Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGW_NcoI-Eco109I
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745308
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5208nts
Open reading frame: 1 to 5208
ATGGAGCGGTGCAGCCGATGCCATCGCCTCCTCCTGCTCCTACCTCTGGTGCTGGGGCTG
AGCGCGGCCCCAGGCTGGGCTGGTGCACCCCCTGTGGATGTCCTGCGGGCCCTGAGGTTC
CCCTCCCTCCCTGATGGTGTCAGGAGAGCGAAAGGCATCTGTCCAGCTGATGTGGCCTAC
CGAGTGGCACGACCTGCGCAGCTCAGTGCACCCACTCGCCAGCTTTTCCCAGGAGGATTT
CCCAAAGATTTCTCTCTGCTGACTGTTGTCCGGACCCGCCCTGGTCTACAAGCTCCCCTC
CTCACTCTCTACAGTGCCCAGGGTGTCCGACAGCTGGGCCTTGAGCTGGGACGACCTGTC
CGCTTCCTGTATGAAGACCAGACTGGGCGGCCTCAACCTCCCTCTCAGCCAGTCTTCCGA
GGCCTCAGCCTAGCAGATGGCAAGTGGCACCGTGTGGCCGTGGCTGTGAAGGGCCAGTCT
GTCACCCTCATTGTTGACTGCAAGAAGCGAGTCACCAGGCCACTCCCCCGAAGTGCTCGT
CCAGTATTGGACACCCATGGAGTGATCATCTTTGGTGCCCGTATTCTCGATGAAGAAGTC
TTTGAGGGTGATGTCCAGGAGCTGGCCATTGTCCCAGGGGTGCAGGCAGCCTATGAATCA
TGTGAACAGAAGGAGCTGGAATGCGAGGGTGGCCAGAGGGAAAGACCTCAAAACCAACAG
CCTCACAGAGCCCAGAGATCCCCTCAGCAGCAACCATCAAGACTGCACAGGCCTCAAAAT
CAGGAACCCCAGAGCCAGCCCACTGAGTCTCTCTACTATGACTACGAGCCCCCCTATTAT
GATGTGATGACTACGGGGACAACCCCTGATTATCAGGACCCCACCCCAGGTGAAGAGGAA
GAAATCCTGGAGTCGAGCCTCTTGCCACCCCTTGAGGAGGAGCAGACAGATCTGCAGGTC
CCCCCCACAGCCGACAGGTTTCAGGCAGAGGAATATGGGGAGGGTGGTACAGACCCCCCT
GAAGGCCCCTACGATTACACCTATGGCTATGGGGATGATTATCGTGAGGAGACAGAGCTT
GGCCCTGCCCTCTCTGCGGAGACAGCCCACTCAGGAGCCGCTGCCCATGGACCCCGAGGG
CTGAAGGGCGAAAAAGGAGAACCTGCAGTGTTGGAACCTGGTATGCTCGTGGAGGGGCCG
CCTGGCCCAGAAGGCCCTGCGGGATTGATTGGTCCCCCTGGCATCCAGGGGAACCCAGGC
CCAGTTGGAGATCCCGGAGAGCGGGGCCCCCCTGGCCGAGCAGGGCTTCCTGGATCAGAT
GGGGCTCCTGGTCCTCCTGGCACATCTCTCATGCTCCCATTCCGGTTTGGCAGTGGTGGG
GGTGACAAGGGACCTGTGGTGGCGGCCCAGGAGGCTCAGGCGCAGGCCATCCTGCAGCAA
GCGAGACTTGCGCTCCGTGGACCCCCTGGCCCCATGGGATACACAGGGCGCCCTGGGCCC
TTGGGCCAACCTGGGAGCCCCGGCTTGAAAGGCGAGTCTGGAGACTTAGGACCGCAGGGG
CCCAGAGGACCACAGGGCCTCACAGGCCCTCCCGGCAAGGCTGGGCGAAGGGGACGCGCA
GGAGCTGATGGAGCTCGAGGGATGCCCGGAGATCCTGGAGTGAAGGGTGACCGAGGTTTT
GATGGACTCCCCGGGCTCCCTGGCGAAAAGGGCCATAGGGGTGATACTGGTGCCCAAGGC
CTCCCTGGTCCACCCGGTGAGGATGGAGAGAGGGGAGATGACGGGGAGATTGGCCCTCGA
GGCCTGCCTGGAGAGTCTGGACCACGAGGCCTCCTTGGTCCCAAAGGCCCGCCTGGTATT
