Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745226
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5580nts
Open reading frame: 1 to 5580
ATGGGAGCGGGAAGTGCCCGTGGTGCGCGGGGAACAGCAGCTGCCGCTGCTGCTAGAGGA
GGGGGTTTCTTGTTCTCGTGGATTTTGGTTAGCTTCGCATGTCACTTAGCGTCAACTCAG
GGTGCGCCCGAAGACGTCGACATACTCCAACGTCTAGGATTATCCTGGACAAAAGCCGGC
TCCCCTGCGCCGCCTGGTGTAATTCCATTTCAAAGCGGGTTTATCTTCACTCAGCGGGCG
CGACTTCAGGCTCCTACGGGAACTGTTATACCGGCAGCGCTGGGTACAGAACTCGCTCTA
GTGCTCTCGCTCTGCAGCCATCGAGTTAACCATGCTTTCCTCTTCGCCGTTCGCTCGCAG
AAGCGAAAACTGCAGCTCGGTCTACAATTTTTACCCGGTAAAACTGTTGTGCATCTAGGG
TCACGGCGGTCTGTTGCTTTTGATCTAGATATGCATGACGGCCGTTGGCATCATCTAGCC
CTAGAACTCCGGGGTAGGACCGTTACTCTCGTGACCGCTTGTGGTCAACGACGTGTACCC
GTCCTACTACCGTTTCACCGCGATCCCGCCTTGGATCCCGGAGGAAGTTTTTTATTTGGT
AAAATGAATCCACATGCTGTGCAATTTGAAGGCGCACTGTGTCAGTTTAGTATCTATCCT
GTCACACAAGTGGCGCATAACTATTGCACGCATTTGCGTAAACAATGTGGGCAAGCTGAC
ACATATCAAAGTCCCTTGGGCCCGCTCTTTTCTCAAGATAGTGGCAGACCCTTCACTTTT
CAGTCCGACCTAGCACTTTTGGGATTGGAGAACCTGACTACGGCAACTCCAGCCTTAGGG
AGCCTACCTGCCGGACGCGGGCCGCGCGGGACAGTGGCACCAGCTACTCCCACAAAGCCT
CAACGCACATCGCCGACCAACCCACATCAGCACATGGCTGTAGGAGGGCCAGCTCAAACA
CCGCTGTTGCCTGCGAAACTCTCCGCTTCTAATGCCTTGGACCCGATGCTACCAGCATCA
GTTGGCGGCTCGACCCGTACTCCCAGGCCGGCTGCCGCACAGCCCAGTCAAAAAATAACA
GCGACAAAGATACCGAAGAGTCTACCGACCAAGCCGAGCGCGCCATCTACGTCGATCGTA
CCGATCAAGAGCCCGCACCCGACCCAAAAAACAGCTCCATCAAGCTTTACCAAGAGTGCT
CTGCCCACACAGAAACAGGTACCTCCAACCTCCCGACCAGTACCGGCGAGAGTCAGCCGT
CCCGCGGAGAAGCCAATCCAAAGGAATCCGGGGATGCCTCGCCCCCCACCTCCGTCGACC
AGACCGCTACCGCCTACGACCTCAAGTTCAAAGAAACCGATTCCCACATTAGCGAGAACT
GAAGCCAAAATAACTTCACATGCCTCTAAACCGGCCAGCGCTAGAACATCTACTCATAAA
CCTCCCCCCTTTACTGCTTTGTCCTCTTCCCCAGCCCCCACTCCCGGAAGCACGAGATCT
ACACGCCCCCCGGCAACGATGGTTCCACCGACGTCAGGCACCTCTACTCCACGGACTGCA
CCCGCCGTGCCTACGCCAGGTTCTGCGCCGACCGGAAGTAAGAAACCAATCGGGTCAGAG
GCGTCTAAGAAGGCTGGCCCCAAATCGTCCCCTCGAAAACCCGTACCACTCAGGCCGGGT
AAGGCTGCTCGTGATGTGCCCCTATCAGATCTTACGACAAGACCCAGCCCGAGGCAACCG
CAGCCGAGCCAACAAACTACGCCTGCGCTGGTGCTTGCTCCAGCACAATTTCTGAGCTCT
