Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745866
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6012nts
Open reading frame: 1 to 6012
ATGGTAAAACTCGCAAACCCGCTTTATACTGAGTGGATTTTGGAGGCCATCAAAAAAGTG
AAAAAGCAGAAACAGCGTCCTTCAGAAGAAAGGATATGCAATGCTGTGTCTTCCTCCCAT
GGCTTGGATCGTAAAACTGTTTTAGAACAATTGGAGTTGAGCGTTAAAGATGGAACAATT
CTGAAAGTCTCAAATAAGGGACTCAATTCCTATAAAGATCCCGATAATCCTGGGCGAATA
GCACTTCCTAAGCCTCGGAACCATGGAAAATTGGATAATAAACAAAATGTGGATTGGAAT
AAACTGATAAAGCGGGCAGTTGAGGGCTTGGCAGAGTCTGGTGGCTCAACTTTGAAAAGC
ATTGAACGTTTTTTGAAAGGTCAGAAGGATGTGTCTGCATTATTCGGAGGCAGTGCTGCC
TCTGGCTTTCACCAGCAGTTACGATTGGCTATCAAACGTGCCATTGGCCACGGCAGACTT
CTTAAAGATGGACCTCTCTATCGGCTCAACACTAAAGCAACCAACGTGGATGGGAAAGAG
AGTTGTGAGTCTCTTTCCTGTTTACCTCCAGTGTCCCTTCTTCCACATGAAAAGGATAAG
CCGGTTGCTGAACCAATCCCCATCTGTAGTTTCTGTCTTGGTACAAAAGAACAAAACCGA
GAAAAGAAGCCAGAGGAACTCATCTCCTGTGCCGACTGTGGCAACAGTGGCCATCCCTCC
TGTTTAAAGTTTTCCCCTGAACTAACGGTTCGAGTGAAGGCCTTAAGGTGGCAGTGCATC
GAGTGTAAAACATGCAGCTCCTGTCGAGATCAAGGCAAAAATGCGGATAACATGCTCTTT
TGTGATTCATGTGACCGAGGTTTTCACATGGAGTGTTGTGATCCGCCACTCACCCGTATG
CCAAAAGGCATGTGGATATGTCAAATATGTCGACCTCGGAAAAAAGGACGAAAACTTCTA
CAAAAGAAGGCAGCACAGATAAAACGGCGCTATACTAATCCAATAGGACGTCCAAAAAAC
AGGTTAAAGAAACAAAACACGGTATCAAAAGGTCCCTTCAGCAAAGTTCGAACTGGCCCT
GGAAGGGGTAGGAAACGAAAAATCACTCTTTCCAGCCAATCAGCATCATCATCATCAGAA
GAAGGATATTTAGAGCGGATAGATGGCTTGGACTTCTGCAGAGATAGCAATGTCTCCTTG
AAGTTCAACAAGAAAACCAAAGGGCTCATTGATGGCCTTACCAAATTTTTTACCCCTTCC
CCTGATGGGCGGAAAGCTCGCGGGGAAGTGGTGGACTACTCTGAGCAATATCGAATCAGA
AAGAGGGGCAACAGGAAATCAAGCACTTCAGATTGGCCCACAGACAATCAGGATGGCTGG
GATGGCAAACAAGAAAATGAGGAGCGACTTTTTGGGAGCCAGGAAATCATGACTGAGAAA
GATATGGAATTGTTTCGTGATATCCAAGAACAAGCACTGCAGAAAGTTGGAGTGACTGGT
CCCCCTGATCCACAAGTCCGCTGTCCCTCTGTCATTGAGTTTGGGAAGTATGAAATTCAC
ACCTGGTACTCCTCCCCATATCCTCAAGAATACTCAAGGCTGCCCAAATTGTATCTTTGT
GAATTTTGTCTAAAATATATGAAAAGCAGAACTATTCTGCAGCAGCACATGAAGAAATGT
GGTTGGTTCCATCCTCCTGCCAATGAGATTTACAGAAAGAATAACATTTCTGTCTTTGAG
GTTGATGGGAATGTGAGTACCATTTATTGTCAAAACCTGTGTCTTTTGGCAAAGTTGTTT
CTTGACCACAAAACCCTCTATTACGATGTGGAGCCATTTCTTTTTTATGTACTAACACAG
AATGATGTCAAGGGCTGCCACCTTGTTGGCTACTTTTCTAAGGAAAAGCACTGCCAACAG
