Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745914
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6747nts
Open reading frame: 1 to 6747
ATGGCCCACAACGCTGGAGCTGCGGCCGCGGCTGGCACACACAGCGCCAAGTCTGGGGGC
TCCGAGGCCGCCCTCAAGGAGGGCGGAAGTGCCGCCGCGTTATCTAGCTCCAGTTCGAGC
AGTGCGGCTGCCGCGGCGGCTAGCTCTTCCTCATCTAGTGGCCCGGGCTCAGCCATGGAG
ACAGGATTACTGCCCAACCACAAGCTGAAGACCGTCGGCGAGGCTCCCGCCGCACCTCCC
CACCAGCAGCACCACCACCATCATCACGCCCATCATCATCACCACCATGCTCACCATCTC
CATCACCATCACGCTCTCCAGCAACAATTGAACCAGTTTCAGCAGCAGCAACAACAACAG
CAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCATCCCATCAGCAATAACAATAGCTTG
GGCGGGGCAGGCGGGGGTGCTCCCCAACCCGGCCCAGACATGGAACAACCCCAGCACGGA
GGCGCTAAGGACAGTGCTGCAGGCGGCCAGGCCGACCCCCCCGGCCCTCCTCTCCTGAGC
AAGCCTGGAGACGAAGACGACGCACCCCCCAAGATGGGCGAGCCCGCTGGCGGTAGATAC
GAACACCCTGGCCTCGGCGCCCTGGGCACACAACAGCCGCCGGTGGCTGTCCCCGGAGGA
GGAGGCGGTCCCGCTGCCGTGCCCGAGTTCAACAACTACTACGGGAGCGCTGCTCCTGCA
AGCGGAGGCCCCGGGGGCAGAGCCGGTCCCTGCTTCGACCAACACGGCGGACAACAGAGC
CCCGGGATGGGGATGATGCACAGCGCTAGTGCTGCCGCCGCCGGAGCTCCTGGCAGCATG
GACCCCCTCCAGAACTCCCACGAGGGATACCCTAACAGCCAGTGCAACCACTACCCTGGA
TACAGCAGGCCTGGTGCTGGAGGTGGCGGGGGCGGCGGAGGCGGCGGTGGAGGAGGTTCA
GGAGGCGGAGGTGGAGGTGGTGGAGCTGGAGCAGGAGGTGCTGGAGCCGGAGCAGTGGCA
GCTGCTGCCGCTGCCGCAGCGGCTGCAGCAGGGGGAGGGGGAGGAGGGGGCTATGGGGGC
AGTTCAGCCGGGTATGGCGTTCTGTCTAGCCCCAGACAGCAAGGCGGTGGCATGATGATG
GGTCCCGGTGGCGGTGGGGCAGCTTCACTCTCTAAAGCGGCGGCCGGAAGCGCCGCGGGT
GGCTTCCAAAGATTCGCCGGCCAGAACCAGCACCCATCTGGAGCCACCCCGACCCTGAAC
CAGCTGCTTACCTCTCCCTCTCCCATGATGAGAAGCTATGGCGGCAGTTACCCCGAGTAC
AGCAGCCCCTCCGCTCCGCCCCCACCCCCTAGTCAGCCCCAAAGCCAAGCTGCAGCGGCT
GGTGCAGCCGCAGGAGGCCAACAGGCGGCAGCTGGCATGGGCCTGGGCAAGGACATGGGA
GCCCAGTATGCAGCAGCCAGCCCCGCGTGGGCCGCTGCTCAACAGAGGTCACACCCCGCG
ATGAGCCCTGGAACCCCCGGACCCACAATGGGCAGAAGCCAGGGCAGCCCCATGGATCCC
ATGGTCATGAAGCGCCCCCAGCTGTACGGAATGGGAAGCAACCCCCATTCTCAGCCTCAG
CAGAGCAGCCCTTACCCTGGCGGCTCCTACGGCCCCCCAGGACCACAAAGATACCCCATC