CCCGGACCCCCTGGCGTCCGAGGCATGGATGGTCCCCAGGGCCCAAAAGGGAGCTTGGGA
CCCCAGGGTGAGCCCGGACCGCCTGGACAACAGGGAACCCCCGGGACCCAGGGTCTTCCC
GGGCCCCAGGGTGCCATCGGCCCTCATGGAGAGAAGGGTCCTCAAGGCAAGCCAGGGCTC
CCCGGCATGCCTGGCTCAGACGGGCCTCCGGGTCACCCCGGGAAGGAAGGTCCCCCTGGA
ACCAAAGGAAACCAGGGTCCCTCTGGACCTCAGGGACCTCTAGGATACCCCGGCCCTCGC
GGCGTCAAGGGCGTGGACGGAATTCGGGGTCTGAAGGGTCATAAGGGTGAGAAAGGGGAG
GATGGCTTCCCTGGGTTCAAAGGCGACATAGGCGTGAAAGGTGACAGGGGCGAAGTTGGA
GTCCCCGGTTCAAGGGGAGAGGATGGTCCTGAGGGGCCAAAGGGACGCACTGGACCGACT
GGTGACCCAGGGCCCCCAGGGCTCATGGGCGAGAAAGGCAAGCTGGGTGTTCCTGGTCTG
CCTGGCTATCCTGGACGTCAGGGACCCAAGGGGTCCCTAGGATTTCCTGGCTTTCCTGGT
GCTAGTGGAGAGAAGGGCGCCCGGGGCCTGTCAGGGAAGTCGGGTCCTCGGGGAGAACGG
GGCCCTACCGGTCCACGAGGTCAGCGGGGACCCCGAGGTGCCACTGGGAAGTCTGGAGCT
AAGGGAACATCTGGTGGTGATGGCCCCCATGGGCCCCCCGGAGAGAGGGGCCTACCTGGA
CCTCAAGGTCCTAACGGGTTTCCTGGACCAAAAGGACCACCCGGCCCCCCTGGGAAGGAT
GGGCTGCCCGGACACCCAGGCCAACGAGGAGAAGTGGGTTTCCAAGGGAAGACCGGCCCA
CCTGGTCCTCCAGGAGTGGTGGGACCTCAGGGAGCAGCCGGAGAAACCGGACCTATGGGG
GAGAGAGGTCACCCAGGACCACCGGGGCCCCCTGGAGAGCAGGGACTGCCTGGGACAGCT
GGAAAAGAAGGAACAAAGGGCGACCCCGGGCCACCTGGGGCCCCAGGAAAGGATGGACCC
GCTGGTCTGCGGGGATTCCCAGGAGAGAGAGGCCTTCCAGGCACTGCTGGTGGACCTGGT
TTGAAGGGGAATGAAGGTCCGTCTGGCCCCCCTGGCCCCGCAGGCTCACCTGGGGAACGA
GGTGCAGCAGGATCAGGGGGACCCATTGGTCCGCCAGGGCGACCAGGCCCTCAGGGTCCA
CCTGGCGCAGCAGGAGAGAAAGGTGTCCCAGGTGAGAAGGGCCCTATTGGCCCGACTGGC
CGAGATGGAGTGCAGGGTCCTGTCGGGCTACCCGGTCCTGCTGGACCTCCCGGTGTGGCT
GGAGAGGACGGAGACAAGGGTGAGGTGGGGGACCCCGGACAGAAGGGCACCAAGGGGAAC
AAGGGTGAACATGGCCCTCCTGGACCCCCTGGACCCATCGGTCCTGTTGGGCAGCCTGGA
GCAGCGGGAGCAGATGGGGAGCCCGGAGCTAGGGGGCCACAGGGACACTTTGGAGCCAAA
GGTGATGAAGGAACAAGAGGATTCAATGGGCCTCCAGGTCCCATTGGCCTACAGGGTTTG
CCAGGCCCCTCTGGCGAGAAGGGAGAAACAGGAGATGTGGGTCCTATGGGACCACCTGGC
CCCCCAGGACCTCGAGGACCAGCCGGACCCAATGGCGCTGATGGCCCACAAGGACCCCCA
GGAGGTGTTGGGAACCTGGGTCCGCCTGGAGAGAAGGGGGAGCCAGGGGAGTCAGGATCT
CCAGGTATCCAGGGCGAGCCAGGTGTCAAGGGTCCAAGAGGGGAACGTGGGGAGAAAGGA
GAGTCGGGGCAGCCAGGAGAGCCTGGGCCACCAGGGCCTAAAGGCCCCACAGGCGATGAT
GGCCCTAAAGGGAACCCTGGACCCGTTGGTTTTCCTGGCGACCCTGGCCCCCCTGGAGAA
GGTGGGCCTCGGGGCCAGGATGGTGCTAAGGGGGACCGAGGCGAGGATGGTGAGCCAGGA