AAGTACAATGTTTCCTGTATAATGATTCTGCCTCAATACCAGCGTAAGGGCTATGGCAGA
TTTCTCATCGATTTCAGCTATTTGTTATCAAAGCGTGAAGGCCAAGCAGGGTCCCCAGAG
AAACCGTTATCTGATCTGGGTCGGCTTTCCTACATGGCATATTGGAAAAGTGTAATATTG
GAGTGCCTTTATCACCAAAATGACAAGCAGATCAGCATTAAGAAGTTAAGCAAGTTGACT
GGAATCTGCCCTCAAGACATCACTTCTACACTCCACCACCTACGAATGCTGGACTTCCGT
AGTGACCAATTTGTGATTATCCGCCGGGAAAAACTTATCCAGGATCACATGGCAAAGCTT
CAGCTGAATTTGCGACCTGTAGATGTAGATCCAGAATGTTTGCGCTGGACTCCAGTCATA
GTGTCCAACTCCGTCGTCTCAGAGGAGGAAGAAGAGGAGGCTGAGGAAGGAGAAAACGAA
GAGCCACAGTGCCAGGAAAGAGAATTAGAGATCAGTGTGGGAAAGTCTGTGTCTCATGAG
AACAAAGAACAAGATTCTTATTCAGTAGAAAGTGAAAAGAAACCAGAAGTTATGGCTCCA
GTCAGTTCTACACGTTTGAGCAAACAAGTCCTTCCTCATGATAGTCTTCCTGCAAATAGC
CAGCCATCTAGGAGGGGCCGCTGGGGGAGGAAGAACAGAAAAACCCAGGAACGTTTTGGT
GATAAAGATTCTAAACTGCTCTTGGAAGAGACGTCTTCAGCTCCTCAGGAACAATATGGA
GAATGTGGGGAGAAATCAGAAGCCACCCAGGAACAATACACTGAAAGTGAAGAACAGCTG
GTGGCTTCTGAGGAGCAGCCAAGCCAGGACGGGAAACCTGACCTTCCCAAGAGAAGACTC
AGTGAGGGGGTTGAGCCCTGGCGAGGACAGCTCAAGAAAAGCCCTGAGGCTCTGAAGTGC
AGATTAACAGAAGGAAGTGAGAGGCTGCCCCGTCGCTACAGTGAGGGTGACAGAGCTGTC
CTCAGGGGCTTCAGTGAGAGCAGCGAGGAGGAGGAGGAGCCGGAAAGCCCTCGGTCAAGC
TCGCCACCAATTCTCACAAAGCCCACGCTGAAGCGAAAGAAACCATTTCTCCACCGAAGG
AGGAGAGTCCGAAAGCGCAAACACCACAATAGCAGTGTAGTCACAGAAACTATTTCTGAG
ACAACTGAAGTGTTAGATGAACCTTTTGAAGATTCTGACTCCGAGAGGCCAATGCCAAGA
TTAGAACCCACGTTTGAGATCGATGAAGAAGAGGAGGAAGAGGATGAAAATGAACTTTTC
CCTAGAGAATACTTCCGTCGTTTGTCTTCGCAGGATGTACTCAGGTGTCAGTCCTCTTCT
AAGAGGAAGTCTAAAGATGAAGAAGAAGATGAAGAGTCAGATGATGCCGATGACACTCCT
ATCTTGAAGCCAGTATCTCTTTTGCGAAAACGTGATGTGAAGAATTCTCCTCTTGAGCCA
GATACATCCACACCTTTGAAAAAGAAAAAGGGATGGCCCAAAGGCAAGAGCCGCAAACCA
ATCCACTGGAAGAAAAGACCTGGTCGAAAACCAGGATTTAAGTTGAGTAGGGAAATCATG
CCAGTTTCTACTCAAGCATGCGTCATTGAGCCCATCGTTTCCATTCCTAAAGCTGGACGT
AAACCCAAGATCCAGGAGAGTGAAGAAACTGTTGAGCCAAAAGAAGACATGCCCCTACCC
GAGGAGAGGAAGGAGGAGGAGGAGATGCAAGCAGAGGCAGAAGAGGCTGAAGAGGGTGAG
GAAGAGGATGCAGCTAGCAGTGAAGTCCCAGCAGCCTCTCCAGCAGACAGCAGCAATAGT
CCTGAAACCGAAACAAAGGAGCCTGAGGTGGAGGAGGAAGAAGAGAAGCCCCGTGTCTCA
GAGGAGCAGAGGCAGTCAGAGGAGGAGCAGCAGGAATTAGAGGAGCCAGAGCCAGAGGAG
GAGGAAGATGCAGCTGCAGAGACTGCCCAGAATGACGACCACGACGCTGATGATGAGGAT