GGCATACAGGGCAGAACACCCGGCGCCATGGCCGGAATGCAGTATCCTCAACAGCAGGAC
AGCGGAGACGCCACCTGGAAAGAGACATTCTGGCTGATGCCCCCTCAGTACGGCCAGCAA
GGAGTGAGCGGCTACTGCCAGCAGGGTCAGCAGCCTTACTACAGCCAGCAACCTCAGCCC
CCACACCTCCCTCCTCAGGCTCAATACCTGCCCTCTCAGAGCCAGCAGAGATACCAGCCA
CAGCAAGATATGTCTCAGGAGGGCTACGGCACCAGATCTCAACCACCTCTGGCCCCCGGC
AAACCCAACCATGAGGACCTGAACTTGATCCAACAAGAGCGACCCTCCAGCCTGCCCGAC
CTGTCTGGCTCCATCGATGACCTCCCTACAGGCACCGAGGCTACCCTTAGCTCCGCGGTG
AGCGCATCCGGCTCTACCTCGAGCCAAGGCGATCAGAGCAATCCCGCCCAGTCCCCCTTT
TCCCCCCACGCATCCCCCCATCTCTCCTCGATCCCGGGCGGCCCTTCCCCCTCACCCGTA
GGCAGCCCTGTAGGAAGCAATCAGTCTCGGTCAGGCCCTATCTCCCCCGCTAGCATCCCC
GGGTCCCAGATGCCTCCACAACCACCAGGGTCACAGTCCGAGAGCTCTAGTCACCCCGCC
CTGAGCCAATCCCCTATGCCCCAGGAAAGAGGCTTCATGGCAGGCACACAGAGAAACCCT
CAGATGGCCCAATACGGACCCCAACAGACCGGCCCCAGCATGTCTCCTCACCCTAGCCCC
GGCGGGCAGATGCATGCCGGAATCTCCTCCTTTCAACAGTCCAACTCATCCGGAACCTAC
GGGCCACAGATGAGCCAGTACGGACCTCAGGGCAACTACTCCAGACCTCCCGCCTACTCT
GGCGTGCCTTCTGCCTCCTACTCCGGTCCCGGGCCTGGTATGGGTATCAGCGCAAACAAC
CAGATGCACGGCCAAGGACCATCCCAGCCCTGCGGCGCCGTCCCCCTCGGCCGCATGCCC
AGCGCCGGGATGCAAAACAGACCATTCCCCGGAAATATGTCAAGCATGACCCCAAGTTCT
CCTGGGATGTCGCAACAGGGAGGGCCTGGAATGGGGCCGCCGATGCCCACAGTCAACAGA
AAGGCCCAAGAGGCGGCTGCGGCCGTGATGCAGGCTGCAGCCAACTCCGCACAGAGCCGA
CAGGGCTCCTTCCCGGGCATGAATCAGAGCGGCCTCATGGCTAGCAGCAGTCCCTACTCC
CAGCCTATGAACAACTCTAGTTCCCTGATGAACACACAGGCTCCCCCCTACTCAATGGCG
CCCGCCATGGTTAACTCCAGCGCAGCTTCTGTGGGTCTTGCCGATATGATGAGTCCTGGT
GAGAGCAAGTTGCCCCTCCCTCTGAAGGCCGACGGCAAAGAAGAGGGCACCCCACAGCCC
GAAAGCAAGAGCAAGAAGTCCTCTTCTAGCACTACCACCGGAGAGAAGATCACAAAGGTG
TACGAGCTGGGCAACGAGCCCGAGCGGAAGCTGTGGGTTGACAGATACCTGACATTCATG
GAGGAGAGGGGCTCTCCTGTGTCCTCACTGCCTGCCGTGGGCAAGAAGCCCCTCGACCTG
TTCAGACTTTACGTGTGCGTCAAGGAGATTGGCGGCTTGGCCCAGGTCAACAAAAACAAG
AAGTGGAGGGAACTGGCCACCAACCTGAACGTGGGCACCTCTAGCAGCGCCGCCAGCAGT
CTGAAGAAGCAGTACATCCAGTACCTGTTCGCCTTCGAGTGCAAGATCGAGAGAGGCGAA
GAACCACCCCCCGAGGTGTTCAGCACAGGCGACACAAAAAAGCAGCCCAAACTGCAACCT