CAGCCTGGATCCCCTGGGCCCACCGGGGAGAATGGACCCCCAGGGCCACTTGGAAAGCGA
GGTCCTGCCGGCTCCCCTGGTTCCGAGGGGCGACAAGGAGGGAAGGGAGCCAAGGGCGAT
CCTGGCGCTATAGGTGCCCCGGGCAAGACCGGACCGGTGGGTCCTGCAGGCCCAGCAGGG
AAACCTGGCCCTGATGGTCTGAGGGGGCTGCCAGGCTCAGTGGGTCAGCAGGGCCGACCT
GGAGCTACAGGCCAGGCTGGGCCCCCAGGTCCTGTGGGACCCCCAGGGCTGCCTGGTCTT
CGGGGCGATGCTGGCGCTAAGGGAGAGAAGGGCCACCCCGGTCTGATTGGACTGATTGGG
CCCCCGGGTGAGCAGGGAGAAAAGGGAGATCGGGGACTTCCTGGTCCTCAGGGCTCTCCC
GGGCAGAAGGGTGAGATGGGTATCCCAGGAGCATCCGGCCCCATTGGGCCTGGAGGTCCC
CCCGGCCTCCCCGGACCTGCTGGCCCCAAAGGTGCCAAAGGAGCCACAGGACCAGGAGGC
CCCAAGGGAGAGAAGGGTGTGCAGGGCCCTCCGGGACACCCGGGTCCCCCAGGCGAGGTG
ATCCAGCCACTGCCCATTCAGATGCCCAAGAAGACTCGGCGCTCGGTGGATGGAAGCCGT
CTGATGCAGGAAGATGAGGCCATACCGACCGGGGGAGCCCCCGGCAGTCCTGGCGGGCTG
GAGGAGATCTTTGGCTCACTCGACTCCCTGCGGGAGGAGATCGAGCAGATGAGGCGGCCA
ACAGGGACTCAGGACAGTCCTGCTCGAACCTGCCAGGACCTGAAGCTGTGCCACCCAGAG
CTTCCCGATGGCGAGTACTGGGTCGACCCCAACCAGGGATGTGCTCGGGATGCCTTCCGA
GTTTTCTGCAACTTCACAGCAGGGGGTGAGACATGTGTGACGCCTAGGGATGACGTCACG
CAGTTCTCTTACGTGGACTCAGAGGGCTCCCCAGTGGGTGTGGTCCAGCTCACCTTCCTG
CGGCTGCTGAGTGTCTCAGCCCACCAGGACGTCTCCTACCCCTGCTCCGGCGCAGCCCGT
GACGGTCCCCTGAGACTCCGTGGGGCCAATGAGGATGAGCTGAGCCCCGAGACTAGCCCC
TATGTCAAAGAATTCAGAGATGGCTGCCAGACACAGCAAGGCCGGACGGTGCTGGAGGTG
CGAACGCCTGTGCTGGAGCAGCTGCCAGTGCTGGATGCCTCTTTCTCAGACCTGGGCGCC
CCACCGAGGCGGGGAGGGGTGCTGCTGGGGCCTGTCTGCTTCATGGGA
Protein sequence predicted based on nucleotide sequence
MERCSRCHRLLLLLPLVLGLSAAPGWAGAPPVDVLRALRFPSLPDGVRRAKGICPADVAY RVARPAQLSAPTRQLFPGGFPKDFSLLTVVRTRPGLQAPLLTLYSAQGVRQLGLELGRPV RFLYEDQTGRPQPPSQPVFRGLSLADGKWHRVAVAVKGQSVTLIVDCKKRVTRPLPRSAR PVLDTHGVIIFGARILDEEVFEGDVQELAIVPGVQAAYESCEQKELECEGGQRERPQNQQ PHRAQRSPQQQPSRLHRPQNQEPQSQPTESLYYDYEPPYYDVMTTGTTPDYQDPTPGEEE EILESSLLPPLEEEQTDLQVPPTADRFQAEEYGEGGTDPPEGPYDYTYGYGDDYREETEL GPALSAETAHSGAAAHGPRGLKGEKGEPAVLEPGMLVEGPPGPEGPAGLIGPPGIQGNPG PVGDPGERGPPGRAGLPGSDGAPGPPGTSLMLPFRFGSGGGDKGPVVAAQEAQAQAILQQ ARLALRGPPGPMGYTGRPGPLGQPGSPGLKGESGDLGPQGPRGPQGLTGPPGKAGRRGRA GADGARGMPGDPGVKGDRGFDGLPGLPGEKGHRGDTGAQGLPGPPGEDGERGDDGEIGPR GLPGESGPRGLLGPKGPPGIPGPPGVRGMDGPQGPKGSLGPQGEPGPPGQQGTPGTQGLP GPQGAIGPHGEKGPQGKPGLPGMPGSDGPPGHPGKEGPPGTKGNQGPSGPQGPLGYPGPR