Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745859
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5454nts
Open reading frame: 1 to 5454
ATGGAGCCTTGGTCCTCCAGGTGGAAGACCAAGAGGTGGCTGTGGGATTTCACCGTGACA
ACCCTGGCCCTGACCTTCCTGTTCCAGGCCAGAGAGGTGAGAGGCGCCGCCCCCGTGGAT
GTGCTGAAAGCCCTGGATTTTCACAATTCTCCCGAGGGCATCTCCAAAACCACCGGCTTT
TGCACAAACAGAAAGAATTCTAAAGGCTCCGATACCGCCTACAGAGTGTCCAAGCAGGCC
CAGCTGAGCGCCCCAACCAAGCAGCTGTTCCCCGGCGGCACCTTCCCCGAGGACTTTTCC
ATCCTCTTTACAGTGAAGCCAAAAAAGGGCATTCAGTCTTTCCTGCTGTCTATCTACAAT
GAGCACGGCATCCAGCAGATTGGCGTGGAGGTGGGCAGATCCCCTGTGTTTCTCTTTGAG
GACCACACCGGAAAGCCTGCCCCAGAGGACTATCCCCTGTTCAGAACCGTGAACATCGCC
GACGGCAAGTGGCACCGGGTGGCCATCAGCGTGGAGAAGAAAACTGTGACCATGATCGTG
GATTGTAAGAAGAAGACCACCAAGCCACTGGATAGAAGCGAGAGAGCCATTGTGGATACC
AATGGCATCACCGTGTTTGGCACCAGGATTCTGGACGAGGAGGTGTTTGAGGGGGACATT
CAGCAGTTTCTGATCACCGGCGATCCCAAGGCCGCCTACGACTACTGTGAGCACTACAGC
CCAGACTGCGACTCTTCCGCCCCCAAGGCTGCTCAGGCTCAGGAGCCTCAGATCGATGAG
AAGAAGAAATCCAATTTCAAAAAGAAGATGAGGACCGTGGCCACCAAGTCCAAGGAGAAG
TCCAAAAAGTTTACCCCCCCCAAGTCTGAGAAGTTTTCCTCCAAGAAGAAGAAGAGCTAT
CAGGCCTCCGCCAAGGCCAAACTGGGGGTGAAGGCCAACATCGTGGATGATTTTCAGGAG
TACAACTACGGCACAATGGAGAGCTACCAGACCGAAGCCCCTAGGCACGTGTCTGGCACC
AATGAGCCAAATCCCGTGGAGGAAATCTTTACCGAGGAGTACCTGACCGGCGAGGATTAT
GATTCCCAGAGGAAGAATTCCGAGGATACCCTGTACGAGAACAAGGAGATCGACGGCAGG
GATTCTGATCTGCTGGTGGACGGCGATCTGGGCGAGTACGATTTTTACGAGTACAAGGAG
TACGAGGATAAGCCAACCAGCCCCCCTAATGAGGAGTTCGGCCCAGGCGTGCCAGCCGAA
ACCGATATCACCGAGACAAGCATCAATGGCCACGGCGCCTACGGAGAGAAGGGACAGAAA
GGAGAGCCCGCCGTGGTTGAGCCCGGCATGCTGGTGGAGGGACCACCAGGACCCGCCGGA
CCTGCAGGCATTATGGGCCCTCCAGGACTTCAGGGCCCCACCGGACCACCTGGTGACCCT
GGCGATAGGGGCCCTCCAGGAAGGCCCGGCTTACCAGGGGCTGATGGCTTGCCTGGCCCT
CCCGGAACCATGCTGATGCTGCCCTTCCGCTACGGCGGCGATGGCTCCAAAGGACCAACC
ATCTCTGCCCAGGAAGCCCAGGCCCAGGCCATTCTGCAGCAGGCCCGGATTGCTCTTAGA
GGCCCACCTGGACCAATGGGACTGACCGGAAGACCCGGCCCCGTGGGCGGCCCCGGCTCT
TCAGGAGCTAAAGGAGAGAGTGGAGATCCAGGCCCTCAGGGCCCTAGAGGCGTTCAGGGG
