Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745259
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5310nts
Open reading frame: 1 to 5310
ATGTCGGGAGCCTCAGTGAAGGTGGCTGTCCGGGTAAGGCCCTTCAATTCTCGAGAGACT
AGCAAGGAATCCAAATGCATCATTCAGATGCAAGGCAACTCGACCAGTATTATTAACCCA
AAGAATCCAAAGGAAGCTCCAAAGTCCTTCAGCTTCGACTATTCCTACTGGTCGCATACC
TCACCCGAAGATCCCTGTTTTGCATCTCAAAACCGTGTGTACAATGACATTGGCAAGGAA
ATGCTCTTACACGCCTTTGAGGGATATAATGTCTGTATTTTTGCCTATGGGCAGACTGGT
GCTGGAAAATCTTATACAATGATGGGTAAACAAGAAGAAAGCCAGGCTGGCATCATTCCA
CAGTTATGTGAAGAACTTTTTGAGAAAATCAATGACAACTGTAATGAAGAAATGTCTTAC
TCTGTAGAGGTGAGCTACATGGAAATTTACTGTGAAAGAGTACGAGATTTGCTGAATCCA
AAAAACAAGGGTAATTTGCGTGTGCGTGAACACCCACTTCTTGGACCCTATGTGGAGGAT
CTGTCCAAGTTGGCAGTTACTTCCTACACAGACATTGCTGACCTCATGGATGCTGGGAAC
AAAGCCAGGACAGTGGCAGCTACAAACATGAATGAAACAAGTAGCCGTTCCCACGCTGTG
TTTACGATTGTTTTCACCCAGAAGAAACACGATAATGAGACTAACCTTTCCACTGAGAAG
GTCAGTAAAATCAGCTTGGTGGATCTAGCAGGAAGTGAACGAGCTGATTCAACTGGTGCC
AAAGGGACTCGATTAAAGGAAGGAGCAAATATTAATAAGTCTCTTACAACTTTGGGCAAA
GTCATTTCAGCCTTGGCCGAGGTGAGTAAAAAGAAGAAGAAAACAGATTTTATTCCCTAC
AGGGATTCTGTACTTACTTGGCTCCTTCGAGAAAATTTAGGTGGCAATTCTCGGACTGCA
ATGGTTGCTGCTCTGAGCCCCGCGGATATCAACTACGATGAGACTTTGAGCACTCTGAGA
TATGCAGATCGTGCAAAACAAATTAAATGCAATGCTGTTATCAATGAGGACCCCAATGCC
AAACTGGTTCGTGAATTAAAGGAGGAGGTGACACGGCTGAAGGACCTTCTTCGTGCTCAG
GGCCTGGGAGATATTATTGATACATCCATGGGGTCCCTCACTTCATCCCCATCTTCCTGC
TCACTCAGTAGTCAGGTGGGCTTGACGTCTGTGACCAGTATTCAAGAGAGGATCATGTCT
ACACCTGGAGGAGAGGAAGCTATTGAACGTTTAAAGGAATCAGAGAAGATCATTGCTGAG
TTGAATGAAACTTGGGAAGAGAAGCTTCGTAAAACAGAGGCCATCAGAATGGAGAGAGAG
GCTTTGTTGGCTGAGATGGGAGTTGCCATTCGGGAAGATGGAGGAACCCTAGGGGTTTTC
TCACCTAAAAAGACCCCACATCTTGTTAACCTCAATGAAGACCCACTAATGTCTGAGTGC
CTACTTTATTACATCAAAGATGGAATTACAAGGGTTGGCCAAGCAGATGCTGAGCGGCGC
CAGGACATAGTGCTGAGCGGGGCTCACATTAAAGAAGAGCATTGTATCTTCCGGAGTGAG
AGAAGCAACAGCGGGGAAGTTATCGTGACCTTAGAGCCCTGTGAGCGCTCAGAAACCTAC
GTAAATGGCAAGAGGGTGTCCCAGCCTGTTCAGCTGCGCTCAGGAAACCGTATCATCATG
GGTAAAAACCATGTTTTCCGCTTTAACCACCCGGAACAAGCACGAGCTGAGCGAGAGAAG
ACTCCTTCTGCTGAGACACCCTCTGAGCCTGTGGACTGGACATTTGCCCAGAGGGAGCTT
CTGGAAAAACAAGGAATTGATATGAAACAAGAGATGGAGAAAAGGCTACAGGAAATGGAG
ATCTTATACAAAAAGGAGAAGGAAGAAGCAGATCTTCTTTTGGAGCAGCAGAGACTGGAC
TATGAGAGTAAATTGCAGGCCTTGCAGAAGCAGGTTGAAACCCGATCTCTGGCTGCAGAA
ACAACTGAAGAGGAGGAAGAAGAGGAAGAAGTTCCTTGGACACAGCATGAATTTGAGTTG
GCCCAATGGGCCTTCCGGAAATGGAAGTCTCATCAGTTTACTTCATTACGGGACTTACTC
TGGGGCAATGCCGTGTACCTAAAGGAGGCCAATGCCATCAGTGTGGAACTGAAAAAGAAG
GTGCAGTTTCAGTTTGTTCTGCTGACTGACACACTGTACTCCCCTTTGCCTCCTGAATTA
CTTCCCACTGAGATGGAAAAAACTCATGAGGACAGGCCTTTCCCTCGCACAGTGGTAGCA
