Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745100
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5373nts
Open reading frame: 1 to 5373
ATGATGTGCTTAAAGATCCTAAGAATAAGCCTGGCGATTTTGGCTGGGTGGGCACTCTGT
TCTGCCAACTCTGAGCTGGGCTGGACACGCAAGAAATCCTTGGTTGAGAGGGAACACCTG
AATCAGGTGCTGTTGGAAGGAGAACGTTGTTGGCTGGGGGCCAAGGTTCGAAGACCCAGA
GCTTCTCCACAGCATCACCTCTTTGGAGTCTACCCCAGCAGGGCTGGGAACTACCTAAGG
CCCTACCCCGTGGGGGAGCAAGAAATCCATCATACAGGACGCAGCAAACCAGACACTGAA
GGAAATGCTGTGAGCCTTGTTCCCCCAGACCTGACTGAAAATCCAGCAGGACTGAGGGGT
GCAGTTGAAGAGCCGGCTGCCCCATGGGTAGGGGATAGTCCTATTGGGCAATCTGAGCTG
CTGGGAGATGATGACGCTTATCTCGGCAATCAAAGATCCAAGGAGTCTCTAGGTGAGGCC
GGGATTCAGAAAGGCTCAGCCATGGCTGCCACTACTACCACCGCCATTTTCACAACCCTG
AACGAACCCAAACCAGAGACGCAAAGGAGGGGCTGGGCCAAGTCCAGGCAGCGTCGCCAA
GTGTGGAAGAGGCGGGCGGAAGATGGGCAGGGAGACTCCGGTATCTCTTCACATTTCCAA
CCTTGGCCCAAGCATTCCCTTAAACACAGGGTCAAAAAGAGTCCACCGGAGGAAAGCAAC
CAAAATGGTGGAGAGGGCTCCTACCGAGAAGCAGAGACATTTAACTCCCAAGTAGGACTG
CCCATCTTATACTTCTCTGGGAGGCGGGAGCGGCTGCTGCTGCGTCCAGAAGTGCTGGCT
GAGATTCCCCGGGAGGCGTTCACAGTGGAAGCCTGGGTTAAACCGGAGGGAGGACAGAAC
AACCCAGCCATCATCGCAGGGGTGTTTGATAACTGCTCCCACACTGTCAGTGACAAAGGC
TGGGCCCTGGGGATCCGCTCAGGGAAGGACAAGGGAAAGCGGGATGCTCGCTTCTTCTTC
TCCCTCTGCACCGACCGCGTGAAGAAAGCCACCATCTTGATTAGCCACAGTCGCTACCAA
CCAGGCACATGGACCCATGTGGCAGCCACTTACGATGGACGGCACATGGCCCTGTATGTG
GATGGCACTCAGGTGGCTAGCAGTCTAGACCAGTCTGGTCCCCTGAACAGCCCCTTCATG
GCATCTTGCCGCTCTTTGCTCCTAGGGGGAGACAGCTCTGAGGATGGGCACTATTTCCGT
GGACACCTGGGCACACTGGTTTTCTGGTCGACCGCCCTGCCACAAAGCCATTTTCAGCAC
AGTTCTCAGCATTCAAGTGGGGAGGAGGAAGCGACTGACTTGGTCCTGACAGCGAGCTTT
GAGCCTGTGAACACAGAGTGGGTTCCCTTTAGAGATGAGAAGTACCCACGACTTGAGGTT
CTCCAGGGCTTTGAGCCAGAGCCTGAGATTCTGTCGCCTTTGCAGCCCCCACTCTGTGGG
CAAACAGTCTGTGACAATGTGGAATTGATCTCCCAGTACAATGGATACTGGCCCCTTCGG
GGAGAGAAGGTGATACGCTACCAGGTGGTGAACATCTGTGATGATGAGGGCCTAAACCCC
ATTGTGAGTGAGGAGCAGATTCGTCTGCAGCACGAGGCACTGAATGAGGCCTTCAGCCGC
