Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00745978
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6465nts
Open reading frame: 1 to 6465
ATGGAATTGCAGAGGACGTCTAGTATCTCCGGGCCGCTGTCGCCGGCGTACACGGGGCAG
GTGCCTTACAACTACAACCAGCTGGAAGGCAGATTCAAGCAGCTGCAAGATGAGCGTGAA
GCCGTGCAGAAGAAGACCTTCACCAAGTGGGTCAATTCCCACCTTGCCCGTGTGTCCTGC
CGGATCACAGACCTGTACACTGACCTTCGAGATGGACGGATGCTCATCAAGCTGCTGGAG
GTCCTCTCTGGAGAGAGGCTGCCTAAACCCACCAAGGGACGAATGCGCATCCACTGCTTA
GAGAATGTGGACAAGGCCCTTCAGTTCCTGAAGGAGCAGAGAGTCCATCTTGAGAACATG
GGGTCCCATGACATCGTGGATGGAAACCACCGGCTGACCCTTGGCCTCATCTGGACCATC
ATCCTGCGCTTCCAGATCCAGGATATCAGTGTGGAAACTGAAGACAACAAAGAGAAGAAA
TCTGCCAAGGATGCATTGCTGTTGTGGTGCCAGATGAAGACAGCTGGGTACCCCAATGTC
AACATTCACAATTTCACCACTAGCTGGAGGGACGGCATGGCCTTCAATGCACTGATACAC
AAACACCGGCCTGACCTGATAGATTTTGACAAACTAAAGAAATCTAACGCACACTACAAC
CTGCAGAATGCATTTAATCTGGCAGAACAGCACCTCGGCCTCACTAAACTGTTGGACCCC
GAAGACATCAGCGTGGACCATCCTGATGAGAAGTCCATAATCACTTATGTGGTGACTTAT
TACCACTACTTCTCTAAGATGAAGGCCTTAGCTGTTGAAGGAAAACGAATTGGAAAGGTG
CTTGACAATGCTATTGAAACAGAAAAAATGATTGAAAAGTATGAATCACTTGCCTCTGAC
CTTCTGGAATGGATTGAACAAACCATCATCATTCTGAACAATCGCAAATTTGCCAATTCA
CTGGTCGGGGTTCAACAGCAGCTTCAGGCATTCAACACTTACCGCACTGTGGAGAAACCA
CCCAAATTTACTGAGAAGGGGAACTTGGAAGTGCTGCTCTTCACCATTCAGAGCAAGATG
AGGGCCAACAACCAGAAGGTCTACATGCCCCGGGAGGGGAAGCTCATCTCTGACATCAAC
AAGGCCTGGGAAAGACTGGAAAAAGCGGAACACGAAAGAGAACTGGCTTTGCGGAATGAG
CTCATAAGACAGGAGAAACTGGAACAGCTCGCCCGCAGATTTGATCGCAAGGCAGCTATG
AGGGAGACTTGGCTGAGCGAAAACCAGCGTCTGGTGTCTCAGGACAACTTTGGGTTTGAC
CTTCCTGCAGTTGAGGCCGCCACAAAAAAGCACGAGGCCATTGAGACAGACATTGCCGCA
TACGAGGAGCGTGTGCAGGCTGTGGTAGCCGTGGCCAGGGAGCTCGAGGCCGAGAATTAC
CACGACATCAAGCGCATCACAGCGAGGAAGGACAATGTCATCCGGCTCTGGGAATACCTA
CTGGAACTGCTCAGGGCCCGGAGACAGCGGCTCGAGATGAACCTGGGGCTGCAGAAGATA
TTCCAGGAAATGCTCTACATTATGGACTGGATGGATGAAATGAAGGTGCTAGTATTGTCT
CAAGACTATGGCAAACACTTACTTGGTGTGGAAGACCTGTTACAGAAGCACACCCTGGTT
GAAGCAGACATTGGCATCCAGGCAGAGCGGGTGAGAGGTGTCAATGCCTCCGCCCAGAAG
TTCGCAACAGACGGGGAAGGTTACAAGCCCTGTGACCCCCAGGTGATCCGAGACCGCGTG
GCCCACATGGAGTTCTGTTATCAAGAGCTTTGCCAGCTGGCGGCTGAGCGCAGGGCCCGT
CTGGAAGAGTCCCGCCGCCTCTGGAAGTTCTTCTGGGAGATGGCAGAAGAGGAAGGCTGG
ATACGGGAGAAGGAGAAGATCCTGTCCTCGGACGATTACGGGAAAGACCTGACCAGCGTC
ATGCGCCTGCTCAGCAAGCACCGGGCGTTCGAGGACGAGATGAGCGGCCGCAGTGGCCAC
