Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745911
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 8691nts
Open reading frame: 1 to 8691
ATGACAGATCCAATGATGGACTTTTTTGATGATGCCAATCTTTTTGGTGAGACCTTAGAA
GGTTTGTCAGATGATGCATTTGTACAACCAGGACCTGTTTCACTAGTTGATGAATTGAAT
TTGGGTGCAGAATTTGAACCGTTGCACATAGATTCACTGAACCATGTTCAAGGTACTCCA
ACACATCAGAAGATGACTGATTTTGAACAGTTAAATCAATTTGATTCAATAAAATTTCAC
CATGTTAATCAATCTTTTGGTAGCCCAGCTGAACATGTGTTATCTCCACACTCTCAGTTT
AATTGTTCTCCAATCCATCCCCAAAACCAACCCAATGGTTTGTTTCCAGATGTATCAGAT
GGCAGTCCAATGTGGGGCCATCAGACAGCTACTACCATTTCAAATCAAAATGGATCTCCT
TTTCACCAACAAGGACATTCACACTCTATGCATCAAAATAAAAGCTTTGTGGCACACCAT
GACTTTGCCTTATTTCAGGCCAATGAACAACAAACACAGTGTACTTCACTACGCTCACAA
CAAAACAGAAATAATCTCAACCCAGGGCAGAATTCTCTTAGCCAGTCTAAAAATTTTATG
AATGTTTCTGGTCCACATAGAGTCAATGTTAACCACCCACCACAGATGACTAATGCATCT
AATTCACAACAGTCTATTTCAATGCAGCAATTTTCTCAAACGTCAAATCCTTCAGCACAC
TTCCACAAGTGTAGCAGTCATCAAGAAGGAAATTTTAATGGACCTTCCCCAAATATGACT
TCTTGTTCTGTCAGTAATTCACAGCAATTTTCTTCACATTATTCCTTTTCCAGTAATCAT
ATATCACCAAACAGTCTACTTCAGTCCTCTGCAGTTCTTGCATCTAATCATACAAATCAG
ACTTTATCTGATTTTACTGGAAGTAATTCCTTTTCACCTCATAGAGGAATCAAGCAAGAA
TCTACTCAGCATATCCTAAACCCCAATACATCATTGAATTCAAATAATTTCCAAATATTG
CATTCATCACATCCTCAGGGTAATTATAGCAATTCAAAATTATCTCCTGTGCACATGAAC
TTCCCAGATCCTGTTGACTCAGGAACTCAAATGGGCCATTTCAATGATCATGTAGAAACT
AATGGCTTTTCATCTTTAGAAGAGAATTTACTTCATCAAGTGGAATCTCAAACTGAGCCA
TTCACAGGACTTGACCCCGAGGACCTCCTTCAGGAGGGTCTTCTTCCTCACTTTGATGAG
TCAACATTCGGACAAGATAATTCAAGTCACATTTTAGATCATGACCTTGATCGGCAGTTT
ACTTCGCATCTGGTAACACGGCCTTCTGATATGGCTCAGACTCAGTTGCAAAGTCAGGCT
CGGAGTTGGCATTCATCATTTTCTAATCATCAGCATTTACATGACAGAAATCACCTATGT
TTACAGCGACAGCCTCCATCTTCCAAGAAGAGCGATGGTTCTGGGACATATACTAAGTTG
CAGAATACCCAGGTGAGGGTCATGTCTGAGAAGAAGCAGAGAAAAAAGGTGGAATCAGAA
AGCAAGCAAGAAAAGGCTAATCGTATAATATCAGAGGCCATAGCAAAAGCAAAGGAGCGT
GGGGAACGCAATATTCCACGAGTAATGAGCCCTGAAAACTTTCCTACTGCTTCAGTTGAA
GGAAAAGAGGAAAAGAAAGGTAGAAGGATGAAATCCAAGCCAAAGGACAAAGACAGCAAA
AAAACAAAAACATGTTCTAAGTTAAAAGAGAAGACAAAAATTGGCAAACTCATTATTACA
TTGGGTAAGAAACAAAAAAGAAAGAATGAGTCTTCAGATGAAATATCTGATGCAGAACAG