GVKGVDGIRGLKGHKGEKGEDGFPGFKGDIGVKGDRGEVGVPGSRGEDGPEGPKGRTGPT GDPGPPGLMGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGASGEKGARGLSGKSGPRGER GPTGPRGQRGPRGATGKSGAKGTSGGDGPHGPPGERGLPGPQGPNGFPGPKGPPGPPGKD GLPGHPGQRGEVGFQGKTGPPGPPGVVGPQGAAGETGPMGERGHPGPPGPPGEQGLPGTA GKEGTKGDPGPPGAPGKDGPAGLRGFPGERGLPGTAGGPGLKGNEGPSGPPGPAGSPGER GAAGSGGPIGPPGRPGPQGPPGAAGEKGVPGEKGPIGPTGRDGVQGPVGLPGPAGPPGVA GEDGDKGEVGDPGQKGTKGNKGEHGPPGPPGPIGPVGQPGAAGADGEPGARGPQGHFGAK GDEGTRGFNGPPGPIGLQGLPGPSGEKGETGDVGPMGPPGPPGPRGPAGPNGADGPQGPP GGVGNLGPPGEKGEPGESGSPGIQGEPGVKGPRGERGEKGESGQPGEPGPPGPKGPTGDD GPKGNPGPVGFPGDPGPPGEGGPRGQDGAKGDRGEDGEPGQPGSPGPTGENGPPGPLGKR GPAGSPGSEGRQGGKGAKGDPGAIGAPGKTGPVGPAGPAGKPGPDGLRGLPGSVGQQGRP GATGQAGPPGPVGPPGLPGLRGDAGAKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSP GQKGEMGIPGASGPIGPGGPPGLPGPAGPKGAKGATGPGGPKGEKGVQGPPGHPGPPGEV IQPLPIQMPKKTRRSVDGSRLMQEDEAIPTGGAPGSPGGLEEIFGSLDSLREEIEQMRRP TGTQDSPARTCQDLKLCHPELPDGEYWVDPNQGCARDAFRVFCNFTAGGETCVTPRDDVT QFSYVDSEGSPVGVVQLTFLRLLSVSAHQDVSYPCSGAARDGPLRLRGANEDELSPETSP YVKEFRDGCQTQQGRTVLEVRTPVLEQLPVLDASFSDLGAPPRRGGVLLGPVCFMG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGW_NcoI-Eco109I
Synonyms:
pGW-NcoEco
Description:
In-fusion cloning vector with NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette; kanamycin resistance in bacteria; recombinational cloning
Comments:
NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette. Made for use with In-Fusion cloning
Size (bp):
4008
Parent Vector:
None
Properties:
''
Author Name:
Joshua LaBaer
Andreas Rolf
Andreas Rolf
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3332
4005
death cassette
ccdB
ccdB death cassette - removed in plasmids containing an insert
1779
2084
primer
M13 forward
M13 forward sequencing primer
536
553
primer
M13 reverse
M13 reverse sequencing primer
2269
2289
recombination site
attL1
attL1 recombinational cloning site
644
668
recombination site
attL2
attL2 recombinational cloning site
2133
2157
selectable marker
KanR
kanamycin resistance
2402
3208
selectable marker
CmR
Chloramphenicol resistance - removed in plasmids containing an insert
778
1434