CCTCCAGGCCCAACCGGAAAACCTGGAAAAAGAGGCCGGCCAGGCGCCGACGGCGGCAGA
GGAATGCCAGGGGAGCCTGGGGCCAAGGGAGATAGAGGCTTTGATGGACTGCCCGGCCTG
CCAGGAGACAAGGGCCACAGGGGCGAGCGCGGCCCTCAGGGCCCTCCAGGACCTCCTGGC
GATGACGGCATGAGGGGAGAAGATGGAGAGATCGGACCTAGAGGACTCCCCGGAGAAGCC
GGCCCCAGAGGATTACTGGGCCCAAGGGGAACTCCCGGCGCTCCAGGGCAGCCTGGCATG
GCAGGAGTGGATGGACCCCCCGGACCAAAGGGCAATATGGGCCCTCAGGGCGAGCCTGGA
CCTCCCGGCCAACAGGGCAATCCAGGCCCTCAGGGACTGCCTGGCCCCCAGGGACCAATT
GGCCCTCCTGGAGAAAAGGGACCCCAGGGCAAACCTGGCTTAGCTGGACTGCCTGGCGCC
GATGGGCCTCCTGGCCATCCTGGGAAAGAGGGACAGTCTGGCGAGAAAGGAGCCCTGGGC
CCCCCTGGCCCACAGGGCCCCATCGGCTACCCAGGCCCTAGGGGCGTGAAGGGAGCTGAT
GGCGTCAGAGGCCTGAAGGGATCCAAAGGCGAAAAGGGCGAAGATGGCTTTCCAGGCTTC
AAAGGCGACATGGGCCTGAAGGGCGACAGAGGAGAGGTGGGCCAGATCGGCCCAAGAGGG
GAGGACGGCCCTGAGGGACCCAAAGGAAGAGCTGGCCCAACCGGCGACCCCGGCCCTTCA
GGACAAGCCGGCGAGAAGGGCAAGCTGGGAGTCCCAGGACTGCCAGGCTACCCCGGAAGA
CAGGGCCCCAAGGGCTCCACTGGCTTCCCTGGCTTTCCTGGCGCCAATGGCGAGAAAGGC
GCAAGAGGCGTGGCCGGCAAACCAGGCCCTCGGGGGCAACGGGGCCCAACCGGGCCTAGA
GGCTCCAGAGGAGCAAGAGGACCCACTGGCAAACCCGGGCCAAAGGGCACCTCTGGCGGA
GATGGCCCTCCTGGCCCTCCAGGCGAGAGGGGCCCTCAAGGACCTCAGGGACCCGTTGGA
TTCCCTGGGCCAAAAGGCCCTCCTGGACCCCCAGGCAAGGATGGCCTGCCAGGACACCCT
GGGCAGCGCGGGGAGACTGGATTTCAGGGCAAGACCGGCCCTCCTGGCCCAGGAGGAGTG
GTTGGACCACAGGGACCTACCGGCGAGACTGGCCCTATCGGCGAAAGAGGCCACCCTGGC
CCTCCTGGCCCTCCTGGCGAGCAGGGACTTCCTGGCGCCGCAGGCAAAGAAGGAGCCAAG
GGCGACCCAGGGCCTCAAGGCATCTCTGGCAAAGACGGCCCAGCCGGACTCCGGGGCTTC
CCAGGGGAGAGAGGACTGCCTGGAGCTCAGGGAGCACCTGGACTTAAAGGCGGCGAGGGT
CCCCAGGGACCACCAGGCCCAGTTGGCTCCCCAGGAGAGAGAGGGTCAGCTGGCACAGCT
GGCCCCATTGGCTTACCCGGCAGGCCTGGCCCTCAGGGACCACCTGGCCCCGCTGGCGAG
AAAGGCGCTCCTGGCGAAAAAGGCCCCCAGGGACCTGCAGGAAGAGATGGCGTCCAGGGC
CCTGTGGGGCTGCCAGGGCCAGCTGGACCTGCCGGATCTCCTGGCGAAGACGGCGACAAG
GGCGAGATCGGCGAGCCAGGCCAAAAAGGCAGCAAGGGCGACAAGGGCGAAAATGGCCCT
CCCGGCCCACCAGGTCTGCAGGGACCAGTGGGCGCCCCTGGAATCGCTGGAGGCGACGGC
GAACCAGGACCCAGAGGCCAGCAGGGCATGTTTGGGCAGAAGGGCGATGAGGGCGCAAGA