ATGCCACAGCATACATTAAAAGATCAAGACTCTCAAAAAAGAAGATCAAATCGACAAATT
AAAAGAAAAAAATACGCAGAAGATATAGAAGGGAAGCAATCTGAAGAAGAGGTTAAAGGT
TCTATGAAAATAAAAAAGAATTCAGCTCCTTTACCTGGTGAACAGCCTTTACAATTGTTT
GTGGAGAATCCGAGTGAAGAAGATGCTGCAATTGTAGACAAAATTCTATCTTCTAGAACC
GTAAAAAAGGAAATATCACCTGGAGTGATGATTGATACAGAAGAATTTTTTGTAAAATAC
AAGAATTACTCCTATCTTCACTGTGAGTGGGCCACAGAAGAGCAGCTTTTGAAAGATAAA
AGGATCCAGCAGAAAATCAAACGATTCAAATTGAGACAAGCACAAAGAGCACATTTTTTT
GCAGACATGGAAGAAGAACCATTTAACCCAGACTACGTTGAAGTAGACAGAGTATTAGAA
GTCTCTTTTTGTGAAGATAAGGATACTGGTGAGCCTGTTATTTACTACTTAGTAAAATGG
TGCTCATTGCCATATGAAGATAGTACTTGGGAACTAAAAGAAGATGTAGATCTTGCAAAA
ATAGAAGAGTTTGAACAACTGCAAGCTTCAAGGCCTGACACAAGACGTTTGGACCGTCCT
CCTTCTAATATTTGGAAGAAAATAGATCAATCCAGGGACTATAAAAATGGCAATCAACTC
AGGGAATATCAACTGGAAGGACTCAACTGGCTCTTGTTCAATTGGTACAATAGACGAAAC
TGCATCTTAGCAGATGAAATGGGTCTTGGCAAAACTATTCAATCAATTACATTCCTCTAT
GAAATCCTTCTGACTGGTATAAGAGGACCTTTCCTGATTATTGCTCCACTTTCTACTATT
GCAAACTGGGAGAGAGAATTTCGTACGTGGACTGATATTAACGTTGTGGTTTATCATGGG
AGCCTGATTAGCAGACAAATGATACAGCAATACGAGATGTACTTCAGGGATTCACAGGGG
CGTATCATTCGAGGAGCTTACAGATTCCAAGCCATCATCACCACTTTTGAAATGATTCTT
GGAGGCTGTGGAGAGCTTAATGCAATTGAATGGCGATGTGTGATTATTGATGAAGCACAT
AGGTTAAAAAATAAAAATTGTAAACTCTTAGAGGGCCTGAAACTCATGAATCTGGAACAC
AAGGTGCTTTTGACTGGCACCCCTCTCCAAAATACAGTTGAAGAACTATTTAGTCTTCTT
CACTTTCTTGAACCCTTAAGGTTTCCTTCTGAATCAACATTTATGCAAGAATTTGGGGAT
CTGAAAACAGAGGAACAGGTACAGAAACTTCAGGCTATCCTGAAACCAATGATGTTGAGA
CGATTAAAAGAAGATGTGGAAAAGAAGTTGGCACCTAAAGAAGAAACCATCATTGAAGTA
GAACTTACTAATATTCAAAAGAAATACTACCGGGCTATCTTGGAAAAGAACTTTTCTTTT
TTATCCAAAGGAGCAGGACAAACTAATGTACCTAACTTGGTCAATACCATGATGGAGCTC
AGGAAATGTTGTAATCATCCATATCTTATAAAAGGTGCTGAGGAGAAAATACTTGGAGAA
TTTAGAGATACTTACAATCCAGCTGCTTCTGATTTTCATCTTCAAGCAATGATCCAGTCT
GCTGGTAAATTGGTCCTTATTGATAAATTGCTTCCCAAAATGAAAGCCGGAGGTCATAAA
GTGCTCATCTTCTCTCAAATGGTTCGTTGCCTTGACATTCTGGAGGACTATCTCATACAT
AAAAGATACTTATATGAGCGAATTGATGGCAGAGTCAGAGGAAATCTTCGGCAAGCTGCT
ATAGATAGATTTAGTAAACCTGATTCAGATAGATTTGTTTTTCTCCTGTGTACCCGAGCT
GGTGGGTTGGGCATCAACTTAACTGCAGCTGATACATGTATAATTTTTGATTCTGATTGG