TCTCCAAGACCTACGTCATCCGGATACTCTATCTTCCACCTAGCGGGATCAACGCCGTTC
CCATTGCTGATGGGACCTCCTGGCCCTAAAGGGGACTGTGGACTTCCTGGTCCTCCTGGG
CTCCCCGGACTGCCTGGGATACCAGGCGCTCGTGGGCCGCGTGGCCCACCAGGACCTTAT
GGCAATCCCGGACTACCAGGCCCGCCGGGTGCAAAGGGCCAGAAAGGTGATCCTGGCTTG
AGTCCAGGGAAAGCTCACGACGGAGCGAAAGGCGATATGGGGCTGCCCGGCCTCAGTGGA
AACCCCGGTCCGCCCGGTCGGAAGGGGCATAAGGGTTATCCCGGCCCGGCCGGTCATCCT
GGTGAGCAGGGACAGCCCGGCCCCGAGGGATCCCCCGGTGCGAAGGGTTACCCTGGACGT
CAAGGACTACCTGGTCCCGTTGGGGACCCTGGCCCAAAAGGATCGAGAGGGTACATTGGG
CTACCAGGGCTGTTCGGGCTCCCAGGGAGTGACGGAGAGCGAGGCTTGCCCGGGGTGCCG
GGAAAGCGGGGTAAGATGGGTATGCCTGGATTTCCAGGCGTGTTTGGCGAGCGGGGTCCT
CCAGGACTAGACGGAAATCCTGGCGAACTAGGCCTGCCGGGCCCTCCCGGCGTGCCTGGG
TTAATAGGAGATCTGGGAGTGCTGGGGCCGATCGGTTATCCGGGACCGAAGGGTATGAAA
GGGCTTATGGGATCGGTCGGAGAACCGGGTCTTAAGGGTGATAAAGGTGAACAAGGCGTA
CCGGGTGTTAGTGGAGATCCCGGGTTTCAAGGTGACAAAGGGTCCCAGGGGTTACCAGGC
TTCCCAGGAGCCCGGGGGAAGCCGGGTCCGCTCGGGAAGGTAGGAGACAAAGGCTCGATC
GGATTTCCCGGACCCCCAGGACCCGAGGGTTTCCCCGGAGATATTGGTCCGCCAGGCGAC
AATGGGCCGGAAGGCATGAAGGGTAAGCCCGGTGCCCGCGGATTACCCGGACCGCGTGGG
CAGCTTGGCCCAGAGGGCGACGAAGGACCCATGGGGCCACCTGGTGCGCCGGGACTTGAA
GGTCAACCGGGCCGTAAAGGGTTCCCGGGGCGTCCTGGATTAGACGGCGTAAAAGGGGAA
CCCGGGGATCCAGGTCGGCCGGGACCCGTAGGCGAACAGGGCTTCATGGGTTTTATTGGT
TTGGTGGGTGAGCCAGGAATAGTTGGCGAGAAGGGTGACCGAGGAATGATGGGGCCTCCG
GGCGTTCCAGGTCCAAAGGGGTCCATGGGACACCCCGGTATGCCCGGTGGGATGGGAACA
CCGGGAGAGCCGGGGCCCCAAGGGCCACCAGGTTCCAGGGGGCCGCCCGGGATGAGGGGT
GCGAAAGGTCGTCGTGGTCCAAGAGGGCCTGATGGCCCCGCTGGAGAGCAGGGCAGCAGA
GGATTGAAAGGCCCACCTGGGCCTCAGGGCAGGCCAGGAAGACCAGGACAACAAGGTGTG
GCGGGTGAGAGAGGTCATTTGGGGTCAAGGGGTTTTCCTGGTATACCGGGCCCCTCCGGC
CCCCCCGGAACAAAGGGGCTACCCGGAGAGCCCGGTCCTCAAGGCCCTCAAGGACCGATT
GGACCTCCGGGTGAAATGGGACCCAAAGGTCCGCCGGGGGCGGTAGGTGAACCAGGTTTA
CCTGGTGAAGCAGGAATGAAAGGAGATTTAGGTCCATTAGGCACACCGGGCGAACAAGGG
CTAATCGGACAGCGAGGAGAGCCTGGACTTGAGGGGGACTCAGGACCTATGGGCCCGGAT
GGCCTTAAGGGGGATCGAGGAGATCCTGGGCCCGATGGGGAGCATGGTGAAAAAGGTCAA
GAAGGACTCATGGGGGAGGATGGTCCACCTGGACCCCCTGGTGTTACCGGCGTTCGGGGA