GGCTTCCCCGGGCCTCCCGGCCCAATCGGCCTGCAGGGTCTGCCAGGCCCACCTGGCGAG
AAAGGCGAGAATGGAGATGTTGGCCCCATGGGACCCCCTGGTCCCCCAGGGCCAAGAGGC
CCTCAGGGCCCCAATGGAGCTGACGGACCCCAGGGACCTCCAGGCTCTGTTGGATCTGTG
GGAGGCGTGGGGGAAAAAGGCGAGCCCGGCGAAGCCGGCAACCCAGGACCTCCTGGCGAG
GCCGGCGTTGGCGGCCCTAAGGGAGAAAGAGGAGAAAAAGGGGAAGCTGGTCCACCTGGA
GCCGCCGGCCCTCCAGGCGCCAAGGGGCCCCCAGGAGATGATGGCCCTAAAGGCAACCCC
GGCCCTGTGGGTTTTCCTGGCGATCCAGGTCCTCCTGGCGAACCTGGCCCTGCCGGACAG
GACGGCGTGGGCGGAGACAAAGGCGAGGATGGCGATCCTGGACAACCCGGCCCTCCTGGC
CCATCAGGAGAGGCTGGGCCACCAGGCCCTCCTGGCAAGAGAGGCCCTCCAGGAGCTGCC
GGAGCAGAGGGCAGACAAGGCGAGAAAGGCGCTAAGGGCGAGGCAGGCGCCGAAGGCCCT
CCCGGCAAAACCGGACCCGTGGGCCCTCAGGGACCTGCTGGCAAGCCTGGCCCAGAAGGA
CTTAGGGGAATCCCCGGCCCAGTGGGAGAGCAGGGCTTGCCCGGAGCTGCTGGCCAGGAC
GGACCACCCGGACCTATGGGACCTCCTGGCCTGCCTGGCCTGAAAGGCGATCCCGGCTCC
AAGGGGGAAAAGGGACACCCCGGCCTGATCGGCCTGATTGGCCCCCCAGGCGAACAGGGC
GAGAAAGGGGACAGAGGGCTGCCCGGAACTCAGGGATCTCCCGGAGCCAAAGGGGATGGC
GGAATTCCTGGTCCTGCTGGCCCCCTGGGACCACCTGGCCCTCCAGGCCTTCCCGGCCCT
CAGGGCCCAAAGGGAAATAAGGGATCTACAGGCCCCGCCGGCCAGAAGGGAGACAGTGGC
CTGCCCGGCCCTCCTGGCTCTCCAGGCCCCCCCGGCGAGGTGATTCAGCCTCTGCCAATC
TTGTCCTCCAAAAAAACCAGAAGACACACTGAGGGCATGCAGGCCGATGCCGATGATAAT
ATCCTGGATTACTCCGATGGCATGGAGGAGATCTTCGGCTCCCTGAATTCCCTGAAGCAG
GACATCGAGCACATGAAGTTTCCAATGGGCACCCAGACCAATCCAGCCAGAACCTGTAAG
GACCTGCAGCTGAGCCACCCTGACTTCCCAGATGGCGAGTATTGGATCGACCCTAACCAG
GGCTGCTCCGGCGATTCCTTCAAGGTGTACTGTAACTTCACATCTGGCGGCGAAACCTGC
ATCTACCCAGACAAGAAGTCCGAGGGCGTGAGAATCTCATCATGGCCAAAGGAGAAACCC
GGCAGCTGGTTTAGCGAGTTTAAGAGGGGAAAGCTGCTGTCCTACCTGGATGTGGAGGGC
AATTCCATCAATATGGTGCAGATGACATTCCTGAAGCTGCTGACCGCCTCTGCCCGGCAG
AATTTCACCTACCACTGTCACCAGTCCGCCGCCTGGTACGATGTGTCCTCAGGCAGCTAT
GACAAGGCCCTGCGGTTCCTGGGCTCCAATGATGAGGAGATGTCCTACGACAATAATCCT
TTTATCAAGACCCTGTATGATGGCTGTGCCTCCAGAAAGGGCTACGAGAAGACCGTGATC
GAGATCAATACCCCAAAGATCGATCAGGTGCCTATCGTGGACGTGATGATCAATGACTTT
GGCGACCAGAATCAGAAGTTCGGCTTTGAGGTGGGACCTGTGTGTTTTCTGGGC
Protein sequence predicted based on nucleotide sequence