GTAGAAGTCCAGGATTTGAAGAATGGAGCAACACACTATTGGTCTTTGGAGAAACTCAAG
CAGAGGCTGGATTTGATGCGAGAGATGTATGATAGGGCAGGGGAGATGGCCTCCAGTGCC
CAAGACGAAAGCGAAACCACTGTGACTGGCAGCGATCCCTTCTATGATCGGTTCCACTGG
TTCAAACTTGTGGGGAGCTCCCCCATTTTCCACGGCTGTGTGAACGAGCGCCTTGCCGAC
CGCACACCCTCCCCCACTTTTTCCACGGCCGATTCCGACATCACTGAGCTGGCTGACGAG
CAGCAAGATGAGATGGAGGATTTTGATGATGAGGCATTCGTGGATGACGCCGGCTCTGAC
GCAGGGACGGAGGAGGGATCAGATCTCTTCAGTGACGGGCATGACCCGTTTTACGACCGA
TCCCCTTGGTTCATTTTAGTGGGAAGGGCATTTGTTTACCTGAGCAATCTGCTGTATCCC
GTGCCCCTGATCCACAGGGTGGCCATCGTCAGTGAGAAAGGTGAAGTGCGGGGATTTCTG
CGTGTGGCTGTACAGGCCATCGCAGCGGATGAAGAAGCTCCTGATTATGGCTCTGGAATT
CGACAGTCAGGAACAGCTAAAATATCTTTTGATAATGAATACTTTAATCAGAGTGACTTT
TCGTCTGTTGCAATGACTCGTTCTGGTCTGTCCTTGGAGGAGTTGAGGATTGTGGAAGGA
CAGGGTCAGAGTTCTGAGGTCATCACTCCTCCAGAAGAAATCAGTCGAATTAATGACTTG
GATTTGAAGTCAAGCACTTTGCTGGATGGTAAGATGGTAATGGAAGGGTTTTCTGAAGAG
ATTGGCAACCACCTGAAACTGGGCAGTGCCTTCACTTTCCGAGTAACAGTGTTGCAGGCC
AGTGGAATCCTCCCAGAGTATGCAGATATCTTCTGTCAGTTCAACTTTTTGCATCGCCAT
GATGAAGCATTCTCCACGGAGCCCCTCAAAAACAATGGCAGAGGAAGTCCCCTGGCCTTT
TATCATGTGCAGAATATTGCAGTGGAGATCACTGAATCATTTGTGGATTACATCAAAACC
AAGCCTATTGTATTTGAAGTCTTTGGGCATTATCAGCAGCACCCACTTCATCTGCAAGGA
CAGGAGCTTAACAGTCCGCCTCAGCCGTGCCGCCGATTCTTCCCTCCACCCATGCCACTG
TCCAAGCCAGTTCCAGCCACCAAGTTAAACACGATGAGCAAAACCAGCCTTGGCCAGAGC
ATGAGCAAGTATGACCTCCTGGTTTGGTTTGAGATCAGTGAACTGGAGCCTACAGGAGAG
TATATCCCAGCTGTGGTTGACCACACAGCAGGCTTGCCTTGCCAGGGGACATTTTTGCTT
CATCAGGGCATCCAGCGAAGGATCACAGTGACCATTATCCATGAGAAGGGGAGCGAGCTC
CATTGGAAAGATGTTCGTGAACTGGTGGTAGGTCGTATTCGGAATAAGCCTGAGGTGGAT
GAAGCTGCAGTTGATGCCATCCTCTCCCTAAATATTATTTCTGCCAAGTACCTGAAGTCT
TCCCACAACTCTAGCAGGACCTTCTACCGCTTTGAGGCTGTGTGGGATAGCTCTCTGCAT
AACTCCCTTCTTCTGAACCGAGTGACACCCTATGGAGAAAAGATCTACATGACCTTGTCG
GCCTACCTAGAGCTGGATCATTGCATCCAGCCGGCTGTCATCACCAAGGATGTGTGCATG
GTCTTCTACTCCCGAGATGCCAAGATCTCACCACCACGCTCTCTGCGTAGCCTCTTTGGC
AGCGGCTACTCAAAGTCACCAGATTCGAATCGAGTCACTGGCATTTACGAACTCAGCTTA
TGCAAAATGTCAGACACAGGTAGTCCAGGTATGCAGAGAAGGAGAAGAAAAATCTTAGAT
ACGTCAGTGGCATATGTGCGGGGAGAAGAGAACTTAGCAGGCTGGCGGCCCCGTGGAGAC
AGCCTCATCCTTGAGCACCAGTGGGAGCTGGAGAAGCTGGAGCTCCTACATGAGGTGGAA
AAAACCCGCCACTTTTTGCTGCTGCGTGAGAGACTTGGTGACAGCATCCCCAAATCCCTG
AGCGACTCGTTATCCCCCAGCCTCAGCAGTGGGACCCTCAGCACCTCCACCAGTATCTCC
TCTCAGATCTCAACCACTACCTTTGAAAGCGCCATCACACCTAGCGAGAGCAGTGGCTAT
GATTCAGGAGACATCGAAAGCCTGGTGGACCGAGAGAAAGAGCTGGCTACCAAGTGCCTG
CAACTTCTCACCCACACTTTCAACAGAGAATTCAGCCAGGTGCACGGCAGCGTCAGTGAC
TGTAAGTTGTCTGATATCTCTCCAATTGGACGGGATCCCTCTGAGTCCAGTTTCAGCAGT
