Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745373
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5679nts
Open reading frame: 1 to 5679
ATGATGGACTTGGTGTTGGAAGAGGACGTCACCGTCCCTGGGACGCTCAGCGGCTGCAGT
GGCCTGGTTCCTAGTGTACCAGATGACCTGGATGGCATCAACCCCAATGCTGGGTTGGGA
AATGGTCTGCTCCCAAATGTGTCAGAAGAAACAGTGTCTCCCACCAGAGCACGGAACATG
AAGGACTTTGAAAATCAAATCACTGAGTTGAAGAAAGAAAACTTTAACCTGAAGCTCCGC
ATCTATTTCCTTGAGGAAAGAATGCAACAGGAATTTCATGGCCCCACTGAACATATCTAC
AAAACTAACATTGAGCTCAAGGTGGAAGTAGAAAGTCTGAAGCGGGAACTGCAGGAGAGA
GAGCAGCTGCTCATCAAAGCCTCCAAAGCAGTTGAGAGCTTAGCTGAAGCAGGCGGCTCT
GAAATCCAGCGGGTGAAAGAAGATGCTCGAAAGAAGGTGCAGCAAGTGGAAGATCTCCTA
ACTAAAAGAATACTCCTTTTGGAAAAGGATGTGACCGCCGCCCAGGCAGAACTGGAAAAG
GCCTTTGCAGGGACAGAGACGGAGAAGGCTCTTCGGTTGCGTCTGGAAAGCAAGCTTTCA
GAGATGAAGAAGATGCACGAGGGGGACTTGGCGATGGCTCTGGTCCTGGATGAGAAAGAC
AGACTGATTGAGGAGTTGAAGCTGTCTTTGAAGAGCAAAGAAGCTTTAATTCAGTGCCTT
AAAGAGGAGAAATCTCAGATGGCATGTCCTGATGAGAATGTGTCATCTGGAGAGCTCCGA
GGACTTTGTGCTGCTCCAAGGGAAGAAAAGGAGAGAGAAACTGAGGCTGCACAAATGGAG
CATCAGAAGGAGAGAAACAGCTTTGAAGAGAGGATCCAGGCACTTGAAGAGGACCTGAGA
GAGAAGGAAAGAGAAATTGCTACAGAGAAGAAAAATAGCCTGAAGAGGGATAAAGCCATC
CAGGGTTTAACCATGGCATTGAAATCAAAGGAAAAAAAGGTTGAAGAACTGAACTCTGAA
ATTGAAAAGCTGAGTGCTGCCTTTGCTAAAGCCAGGGAGGCCCTACAGAAAGCACAGACC
CAGGAATTTCAGGGGTCTGAAGACTATGAGACTGCTCTATCAGGAAAGGAAGCCCTTTCG
GCTGCGCTGCGCTCACAAAACCTCACCAAGAGTACAGAGAACCACAGGCTGCGTAGAAGC
ATTAAGAAGATCACCCAGGAGCTGAGTGACTTGCAGCAGGAGAGGGAGAGACTGGAGAAG
GACCTGGAGGAAGCCCATCGAGAGAAGAGTAAAGGAGACTGCACCATCCGTGATCTGAGG
AATGAAGTGGAAAAATTACGCAATGAAGTGAATGAGAGAGAGAAAGCAATGGAAAACCGG
TACAAGAGTCTTCTGAGTGAAAGCAATAAAAAATTGCACAATCAAGAGCAAGTGATCAAA
CATCTAACAGAAAGCACCAATCAGAAGGACGTGCTGCTGCAGAAATTCAATGAAAAAGAT
TTGGAAGTAATACAGCAGAACTGCTATTTAATGGCTGCAGAGGATCTTGAGCTCAGGAGT
GAAGGCTTAATAACAGAAAAGTGCTCCTCACAACAGCCACCAGGCAGTAAAACCATCTTC
TCTAAGGAAAAGAAACAATCATCAGACTATGAAGAGCTGATTCAGGTCTTAAAGAAAGAG
CAGGACATCTATACCCATCTGGTCAAATCTCTGCAGGAATCAGACAGTATCAACAACCTG
CAGGCTGAGTTAAACAAGATTTTTGCCCTGCGGAAGCAACTGGAGCAGGATGTGCTTTCA
