Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745734
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5163nts
Open reading frame: 1 to 5163
ATGGCTCAGTTTCCAACACCTTTTGGTGGCAGCCTGGATATCTGGGCCATAACTGTAGAG
GAAAGAGCGAAGCATGATCAGCAGTTCCATAGTTTAAAGCCAATATCTGGATTCATTACT
GGTGATCAAGCTAGAAACTTTTTTTTTCAATCTGGGTTACCTCAACCTGTTTTAGCACAG
ATATGGGCACTAGCTGACATGAATAATGATGGAAGAATGGATCAAGTGGAGTTTTCCATA
GCTATGAAACTTATCAAACTGAAGCTACAAGGATATCAGCTACCCTCTGCACTTCCCCCT
GTCATGAAACAGCAACCAGTTGCTATTTCTAGCGCACCAGCATTTGGTATGGGAGGTATC
GCCAGCATGCCACCGCTTACAGCTGTTGCTCCAGTGCCAATGGGATCCATTCCAGTTGTT
GGAATGTCTCCAACCCTAGTATCTTCTGTTCCCACAGCAGCTGTGCCCCCCCTGGCTAAC
GGGGCTCCCCCTGTTATACAACCTCTGCCTGCATTTGCTCATCCTGCAGCCACATTGCCA
AAGAGTTCTTCCTTTAGTAGATCTGGTCCAGGGTCACAACTAAACACTAAATTACAAAAG
GCACAGTCATTTGATGTGGCCAGTGTCCCACCAGTGGCAGAGTGGGCTGTTCCTCAGTCA
TCAAGACTGAAATACAGGCAATTATTCAATAGTCATGACAAAACTATGAGTGGACACTTA
ACAGGTCCCCAAGCAAGAACTATTCTTATGCAGTCAAGTTTACCACAGGCTCAGCTGGCT
TCAATATGGAATCTTTCTGACATTGATCAAGATGGAAAACTTACAGCAGAGGAATTTATC
CTGGCAATGCACCTCATTGATGTAGCTATGTCTGGCCAACCACTGCCACCTGTCCTGCCT
CCAGAATACATTCCACCTTCTTTTAGAAGAGTTCGATCTGGCAGTGGTATATCTGTCATA
AGCTCAACATCTGTAGATCAGAGGCTACCAGAGGAACCAGTTTTAGAAGATGAACAACAA
CAATTAGAAAAGAAATTACCTGTAACGTTTGAAGATAAGAAGCGGGAGAACTTTGAACGT
GGCAACCTGGAACTGGAGAAACGAAGGCAAGCTCTCCTGGAACAGCAGCGCAAGGAGCAG
GAGCGCCTGGCCCAGCTGGAGCGGGCGGAGCAGGAGAGGAAGGAGCGTGAGCGCCAGGAG
CAAGAGCGCAAAAGACAACTGGAACTGGAGAAGCAACTGGAAAAGCAGCGGGAGCTAGAA
CGGCAGAGAGAGGAGGAGAGGAGGAAAGAAATTGAGAGGCGAGAGGCTGCAAAACGGGAA
CTTGAAAGGCAACGACAACTTGAGTGGGAACGGAATCGAAGGCAAGAACTACTAAATCAA
AGAAACAAAGAACAAGAGGACATAGTTGTACTGAAAGCAAAGAAAAAGACTTTGGAATTT
GAATTAGAAGCTCTAAATGATAAAAAGCATCAACTAGAAGGGAAACTTCAAGATATCAGA
TGTCGATTGACCACCCAAAGGCAAGAAATTGAGAGCACAAACAAATCTAGAGAGTTGAGA
ATTGCCGAAATCACCCATCTACAGCAACAATTACAGGAATCTCAGCAAATGCTTGGAAGA
CTTATTCCAGAAAAACAGATACTCAATGACCAATTAAAACAAGTTCAGCAGAACAGTTTG
CACAGAGATTCACTTGTTACACTTAAAAGAGCCTTAGAAGCAAAAGAACTAGCTCGGCAG
CACCTACGAGACCAACTGGATGAAGTGGAGAAAGAAACTAGATCAAAACTACAGGAGATT
GATATTTTCAATAATCAGCTGAAGGAACTAAGAGAAATACACAATAAGCAACAACTCCAG
AAGCAAAAGTCCATGGAGGCTGAACGACTGAAACAGAAAGAACAAGAACGAAAGATCATA
GAATTAGAAAAACAAAAAGAAGAAGCCCAAAGACGAGCTCAGGAAAGGGACAAGCAGTGG
CTGGAGCATGTGCAGCAGGAGGACGAGCATCAGAGACCAAGAAAACTCCACGAAGAGGAA
AAACTGAAAAGGGAGGAGAGTGTCAAAAAGAAGGATGGCGAGGAAAAAGGCAAACAGGAA
GCACAAGACAAGCTGGGTCGGCTTTTCCATCAACACCAAGAACCAGCTAAGCCAGCTGTC
CAGGCACCCTGGTCCACTGCAGAAAAAGGTCCACTTACCATTTCTGCACAGGAAAATGTA