TTTGAGCAGGCCATCAAGGAAGGCGAAGACATGATCGCGGAGGAGCACTTCGGGTCGGAG
AAGATCCGTGAGAGGATCATTTACATCCGGGAGCAGTGGGCCAACCTAGAGCAGCTCTCG
GCCATTCGGAAGAAGCGCCTGGAGGAGGCCTCCCTGCTGCACCAGTTCCAGGCAGATGCT
GATGACATTGATGCCTGGATGCTGGACATCCTCAAGATTGTCTCCAGCAGCGACGTGGGC
CACGATGAGTATTCCACACAGTCTCTGGTCAAGAAACACAAGGACGTGGCGGAAGAGATC
GCCAATTACAGGCCCACCCTTGACACGCTGCACGAACAAGCCAGCGCCCTCCCCCAGGAG
CATGCCGAGTCTCCAGACGTGAGGGGCAGGCTGTCGGGCATCGAGGAGCGGTATAAGGAG
GTGGCAGAGCTGACGCGGCTGCGGAAGCAGGCACTCCAGGACACTCTGGCCCTGTACAAG
ATGTTCAGCGAGGCTGATGCCTGTGAGCTCTGGATCGACGAGAAGGAGCAGTGGCTCAAC
AACATGCAGATCCCAGAGAAGCTGGAGGATCTGGAGGTCATCCAGCACAGATTTGAGAGC
CTAGAACCAGAAATGAACAACCAGGCTTCCCGGGTTGCAGTGGTGAACCAGATTGCACGC
CAGCTGATGCACAGCGGCCACCCAAGTGAGAAGGAAATCAAAGCCCAGCAGGACAAACTC
AACACAAGGTGGAGCCAGTTCAGAGAACTGGTTGACAGGAAGAAGGATGCCCTCCTGTCT
GCCCTGAGCATCCAGAACTACCACCTCGAGTGCAATGAAACCAAATCCTGGATTCGGGAA
AAGACCAAGGTCATCGAGTCCACCCAGGACCTGGGCAATGACCTGGCTGGCGTCATGGCC
CTGCAGCGCAAGCTGACCGGCATGGAGCGGGACTTGGTGGCCATTGAGGCAAAGCTGAGT
GACCTGCAGAAGGAGGCGGAGAAGCTGGAGTCCGAGCACCCCGACCAGGCCCAGGCCATC
CTGTCTCGGCTGGCCGAGATCAGCGACGTGTGGGAGGAGATGAAGACCACCCTGAAAAAC
CGAGAGGCCTCCCTGGGAGAGGCCAGCAAGCTGCAGCAGTTCCTACGGGACTTGGACGAC
TTCCAGTCCTGGCTCTCTAGGACCCAGACAGCGATCGCCTCGGAGGACATGCCAAACACC
CTGACCGAGGCTGAGAAGCTGCTCACGCAGCACGAGAACATCAAGAACGAGATCGACAAC
TACGAGGAGGACTACCAGAAGATGAGGGACATGGGCGAGATGGTCACCCAGGGGCAGACC
GATGCCCAGTACATGTTTCTGCGGCAGCGGCTGCAGGCCCTGGACACTGGATGGAACGAG
CTCCACAAGATGTGGGAGAATAGACAAAATCTCCTATCCCAGTCACATGCCTACCAGCAG
TTCCTCAGAGACACGAAGCAAGCCGAAGCCTTTCTTAACAACCAGGAGTATGTTCTGGCT
CACACTGAAATGCCTACCACCTTGGAAGGAGCTGAAGCAGCAATTAAAAAGCAAGAGGAC
TTCATGACCACCATGGACGCCAATGAGGAGAAGATCAATGCTGTGGTGGAGACTGGCCGG
AGGCTGGTGAGCGATGGGAACATCAACTCAGATCGCATCCAGGAGAAGGTGGACTCTATT
GATGACAGACATAGGAAGAATCGTGAGACAGCCAGTGAACTTTTGATGAGGTTGAAGGAC
AACAGGGATCTACAGAAATTCCTGCAAGATTGTCAAGAGCTGTCTCTCTGGATCAATGAG
AAGATGCTCACAGCCCAGGACATGTCTTACGATGAAGCCAGAAATCTGCACAGTAAATGG
TTGAAGCATCAAGCATTTATGGCAGAACTTGCATCCAACAAAGAATGGCTTGACAAAATC
GAGAAGGAAGGAATGCAGCTCATTTCAGAAAAGCCTGAGACGGAAGCTGTGGTGAAGGAG
AAACTCACTGGTTTACATAAAATGTGGGAAGTCCTTGAATCCACTACCCAGACAAAGGCC
CAGCGGCTCTTTGATGCAAACAAGGCCGAACTTTTCACCCAGAGCTGTGCAGATCTAGAC
AAATGGCTGCACGGCCTGGAGAGTCAGATTCAGTCTGATGACTATGGCAAAGACCTGACC
AGTGTCAATATCCTGCTGAAAAAGCAACAGATGCTGGAGAATCAGATGGAAGTGCGGAAG