CCGGAGGGTAAAAGCGGTAAACAGGGAGAAAAAGGAAGAACAGGAGCTAAGGGTGCTAAG
GGGTATCAAGGACAATTGGGCGAAATGGGCGTACCCGGCGATCCAGGCCCCCCAGGGACG
CCAGGCCCAAAGGGTAGTCGTGGTTCCTTGGGTCCTACTGGTGCCCCAGGGCGTATGGGC
GCTCAAGGCGAGCCCGGCTTGGCCGGATATGACGGGCACAAGGGTATCGTGGGACCCCTC
GGCCCACCCGGTCCCAAAGGCGAGAAAGGGGAGCAGGGGGAAGATGGGAAGGCGGAGGGA
CCGCCCGGACCACCTGGCGACAGGGGCCCTGTAGGTGATCGTGGCGACCGTGGTGAGCCT
GGTGATCCGGGATACCCGGGACAGGAGGGAGTACAAGGCCTTCGCGGTAAGCCTGGGCAG
CAAGGGCAACCTGGGCACCCAGGTCCCCGTGGCTGGCCTGGCCCGAAAGGTTCAAAAGGC
GCTGAAGGTCCTAAGGGAAAACAAGGAAAAGCAGGGGCACCTGGTCGTCGCGGTGTACAG
GGGCTTCAAGGGCTGCCAGGACCAAGGGGAGTGGTTGGGCGCCAGGGGCTAGAGGGTATT
GCCGGCCCAGATGGGTTACCGGGACGGGATGGTCAGGCTGGGCAACAGGGTGAGCAAGGG
GATGACGGTGATCCAGGGCCTATGGGACCAGCAGGGAAACGGGGCAACCCAGGTGTAGCT
GGCCTCCCAGGTGCTCAGGGTCCACCCGGATTTAAAGGAGAATCTGGCTTGCCTGGTCAA
CTCGGTCCTCCCGGGAAGAGAGGTACTGAGGGTCGCACAGGGCTCCCTGGCAACCAAGGC
GAACCGGGCAGTAAGGGCCAACCCGGTGACAGTGGAGAGATGGGGTTCCCTGGTATGGCC
GGGTTGTTTGGTCCGAAAGGACCTCCAGGTGATATTGGGTTCAAAGGCATTCAGGGCCCG
CGAGGGCCGCCGGGCCTTATGGGTAAGGAGGGTATAGTGGGTCCGTTGGGTATACTCGGC
CCCTCTGGTCTGCCTGGACCAAAGGGCGACAAGGGATCACGAGGTGATTGGGGGTTACAA
GGACCACGTGGGCCCCCAGGCCCTAGAGGGCGTCCCGGGCCACCCGGCCCTCCAGGCGGA
CCCATCCAACTACAGCAGGATGATCTGGGCGCTGCGTTCCAAACGTGGATGGATACGTCC
GGAGCTCTGCGTCCAGAGAGCTACAGTTATCCTGACCGGCTTGTCTTAGATCAGGGTGGC
GAAATTTTCAAAACGCTTCATTATCTCTCGAACCTTATACAAAGCATTAAGACGCCGCTT
GGAACTAAAGAAAATCCCGCAAGAGTATGCCGAGACTTAATGGATTGCGAACAAAAAATG
GTTGACGGCACTTACTGGGTGGATCCAAACCTAGGATGTTCAAGTGACACTATTGAGGTA
TCATGTAACTTTACACACGGAGGGCAAACGTGCTTAAAACCAATTACCGCTAGCAAGGTT
GAATTCGCCATTAGTCGTGTCCAAATGAACTTTCTACATTTGTTGTCTTCGGAGGTAACG
CAACACATAACCATACATTGCCTAAATATGACTGTTTGGCAAGAGGGAACGGGACAGACT
CCAGCGAAGCAAGCCGTGCGATTTCGAGCATGGAACGGGCAAATCTTCGAAGCTGGAGGC
CAATTCCGTCCAGAAGTCAGCATGGACGGATGCAAAGTCCAAGATGGTCGATGGCATCAG
ACGCTTTTTACTTTCAGGACACAAGATCCGCAACAATTACCCATTATTTCCGTTGACAAT
TTGCCGCCCGCATCCAGTGGGAAGCAGTACCGCCTTGAAGTCGGTCCTGCCTGTTTTCTG
Protein sequence predicted based on nucleotide sequence