CCTTCTCCCGCCAACAGCGGTAGCCTGCAAGGCCCACAGACCCCCCAGAGCACCGGCAGC
AACTCTATGGCCGAGGTGCCAGGCGACTTGAAGCCGCCCACCCCGGCCTCCACCCCTCAC
GGCCAGATGACCCCCATGCAGGGCGGCAGATCTTCAACCATCAGCGTTCACGACCCCTTC
AGCGACGTGAGCGACAGCAGCTTCCCCAAGAGAAACTCCATGACTCCCAACGCCCCCTAC
CAACAGGGCATGTCTATGCCCGACGTCATGGGCCGTATGCCATACGAGCCCAACAAGGAC
CCATTCGGGGGCATGAGAAAGGTGCCCGGCTCAAGCGAGCCCTTTATGACACAGGGACAG
ATGCCCAATAGCAGCATGCAAGACATGTACAACCAGTCACCCAGCGGGGCCATGAGCAAC
TTGGGCATGGGACAACGGCAACAGTTCCCCTACGGGGCCAGCTACGACCGGAGGCACGAG
CCTTACGGTCAACAGTATCCGGGCCAGGGCCCCCCCTCCGGACAGCCCCCGTACGGAGGC
CACCAGCCCGGCCTTTACCCCCAGCAGCCGAACTACAAGCGGCACATGGACGGCATGTAC
GGGCCTCCCGCTAAGAGGCATGAAGGGGACATGTATAACATGCAGTACAGCTCCCAACAA
CAGGAGATGTACAATCAGTATGGAGGCTCTTACTCCGGCCCCGACAGAAGACCCATCCAG
GGCCAGTACCCCTACCCCTATAGCAGAGAGAGGATGCAGGGGCCGGGCCAAATCCAGACA
CACGGAATCCCACCGCAAATGATGGGAGGACCCCTGCAGTCCAGCTCTAGCGAAGGCCCT
CAGCAAAACATGTGGGCTGCCAGAAACGACATGCCTTATCCCTACCAGAACAGGCAGGGC
CCAGGCGGACCTACCCAGGCACCCCCATATCCCGGCATGAACAGAACCGACGACATGATG
GTCCCTGACCAGAGAATCAACCACGAGTCCCAGTGGCCATCTCACGTCTCCCAGAGGCAA
CCCTACATGAGTTCCAGCGCCTCTATGCAGCCCATCACACGCCCACCACAGCCTTCCTAC
CAAACACCACCCTCTCTGCCAAACCACATCTCCCGTGCACCCAGCCCAGCCTCCTTCCAG
AGGTCCCTGGAGAACCGGATGTCACCTAGCAAAAGCCCTTTCCTGCCGAGCATGAAGATG
CAGAAAGTCATGCCCACCGTGCCCACTAGCCAGGTGACAGGGCCACCACCCCAACCGCCC
CCCATTAGAAGGGAGATTACCTTTCCCCCTGGCTCCGTCGAGGCCTCTCAGCCCGTGTTG
AAGCAGAGGAGAAAGATCACCTCTAAAGACATCGTGACACCCGAGGCTTGGAGAGTCATG
ATGTCCCTCAAGTCCGGTCTGCTCGCCGAGAGCACCTGGGCTTTGGACACCATCAACATC
CTTCTCTACGACGATAGCACCGTGGCCACCTTCAACCTGTCCCAGCTCAGCGGATTCCTG
GAGCTCTTAGTGGAGTACTTCAGAAAGTGCCTGATCGACATCTTCGGAATCCTGATGGAG
TACGAGGTCGGCGACCCCAGCCAAAAGGCTCTGGATCACAACGCCGCTAGAAAGGACGAC
AGCCAGAGTCTAGCTGACGACTCAGGCAAGGAAGAGGAGGATGCCGAGTGCATTGACGAC
GACGAGGAAGATGAAGAGGATGAGGAGGAAGACAGCGAGAAGACCGAGAGCGACGAGAAG
TCCAGCATCGCCCTGACTGCCCCTGACGCCGCAGCAGACCCAAAGGAGAAGCCCAAGCAG
GCAAGCAAGTTCGACAAGCTGCCTATCAAGATCGTTAAGAAGAACAACCTCTTCGTCGTG