TACAACATCAGCTGGCAGCTGAGCGTCCACCAGGTCCACAATTCCACCCTGCGACACCGG
GTTGTGCTTGTGAACTGTGAGCCCAGCAAGATTGGCAATGACCATTGTGACCCCGAGTGT
GAGCACCCACTCACAGGCTATGATGGGGGTGACTGCCGCCTGCAGGGCCGCTGCTACTCC
TGGAACCGCAGGGATGGGCTCTGTCACGTGGAGTGTAACAACATGCTGAACGACTTTGAC
GACGGAGACTGCTGCGACCCCCAGGTGGCTGATGTGCGCAAGACCTGCTTTGACCCTGAC
TCACCCAAGAGGGCATACATGAGTGTGAAGGAGCTGAAGGAGGCCCTGCAGCTGAACAGT
ACTCACTTCCTCAACATCTACTTTGCCAGCTCAGTGCGGGAAGACCTTGCTGGTGCTGCC
ACCTGGCCTTGGGACAAGGACGCTGTCACTCACCTGGGTGGCATTGTCCTCAGCCCAGCA
TATTATGGGATGCCTGGCCACACCGACACCATGATCCATGAAGTGGGACATGTTCTGGGA
CTCTACCATGTCTTTAAAGGAGTCAGTGAAAGAGAATCCTGCAATGACCCCTGCAAGGAG
ACAGTGCCATCCATGGAAACGGGAGACTTATGTGCCGACACCGCCCCCACTCCCAAGAGT
GAGCTGTGCCGGGAACCAGAGCCCACTAGTGACACCTGTGGCTTCACTCGCTTCCCAGGG
GCTCCGTTCACCAACTACATGAGCTACACGGATGATAACTGCACTGACAACTTCACTCCT
AACCAAGTGGCCCGAATGCATTGCTATTTGGACCTAGTCTATCAGCAGTGGACTGAAAGC
AGAAAGCCCACCCCCATCCCCATTCCACCTATGGTCATCGGACAGACCAACAAGTCCCTC
ACTATCCACTGGCTGCCTCCTATTAGTGGAGTTGTATATGACAGGGCCTCAGGCAGCTTG
TGTGGCGCTTGCACTGAAGATGGGACCTTTCGTCAGTATGTGCACACAGCTTCCTCCCGG
CGGGTGTGTGACTCCTCAGGTTATTGGACCCCAGAGGAGGCTGTGGGGCCTCCTGATGTG
GATCAGCCCTGCGAGCCAAGCTTACAGGCCTGGAGCCCTGAGGTCCACCTGTACCACATG
AACATGACGGTCCCCTGCCCCACAGAAGGCTGTAGCTTGGAGCTGCTCTTCCAACACCCG
GTCCAAGCCGACACCCTCACCCTGTGGGTCACTTCCTTCTTCATGGAGTCCTCGCAGGTC
CTCTTTGACACAGAGATCTTGCTGGAAAACAAGGAGTCAGTGCACCTGGGCCCCTTAGAC
ACTTTCTGTGACATCCCACTCACCATCAAACTGCACGTGGATGGGAAGGTGTCGGGGGTG
AAAGTCTACACCTTTGATGAGAGGATAGAGATTGATGCAGCACTCCTGACTTCTCAGCCC
CACAGTCCCTTGTGCTCTGGCTGCAGGCCTGTGAGGTACCAGGTTCTCCGCGATCCCCCA
TTTGCCAGTGGTTTGCCCGTGGTGGTGACACATTCTCACAGGAAGTTCACGGACGTGGAG
GTCACACCTGGACAGATGTATCAGTACCAAGTTCTAGCTGAAGCTGGAGGAGAACTGGGA
GAAGCTTCGCCTCCTCTGAACCACATTCATGGAGCTCCTTATTGTGGAGATGGGAAGGTG
TCAGAGAGACTGGGAGAAGAGTGTGATGATGGGGACCTTGTGAGCGGAGATGGCTGCTCC
AAGGTGTGTGAGCTGGAGGAAGGTTTCAACTGTGTAGGAGAGCCAAGCCTTTGCTACATG
TATGAGGGAGATGGCATATGTGAACCTTTTGAGAGAAAAACCAGCATTGTAGACTGTGGC