MGAGSARGARGTAAAAAARGGGFLFSWILVSFACHLASTQGAPEDVDILQRLGLSWTKAG SPAPPGVIPFQSGFIFTQRARLQAPTGTVIPAALGTELALVLSLCSHRVNHAFLFAVRSQ KRKLQLGLQFLPGKTVVHLGSRRSVAFDLDMHDGRWHHLALELRGRTVTLVTACGQRRVP VLLPFHRDPALDPGGSFLFGKMNPHAVQFEGALCQFSIYPVTQVAHNYCTHLRKQCGQAD TYQSPLGPLFSQDSGRPFTFQSDLALLGLENLTTATPALGSLPAGRGPRGTVAPATPTKP QRTSPTNPHQHMAVGGPAQTPLLPAKLSASNALDPMLPASVGGSTRTPRPAAAQPSQKIT ATKIPKSLPTKPSAPSTSIVPIKSPHPTQKTAPSSFTKSALPTQKQVPPTSRPVPARVSR PAEKPIQRNPGMPRPPPPSTRPLPPTTSSSKKPIPTLARTEAKITSHASKPASARTSTHK PPPFTALSSSPAPTPGSTRSTRPPATMVPPTSGTSTPRTAPAVPTPGSAPTGSKKPIGSE ASKKAGPKSSPRKPVPLRPGKAARDVPLSDLTTRPSPRQPQPSQQTTPALVLAPAQFLSS SPRPTSSGYSIFHLAGSTPFPLLMGPPGPKGDCGLPGPPGLPGLPGIPGARGPRGPPGPY GNPGLPGPPGAKGQKGDPGLSPGKAHDGAKGDMGLPGLSGNPGPPGRKGHKGYPGPAGHP GEQGQPGPEGSPGAKGYPGRQGLPGPVGDPGPKGSRGYIGLPGLFGLPGSDGERGLPGVP GKRGKMGMPGFPGVFGERGPPGLDGNPGELGLPGPPGVPGLIGDLGVLGPIGYPGPKGMK GLMGSVGEPGLKGDKGEQGVPGVSGDPGFQGDKGSQGLPGFPGARGKPGPLGKVGDKGSI GFPGPPGPEGFPGDIGPPGDNGPEGMKGKPGARGLPGPRGQLGPEGDEGPMGPPGAPGLE GQPGRKGFPGRPGLDGVKGEPGDPGRPGPVGEQGFMGFIGLVGEPGIVGEKGDRGMMGPP GVPGPKGSMGHPGMPGGMGTPGEPGPQGPPGSRGPPGMRGAKGRRGPRGPDGPAGEQGSR GLKGPPGPQGRPGRPGQQGVAGERGHLGSRGFPGIPGPSGPPGTKGLPGEPGPQGPQGPI GPPGEMGPKGPPGAVGEPGLPGEAGMKGDLGPLGTPGEQGLIGQRGEPGLEGDSGPMGPD GLKGDRGDPGPDGEHGEKGQEGLMGEDGPPGPPGVTGVRGPEGKSGKQGEKGRTGAKGAK GYQGQLGEMGVPGDPGPPGTPGPKGSRGSLGPTGAPGRMGAQGEPGLAGYDGHKGIVGPL GPPGPKGEKGEQGEDGKAEGPPGPPGDRGPVGDRGDRGEPGDPGYPGQEGVQGLRGKPGQ QGQPGHPGPRGWPGPKGSKGAEGPKGKQGKAGAPGRRGVQGLQGLPGPRGVVGRQGLEGI AGPDGLPGRDGQAGQQGEQGDDGDPGPMGPAGKRGNPGVAGLPGAQGPPGFKGESGLPGQ LGPPGKRGTEGRTGLPGNQGEPGSKGQPGDSGEMGFPGMAGLFGPKGPPGDIGFKGIQGP RGPPGLMGKEGIVGPLGILGPSGLPGPKGDKGSRGDWGLQGPRGPPGPRGRPGPPGPPGG PIQLQQDDLGAAFQTWMDTSGALRPESYSYPDRLVLDQGGEIFKTLHYLSNLIQSIKTPL GTKENPARVCRDLMDCEQKMVDGTYWVDPNLGCSSDTIEVSCNFTHGGQTCLKPITASKV EFAISRVQMNFLHLLSSEVTQHITIHCLNMTVWQEGTGQTPAKQAVRFRAWNGQIFEAGG QFRPEVSMDGCKVQDGRWHQTLFTFRTQDPQQLPIISVDNLPPASSGKQYRLEVGPACFL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