GATGGCCACCTGGAGTCCACAAAGAAAAAGGAGCTAGAGGAACAGCCCACGAGGGAAGAT
GTCAAGGAGGAGCCTGGTGTTCAAGAGTCTTTTTTAGATGCTAATATGCAGAAGAGTAGG
GAAAAGATAAAGGATAAAGAGGAAACCGAGCTGGATTCCGAAGAGGAGCAGCCTTCCCAT
GACACGTCCGTGGTGTCAGAGCAGATGGCTGGGTCTGAGGACGACCACGAAGAAGACTCC
CACACTAAGGAAGAGTTAATCGAATTAAAAGAGGAGGAAGAGATTCCTCATAGTGAGCTG
GATCTCGAAACTGTACAGGCAGTGCAGTCTTTGACTCAAGAAGAAAGCAGTGAGCATGAG
GGCGCCTACCAGGACTGTGAGGAAACTCTTGCGGCGTGTCAGACCCTGCAGAGTTACACC
CAGGCTGACGAGGACCCTCAGATGTCCATGGTTGAAGACTGTCATGCGTCAGAACATAAT
AGCCCTATCTCCTCCGTTCAGTCTCACCCCAGCCAGTCAGTCCGTTCGGTCAGCAGTCCC
AACGTGCCTGCCCTTGAGAGTGGCTACACCCAGATCAGCCCAGAACAAGGATCCCTGTCC
GCACCCTCTATGCAGAACATGGAAACCAGCCCCATGATGGATGTGCCTTCCGTATCCGAC
CACTCTCAGCAGGTCGTGGACTCCGGCTTCAGTGACCTGGGCAGCATTGAGAGCACCACT
GAAAACTATGAGAACCCAAGCAGTTACGACTCCACGATGGGCGGCAGCATCTGTGGGAAC
AGTTCTTCCCAGAGCAGCTGCTCCTACGGTGGGCTGTCGTCCTCCAGCAGCCTCACCCAG
AGCAGCTGTGTGGTCACTCAGCAGATGGCTAGCATGGGCAGCAGCTGCAGCATGATGCAG
CAGAGCAGCGTCCAGCCTGCTGCCAACTGCAGCATCAAGTCACCTCAGAGTTGCGTGGTG
GAGAGGCCTCCCAGTAACCAGCAGCAACAACCGCCACCACCTCCTCCACAGCAGCCACAG
CCGCCGCCGCCACAACCACAACCAGCACCACAACCTCCACCACCTCAGCAACAGCCGCAA
CAACAACCTCAGCCTCAGCCCCAGCAGCCTCCACCCCCACCTCCTCCTCAGCAGCAGCCC
CCTCTGTCACAGTGTAGCATGAATAACAGTTTCACCCCAGCTCCTATGATCATGGAGATA
CCAGAATCTGGAAGCACTGGGAACATAAGTATCTATGAGAGAATTCCAGGGGATTTTGGT
GCCGGCAGCTACTCTCAACCATCAGCCACCTTCAGCCTAGCCAAGCTGCAGCAGCTGACC
AACACCATTATGGACCCTCATGCCATGCCTTATAGCCATTCTCCTGCTGTGACTTCCTAT
GCAACCAGTGTTTCTCTGTCCAATACAGGACTCGCTCAGCTGGCTCCATCCCATCCCTTA
GCTGGGACTCCTCAAGCACAAGCCACCATGACGCCACCCCCAAACTTGGCATCCACTACC
ATGAACCTGACATCTCCTCTGCTTCAGTGCAACATGTCTGCCACCAACATTGGCATTCCT
CACACGCAGAGATTGCAAGGGCAAATGCCAGTGAAGGGGCACATTTCCATCCGCTCCAAG
TCTGCGCCACTGCCCTCAGCGGCTGCTCACCAGCAGCAGCTGTATGGCCGTAGTCCATCG
GCAGTTGCTATGCAGGCTGGCCCTCGCGCACTGGCTGTTCAGCGTGGCATGAACATGGGG
GTTAATCTGATGCCTACTCCCGCCTATAATGTCAATTCCATGAATATGAACACCTTGAAT
GCCATGAACAGCTATCGAATGACACAGCCCATGATGAACAGCAGTTACCATAGTAACCCT
GCCTACATGAACCAGACAGCACAGTATCCTATGCAGATGCAGATGGGAATGATGGGGAGC
CAGGCCTATACCCAGCAGCCTATGCAGCCTAACCCTCATGGGAACATGATGTACACAGGC
CCCTCCCATCACAGCTACATGAACGCTGCTGGCGTGCCCAAGCAGTCACTCAACGGACCT
TACATGAGAAGA
Protein sequence predicted based on nucleotide sequence
