Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pGW_NcoI-Eco109I
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745607
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4710nts
Open reading frame: 1 to 4710
ATGGAACCGGGATGTGACGAGTTCCTTCCGCCACCGGAGTGCCCGGTTTTTGAGCCTAGC
TGGGCTGAATTCCAAGACCCGCTTGGCTACATTGCGAAAATAAGGCCCATAGCAGAGAAA
TCTGGGATCTGCAAAATCCGCCCACCCGCGGATTGGCAGCCTCCTTTTGCAGTAGAAGTT
GACAATTTCAGATTTACTCCTCGGGTCCAAAGGCTGAATGAACTGGAGGCCCAAACTAGA
GTGAAATTGAACTATTTGGATCAGATTGCAAAATTCTGGGAAATTCAAGGATCCTCTTTA
AAGATTCCCAATGTGGAGCGGAAGATCTTGGACCTCTACAGCCTTAGTAAGATTGTGATT
GAGGAAGGTGGCTATGAAGCCATCTGCAAGGATCGGAGGTGGGCTCGAGTTGCCCAGCGT
CTCCACTACCCACCAGGCAAAAACATTGGCTCCCTGCTACGATCACATTACGAACGGATT
ATTTACCCCTATGAAATGTTTCAGTCTGGAGCCAACCATGTGCAATGTAACACACACCCG
TTTGACAATGAGGTAAAAGATAAGGAATACAAGCCCCACAGCATCCCCCTTAGACAGTCT
GTGCAGCCTTCAAAGTTCAGCAGCTACAGTCGGCGGGCCAAAAGGCTACAGCCTGATCCA
GAGCCTACAGAGGAGGACATTGAGAAGCATCCAGAGCTAAAGAAGTTACAGATATATGGG
CCAGGCCCCAAAATGATGGGCTTGGGCCTTATGGCTAAGGATAAGGATAAGACTGTGCAT
AAGAAAGTCACATGCCCCCCCACTGTTACGGTGAAGGATGAGCAAAGTGGAGGTGGGAAC
GTGTCATCAACATTGCTCAAGCAGCACTTGAGCCTAGAGCCCTGCACTAAGACAACCATG
CAACTCCGAAAGAATCACAGCAGTGCCCAGTTTATTGACTCCTATATTTGCCAAGTATGC
TCCCGAGGCGATGAAGATGATAAGCTTCTTTTCTGTGATGGCTGTGATGACAATTACCAC
ATCTTCTGCTTGTTACCACCCCTTCCTGAAATCCCCAGAGGCATCTGGAGGTGCCCAAAA
TGTATCTTGGCGGAGTGTAAACAGCCTCCTGAAGCTTTTGGATTTGAACAGGCTACCCAG
GAGTACAGTTTGCAGAGTTTTGGTGAAATGGCCGATTCTTTCAAGTCCGACTACTTCAAC
ATGCCTGTACATATGGTGCCCACAGAACTTGTAGAGAAGGAATTCTGGAGGCTGGTGAGC
AGCATCGAGGAAGACGTGACAGTTGAATATGGAGCCGATATTCATTCCAAAGAATTTGGC
AGTGGCTTTCCTGTCAGCAATAGTAAACAAAACTTGTCTCCTGAGGAGAAGAGGCAAAGT
CTGACTGTGCTGACCAGGCTCATCTCAAGCTTCTGGGCCCAAGCCGTCCTCCCACCTTGG
CCCCCCAAAGTGCTGGGATTACAGGAGTATGCGACCAGTGGTTGGAACCTGAATGTTATG
CCAGTGCTAGATCAGTCTGTGCTCTGTCACATCAATGCAGACATTTCAGGCATGAAGGTG
CCCTGGCTGTACGTGGGAATGGTTTTCTCCGCATTTTGTTGGCATATTGAGGATCACTGG
AGTTACTCTATTAACTATCTGCATTGGGGTGAGCCGAAGACCTGGTATGGTGTACCCTCC