TATCAGAATTTGCGGAAGACCTTGGAGGAGCAGATCAGCGAAATTCGGAGGCGGGAAGAA
GAATCATTTTCCCTTTATAGTGACCAAACATCCTATCTAAGCATTTGCCTGGAAGAAAAC
AATCGGTTTCAAGTGGAACATTTTTCTCAAGAGGAACTGAAGAAAAAGGTCAGTGACCTT
ATACAGCTAGTGAAGGAGCTGTATACAGACAACCAGCACCTGAAGAAAACCATTTTTGAT
CTCTCCTGCATGGGTTTCCAGGGAAATGGGTTTCCAGATAGACTTGCGTCTACAGAACAA
ACAGAGCTTCTGGCTAGCAAGGAGGACGAGGACACGATCAAAATTGGGGAGGATGACGAG
ATTAATTTCCTGAGTGACCAGCATTTGCAGCAGAGCAATGAGATTATGAAAGACCTTTCC
AAAGGAGGCTGCAAAAATGGATACTTAAGGCACACGGAGTCTAAGATTTCAGATTGTGAT
GGGGCCCACGCACCTGGCTGCCTAGAAGAAGGTGCATTCATAAACCTGCTTGCCCCTTTG
TTCAATGAGAAGGCCACATTATTACTGGAATCCAGGCCAGACCTTCTCAAAGTGGTACGG
GAACTGCTTCTCGGACAACTATTCTTGACAGAGCAGGAAGTTTCTGGAGAACACCTTGAT
GGTAAAACTGAGAAGACACCTAAGCAAAAAGGTGAACTTGTACATTTTGTCCAAACCAAC
TCATTTTCCAAGCCACATGATGAACTGAAGTTGTCTTGTGAGGCCCAGCTAGTAAAGGCA
GGCGAAGTGCCCAAGGTAGGACTGAAAGATGCCTCAGTGCAGACTGTGGCCACGGAGGGC
GACCTGCTGAGATTCAAGCATGAAGCAACAAGAGAGGCTTGGGAAGAGAAACCGATCAAC
ACTGCACTCAGTGCAGAGCATCGGCCAGAGAACCTGCACGGGGTGCCTGGGTGGCAGGCT
GCCCTCCTGTCCCTCCCTGGTATTACCAACAGAGAGGCTAAGAAGTCCCGCTTGCCAATC
CTAATAAAACCATCCCGGTCATTAGGAAATATGTATCGTCTCCCTGCCACCCAGGAGGTG
GTGACGCAGCTGCAGAGCCAGATCTTGGAGCTCCAGGGGGAGCTGAAGGAGTTTAAAACT
TGTAATAAGCAACTTCACCAAAAGTTAATTCTGGCTGAAGCAGTGATGGAGGGGAGGCCA
ACGCCCGACAAAACGTTGCTGAATGCTCAGCCCCCCGTGGGAGCAGCCTACCAGGACAGC
CCAGGAGAGCAGAAAGGAATTAAAACCACATCTTCTGTCTGGAGAGACAAGGAAATGGAC
AGTGATCAGCAAAGAAGCTACGAGATTGACTCTGAGATTTGCCCACCTGATGACCTTGCT
AGCTTGCCATCATGCAAAGAAAATCCTGAAGATGTTCTGAGCCCAACTTCAGTAGCTACT
TACCTGAGTTCCAAGAGTCAGCCTTCTGCTAAAGTCAGTGTGATGGGGACTGATCAGTCA
GAGAGCATTAATACCTCAAATGAGACAGAGTACTTAAAACAGAAAATCCATGACTTGGAA
ACTGAGCTGGAAGGCTACCAGAATTTCATATTTCAGCTTCAAAAGCACTCCCAGTGCAGT
GAGGCCATAATTACAGTTTTGTGTGGCACAGAAGGGGCCCAGGATGGCTTGAGTAAGCCC
AAGAATGGTTCTGATGGGGAAGAAATGACCTTTTCAAGTTTGCACCAAGTGCGATACGTG
AAACACGTGAAAATCCTCGGTCCGCTGGCCCCAGAGATGATTGACAGCAGGGTGCTGGAG
AACCTCAAACAGCAGCTGGAGGAACAGGAATACAAGCTGCAGAAGGAGCAGAATTTGAAC
ATGCAACTTTTCAGTGAGATCCATAATCTGCAGAATAAGTTCAGAGATCTCTCACCTCCC
AGATACGATTCATTAGTTCAGTCCCAAGCCAGGGAGCTCTCCCTTCAACGGCAGCAGATT