ATCTACACTCCCAAAGGATACTTGGATCAATGGGCTACCCGGGCTTACTCCTCTCATGAA
GACAAGAAGAAGTGTCCTGTTTCCTTGGTAACTGGAGAACCTCATTCCCTAATTTGCACA
TCATACCATCCAGATTTACCCAACCACCGTCCCCTAACTGGCTGGTTTCCCTGTGTTGCC
AGTGAAAATGAAACTCAGGATGACAGGAGTGAACAGCCAGAAGGTAGCCTGAAGAAAGAG
GATGAGGTTTGGCTCAAAGTGTGTTTCAATAGACCAGGAGAGGCCAGAGCAATTTTTATT
TTTTTGACAACTGATGGCCTAGTTCCCGGAGAGCATCAGCAGCCGACAGTGACTCTCTAC
CTGACCGATGTCCGTGGAAGCAACCACTCTCTTGGAACCTATGGACTGTCATGCCAGCAT
AATCCACTGATTATCAATGTGACCCATCACCAGAATGTCCTTTTCCACCATACCACCTCA
GTGCTGCTGAATTTCTCATCCCCACGGGTCGGCATCTCAGCTGTGGCTCTAAGGACATCC
TCCCGCATTGGTCTTTCGGCTCCCAGTAACTGCATCTCAGAGGACGAGGGGCAGAATCAT
CAGGGACAGAGCTGTATCCATCGGCCCTGTGGGAAGCAGGACAGCTGTCCGTCATTGCTG
CTTGATCATGCTGATGTGGTGAACTGTACCTCTATAGGCCCAGGACTCATGAAGTGTGCT
ATCACTTGTCAAAGGGGATTTGCCCTTCAGGCCAGCAGTGGGCAGTACATCAGGCCCATG
CAGAAGGAAATTCTGCTCACATGTTCTTCTGGGCACTGGGACCAGAATGTGAGCTGCCTT
CCCGTGGACTGCGGTGTTCCCGACCCGTCTTTGGTGAACTATGCAAACTTCTCCTGCTCA
GAGGGAACCAAATTTCTGAAACGCTGCTCAATCTCTTGTGTCCCACCAGCCAAGCTGCAA
GGACTGAGCCCATGGCTGACATGTCTTGAAGATGGGCTCTGGTCGCTCCCTGAAGTCTAC
TGCAAGTTGGAGTGTGATGCTCCCCCTATTATTCTGAATGCCAACTTGCTCCTGCCTCAC
TGCCTCCAGGACAACCACGACGTGGGCACCATCTGCAAATATGAATGCAAACCAGGGTAC
TATGTGGCAGAAAGTGCAGAGGGTAAAGTCAGGAACAAGCTCCTGAAGATACAATGCCTG
GAAGGTGGAATCTGGGAGCAAGGCAGCTGCATTCCTGTGGTGTGTGAGCCACCCCCTCCT
GTGTTTGAAGGCATGTATGAATGTACCAATGGCTTCAGCCTGGACAGCCAGTGTGTGCTC
AACTGTAACCAGGAACGTGAAAAGCTTCCCATCCTCTGCACTAAAGAGGGCCTGTGGACC
CAGGAGTTTAAGTTGTGTGAGAATCTGCAAGGAGAATGCCCACCACCCCCCTCAGAGCTG
AATTCTGTGGAGTACAAATGTGAACAAGGATATGGGATTGGTGCAGTGTGTTCCCCATTG
TGTGTAATCCCCCCCAGTGACCCCGTGATGCTACCTGAGAATATCACTGCTGACACTCTG
GAGCACTGGATGGAACCTGTCAAAGTCCAGAGCATTGTGTGCACTGGCCGGCGTCAATGG
CACCCAGACCCCGTCTTAGTCCACTGCATCCAGTCATGTGAGCCCTTCCAAGCAGATGGT
TGGTGTGACACTATCAACAACCGAGCCTACTGCCACTATGACGGGGGAGACTGCTGCTCT
TCCACACTCTCCTCCAAGAAGGTCATTCCATTTGCTGCTGACTGTGACCTGGATGAGTGC
ACTTGCCGGGACCCCAAGGCAGAAGAAAATCAG
Protein sequence predicted based on nucleotide sequence