CTGGCAGCAGAGCATTTGGAGGAGGTGATGAAGATGCTGACACCTGAGCTGTTTGATTCC
CAGCCTGATCTCCTACACCAGCTTGTCACACTCATGAATCCCAACACGTTGATGTCCCAT
GGCGTGCCAGTTGTCCGCACAAACCAGTGTGCAGGGGAGTTTGTCATCACTTTTCCTCGT
GCTTACCACAGCGGTTTTAACCAAGGCTACAATTTTGCTGAGGCTGTCAACTTTTGTACT
GCTGACTGGCTACCTGCTGGACGACAGTGCATTGAACACTACCGCCGGCTCAGGCGCTAT
TGTGTCTTCTCTCACGAGGAGCTTATCTGCAAGATGGCCGCCTTCCCAGAGACGTTGGAT
CTCAATCTGGCAGTAGCTGTGCACAAGGAGATGTTCATTATGGTTCAGGAGGAGCGACGT
CTACGAAAGGCCCTTTTGGAGAAGGGAGTCACGGAGGCTGAGCGAGAGGCTTTTGAGCTG
CTCCCCGATGATGAACGCCAGTGCATTAAGTGCAAGACCACTTGCTTCTTGTCAGCCCTG
GCCTGCTACGACTGCCCAGATGGCCTTGTATGCCTGTCCCACATCAATGACCTCTGCAAG
TGCTCTAGTAGCCGACAGTACCTCCGGTATCGGTACACCTTGGATGAGCTCCCCACCATG
CTGCATAAACTGAAGATTCGGGCTGAGTCTTTTGACACCTGGGCCAACAAAGTGCGAGTG
GCCTTGGAGGTGGAGGATGGCCGTAAACGCAGCTTTGAAGAGCTAAGGGCCCTGGAGTCT
GAGGCTCGTGAGAGGAGGTTTCCCAATAGTGAGCTGCTTCAGCGACTGAAGAACTGCCTG
AGTGAGGTGGAGGCTTGTATCGCTCAAGTCCTCGGGCTGGTCAGTGGTCAGGTCGCCAGG
ATGGACACTCCCCAGCTCACATTGACCGAACTCCGGGTCCTTCTTGAGCAGATGGGCAGC
CTGCCCTGTGCCATGCATCAGATTGGCGATGTCAAGGATGTCCTGGAACAGGTGGAGGCC
TACCAAGCTGAGGCTCGTGAGGCTCTGGCCACACTGCCCTCCAGTCCAGGGCTATTGCGG
TCCCTGTTGGAGAGGGGGCAGCAGCTGGGTGTAGAGGTGCCTGAAGCCCATCAGCTTCAG
CAGCAGGTCGAGCAGGCGCAATGGCTAGATGAAGTGAAGCAGGCCCTCGCCCCTTCTGCT
CACAGAGGCTCTCTGGTCATCATGCAGGGGCTTTTGGTTATGGGAGCCAAGATAGCCTCC
AGCCCTTCTGTGGACAAGGCCCGGGCTGAGCTGCAAGAACTACTGACCATTGCAGAGCGC
TGGGAAGAAAAGGCTCATTTCTGCCTGGAGGCCAGGCAGAAGCATCCACCAGCCACATTG
GAAGCCATAATTCGTGAGACAGAAAACATCCCTGTTCATCTGCCTAACATCCAGGCTCTC
AAAGAAGCTCTGACTAAGGCACAAGCTTGGATTGCTGATGTGGATGAGATCCAAAATGGT
GACCACTACCCCTGTCTAGATGACTTGGAGGGCCTTGTGGCTGTCGGCCGGGACCTGCCT
GTGGGGCTCGAAGAGCTGAGACAGCTAGAGCTCCAGGTATTGACAGCACATTCCTGGAGA
GAGAAGGCCTCCAAGACCTTTCTCAAGAAGAATTCTTGCTACACACTGCTTGAGGTGCTG
TGCCCGTGTGCAGACGCTGGATCAGACAGCACCAAGCGTAGCCGGTGGATGGAGAAGGCG
CTGGGGTTGTACCAGTGTGACACAGAGCTGCTGGGGCTGTCTGCACAGGACCTCAGGGAC
CCAGGCTCTGTGATTGTGGCCTTCAAGGAAGGGGAACAGAAGGAGAAGGAGGGTATCCTG
CAGCTGCGGCGCACCAACTCAGCCAAGCCCAGTCCACTGGCACCATCCCTCATGGCCTCT