GACCGCAGCGACAAACTGGGCAGAGTGCAGGAGTTTAACAGCGGCCTTCTTCACTGGCAG
TTGGGCGGTGGTGACACCACCGAGCACATCCAGACCCACTTCGAGAGCAAAATGGAGATC
CCTCCCAGAAGGCGACCTCCCCCCCCTTTGAGCTCTGCAGGCAGAAAGAAAGAGCAGGAA
GGTAAAGGAGATAGCGAAGAGCAGCAGGAGAAGAGCATCATCGCCACAATCGACGACGTG
CTGAGCGCCCGGCCAGGGGCACTGCCCGAGGACGCCAACCCTGGGCCTCAGACCGAAAGC
TCTAAGTTCCCTTTCGGAATTCAGCAAGCCAAGTCCCACAGGAACATCAAGCTGCTTGAG
GACGAACCCAGAAGCCGGGACGAAACCCCCTTGTGTACCATCGCACACTGGCAAGACTCC
CTGGCCAAGAGATGCATTTGCGTGAGCAACATCGTGCGCTCCCTGTCCTTTGTCCCTGGC
AACGACGCCGAGATGTCTAAGCACCCAGGCCTCGTGCTGATCCTGGGAAAGCTCATTCTC
CTTCACCACGAGCACCCTGAGAGGAAGCGGGCCCCTCAGACATACGAGAAGGAAGAAGAC
GAGGACAAGGGCGTGGCCTGCTCTAAGGACGAATGGTGGTGGGACTGCCTGGAGGTCCTG
AGAGACAATACCCTGGTCACCTTGGCCAACATCAGCGGGCAACTGGACCTGAGCGCTTAC
ACCGAGTCCATCTGCTTGCCAATTCTGGACGGCCTGTTACACTGGATGGTGTGCCCTAGC
GCAGAGGCTCAGGATCCTTTCCCAACAGTCGGCCCCAACTCTGTGCTGTCTCCCCAGAGA
CTGGTGCTTGAGACACTGTGCAAGCTGAGCATCCAGGACAACAACGTGGACCTTATCCTG
GCCACACCCCCGTTCTCCCGACAGGAGAAATTCTACGCCACACTCGTGAGATACGTGGGC
GACAGAAAAAACCCCGTCTGCCGAGAGATGTCCATGGCACTCCTGTCCAACCTTGCACAA
GGAGACGCTCTTGCCGCCAGAGCCATCGCTGTACAGAAGGGCAGCATCGGCAACCTGATC
AGCTTCCTGGAAGACGGCGTGACAATGGCCCAGTACCAGCAAAGCCAGCACAATCTCATG
CACATGCAGCCTCCGCCCCTGGAGCCTCCTAGCGTTGACATGATGTGCCGGGCCGCCAAG
GCATTGCTTGCTATGGCCAGAGTGGACGAGAACAGATCCGAGTTCCTTCTGCACGAGGGC
AGATTACTGGACATCAGCATTTCCGCTGTGTTGAACAGCCTCGTGGCCAGCGTGATCTGC
GACGTACTGTTCCAGATCGGACAGTTG
Protein sequence predicted based on nucleotide sequence
MAHNAGAAAAAGTHSAKSGGSEAALKEGGSAAALSSSSSSSAAAAAASSSSSSGPGSAME TGLLPNHKLKTVGEAPAAPPHQQHHHHHHAHHHHHHAHHLHHHHALQQQLNQFQQQQQQQ QQQQQQQQQQQHPISNNNSLGGAGGGAPQPGPDMEQPQHGGAKDSAAGGQADPPGPPLLS KPGDEDDAPPKMGEPAGGRYEHPGLGALGTQQPPVAVPGGGGGPAAVPEFNNYYGSAAPA SGGPGGRAGPCFDQHGGQQSPGMGMMHSASAAAAGAPGSMDPLQNSHEGYPNSQCNHYPG YSRPGAGGGGGGGGGGGGGSGGGGGGGGAGAGGAGAGAVAAAAAAAAAAAGGGGGGGYGG SSAGYGVLSSPRQQGGGMMMGPGGGGAASLSKAAAGSAAGGFQRFAGQNQHPSGATPTLN