AATCCTCAGAATGATCTTCAGGCCCAAGCTCGTTGCCACAGAATTGGTCAGAACAAAGCA
GTTAAAGTCTACAGACTGGTAACTCGTAACTCATATGAGAGAGAGATGTTTGACCGAGCC
AGTTTGAAACTGGGCCTAGATAAAGCTGTGTTACAGAGCATGAGTGGAAGAGAAAGTAAT
GTTGGTGGTATTCAGCAGCTTTCCAAAAAGGAAATAGAAGATCTGCTTCGAAGAGGTGCT
TATGGTGCTATTATGGAGGAAGAAGATGAAGGCTCTAAATTCTGCGAAGAGGATATCGAT
CAGATTTTACTACGTCGTACAAAAACTATTACAATTGAATCAGAAGGACGTGGGTCAACA
TTTGCCAAGGCGAGTTTTGTGGCATCTGGAAACCGGACAGATATTTCTTTAGATGATCCC
AACTTCTGGCAAAAATGGGCTAAAAAGGCAGAAATAGATATAGAGGCCATCAGTGGCAGA
AACAGCTTGGTTATTGACACTCCAAGAATTAGGAAGCAAACAAGACCTTTTAGTGCCACA
AAAGATGAATTGGCTGAATTATCTGAAGCTGAAAGTGAAGGAGATGAAAAGCCCAAACTC
CGGAGACCCTGTGACCGTTCCAATGGCTATGGAAGAACTGAATGCTTTAGAGTTGAGAAA
AACCTGCTAGTTTATGGGTGGGGCCGATGGAGAGAGATTCTATCTCATGGCCGTTTCAAA
AGGCAGCTAAATGAACACGATGTAGAGATAATTTGCCGAGCTCTCTTAGCATATTGCCTT
GTTCACTACCGAGGAGATGAGAAGATTAAAGGTTTCATATGGGATCTCATTACTCCAACT
GAAGATGGACAGACACGAGAGCTACAGAATCATCTAGGCCTATCAGCTCCTGTACCCAGG
GGTCGAAAAGGGAAGAAAGTAAAAACTCAAACAAGCTCATTTGATATACAAAAAGCAGAA
TGGCTTCGAAAATATAATCCCGAGCAGCTCCTTCAAGATGAAGGCTACAAAAAACATATA
AAACACCACTGTAATAAGGTTTTGCTTCGTGTGAGAATGCTGTATTATCTAAAGCAAGAA
GTTATTGGAAATGAGTGTCAGAAAGTATTTGATGGAGTTGATGCAAGTGACATTGATGTT
TGGGTACCAGAACCAGACCACTCAGAAGTTCCTGCTGAGTGGTGGGATTTTGATGCTGAT
AAGTCACTCCTTATTGGAGTTTTTAAACATGGATATGAAAAATATAACACTATTCGAGCA
GACCCAGCATTATGCTTCTTGGAAAGAGTGGGAAAACCTGATGAGAAAGCAGTTGCTGCT
GAACAGAGAGCGAATGATTATATGGATGGGGATGTGGAAGATCCAGAATACAAACCTGCC
CCAGCCATCTTTAAAGATGATATAGAGGATGATGTTTCCTCACCAGGAGATCTTGTTATA
GCAGATGGAGATGGTCAACTGATGGAGGGTGATAAAGTATATTGGCCTACTCAATCAGCT
TTAACCACACGTTTGAGGCGTCTCATCACTGCATACCAGCGTACTAATAAAAACAGACAA
ATTCAGCAGATACAACCGACTTTCTCGGTGCCTACCAGTGTAATGCAGCCTATTTATGAG
GAAGCCACTCTTAATCCTAAAATGGCAGCCAAGATAGAAAGACAGCAAAGATGGACAAGA
AGAGAAGAAGCTGACTTTTATAGGGTTGTATCTACATTTGGAGTGGTTTTTGACCCTGAC
AGAGGCCAATTTGATTGGACAAAATTTAGAGCTATGGCTAGGCTACATAAGAAAACTGAT
GATAGTTTGGAAAAATATTTGTACGCATTCATGTCCATGTGTCGGAGGGTTTGTCGTCTT
CCTTCCAAAGAAGAATTGGTGGATCCAAATATTTTTATCCAGCCCATCACAGAAGAACGT
GCTTCTAGGACTTTGTATCGCATTGAACTTCTAAGGAAAGTACGGGAACAGGCCCTTCGA
