Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745273
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5478nts
Open reading frame: 1 to 5478
ATGGGGGACTCCAAAGTGAAAGTGGCGGTGCGGATACGACCCATGAACCGGCGAGAGACT
GACTTGCATACCAAATGTGTGGTGGATGTGGATGCAAACAAGGTTATTCTTAATCCTGTA
AATACGAATCTTTCCAAAGGAGATGCCCGGGGCCAGCCGAAGGTGTTTGCTTATGATCAT
TGTTTCTGGTCTATGGATGAATCCGTCAAAGAAAAGTATGCAGGTCAAGATATTGTTTTC
AAGTGCCTTGGAGAGAATATCCTGCAGAATGCTTTTGATGGCTACAATGCATGTATCTTT
GCCTATGGACAGACTGGCTCCGGAAAATCTTATACCATGATGGGCACAGCCGACCAACCT
GGATTAATTCCAAGACTTTGCAGCGGACTCTTTGAACGAACTCAGAAAGAGGAAAATGAA
GAACAGAGCTTTAAAGTAGAAGTGTCCTACATGGAAATCTATAATGAAAAAGTTCGCGAC
CTTCTTGATCCTAAAGGTAGCCGTCAGACGCTGAAAGTCAGAGAGCATAGTGTCTTGGGA
CCTTATGTCGACGGACTTTCTAAACTGGCTGTGACAAGCTACAAGGATATTGAGTCTTTG
ATGTCTGAGGGTAACAAATCTCGCACTGTTGCTGCAACCAACATGAACGAGGAGAGTAGC
CGATCCCATGCAGTTTTCAAAATCACATTGACACATACTCTGTACGATGTGAAGTCTGGG
ACATCTGGAGAGAAAGTGGGCAAACTCAGCCTGGTGGATTTAGCTGGCAGTGAACGAGCA
ACGAAGACAGGCGCTGCAGGGGACAGGTTGAAGGAAGGGAGCAACATTAACAAGTCCCTC
ACAACCCTCGGTTTGGTTATCTCAGCTCTTGCAGATCAGAGTGCTGGCAAAAACAAGAAT
AAATTTGTTCCATATCGGGACTCAGTTCTCACCTGGCTGCTCAAAGACAGCCTCGGGGGT
AACAGTAAGACCGCCATGGTGGCTACTGTGAGTCCTGCAGCTGATAACTATGATGAAACC
CTCTCAACTCTGCGGTATGCAGATAGAGCCAAGCACATTGTAAACCACGCTGTGGTGAAT
GAGGACCCTAATGCCCGAATCATCCGCGATCTCCGGGAAGAAGTTGAGAAACTCCGGGAG
CAGCTGACCAAAGCAGAGGCAATGAAATCTCCAGAACTAAAGGACCGGCTGGAAGAATCT
GAGAAGCTAATCCAGGAAATGACTGTGACCTGGGAGGAGAAATTAAGGAAAACGGAGGAG
ATTGCACAGGAAAGACAGAAACAGCTTGAGAGTCTTGGAATATCTCTTCAGTCTTCGGGA
ATCAAAGTTGGGGATGATAAATGCTTCCTGGTGAATCTGAATGCTGACCCAGCCCTGAAT
GAGCTTTTGGTGTACTATTTAAAGGAACATACATTGATAGGCTCAGCAAATTCCCAAGAT
ATCCAACTGTGCGGCATGGGAATTCTTCCAGAACACTGTATTATAGACATCACGTCAGAA
GGCCAGGTTATGCTGACTCCTCAGAAGAACACCAGAACATTTGTAAATGGGTCATCTGTC
TCCAGTCCAATACAGCTACACCATGGGGACAGGATATTATGGGGAAACAATCATTTCTTC
AGGCTCAATTTGCCTAAAAAGAAAAAGAAAGCAGAACGAGAGGATGAGGACCAGGATCCC
TCCATGAAGAACGAGAATAGTTCTGAGCAGCTCGATGTAGACGGAGACTCCTCCAGCGAG