MEPWSSRWKTKRWLWDFTVTTLALTFLFQAREVRGAAPVDVLKALDFHNSPEGISKTTGF CTNRKNSKGSDTAYRVSKQAQLSAPTKQLFPGGTFPEDFSILFTVKPKKGIQSFLLSIYN EHGIQQIGVEVGRSPVFLFEDHTGKPAPEDYPLFRTVNIADGKWHRVAISVEKKTVTMIV DCKKKTTKPLDRSERAIVDTNGITVFGTRILDEEVFEGDIQQFLITGDPKAAYDYCEHYS PDCDSSAPKAAQAQEPQIDEKKKSNFKKKMRTVATKSKEKSKKFTPPKSEKFSSKKKKSY QASAKAKLGVKANIVDDFQEYNYGTMESYQTEAPRHVSGTNEPNPVEEIFTEEYLTGEDY DSQRKNSEDTLYENKEIDGRDSDLLVDGDLGEYDFYEYKEYEDKPTSPPNEEFGPGVPAE TDITETSINGHGAYGEKGQKGEPAVVEPGMLVEGPPGPAGPAGIMGPPGLQGPTGPPGDP GDRGPPGRPGLPGADGLPGPPGTMLMLPFRYGGDGSKGPTISAQEAQAQAILQQARIALR GPPGPMGLTGRPGPVGGPGSSGAKGESGDPGPQGPRGVQGPPGPTGKPGKRGRPGADGGR GMPGEPGAKGDRGFDGLPGLPGDKGHRGERGPQGPPGPPGDDGMRGEDGEIGPRGLPGEA GPRGLLGPRGTPGAPGQPGMAGVDGPPGPKGNMGPQGEPGPPGQQGNPGPQGLPGPQGPI GPPGEKGPQGKPGLAGLPGADGPPGHPGKEGQSGEKGALGPPGPQGPIGYPGPRGVKGAD GVRGLKGSKGEKGEDGFPGFKGDMGLKGDRGEVGQIGPRGEDGPEGPKGRAGPTGDPGPS GQAGEKGKLGVPGLPGYPGRQGPKGSTGFPGFPGANGEKGARGVAGKPGPRGQRGPTGPR GSRGARGPTGKPGPKGTSGGDGPPGPPGERGPQGPQGPVGFPGPKGPPGPPGKDGLPGHP GQRGETGFQGKTGPPGPGGVVGPQGPTGETGPIGERGHPGPPGPPGEQGLPGAAGKEGAK GDPGPQGISGKDGPAGLRGFPGERGLPGAQGAPGLKGGEGPQGPPGPVGSPGERGSAGTA GPIGLPGRPGPQGPPGPAGEKGAPGEKGPQGPAGRDGVQGPVGLPGPAGPAGSPGEDGDK GEIGEPGQKGSKGDKGENGPPGPPGLQGPVGAPGIAGGDGEPGPRGQQGMFGQKGDEGAR GFPGPPGPIGLQGLPGPPGEKGENGDVGPMGPPGPPGPRGPQGPNGADGPQGPPGSVGSV GGVGEKGEPGEAGNPGPPGEAGVGGPKGERGEKGEAGPPGAAGPPGAKGPPGDDGPKGNP GPVGFPGDPGPPGEPGPAGQDGVGGDKGEDGDPGQPGPPGPSGEAGPPGPPGKRGPPGAA GAEGRQGEKGAKGEAGAEGPPGKTGPVGPQGPAGKPGPEGLRGIPGPVGEQGLPGAAGQD GPPGPMGPPGLPGLKGDPGSKGEKGHPGLIGLIGPPGEQGEKGDRGLPGTQGSPGAKGDG GIPGPAGPLGPPGPPGLPGPQGPKGNKGSTGPAGQKGDSGLPGPPGSPGPPGEVIQPLPI LSSKKTRRHTEGMQADADDNILDYSDGMEEIFGSLNSLKQDIEHMKFPMGTQTNPARTCK DLQLSHPDFPDGEYWIDPNQGCSGDSFKVYCNFTSGGETCIYPDKKSEGVRISSWPKEKP GSWFSEFKRGKLLSYLDVEGNSINMVQMTFLKLLTASARQNFTYHCHQSAAWYDVSSGSY DKALRFLGSNDEEMSYDNNPFIKTLYDGCASRKGYEKTVIEINTPKIDQVPIVDVMINDF GDQNQKFGFEVGPVCFLG
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