QLLTSPSPMMRSYGGSYPEYSSPSAPPPPPSQPQSQAAAAGAAAGGQQAAAGMGLGKDMG AQYAAASPAWAAAQQRSHPAMSPGTPGPTMGRSQGSPMDPMVMKRPQLYGMGSNPHSQPQ QSSPYPGGSYGPPGPQRYPIGIQGRTPGAMAGMQYPQQQDSGDATWKETFWLMPPQYGQQ GVSGYCQQGQQPYYSQQPQPPHLPPQAQYLPSQSQQRYQPQQDMSQEGYGTRSQPPLAPG KPNHEDLNLIQQERPSSLPDLSGSIDDLPTGTEATLSSAVSASGSTSSQGDQSNPAQSPF SPHASPHLSSIPGGPSPSPVGSPVGSNQSRSGPISPASIPGSQMPPQPPGSQSESSSHPA LSQSPMPQERGFMAGTQRNPQMAQYGPQQTGPSMSPHPSPGGQMHAGISSFQQSNSSGTY GPQMSQYGPQGNYSRPPAYSGVPSASYSGPGPGMGISANNQMHGQGPSQPCGAVPLGRMP SAGMQNRPFPGNMSSMTPSSPGMSQQGGPGMGPPMPTVNRKAQEAAAAVMQAAANSAQSR QGSFPGMNQSGLMASSSPYSQPMNNSSSLMNTQAPPYSMAPAMVNSSAASVGLADMMSPG ESKLPLPLKADGKEEGTPQPESKSKKSSSSTTTGEKITKVYELGNEPERKLWVDRYLTFM EERGSPVSSLPAVGKKPLDLFRLYVCVKEIGGLAQVNKNKKWRELATNLNVGTSSSAASS LKKQYIQYLFAFECKIERGEEPPPEVFSTGDTKKQPKLQPPSPANSGSLQGPQTPQSTGS NSMAEVPGDLKPPTPASTPHGQMTPMQGGRSSTISVHDPFSDVSDSSFPKRNSMTPNAPY QQGMSMPDVMGRMPYEPNKDPFGGMRKVPGSSEPFMTQGQMPNSSMQDMYNQSPSGAMSN LGMGQRQQFPYGASYDRRHEPYGQQYPGQGPPSGQPPYGGHQPGLYPQQPNYKRHMDGMY GPPAKRHEGDMYNMQYSSQQQEMYNQYGGSYSGPDRRPIQGQYPYPYSRERMQGPGQIQT HGIPPQMMGGPLQSSSSEGPQQNMWAARNDMPYPYQNRQGPGGPTQAPPYPGMNRTDDMM VPDQRINHESQWPSHVSQRQPYMSSSASMQPITRPPQPSYQTPPSLPNHISRAPSPASFQ RSLENRMSPSKSPFLPSMKMQKVMPTVPTSQVTGPPPQPPPIRREITFPPGSVEASQPVL KQRRKITSKDIVTPEAWRVMMSLKSGLLAESTWALDTINILLYDDSTVATFNLSQLSGFL ELLVEYFRKCLIDIFGILMEYEVGDPSQKALDHNAARKDDSQSLADDSGKEEEDAECIDD DEEDEEDEEEDSEKTESDEKSSIALTAPDAAADPKEKPKQASKFDKLPIKIVKKNNLFVV DRSDKLGRVQEFNSGLLHWQLGGGDTTEHIQTHFESKMEIPPRRRPPPPLSSAGRKKEQE GKGDSEEQQEKSIIATIDDVLSARPGALPEDANPGPQTESSKFPFGIQQAKSHRNIKLLE DEPRSRDETPLCTIAHWQDSLAKRCICVSNIVRSLSFVPGNDAEMSKHPGLVLILGKLIL LHHEHPERKRAPQTYEKEEDEDKGVACSKDEWWWDCLEVLRDNTLVTLANISGQLDLSAY TESICLPILDGLLHWMVCPSAEAQDPFPTVGPNSVLSPQRLVLETLCKLSIQDNNVDLIL ATPPFSRQEKFYATLVRYVGDRKNPVCREMSMALLSNLAQGDALAARAIAVQKGSIGNLI SFLEDGVTMAQYQQSQHNLMHMQPPPLEPPSVDMMCRAAKALLAMARVDENRSEFLLHEG RLLDISISAVLNSLVASVICDVLFQIGQL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