CATCCACAGTTGTTTGAACGCTTGAAGCTTTGCCATCCAAATCCAGATTTACCAGTCTGG
TGGGAATGTGGCCCTCATGATAGGGATTTGCTTATTGGTGCTGCCAAACACGGGGTGAGC
CGAACAGACTATCACATTCTTCGTGATCCTGAACTCTCATTTATGGCAGCTCAGAGGAAC
TACAGTCAAAGTAAGATGGCTCATTCAAGGACTTCTACCCCACTTCTACAGCAATATCAA
GTAGCACTTTCTGCTTCTCCTCTTACCTCTCTACCTAGGCTCCTAGATGCTAAAGGTATT
ATTCTAGAGGAGATGAAAGTTAAAAGTGAAAACCTTAAAGAGGAGCCTCAGTCTTCTGAA
GAAGAATCTATGTCTTCTGTGGAAACCAGGACACTAATAAAATCTGAGCCTGTAAGTCCA
AAGAATGGTGTTTTACCACAGGCTACTGGAGACCAGAAATCTGGTGGAAAATGTGAAACA
GACAGACGCATGGTTGCAGCCAGAACAGAACCCCTAACTCCAAACCCAGCTTCTAAGAAA
CCAAGAGTCCACAAAAGGGGATCAGAATCTAGTTCTGATTCTGACTCAGATTCTGAGAGA
TCATCTTGTTCTTCCAGATCATCTTCTTCCTCATCATCCTCTTCTTGCTCCCACTCTCGA
TCAGGCTCTAGTTCTTCTTCATCTTCATCTTGTTCTTCAGCATCTTCTTCATCCTCTTCC
TCCACCTCTTCCTCCTCCTCCTCCTCTTCATCTTCATCAGAAGAAAGTGACAGTGATGAA
GAAGAAGCCCAAAAACGAGAAAGTACTACTCACATGAAAGCCTATGATGAAGAAAGCGTC
GCGTCACTGAGCACTACCCAGGATGAGACTCAGGATAGTTTTCAGATGAACAATGGGACA
CCAGAGTCTGCTTATATCTTACAAGGTGGATATATGCTGGCAGCCTCGTATTGGCCAAAG
GATCGTGTGATGATCAATAGGTTGGACAGTATTTGTCAAACAGTTCTGAAAGGAAAGTGG
CCTTCAGCTAGAAGAAGTTATGATGCTAACACAGTGGCTTCTTTCTATACCACAAAACTG
CTGGACAGCCCTGGAGCAGCTACAGAATACAGCGATCCCAGTGTACCCACTCCCCCAGGT
GCCGGTGTTAAAGAAGAACATGATCAGTCAACACAGATGTCAAAGGTGAAGAAGCATGTA
CGAGAAAAGGAGTTTACAGTGAAAATCAAAGACGAAGGTGGTTTGAAGTTGACATTTCAG
AAGCAAGGGCTTGCTCAGAAAAGACCATTTGATGGTGAAGACGGTGCTCTGGGGCAGCAG
CAGTACCTCACTCGGCTTCGAGAGCTTCAAAGTGCATCAGAGACCAGCCTCGTCAATTTC
CCAAAATCCATACCAGTATCAGGTACTTCCATTCAACCAACCCTTGGTGCCAATGGTGTG
ATATTAGACAACCAGCCTATAGTCAAAAAAAGGCGAGGAAGGAGGAAGAATGTAGAAGGT
GTTGACATCTTCTTTTTTAACAGAAATAAACCACCTAATCATGTTTCTTTAGGCTTAACC
TCCTCACAGATTTCCACAGGGATAAATCCAGCACTATCCTATACTCAACCTCAAGGAATT
CCTGATACAGAAAGTCCAGTTCCAGTTATTAATCTTAAAGATGGAACGAGACTTGCAGGA
GATGATGCACCAAAGAGAAAGGATTTGGAAAAATGGCTTAAGGAGCACCCGGGTTATGTG
GAAGATTTGGGAGCTTTTATTCCTAGAATGCAGCTTCATGAGGGAAGACCCAAACAAAAA
AGACACCGTTGCAGAAACCCCAATAAACTAGATGTGAATAGTCTCACTGGAGAAGAACGT
GTTCAACTGATTAACAGAAGAAATGCTAGAAAGGTTGGAGGTGCATTTGCTCCCCCTTTG
AAAGATTTATGTAGATTCCTAAAAGAAAATTCAGAATATGGAGTAGCTCCTGAATGGGGA
