPGHRAFLSYIEVLTDGSLVGWEHRGALTLDPGAGEAVLSAQLRSVS AQIRLLFCAREEAEPVFRAAEEAGLTGSGYVWFMVGPQLAGGGGSGAPGEPPLLPGGAPL PAGLFAVRSAGWRDDLARRVAAGVAVVARGAQALLRDYGFLPELGHDCRAQNRTHRGESL HRYFMNITWDNRDYSFNEDGFLVNPSLVVISLTRDRTWEVVGSWEQQTLRLKYPLWSRYG RFLQPVDDTQHLTVATLEERPFVIVEPADPISGTCIRDSVPCRSQLNRTHSPPPDAPRPE KRCCKGFCIDILKRLAHTIGFSYDLYLVTNGKHGKKIDGVWNGMIGEVFYQRADMAIGSL TINEERSEIVDFSVPFVETGISVMVARSNGTVSPSAFLEPYSPAVWVMMFVMCLTVVAVT VFIFEYLSPVGYNRSLATGKRPGGSTFTIGKSIWLLWALVFNNSVPVENPRGTTSKIMVL VWAFFAVIFLASYTANLAAFMIQEEYVDTVSGLSDRKFQRPQEQYPPLKFGTVPNGSTEK NIRSNYPDMHSYMVRYNQPRVEEALTQLKAGKLDAFIYDAAVLNYMARKDEGCKLVTIGS GKVFATTGYGIALHKGSRWKRPIDLALLQFLGDDEIEMLERLWLSGICHNDKIEVMSSKL DIDNMAGVFYMLLVAMGLSLLVFAWEHLVYWRLRHCLGPTHRMDFLLAFSRGMYSCCSAE AAPPPAKPPPPPQPLPSPAYPAPRPAPGPAPFVPRERASVDRWRRTKGAGPPGGAGLADG FHRYYGPIEPQGLGLGLGEARAAPRGAAGRPLSPPAAQPPQKPPPSYFAIVRDKEPAEPP AGAFPGFPSPPAPPAAAATAVGPPLCRLAFEDESPPAPARWPRSDPESQPLLGPGAGGAG GTGGAGGGAPAAPPPCRAAPPPCPYLDLEPSPSDSEDSESLGGASLGGLEPWWFADFPYP YAERLGPPPGRYWSVDKLGGWRAGSWDYLPPRSGPAAWHCRHCASLELLPPPRHLSCSHD GLDGGWWAPPPPPWAAGPLPRRRARCGCPRSHPHRPRASHRTPAAAAPHHHRHRRAAGGW DLPPPAPTSRSLEDLSSCPRAAPARRLTGPSRHARRCPHAAHWGPPLPTASHRRHRGGDL GTRRGSAHFSSLESEV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGW_NcoI-Eco109I
Synonyms:
pGW-NcoEco
Description:
In-fusion cloning vector with NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette; kanamycin resistance in bacteria; recombinational cloning
Comments:
NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette. Made for use with In-Fusion cloning
Size (bp):
4008
Parent Vector:
None
Properties:
''
Author Name:
Joshua LaBaer
Andreas Rolf
Andreas Rolf
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3332
4005
death cassette
ccdB
ccdB death cassette - removed in plasmids containing an insert
1779
2084
primer
M13 forward
M13 forward sequencing primer
536
553
primer
M13 reverse
M13 reverse sequencing primer
2269
2289
recombination site
attL1
attL1 recombinational cloning site
644
668
recombination site
attL2
attL2 recombinational cloning site
2133
2157
selectable marker
KanR
kanamycin resistance
2402
3208
selectable marker
CmR
Chloramphenicol resistance - removed in plasmids containing an insert
778
1434