GTGTCCAGCGAAGTTAACTTTAATTACGAATACGCACAGATGGAGGTCACGATGAAGGCC
CTCGGCAGCAATGATCCGATGCAGTCCATATTAAACAGCTTAGAACAACAGCATGAAGAA
GAAAAACGATCAGCACTGGAGCGCCAGAGGCTTATGTATGAGCACGAATTGGAGCAGCTC
CGGAGAAGGCTGTCTCCTGAGAAGCAGAACTGCCGGAGCATGGACAGGTTTTCTTTCCAC
TCGCCCAGCGCTCAGCAACGCTTAAGACAGTGGGCTGAGGAGAGGGAAGCAACGCTGAAT
AACAGCCTGATGAGGCTGAGGGAACAAATTGTTAAGGCCAATCTATTGGTGAGAGAAGCT
AATTACATTGCTGAGGAGCTGGATAAAAGAACAGAATACAAAGTTACCCTACAGATTCCA
GCATCCAGCCTGGATGCCAACAGGAAGCGAGGCTCTCTTCTTTCCGAGCCTGCAATCCAG
GTGAGAAGAAAAGGAAAAGGAAAGCAGATTTGGTCTTTGGAAAAACTGGACAACAGGCTG
TTGGATATGAGAGATCTTTATCAGGAGTGGAAAGAGTGTGAAGAAGATAACCCAGTAATA
CGATCATACTTCAAACGTGCTGATCCATTCTATGATGAGCAGGAAAATCACAGTCTCATT
GGGGTGGCCAATGTCTTCCTGGAGTCACTTTTCTATGATGTGAAGTTACAATACGCTGTT
CCCATCATCAACCAGAAAGGAGAGGTGGCAGGTCGGCTGCACGTGGAGGTGATGCGACTC
AGTGGTGATGTGGGGGAGAGGATCGCAGGAGGCGATGAGGTGGCAGAGGTCAGCTTTGAG
AAGGAAACCCAGGAGAACAAACTCGTGTGCATGGTTAAAATCCTGCAAGCTACTGGGTTG
CCACAGCATCTTTCCCACTTTGTTTTCTGCAAATACAGCTTCTGGGATCAACAGGAGCCG
GTGATTGTCGCTCCTGAAGTGGACACCTCCTCCTCTTCCGTCAGCAAGGAGCCGCACTGC
ATGGTTGTCTTTGATCATTGCAATGAGTTTTCCGTTAACATCACCGAAGACTTTATCGAG
CATCTTTCCGAAGGAGCATTGGCAATTGAAGTATATGGACACAAAATAAACGATCCCCGA
AAAAACCCCGCCCTGTGGGATTTGGGAATCATCCAAGCAAAGACACGTAGTCTAAGGGAC
AGATGGAGTGAAGTGACCAGGAAATTGGAATTTTGGGTTCAAATCTTGGAACAGAATGAG
AATGGTGAATACTGCCCTGTAGAAGTGATTTCTGCAAAGGATGTCCCAACCGGAGGAATC
TTCCAGCTGCGGCAGGGGCAGTCCCGGAGAGTTCAAGTCGAAGTGAAGTCCGTGCAGGAA
TCCGGGACTTTACCACTGATGGAAGAATGTATACTGTCTGTTGGCATTGGATGTGTCAAA
GTAAGACCGCTCAGAGCCCCCAGAACACATGAAACCTTCCATGAGGAAGAGGAAGACATG
GACAGCTACCAGGATCGAGATTTAGAGAGACTTCGTAGAAAATGGCTGAATGCATTGACA
AAACGTCAGGAGTACTTGGATCAACAATTGCAAAAGCTTGTCAGTAAACGTGATAAAACA
GAGGATGATGCTGACCGCGAAGCGCAGCTGCTGGAGATGCGGTTGACCCTAACTGAGGAG
AGGAACGCGGTGATGGTCCCCTCTGCTGGCAGTGGTATTCCAGGGGCCCCAGCAGAATGG
ACCCCCGTACCTGGGATGGAGACACACATTCCAGTGATATTCCTGGACTTAAATGCTGAT
GATTTCAGCTCTCAGGATAATCTTGATGACCCAGAAGCTGGTGGATGGGATGCGACCTTG
ACTGGGGAAGAAGAAGAGGAGTTCTTTGAATTGCAGATAGTGAAGCAGCATGATGGGGAG