TCTCCGACTTCTATCTGTGTGTGCGGGCAAGTGCCAGCTGGGGTTGGAGTTCTGCAGTGT
GACCTGTGTCAGGACTGGTTCCATGGGCAGTGTGTGTCAGTGCCCCATCTCCTCACCTCT
CCAAAGCCCAGTCTCACTTCATCTCCACTGCTAGCCTGGTGGGAATGGGACACAAAATTC
CTGTGTCCACTGTGTATGCGCTCACGACGGCCACGCCTAGAGACAATCCTAGCCTTGCTG
GTGGCCCTGCAGAGACTGCCCGTGCGGCTGCCTGAGGGTGAGGCCCTGCAGTGTCTCACA
GAGAGGGCCATTGGCTGGCAAGACCGTGCAAGGAAGGCCCTGGCCTCTGAAGATGTGACT
GCTCTGTTGCGACAGCTGGCTGAGCTTCGGCAGCAGCTACAGGCCAAACCCAGACCAGAG
GAGGCCTCAGTCTACACTTCAGCCACTGCCTGTGACCCTATAAGGGAAGGAAGTGGCAAC
AATATTTCTAAGGTCCAAGGGCTGCTCGAGAATGGAGACAGTGTGACCAGTCCTGAGAAC
ATGGCTCCAGGAAAGGGCTCTGACCTGGAACTACTGTCATCACTGTTGCCGCAGTTGACT
GGGCCTGTGTTGGAGCTGCCTGAGGCAATCCGGGCTCCCCTGGAGGAGCTGATGATGGAA
GGGGACCTGCTTGAGGTGACCCTGGATGAGAACCACAGCATCTGGCAGCTGCTCCAGGCT
GGACAGCCTCCAGACCTCGACCGAATTCGCACACTTTTAGAGCTGGAAAAATTTGAACAT
CAAGGGAGTCGGACAAGGAGCAGGGCCCTCGAGAGGCGAAGGCGGCGGCAGAAGGTTGAT
CAGGGTAGAAACGTTGAGAATCTTGTTCAACAGGAGCTTCAGTCAAAAAGGGCTCGGAGC
TCAGGGATTATGTCTCAGGTGGGCCGAGAAGAAGAACACTATCAGGAGAAAGCAGACCGT
GAAAATATGTTCCTGACACCTTCCACAGACCACTCTCCTTTCTTGAAAGGAAACCAAAAT
AGCTTACAACACAAGGATTCAGGCTCTTCAGCTGCTTGTCCTTCTTTGATGCCTTTGCTA
CAACTCTCCTACTCTGATGAGCAACAGTTG
Protein sequence predicted based on nucleotide sequence
MEPGCDEFLPPPECPVFEPSWAEFQDPLGYIAKIRPIAEKSGICKIRPPADWQPPFAVEV DNFRFTPRVQRLNELEAQTRVKLNYLDQIAKFWEIQGSSLKIPNVERKILDLYSLSKIVI EEGGYEAICKDRRWARVAQRLHYPPGKNIGSLLRSHYERIIYPYEMFQSGANHVQCNTHP FDNEVKDKEYKPHSIPLRQSVQPSKFSSYSRRAKRLQPDPEPTEEDIEKHPELKKLQIYG PGPKMMGLGLMAKDKDKTVHKKVTCPPTVTVKDEQSGGGNVSSTLLKQHLSLEPCTKTTM QLRKNHSSAQFIDSYICQVCSRGDEDDKLLFCDGCDDNYHIFCLLPPLPEIPRGIWRCPK CILAECKQPPEAFGFEQATQEYSLQSFGEMADSFKSDYFNMPVHMVPTELVEKEFWRLVS SIEEDVTVEYGADIHSKEFGSGFPVSNSKQNLSPEEKRQSLTVLTRLISSFWAQAVLPPW PPKVLGLQEYATSGWNLNVMPVLDQSVLCHINADISGMKVPWLYVGMVFSAFCWHIEDHW SYSINYLHWGEPKTWYGVPSLAAEHLEEVMKMLTPELFDSQPDLLHQLVTLMNPNTLMSH GVPVVRTNQCAGEFVITFPRAYHSGFNQGYNFAEAVNFCTADWLPAGRQCIEHYRRLRRY CVFSHEELICKMAAFPETLDLNLAVAVHKEMFIMVQEERRLRKALLEKGVTEAEREAFEL