AAAGTGGTGTATTACCGGGCACTGTACCCCTTTGAATCCAGAAGCCATGATGAAATCACT
ATCCAGCCAGGAGACATAGTCATGGTTAAAGGGGAATGGGTGGATGAAAGCCAAACTGGA
GAACCCGGCTGGCTTGGAGGAGAATTAAAAGGAAAGACAGGGTGGTTCCCTGCAAACTAT
GCAGAGAAAATCCCAGAAAATGAGGTTCCCGCTCCAGTGAAACCAGTGACTGATTCAACA
TCTGCCCCTGCCCCCAAACTGGCCTTGCGTGAGACCCCCGCCCCTTTGGCAGTAACCTCT
TCAGAGCCCTCCACGACCCCTAATAACTGGGCCGACTTCAGCTCCACGTGGCCCACCAGC
ACGAATGAGAAACCAGAAACGGATAACTGGGATGCATGGGCAGCCCAGCCCTCTCTCACC
GTTCCAAGTGCCGGCCAGTTAAGGCAGAGGTCCGCCTTTACTCCAGCCACGGCCACTGGC
TCCTCCCCGTCTCCTGTGCTAGGCCAGGGTGAAAAGGTGGAGGGGCTACAAGCTCAAGCC
CTATATCCTTGGAGAGCCAAAAAAGACAACCACTTAAATTTTAACAAAAATGATGTCATC
ACCGTCCTGGAACAGCAAGACATGTGGTGGTTTGGAGAAGTTCAAGGTCAGAAGGGTTGG
TTCCCCAAGTCTTACGTGAAACTCATTTCAGGGCCCATAAGGAAGTCTACAAGCATGGAT
TCTGGTTCTTCAGAGAGTCCTGCTAGTCTAAAGCGAGTAGCCTCTCCAGCAGCCAAGCCG
GTCGTTTCGGGAGAAGAATTTATTGCCATGTACACTTACGAGAGTTCTGAGCAAGGAGAT
TTAACCTTTCAGCAAGGGGATGTGATTTTGGTTACCAAGAAAGATGGTGACTGGTGGACA
GGAACAGTGGGCGACAAGGCCGGAGTCTTCCCTTCTAACTATGTGAGGCTTAAAGATTCA
GAGGGCTCTGGAACTGCTGGGAAAACAGGGAGTTTAGGAAAAAAACCTGAAATTGCCCAG
GTTATTGCCTCATACACCGCCACCGGCCCCGAGCAGCTCACTCTCGCCCCTGGTCAGCTG
ATTTTGATCCGAAAAAAGAACCCAGGTGGATGGTGGGAAGGAGAGCTGCAAGCACGTGGG
AAAAAGCGCCAGATAGGCTGGTTCCCAGCTAATTATGTAAAGCTTCTAAGCCCTGGGACG
AGCAAAATCACTCCAACAGAGCCACCTAAGTCAACAGCATTAGCGGCAGTGTGCCAGGTG
ATTGGGATGTACGACTACACCGCGCAGAATGACGATGAGCTGGCCTTCAACAAGGGCCAG
ATCATCAACGTCCTCAACAAGGAGGACCCTGACTGGTGGAAAGGAGAAGTCAATGGACAA
GTGGGGCTCTTCCCATCCAATTATGTGAAGCTGACCACAGACATGGACCCAAGCCAGCAA
TGGTGTTCAGACTTACATCTCTTGGATATGTTGACCCCAACTGAAAGAAAGCGACAAGGA
TACATCCACGAGCTCATTGTCACCGAGGAGAACTATGTGAATGACCTGCAGCTGGTCACA
GAGATTTTTCAAAAACCCCTGATGGAGTCTGAGCTGCTGACAGAAAAAGAGGTTGCTATG
ATTTTTGTGAACTGGAAGGAGCTGATTATGTGTAATATCAAACTACTAAAAGCGCTGAGA
GTCCGCAAGAAGATGTCCGGGGAGAAGATGCCTGTGAAGATGATTGGAGACATCCTGAGC
GCACAGCTGCCGCACATGCAGCCCTACATCCGCTTCTGCAGCCGCCAGCTCAACGGGGCT
GCCCTGATCCAGCAGAAGACGGACGAGGCCCCAGACTTCAAGGAGTTCGTCAAAAGATTG
GCAATGGATCCTCGGTGTAGAGGGATGCCACTCTCTAGTTTTATACTGAAGCCTATGCAA
CGGGTAACAAGATACCCACTGATCATTAAAAATATCCTGGAAAACACCCCTGAAAACCAC
CCGGACCACAGCCACTTGAAGCACGCCCTGGAGAAGGCGGAAGAGCTCTGTTCCCAGGTG
AACGAAGGGGTGCGGGAGAAGGAGAACTCTGACCGGCTGGAGTGGATCCAGGCCCACGTG
CAGTGTGAAGGCCTGTCTGAGCAACTTGTGTTCAATTCAGTGACCAATTGCTTGGGGCCG
CGCAAATTTCTGCACAGTGGGAAGCTCTACAAGGCCAAGAGCAACAAGGAGCTGTATGGC
TTCCTTTTCAACGACTTCCTCCTGCTGACTCAGATCACGAAGCCTTTGGGGTCTTCTGGC
ACCGACAAAGTCTTCAGCCCCAAATCAAACCTGCAGTATAAAATGTATAAAACACCTATT