GTGAAAGCAGAAGCCTCCTGGGACTCCGCAGTGCATGGCTGCCCTCAGCTTAGCAGGGGC
ACTCCCGTAGACGAGCGGTTGTTCCTTATCGTACGCGTGACGGTCCAGCTCAGCCACCCT
GCTGACATGCAACTGGTGTTACGAAAGAGAATCTGTGTCAATGTTCACGGCCGCCAGGGT
TTTGCACAGAGTCTCCTAAAAAAGATGTCGCATCGAAGTTCTATTCCTGGCTGTGGAGTG
ACTTTTGAAATTGTCTCCAATATTCCAGAGGATGCCCAGGGAGTGGAAGAACGGGAAGCA
TTAGCAAGAATGGCAGCCAATGTTGAAAACCCAGCTTCTGCTGACTCGGAGGCTTATATT
GAAAAGTACCTCAGGAGCGTGCTCGCTGTAGAAAACCTCCTGACTCTAGATCGTCTGCGC
CAGGAAGTTGCAGTGAAGGAACAGTTAACAGGAAAAGGAAAGTTGAGCAGGAGGAGTATC
AGTTCTCCAAATGTGAACAGATTGTCTGGAAGCCGTCAAGATCTCATCCCATCCTACAGT
CTAGGCAGCAACAAGGGCCGGTGGGAAAGTCAGCAGGATGTATCCCAAACCACAGTTTCC
AGAGGAATAGCTCCTGCCCCTGCCCTGTCTGTTTCTCCACAAAATAACCATTCTCCAGAT
CCAGGACTCAGCAACCTTGCAGCATCCTACTTAAATCCTGTCAAATCCTTCGTGCCGCAA
ATGCCAAAGCTCCTCAAGTCTCTCTTTCCCGTCCGCGATGAGAAGAGGGGCAAGAGGCCT
TCTCCCCTTGCACACCAGCCCGTGCCCCGCATCATGGTTCAGTCAGCTAGCCCGGACATA
AGGGTGACCAGGATGGAGGAGGCTCAGCCTGAGATGGGCCCTGATGTGCTGGTGCAGACG
ATGGGGGCACCGGCCTTGAAGATCTGCGACAAACCTGCCAAAGTGCCTTCCCCACCACCT
GTGATAGCTGTCACAGCGGTCACCCCGGCTCCGGAAGCACAGGACGGACCCCCAAGCCCA
TTGAGTGAAGCCTCTAGCGGGTACTTCTCCCACAGCGTATCTACCGCTACCCTGTCTGAT
GCCTTGGGCCCAGGCTTAGACGCTGCGGCCCCACCTGGATCCATGCCAACTGCTCCTGAA
GCAGAGCCCGAGGCGCCAATTTCCCATCCACCACCACCTACAGCTGTCCCTGCAGAAGAG
CCTCCCGGACCTCAGCAGCTCGTAAGTCCCGGTAGAGAGCGACCCGACTTAGAAGCTCCA
GCTCCTGGATCACCGTTCCGCGTCAGAAGGGTGCGTGCCTCGGAATTGCGTTCATTTTCG
CGTATGCTGGCTGGTGATCCTGGCTGCTCTCCGGGAGCTGAGGGGAATGCGCCAGCCCCT
GGAGCAGGGGGACAAGCCCTAGCCTCTGATTCTGAAGAAGCTGATGAAGTCCCGGAGTGG
CTCCGAGAGGGCGAGTTCGTCACCGTGGGAGCCCACAAAACTGGCGTGGTGAGATACGTT
GGACCTGCCGACTTCCAAGAGGGCACGTGGGTCGGCGTTGAGCTCGACCTGCCCTCAGGT
AAGAATGACGGTTCCATCGGCGGGAAGCAGTACTTCCGGTGTAACCCTGGCTACGGGCTA
CTTGTTAGACCCAGTCGGGTTCGTAGGGCCACAGGACCTGTGCGGAGGCGTAGCACAGGA
CTACGACTGGGTGCTCCCGAGGCACGAAGGAGTGCTACCTTATCTGGCTCCGCCACCAAC
TTGGCATCGTTGACAGCTGCCCTGGCCAAGGCCGACAGGAGCCACAAGAACCCTGAGAAC
CGGAAATCCTGGGCCAGT
Protein sequence predicted based on nucleotide sequence