MMCLKILRISLAILAGWALCSANSELGWTRKKSLVEREHLNQVLLEGERCWLGAKVRRPR ASPQHHLFGVYPSRAGNYLRPYPVGEQEIHHTGRSKPDTEGNAVSLVPPDLTENPAGLRG AVEEPAAPWVGDSPIGQSELLGDDDAYLGNQRSKESLGEAGIQKGSAMAATTTTAIFTTL NEPKPETQRRGWAKSRQRRQVWKRRAEDGQGDSGISSHFQPWPKHSLKHRVKKSPPEESN QNGGEGSYREAETFNSQVGLPILYFSGRRERLLLRPEVLAEIPREAFTVEAWVKPEGGQN NPAIIAGVFDNCSHTVSDKGWALGIRSGKDKGKRDARFFFSLCTDRVKKATILISHSRYQ PGTWTHVAATYDGRHMALYVDGTQVASSLDQSGPLNSPFMASCRSLLLGGDSSEDGHYFR GHLGTLVFWSTALPQSHFQHSSQHSSGEEEATDLVLTASFEPVNTEWVPFRDEKYPRLEV LQGFEPEPEILSPLQPPLCGQTVCDNVELISQYNGYWPLRGEKVIRYQVVNICDDEGLNP IVSEEQIRLQHEALNEAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPEC EHPLTGYDGGDCRLQGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPQVADVRKTCFDPD SPKRAYMSVKELKEALQLNSTHFLNIYFASSVREDLAGAATWPWDKDAVTHLGGIVLSPA YYGMPGHTDTMIHEVGHVLGLYHVFKGVSERESCNDPCKETVPSMETGDLCADTAPTPKS ELCREPEPTSDTCGFTRFPGAPFTNYMSYTDDNCTDNFTPNQVARMHCYLDLVYQQWTES RKPTPIPIPPMVIGQTNKSLTIHWLPPISGVVYDRASGSLCGACTEDGTFRQYVHTASSR RVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPEVHLYHMNMTVPCPTEGCSLELLFQHP VQADTLTLWVTSFFMESSQVLFDTEILLENKESVHLGPLDTFCDIPLTIKLHVDGKVSGV KVYTFDERIEIDAALLTSQPHSPLCSGCRPVRYQVLRDPPFASGLPVVVTHSHRKFTDVE VTPGQMYQYQVLAEAGGELGEASPPLNHIHGAPYCGDGKVSERLGEECDDGDLVSGDGCS KVCELEEGFNCVGEPSLCYMYEGDGICEPFERKTSIVDCGIYTPKGYLDQWATRAYSSHE DKKKCPVSLVTGEPHSLICTSYHPDLPNHRPLTGWFPCVASENETQDDRSEQPEGSLKKE DEVWLKVCFNRPGEARAIFIFLTTDGLVPGEHQQPTVTLYLTDVRGSNHSLGTYGLSCQH NPLIINVTHHQNVLFHHTTSVLLNFSSPRVGISAVALRTSSRIGLSAPSNCISEDEGQNH QGQSCIHRPCGKQDSCPSLLLDHADVVNCTSIGPGLMKCAITCQRGFALQASSGQYIRPM QKEILLTCSSGHWDQNVSCLPVDCGVPDPSLVNYANFSCSEGTKFLKRCSISCVPPAKLQ GLSPWLTCLEDGLWSLPEVYCKLECDAPPIILNANLLLPHCLQDNHDVGTICKYECKPGY YVAESAEGKVRNKLLKIQCLEGGIWEQGSCIPVVCEPPPPVFEGMYECTNGFSLDSQCVL NCNQEREKLPILCTKEGLWTQEFKLCENLQGECPPPPSELNSVEYKCEQGYGIGAVCSPL CVIPPSDPVMLPENITADTLEHWMEPVKVQSIVCTGRRQWHPDPVLVHCIQSCEPFQADG WCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