AAGGATGGCCATGGCATCTGTGTCATCTCCCGTCAACACATGAACACCATGATTAAGGCA
TTTGAGGAGTTGCTGCAGGCCAGTGATGTGGATTACTGTGTGGCCGAGGGTTTCCAGGAA
CAGCTCAATCAATGTGCTGAGCTGCTGGAGAAATTGGAAAAGCTATTTCTCAACGGAAAA
TCAGTTGGAGTGGAAATGAACACCCAGAATGAACTGATGGAGAGGATTGAGGAAGACAAC
TTAACCTACCAACATCTTCTGCCTGAATCTCCTGAGCCTTCAGCCTCTCATGCGCTCTCT
GATTATGAAACATCTGAAAAGTCCTTCTTCTCACGGGACCAGAAGCAAGATAATGAGACA
GAGAAGACTTCAGTTATGGTGAACAGTTTTTCTCAAGACCTGCTAATGGAACACATACAG
GAAATTCGGACTTTGAGAAAGCGTTTAGAAGAATCTATCAAGACAAATGAGAAGCTACGG
AAACAGTTGGAACGGCAAGGATCTGAATTTGTTCAAGGTTCTACAAGCATTTTTGCTTCT
GGTTCAGAGCTTCATAGTTCTCTAACATCAGAAATTCATTTCTTGAGGAAGCAGAACCAG
GCCCTCAATGCAATGCTCATTAAAGGATCCAGAGATAAACAGAAGGAGAATGACAAATTA
CGAGAGTCCCTCTCCAGGAAGACCGTGAGCCTGGAGCACCTTCAGCGGGAGTATGCCAGC
GTGAAGGAAGAAAATGAAAGGCTGCAGAAAGAAGGCAGCGAGAAGGAGAGACACAACCAG
CAGCTGATCCAGGAGGTCCGCTGCAGTGGCCAGGAGCTGAGCAGGGTGCAGGAGGAGGTG
AAGTTGAGGCAGCAGCTGCTCTCACAGAATGACAAGCTATTGCAGTCTCTCCGAGTGGAG
CTGAAGGCGTATGAGAAGCTGGATGAAGAGCACAGGAGGCTGAGAGAGGCGTCGGGAGAA
GGCTGGAAGGGGCAGGATCCTTTCAGGGACCTGCACAGTCTCCTGATGGAGATCCAGGCT
CTGCGCTTGCAACTAGAAAGAAGCATCGAAACCAGCAGCACTCTGCAGAGCAGGCTCAAG
GAACAGCTGGCAAGGGGCGCAGAGAAGGCACAGGAAGGAGCCCTGACTCTGGCTGTCCAA
GCCGTGTCTATCCCTGAGGTGCCCCTTCAGCCTGACAAACACGATGGTGACAAATATCCC
ATGGAAAGTGATAATTCATTTGATCTGTTTGATTCCTCCCAGGCAGTGACACCAAAATCA
GTTTCAGAGACTCCTCCCCTCTCTGGGAATGACACGGACTCCCTCTCCTGCGACAGTGGC
AGTTCGGCAACTAGCACTCCGTGTGTGTCCCGACTGGTCACTGGCCACCACCTGTGGGCC
AGTAAGAATGGCCGCCATGTCCTGGGCCTGATTGAGGACTATGAGGCCCTGCTCAAACAG
ATCAGCCAGGGACAGAGGCTCCTTGCTGAAATGGACATTCAAACCCAAGAGGCTCCCAGC
TCCACAAGTCAAGAGCTGGGAACAAAGGGTCCACACCCAGCACCACTGAGCAAGTTTGTG
AGCAGTGTGAGCACGGCCAAGCTGACCCTGGAAGAGGCCTACAGACGGCTGAAGCTTCTC
TGGAGAGTCTCACTGCCCGAGGATGGCCAGTGCCCCCTTCACTGTGAGCAGATTGGAGAA
ATGAAGGCAGAGGTCACCAAACTACATAAAAAACTGTTTGAACAAGAAAAGAAGTTGCAA
AACACCATGAAGCTTTTGCAGCTGAGCAAGCGCCAGGAAAAAGTCATCTTTGATCAATTG
GTCGTAACCCACAAAATCCTTCGGAAGGCCAGAGGAAACCTGGAGCTTAGGCCTGGGGGA
GCCCATCCAGGAACATGCAGTCCCAGCAGGCCAGGCTCC
Protein sequence predicted based on nucleotide sequence