MGDSKVKVAVRIRPMNRRETDLHTKCVVDVDANKVILNPVNTNLSKGDARGQPKVFAYDH CFWSMDESVKEKYAGQDIVFKCLGENILQNAFDGYNACIFAYGQTGSGKSYTMMGTADQP GLIPRLCSGLFERTQKEENEEQSFKVEVSYMEIYNEKVRDLLDPKGSRQTLKVREHSVLG PYVDGLSKLAVTSYKDIESLMSEGNKSRTVAATNMNEESSRSHAVFKITLTHTLYDVKSG TSGEKVGKLSLVDLAGSERATKTGAAGDRLKEGSNINKSLTTLGLVISALADQSAGKNKN KFVPYRDSVLTWLLKDSLGGNSKTAMVATVSPAADNYDETLSTLRYADRAKHIVNHAVVN EDPNARIIRDLREEVEKLREQLTKAEAMKSPELKDRLEESEKLIQEMTVTWEEKLRKTEE IAQERQKQLESLGISLQSSGIKVGDDKCFLVNLNADPALNELLVYYLKEHTLIGSANSQD IQLCGMGILPEHCIIDITSEGQVMLTPQKNTRTFVNGSSVSSPIQLHHGDRILWGNNHFF RLNLPKKKKKAEREDEDQDPSMKNENSSEQLDVDGDSSSEVSSEVNFNYEYAQMEVTMKA LGSNDPMQSILNSLEQQHEEEKRSALERQRLMYEHELEQLRRRLSPEKQNCRSMDRFSFH SPSAQQRLRQWAEEREATLNNSLMRLREQIVKANLLVREANYIAEELDKRTEYKVTLQIP ASSLDANRKRGSLLSEPAIQVRRKGKGKQIWSLEKLDNRLLDMRDLYQEWKECEEDNPVI RSYFKRADPFYDEQENHSLIGVANVFLESLFYDVKLQYAVPIINQKGEVAGRLHVEVMRL SGDVGERIAGGDEVAEVSFEKETQENKLVCMVKILQATGLPQHLSHFVFCKYSFWDQQEP VIVAPEVDTSSSSVSKEPHCMVVFDHCNEFSVNITEDFIEHLSEGALAIEVYGHKINDPR KNPALWDLGIIQAKTRSLRDRWSEVTRKLEFWVQILEQNENGEYCPVEVISAKDVPTGGI FQLRQGQSRRVQVEVKSVQESGTLPLMEECILSVGIGCVKVRPLRAPRTHETFHEEEEDM DSYQDRDLERLRRKWLNALTKRQEYLDQQLQKLVSKRDKTEDDADREAQLLEMRLTLTEE RNAVMVPSAGSGIPGAPAEWTPVPGMETHIPVIFLDLNADDFSSQDNLDDPEAGGWDATL TGEEEEEFFELQIVKQHDGEVKAEASWDSAVHGCPQLSRGTPVDERLFLIVRVTVQLSHP ADMQLVLRKRICVNVHGRQGFAQSLLKKMSHRSSIPGCGVTFEIVSNIPEDAQGVEEREA LARMAANVENPASADSEAYIEKYLRSVLAVENLLTLDRLRQEVAVKEQLTGKGKLSRRSI SSPNVNRLSGSRQDLIPSYSLGSNKGRWESQQDVSQTTVSRGIAPAPALSVSPQNNHSPD PGLSNLAASYLNPVKSFVPQMPKLLKSLFPVRDEKRGKRPSPLAHQPVPRIMVQSASPDI RVTRMEEAQPEMGPDVLVQTMGAPALKICDKPAKVPSPPPVIAVTAVTPAPEAQDGPPSP LSEASSGYFSHSVSTATLSDALGPGLDAAAPPGSMPTAPEAEPEAPISHPPPPTAVPAEE PPGPQQLVSPGRERPDLEAPAPGSPFRVRRVRASELRSFSRMLAGDPGCSPGAEGNAPAP GAGGQALASDSEEADEVPEWLREGEFVTVGAHKTGVVRYVGPADFQEGTWVGVELDLPSG KNDGSIGGKQYFRCNPGYGLLVRPSRVRRATGPVRRRSTGLRLGAPEARRSATLSGSATN LASLTAALAKADRSHKNPENRKSWAS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