GCCACCCTCACTCCCTCCTCCACCTGTCCCTCTCTGGTAGACTCTAGGAGCAACTCTCTG
GATCAGAAGACCCCAGAAGCCAATTCCCGGGCCTCTAGTCCCTGCCCAGAATTTGAACAG
TTTCAGATTGTCCCAGCTGTGGAAACACCATATTTGGCCCGAGCAGGAAAAAACGAATTT
CTCAATCTTGTTCCAGATATTGAAGAAATTAGACCAAGCTCAGTGGTGTCTAAGAAAGGA
TACCTTCATTTCAAGGAGCCTCTTTACAGTAACTGGGCTAAACATTTTGTTGTCGTCCGT
CGGCCTTATGTCTTCATCTATAACAGTGACAAAGACCCTGTGGAGCGTGGAATCATTAAC
CTGTCCACAGCACAGGTGGAGTACAGTGAGGACCAGCAGGCCATGGTGAAGACACCAAAC
ACCTTTGCTGTCTGCACAAAGCACCGTGGGGTCCTTTTGCAGGCCCTCAATGACAAAGAC
ATGAACGACTGGTTGTATGCCTTCAACCCACTTCTAGCTGGCACAATACGGTCAAAGCTT
TCCCGCAGATGCCCGAGCCAGTCGAAATAC
Protein sequence predicted based on nucleotide sequence
MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHT SPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIP QLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVED LSKLAVTSYTDIADLMDAGNKARTVAATNMNETSSRSHAVFTIVFTQKKHDNETNLSTEK VSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPY RDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDPNA KLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLSSQVGLTSVTSIQERIMS TPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVF SPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSE RSNSGEVIVTLEPCERSETYVNGKRVSQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREK TPSAETPSEPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQRLD YESKLQALQKQVETRSLAAETTEEEEEEEEVPWTQHEFELAQWAFRKWKSHQFTSLRDLL WGNAVYLKEANAISVELKKKVQFQFVLLTDTLYSPLPPELLPTEMEKTHEDRPFPRTVVA VEVQDLKNGATHYWSLEKLKQRLDLMREMYDRAGEMASSAQDESETTVTGSDPFYDRFHW FKLVGSSPIFHGCVNERLADRTPSPTFSTADSDITELADEQQDEMEDFDDEAFVDDAGSD AGTEEGSDLFSDGHDPFYDRSPWFILVGRAFVYLSNLLYPVPLIHRVAIVSEKGEVRGFL RVAVQAIAADEEAPDYGSGIRQSGTAKISFDNEYFNQSDFSSVAMTRSGLSLEELRIVEG QGQSSEVITPPEEISRINDLDLKSSTLLDGKMVMEGFSEEIGNHLKLGSAFTFRVTVLQA SGILPEYADIFCQFNFLHRHDEAFSTEPLKNNGRGSPLAFYHVQNIAVEITESFVDYIKT KPIVFEVFGHYQQHPLHLQGQELNSPPQPCRRFFPPPMPLSKPVPATKLNTMSKTSLGQS MSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSEL HWKDVRELVVGRIRNKPEVDEAAVDAILSLNIISAKYLKSSHNSSRTFYRFEAVWDSSLH NSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFG SGYSKSPDSNRVTGIYELSLCKMSDTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGD SLILEHQWELEKLELLHEVEKTRHFLLLRERLGDSIPKSLSDSLSPSLSSGTLSTSTSIS SQISTTTFESAITPSESSGYDSGDIESLVDREKELATKCLQLLTHTFNREFSQVHGSVSD CKLSDISPIGRDPSESSFSSATLTPSSTCPSLVDSRSNSLDQKTPEANSRASSPCPEFEQ FQIVPAVETPYLARAGKNEFLNLVPDIEEIRPSSVVSKKGYLHFKEPLYSNWAKHFVVVR RPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKD MNDWLYAFNPLLAGTIRSKLSRRCPSQSKY
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