GATGTTGTTAAGCAATCTGGATTTCTTCCAGAAAGCATGTATGAACGTATTCTCACTGGT
CCCGTTGTGAGAGAGGAAGTAAGCAGGCGGGGGAGACGGCCTAAAAGTGGAATTGCAAAG
GCCACAGCAGCAGCAGCTGCTGCATCTGCCACCAGTGTTTCAGGCAATCCTTTGTTAGCC
AATGGACTACTTCCAGGTGTGGATCTCACAACTCTTCAGGCCTTACAACAAAACCTACAA
AACTTGCAGTCACTGCAAGTAACTGCTGGGTTGATGGGAATGCCTACCGGCCTTCCTTCT
GGAGGAGAAGCTAAAAACATGGCTGCTATGTTCCCCATGCTGCTGTCAGGAATGGCTGGA
TTACCAAATCTGTTGGGCATGGGAGGACTCCTGACAAAGCCTACGGAATCTGGGACAGAA
GACAAAAAGGGAAGTGACTCTAAGGAGTCAGAAGGAAAAACAGAAAGGACAGAGAGCCAA
AGTTCAGAGAATGGTGGAGAAAACTCTGTGTCAAGTTCTCCTTCCACATCCTCTACTGCT
GCATTAAATACAGCTGCAGCTGCCAACCCATTAGCTCTTAACCCACTATTACTATCTAAT
ATACTTTATCCAGGGATGCTTCTCACTCCAGGCCTTAATCTTCATATTCCAACTTTGTCC
CAGTCCAATACTTTTGATGTACAAAACAAAAACAGTGACTTAGGCTCGTCTAAGTCTGTA
GAAGTAAAAGAAGAAGATTCCAGAATTAAAGATCAGGAAGACAAAGGAGGAACTGAACCA
AGTCCTCTCAATGAAAACAGCACAGATGAGGGTTCAGAGAAAGCTGATGCTTCATCTGGA
TCTGATAGTACATCGTCGTCATCTGAGGATTCAGATTCTAGTAATGAAGAC
Protein sequence predicted based on nucleotide sequence
MTDPMMDFFDDANLFGETLEGLSDDAFVQPGPVSLVDELNLGAEFEPLHIDSLNHVQGTP THQKMTDFEQLNQFDSIKFHHVNQSFGSPAEHVLSPHSQFNCSPIHPQNQPNGLFPDVSD GSPMWGHQTATTISNQNGSPFHQQGHSHSMHQNKSFVAHHDFALFQANEQQTQCTSLRSQ QNRNNLNPGQNSLSQSKNFMNVSGPHRVNVNHPPQMTNASNSQQSISMQQFSQTSNPSAH FHKCSSHQEGNFNGPSPNMTSCSVSNSQQFSSHYSFSSNHISPNSLLQSSAVLASNHTNQ TLSDFTGSNSFSPHRGIKQESTQHILNPNTSLNSNNFQILHSSHPQGNYSNSKLSPVHMN FPDPVDSGTQMGHFNDHVETNGFSSLEENLLHQVESQTEPFTGLDPEDLLQEGLLPHFDE STFGQDNSSHILDHDLDRQFTSHLVTRPSDMAQTQLQSQARSWHSSFSNHQHLHDRNHLC LQRQPPSSKKSDGSGTYTKLQNTQVRVMSEKKQRKKVESESKQEKANRIISEAIAKAKER GERNIPRVMSPENFPTASVEGKEEKKGRRMKSKPKDKDSKKTKTCSKLKEKTKIGKLIIT LGKKQKRKNESSDEISDAEQMPQHTLKDQDSQKRRSNRQIKRKKYAEDIEGKQSEEEVKG SMKIKKNSAPLPGEQPLQLFVENPSEEDAAIVDKILSSRTVKKEISPGVMIDTEEFFVKY KNYSYLHCEWATEEQLLKDKRIQQKIKRFKLRQAQRAHFFADMEEEPFNPDYVEVDRVLE VSFCEDKDTGEPVIYYLVKWCSLPYEDSTWELKEDVDLAKIEEFEQLQASRPDTRRLDRP PSNIWKKIDQSRDYKNGNQLREYQLEGLNWLLFNWYNRRNCILADEMGLGKTIQSITFLY EILLTGIRGPFLIIAPLSTIANWEREFRTWTDINVVVYHGSLISRQMIQQYEMYFRDSQG RIIRGAYRFQAIITTFEMILGGCGELNAIEWRCVIIDEAHRLKNKNCKLLEGLKLMNLEH