MMDLVLEEDVTVPGTLSGCSGLVPSVPDDLDGINPNAGLGNGLLPNVSEETVSPTRARNM KDFENQITELKKENFNLKLRIYFLEERMQQEFHGPTEHIYKTNIELKVEVESLKRELQER EQLLIKASKAVESLAEAGGSEIQRVKEDARKKVQQVEDLLTKRILLLEKDVTAAQAELEK AFAGTETEKALRLRLESKLSEMKKMHEGDLAMALVLDEKDRLIEELKLSLKSKEALIQCL KEEKSQMACPDENVSSGELRGLCAAPREEKERETEAAQMEHQKERNSFEERIQALEEDLR EKEREIATEKKNSLKRDKAIQGLTMALKSKEKKVEELNSEIEKLSAAFAKAREALQKAQT QEFQGSEDYETALSGKEALSAALRSQNLTKSTENHRLRRSIKKITQELSDLQQERERLEK DLEEAHREKSKGDCTIRDLRNEVEKLRNEVNEREKAMENRYKSLLSESNKKLHNQEQVIK HLTESTNQKDVLLQKFNEKDLEVIQQNCYLMAAEDLELRSEGLITEKCSSQQPPGSKTIF SKEKKQSSDYEELIQVLKKEQDIYTHLVKSLQESDSINNLQAELNKIFALRKQLEQDVLS YQNLRKTLEEQISEIRRREEESFSLYSDQTSYLSICLEENNRFQVEHFSQEELKKKVSDL IQLVKELYTDNQHLKKTIFDLSCMGFQGNGFPDRLASTEQTELLASKEDEDTIKIGEDDE INFLSDQHLQQSNEIMKDLSKGGCKNGYLRHTESKISDCDGAHAPGCLEEGAFINLLAPL FNEKATLLLESRPDLLKVVRELLLGQLFLTEQEVSGEHLDGKTEKTPKQKGELVHFVQTN SFSKPHDELKLSCEAQLVKAGEVPKVGLKDASVQTVATEGDLLRFKHEATREAWEEKPIN TALSAEHRPENLHGVPGWQAALLSLPGITNREAKKSRLPILIKPSRSLGNMYRLPATQEV VTQLQSQILELQGELKEFKTCNKQLHQKLILAEAVMEGRPTPDKTLLNAQPPVGAAYQDS PGEQKGIKTTSSVWRDKEMDSDQQRSYEIDSEICPPDDLASLPSCKENPEDVLSPTSVAT YLSSKSQPSAKVSVMGTDQSESINTSNETEYLKQKIHDLETELEGYQNFIFQLQKHSQCS EAIITVLCGTEGAQDGLSKPKNGSDGEEMTFSSLHQVRYVKHVKILGPLAPEMIDSRVLE NLKQQLEEQEYKLQKEQNLNMQLFSEIHNLQNKFRDLSPPRYDSLVQSQARELSLQRQQI KDGHGICVISRQHMNTMIKAFEELLQASDVDYCVAEGFQEQLNQCAELLEKLEKLFLNGK SVGVEMNTQNELMERIEEDNLTYQHLLPESPEPSASHALSDYETSEKSFFSRDQKQDNET EKTSVMVNSFSQDLLMEHIQEIRTLRKRLEESIKTNEKLRKQLERQGSEFVQGSTSIFAS GSELHSSLTSEIHFLRKQNQALNAMLIKGSRDKQKENDKLRESLSRKTVSLEHLQREYAS VKEENERLQKEGSEKERHNQQLIQEVRCSGQELSRVQEEVKLRQQLLSQNDKLLQSLRVE LKAYEKLDEEHRRLREASGEGWKGQDPFRDLHSLLMEIQALRLQLERSIETSSTLQSRLK EQLARGAEKAQEGALTLAVQAVSIPEVPLQPDKHDGDKYPMESDNSFDLFDSSQAVTPKS VSETPPLSGNDTDSLSCDSGSSATSTPCVSRLVTGHHLWASKNGRHVLGLIEDYEALLKQ ISQGQRLLAEMDIQTQEAPSSTSQELGTKGPHPAPLSKFVSSVSTAKLTLEEAYRRLKLL WRVSLPEDGQCPLHCEQIGEMKAEVTKLHKKLFEQEKKLQNTMKLLQLSKRQEKVIFDQL VVTHKILRKARGNLELRPGGAHPGTCSPSRPGS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