MVKLANPLYTEWILEAIKKVKKQKQRPSEERICNAVSSSHGLDRKTVLEQLELSVKDGTI LKVSNKGLNSYKDPDNPGRIALPKPRNHGKLDNKQNVDWNKLIKRAVEGLAESGGSTLKS IERFLKGQKDVSALFGGSAASGFHQQLRLAIKRAIGHGRLLKDGPLYRLNTKATNVDGKE SCESLSCLPPVSLLPHEKDKPVAEPIPICSFCLGTKEQNREKKPEELISCADCGNSGHPS CLKFSPELTVRVKALRWQCIECKTCSSCRDQGKNADNMLFCDSCDRGFHMECCDPPLTRM PKGMWICQICRPRKKGRKLLQKKAAQIKRRYTNPIGRPKNRLKKQNTVSKGPFSKVRTGP GRGRKRKITLSSQSASSSSEEGYLERIDGLDFCRDSNVSLKFNKKTKGLIDGLTKFFTPS PDGRKARGEVVDYSEQYRIRKRGNRKSSTSDWPTDNQDGWDGKQENEERLFGSQEIMTEK DMELFRDIQEQALQKVGVTGPPDPQVRCPSVIEFGKYEIHTWYSSPYPQEYSRLPKLYLC EFCLKYMKSRTILQQHMKKCGWFHPPANEIYRKNNISVFEVDGNVSTIYCQNLCLLAKLF LDHKTLYYDVEPFLFYVLTQNDVKGCHLVGYFSKEKHCQQKYNVSCIMILPQYQRKGYGR FLIDFSYLLSKREGQAGSPEKPLSDLGRLSYMAYWKSVILECLYHQNDKQISIKKLSKLT GICPQDITSTLHHLRMLDFRSDQFVIIRREKLIQDHMAKLQLNLRPVDVDPECLRWTPVI VSNSVVSEEEEEEAEEGENEEPQCQERELEISVGKSVSHENKEQDSYSVESEKKPEVMAP VSSTRLSKQVLPHDSLPANSQPSRRGRWGRKNRKTQERFGDKDSKLLLEETSSAPQEQYG ECGEKSEATQEQYTESEEQLVASEEQPSQDGKPDLPKRRLSEGVEPWRGQLKKSPEALKC RLTEGSERLPRRYSEGDRAVLRGFSESSEEEEEPESPRSSSPPILTKPTLKRKKPFLHRR RRVRKRKHHNSSVVTETISETTEVLDEPFEDSDSERPMPRLEPTFEIDEEEEEEDENELF PREYFRRLSSQDVLRCQSSSKRKSKDEEEDEESDDADDTPILKPVSLLRKRDVKNSPLEP DTSTPLKKKKGWPKGKSRKPIHWKKRPGRKPGFKLSREIMPVSTQACVIEPIVSIPKAGR KPKIQESEETVEPKEDMPLPEERKEEEEMQAEAEEAEEGEEEDAASSEVPAASPADSSNS PETETKEPEVEEEEEKPRVSEEQRQSEEEQQELEEPEPEEEEDAAAETAQNDDHDADDED DGHLESTKKKELEEQPTREDVKEEPGVQESFLDANMQKSREKIKDKEETELDSEEEQPSH DTSVVSEQMAGSEDDHEEDSHTKEELIELKEEEEIPHSELDLETVQAVQSLTQEESSEHE GAYQDCEETLAACQTLQSYTQADEDPQMSMVEDCHASEHNSPISSVQSHPSQSVRSVSSP NVPALESGYTQISPEQGSLSAPSMQNMETSPMMDVPSVSDHSQQVVDSGFSDLGSIESTT ENYENPSSYDSTMGGSICGNSSSQSSCSYGGLSSSSSLTQSSCVVTQQMASMGSSCSMMQ QSSVQPAANCSIKSPQSCVVERPPSNQQQQPPPPPPQQPQPPPPQPQPAPQPPPPQQQPQ QQPQPQPQQPPPPPPPQQQPPLSQCSMNNSFTPAPMIMEIPESGSTGNISIYERIPGDFG AGSYSQPSATFSLAKLQQLTNTIMDPHAMPYSHSPAVTSYATSVSLSNTGLAQLAPSHPL AGTPQAQATMTPPPNLASTTMNLTSPLLQCNMSATNIGIPHTQRLQGQMPVKGHISIRSK SAPLPSAAAHQQQLYGRSPSAVAMQAGPRALAVQRGMNMGVNLMPTPAYNVNSMNMNTLN AMNSYRMTQPMMNSSYHSNPAYMNQTAQYPMQMQMGMMGSQAYTQQPMQPNPHGNMMYTG PSHHSYMNAAGVPKQSLNGPYMRR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