KVLLTGTPLQNTVEELFSLLHFLEPLRFPSESTFMQEFGDLKTEEQVQKLQAILKPMMLR RLKEDVEKKLAPKEETIIEVELTNIQKKYYRAILEKNFSFLSKGAGQTNVPNLVNTMMEL RKCCNHPYLIKGAEEKILGEFRDTYNPAASDFHLQAMIQSAGKLVLIDKLLPKMKAGGHK VLIFSQMVRCLDILEDYLIHKRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRA GGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQNKAVKVYRLVTRNSYEREMFDRA SLKLGLDKAVLQSMSGRESNVGGIQQLSKKEIEDLLRRGAYGAIMEEEDEGSKFCEEDID QILLRRTKTITIESEGRGSTFAKASFVASGNRTDISLDDPNFWQKWAKKAEIDIEAISGR NSLVIDTPRIRKQTRPFSATKDELAELSEAESEGDEKPKLRRPCDRSNGYGRTECFRVEK NLLVYGWGRWREILSHGRFKRQLNEHDVEIICRALLAYCLVHYRGDEKIKGFIWDLITPT EDGQTRELQNHLGLSAPVPRGRKGKKVKTQTSSFDIQKAEWLRKYNPEQLLQDEGYKKHI KHHCNKVLLRVRMLYYLKQEVIGNECQKVFDGVDASDIDVWVPEPDHSEVPAEWWDFDAD KSLLIGVFKHGYEKYNTIRADPALCFLERVGKPDEKAVAAEQRANDYMDGDVEDPEYKPA PAIFKDDIEDDVSSPGDLVIADGDGQLMEGDKVYWPTQSALTTRLRRLITAYQRTNKNRQ IQQIQPTFSVPTSVMQPIYEEATLNPKMAAKIERQQRWTRREEADFYRVVSTFGVVFDPD RGQFDWTKFRAMARLHKKTDDSLEKYLYAFMSMCRRVCRLPSKEELVDPNIFIQPITEER ASRTLYRIELLRKVREQALRHPQLFERLKLCHPNPDLPVWWECGPHDRDLLIGAAKHGVS RTDYHILRDPELSFMAAQRNYSQSKMAHSRTSTPLLQQYQVALSASPLTSLPRLLDAKGI ILEEMKVKSENLKEEPQSSEEESMSSVETRTLIKSEPVSPKNGVLPQATGDQKSGGKCET DRRMVAARTEPLTPNPASKKPRVHKRGSESSSDSDSDSERSSCSSRSSSSSSSSSCSHSR SGSSSSSSSSCSSASSSSSSSTSSSSSSSSSSSEESDSDEEEAQKRESTTHMKAYDEESV ASLSTTQDETQDSFQMNNGTPESAYILQGGYMLAASYWPKDRVMINRLDSICQTVLKGKW PSARRSYDANTVASFYTTKLLDSPGAATEYSDPSVPTPPGAGVKEEHDQSTQMSKVKKHV REKEFTVKIKDEGGLKLTFQKQGLAQKRPFDGEDGALGQQQYLTRLRELQSASETSLVNF PKSIPVSGTSIQPTLGANGVILDNQPIVKKRRGRRKNVEGVDIFFFNRNKPPNHVSLGLT SSQISTGINPALSYTQPQGIPDTESPVPVINLKDGTRLAGDDAPKRKDLEKWLKEHPGYV EDLGAFIPRMQLHEGRPKQKRHRCRNPNKLDVNSLTGEERVQLINRRNARKVGGAFAPPL KDLCRFLKENSEYGVAPEWGDVVKQSGFLPESMYERILTGPVVREEVSRRGRRPKSGIAK ATAAAAAASATSVSGNPLLANGLLPGVDLTTLQALQQNLQNLQSLQVTAGLMGMPTGLPS GGEAKNMAAMFPMLLSGMAGLPNLLGMGGLLTKPTESGTEDKKGSDSKESEGKTERTESQ SSENGGENSVSSSPSTSSTAALNTAAAANPLALNPLLLSNILYPGMLLTPGLNLHIPTLS QSNTFDVQNKNSDLGSSKSVEVKEEDSRIKDQEDKGGTEPSPLNENSTDEGSEKADASSG SDSTSSSSEDSDSSNED
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