LPDDERQCIKCKTTCFLSALACYDCPDGLVCLSHINDLCKCSSSRQYLRYRYTLDELPTM LHKLKIRAESFDTWANKVRVALEVEDGRKRSFEELRALESEARERRFPNSELLQRLKNCL SEVEACIAQVLGLVSGQVARMDTPQLTLTELRVLLEQMGSLPCAMHQIGDVKDVLEQVEA YQAEAREALATLPSSPGLLRSLLERGQQLGVEVPEAHQLQQQVEQAQWLDEVKQALAPSA HRGSLVIMQGLLVMGAKIASSPSVDKARAELQELLTIAERWEEKAHFCLEARQKHPPATL EAIIRETENIPVHLPNIQALKEALTKAQAWIADVDEIQNGDHYPCLDDLEGLVAVGRDLP VGLEELRQLELQVLTAHSWREKASKTFLKKNSCYTLLEVLCPCADAGSDSTKRSRWMEKA LGLYQCDTELLGLSAQDLRDPGSVIVAFKEGEQKEKEGILQLRRTNSAKPSPLAPSLMAS SPTSICVCGQVPAGVGVLQCDLCQDWFHGQCVSVPHLLTSPKPSLTSSPLLAWWEWDTKF LCPLCMRSRRPRLETILALLVALQRLPVRLPEGEALQCLTERAIGWQDRARKALASEDVT ALLRQLAELRQQLQAKPRPEEASVYTSATACDPIREGSGNNISKVQGLLENGDSVTSPEN MAPGKGSDLELLSSLLPQLTGPVLELPEAIRAPLEELMMEGDLLEVTLDENHSIWQLLQA GQPPDLDRIRTLLELEKFEHQGSRTRSRALERRRRRQKVDQGRNVENLVQQELQSKRARS SGIMSQVGREEEHYQEKADRENMFLTPSTDHSPFLKGNQNSLQHKDSGSSAACPSLMPLL QLSYSDEQQL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pGW_NcoI-Eco109I
Synonyms:
pGW-NcoEco
Description:
In-fusion cloning vector with NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette; kanamycin resistance in bacteria; recombinational cloning
Comments:
NcoI (5'att) and Eco109I (3'att) sites inserted to remove death cassette. Made for use with In-Fusion cloning
Size (bp):
4008
Parent Vector:
None
Properties:
''
Author Name:
Joshua LaBaer
Andreas Rolf
Andreas Rolf
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
3332
4005
death cassette
ccdB
ccdB death cassette - removed in plasmids containing an insert
1779
2084
primer
M13 forward
M13 forward sequencing primer
536
553
primer
M13 reverse
M13 reverse sequencing primer
2269
2289
recombination site
attL1
attL1 recombinational cloning site
644
668
recombination site
attL2
attL2 recombinational cloning site
2133
2157
selectable marker
KanR
kanamycin resistance
2402
3208
selectable marker
CmR
Chloramphenicol resistance - removed in plasmids containing an insert
778
1434