TTCCTAAATGAGGTTCTAGTAAAATTACCCACCGACCCTTCTGGAGACGAGCCCATCTTC
CACATCTCCCACATTGACCGCGTCTATACTCTCCGAGCAGAAAGCATAAATGAAAGGACT
GCCTGGGTGCAGAAAATCAAAGCTGCTTCTGAACTCTACATAGAGACTGAGAAAAAGAAG
CGCGAGAAAGCGTACCTGGTCCGTTCCCAAAGGGCAACAGGCATTGGAAGGTTGATGGTG
AACGTGGTTGAAGGCATCGAGTTGAAACCCTGTCGGTCACATGGAAAGAGCAACCCGTAC
TGTGAGGTGACCATGGGTTCCCAGTGCCACATCACCAAGACGATCCAGGACACTCTGAAC
CCCAAGTGGAATTCCAACTGCCAGTTCTTCATCCGAGACCTGGAGCAGGAAGTCCTCTGC
ATCACTGTGTTCGAGAGGGACCAGTTCTCACCAGATGATTTTTTGGGTCGGACGGAGATC
CGTGTGGCGGACATCAAGAAAGACCAGGGCTCCAAAGGTCCAGTTACGAAGTGTCTTCTG
CTGCACGAAGTCCCCACGGGAGAGATTGTGGTCCGCTTGGACCTGCAGTTGTTTGATGAG
CCG
Protein sequence predicted based on nucleotide sequence
MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQ IWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGI ASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLP KSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHL TGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLP PEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFER GNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELE RQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEF ELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGR LIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEI DIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQW LEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAV QAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTG EPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTS SEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATG SSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGW FPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGD LTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQ VIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGT SKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQ VGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVT EIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILS AQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCRGMPLSSFILKPMQ RVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHV QCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSG TDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERT AWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPY CEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEI RVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