AAGGAGATCGAAGAGCTCCAAAGCCAAGCCCAGGCCCTGAGTCAGGAAGGGAAGAGCACC
GACGAGGTAGACAGCAAGCGCCTCACCGTGCAGACCAAGTTCATGGAGTTGCTGGAGCCC
TTGAACGAGAGGAAGCATAACCTGCTGGCCTCCAAAGAGATCCATCAGTTCAACAGGGAT
GTGGAGGACGAGATCTTGTGGGTTGGAGAGAGGATGCCTTTGGCAACTTCCACGGATCAT
GGCCACAACCTCCAGACTGTGCAGCTGTTAATAAAGAAAAATCAGACCCTCCAGAAAGAA
ATCCAGGGGCACCAGCCTCGCATTGACGACATCTTTGAGAGGAGCCAAAACATCGTCACT
GACAGCAGCAGCCTCAGCGCTGAGGCCATCAGACAGAGGCTTGCCGACCTGAAGCAGCTG
TGGGGTCTCCTCATTGAGGAGACAGAGAAACGCCACAGGCGGCTGGAGGAGGCGCACAGG
GCCCAGCAGTACTACTTTGACGCTGCTGAGGCCGAAGCCTGGATGAGCGAGCAGGAGCTG
TACATGATGTCAGAGGAGAAGGCCAAGGATGAGCAGAGTGCTGTCTCCATGTTGAAGAAG
CACCAGATCTTAGAACAAGCTGTGGAGGACTATGCAGAGACCGTGCATCAGCTCTCCAAG
ACCAGCCGGGCCCTGGTGGCCGACAGCCATCCTGAAAGTGAGCGCATTAGCATGCGGCAG
TCCAAAGTGGATAAACTGTACGCTGGTCTGAAAGACCTTGCTGAAGAGAGAAGAGGCAAG
CTGGATGAGAGACACAGGTTATTCCAGCTCAACCGGGAGGTGGACGACCTGGAGCAGTGG
ATCGCTGAGAGGGAGGTGGTCGCAGGGTCCCATGAACTGGGACAGGACTATGAGCATGTC
ACGATGTTACAAGAACGATTCCGGGAGTTTGCCCGAGACACCGGGAACATTGGGCAGGAG
CGCGTGGACACGGTCAATCACCTGGCAGATGAGCTCATCAACTCTGGACATTCAGATGCC
GCCACCATCGCTGAATGGAAGGATGGCCTCAATGAAGCCTGGGCCGACCTCCTGGAGCTC
ATTGACACAAGAACACAGATTCTTGCCGCTTCCTATGAACTGCACAAGTTTTACCACGAT
GCCAAGGAGATCTTTGGGCGTATACAGGACAAACACAAGAAACTCCCTGAGGAGCTTGGG
AGAGATCAGAACACAGTGGAGACCTTACAGAGAATGCACACTACATTTGAGCATGACATC
CAGGCTCTGGGCACACAGGTGAGGCAGCTGCAGGAGGATGCAGCCCGCCTCCAGGCGGCC
TATGCGGGTGACAAGGCCGACGATATCCAGAAGCGCGAGAACGAGGTCCTGGAAGCCTGG
AAGTCCCTCCTGGACGCCTGTGAGAGCCGCAGGGTGCGGCTGGTGGACACAGGGGACAAG
TTCCGCTTCTTCAGCATGGTGCGCGACCTCATGCTCTGGATGGAGGATGTCATCCGGCAG
ATCGAGGCCCAGGAGAAGCCAAGGGATGTATCATCTGTTGAACTCTTAATGAATAATCAT
CAAGGCATCAAAGCTGAAATTGATGCACGTAATGACAGTTTCACAACCTGCATTGAACTT
GGGAAATCCCTGTTGGCGAGAAAACACTATGCATCTGAGGAGATCAAGGAAAAATTACTG
CAGTTGACGGAAAAGAGGAAAGAAATGATCGACAAGTGGGAAGACCGATGGGAATGGTTA
AGACTGATTCTGGAGGTCCATCAGTTCTCAAGAGACGCCAGTGTGGCCGAGGCCTGGCTG
CTTGGACAGGAGCCGTACCTATCCAGCCGAGAGATAGGCCAGAGCGTGGACGAGGTGGAG
AAGCTCATCAAGCGCCACGAGGCATTTGAAAAGTCTGCAGCAACCTGGGATGAGAGGTTC
TCTGCCCTGGAAAGGCTGACTACATTGGAGTTACTGGAAGTGCGCAGACAGCAAGAGGAA
GAGGAGAGGAAGAGGCGGCCGCCTTCTCCCGAGCCGAGCACGAAGGTTTCAGAGGAAGCC
GAGTCCCAGCAGCAGTGGGATACTTCAAAAGGAGAACAAGTTTCCCAAAACGGTTTGCCA
GCTGAACAGGGATCTCCACGGGTTAGTTACCGCTCTCAAACCTACCAAAACTACAAAAAC
TTTAATAGCAGACGGACAGCCAGTGACCAGCCATGGTCTGGACTG
Protein sequence predicted based on nucleotide sequence
MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSC RITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENM GSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNV NIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDP EDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASD LLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKM RANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAM RETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENY HDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLS QDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRV AHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSV MRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLS AIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEI ANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYK MFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIAR QLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIRE KTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAI LSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNT LTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNE LHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQED FMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKD NRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKI EKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLD KWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKST DEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDH GHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQL WGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKK HQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGK LDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQE RVDTVNHLADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHD AKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAA YAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQ IEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLL QLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVE KLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEA ESQQQWDTSKGEQVSQNGLPAEQGSPRVSYRSQTYQNYKNFNSRRTASDQPWSGL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
