Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746048
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3213nts
Open reading frame: 1 to 3213
ATGACGTCTCCAGCCAAATTCAAAAAGGATAAGGAGATCATAGCAGAGTACGATACTCAG
GTCAAAGAGATCCGTGCTCAGCTCACAGAGCAGATGAAATGCCTGGACCAGCAGTGTGAG
CTTCGGGTGCAACTGTTGCAGGACCTCCAGGACTTCTTCCGAAAGAAGGCAGAGATTGAG
ATGGACTACTCCCGCAACCTGGAGAAGCTGGCAGAACGCTTCCTGGCCAAGACACGCAGC
ACCAAGGACCAGCAATTCAAGAAGGATCAGAATGTTCTCTCTCCAGTCAACTGCTGGAAT
CTCCTCTTAAACCAGGTGAAGCGGGAAAGCAGGGACCATACCACCCTGAGTGACATCTAC
CTGAATAATATCATTCCTCGATTTGTACAAGTCAGCGAGGACTCAGGAAGACTCTTTAAA
AAGAGTAAAGAAGTCGGCCAGCAGCTCCAAGATGATTTGATGAAGGTCCTGAACGAGCTC
TACTCGGTGATGAAGACATATCACATGTACAATGCCGACAGCATCAGTGCTCAGAGCAAA
CTAAAGGAGGCGGAGAAGCAGGAGGAGAAGCAAATTGGTAAATCGGTAAAGCAGGAGGAC
CGGCAGACCCCACGCTCCCCTGACTCCACGGCCAACGTTCGCATTGAGGAGAAACATGTC
CGGAGGAGCTCAGTGAAGAAGATTGAGAAGATGAAGGAGAAGCGTCAAGCCAAGTACACG
GAGAATAAGCTGAAGGCCATCAAAGCCCGGAATGAGTACTTGCTGGCTTTGGAGGCAACC
AATGCATCTGTCTTCAAGTACTACATCCATGACCTATCTGACCTTATTGATCAGTGTTGT
GACTTAGGCTACCATGCAAGTCTGAACCGGGCTCTACGCACCTTCCTCTCTGCTGAGTTA
AACCTGGAACAGTCGAAGCATGAGGGTCTGGATGCCATCGAGAATGCAGTAGAAAACCTG
GATGCCACCAGTGACAAGCAGCGCCTCATGGAGATGTACAACAACGTCTTCTGCCCCCCT
ATGAAGTTTGAGTTTCAGCCCCACATGGGGGATATGGCTTCCCAGCTCTGTGCCCAGCAG
CCTGTCCAGAGTGAGCTGGTACAGAGATGCCAACAACTGCAGTCTCGCTTATCCACTCTA
AAGATTGAAAACGAAGAGGTAAAGAAGACAATGGAGGCCACCCTGCAAACCATCCAGGAC
ATTGTGACTGTCGAGGACTTTGATGTGTCTGACTGCTTCCAGTACAGCAACTCCATGGAG
TCCGTCAAGTCCACGGTCTCTGAAACCTTCATGAGCAAGCCCAGCATTGCTAAGAGGAGA
GCCAACCAGCAAGAGACAGAGCAGTTTTATTTCACAAAAATGAAAGAGTACCTGGAGGGC
AGGAACCTCATCACCAAGTTACAAGCCAAGCATGACCTTCTGCAGAAAACCCTGGGAGAA
AGTCAGCGGACAGATTGCAGTCTAGCCAGGCGCAGCTCAACTGTGAGGAAACAGGACTCC
AGCCAGGCAATTCCTCTAGTGGTGGAAAGCTGTATCCGGTTTATCAGCAGACACGGACTA
CAGCATGAAGGAATTTTCCGGGTGTCAGGATCCCAGGTGGAAGTGAATGACATCAAAAAT
GCCTTTGAGAGAGGAGAGGACCCCCTGGCTGGGGACCAGAACGACCATGACATGGATTCC
ATAGCTGGTGTCCTGAAGCTTTACTTCCGGGGGCTGGAACACCCTCTCTTCCCCAAGGAC
ATCTTTCATGACCTGATGGCCTGCGTCACAATGGACAACCTGCAGGAGAGAGCTCTGCAC
ATCCGGAAAGTCCTCCTAGTCCTGCCCAAAACCACTCTGATTATCATGAGATACCTCTTT
GCCTTCCTCAATCATTTATCACAGTTCAGTGAAGAGAACATGATGGACCCCTACAACCTC
GCCATCTGCTTCGGGCCCTCGCTAATGTCAGTGCCAGAGGGCCACGACCAGGTGTCCTGC
CAAGCCCACGTGAATGAGCTGATCAAAACCATCATCATCCAGCATGAGAACATCTTCCCA
AGCCCCAGGGAGCTGGAGGGCCCTGTCTACAGCAGAGGAGGAAGCATGGAGGATTACTGT
GATAGCCCTCATGGAGAGACTACCTCGGTTGAAGACTCAACCCAGGATGTGACCGCAGAG
CACCACACGAGCGATGACGAATGTGAGCCCATCGAGGCCATTGCCAAGTTTGACTACGTG
GGCCGGACAGCCCGAGAGCTGTCCTTTAAGAAGGGAGCATCCCTGCTGCTTTACCAGCGG
GCTTCCGACGACTGGTGGGAAGGCCGGCACAATGGCATCGACGGACTCATCCCCCATCAG
TACATCGTGGTCCAAGACACCGAGGACGGTGTCGTGGAGAGGTCCAGCCCCAAGTCTGAG
ATTGAGGTCATTTCTGAGCCACCTGAAGAAAAGGTGACAGCCAGAGCGGGGGCCAGCTGT
CCCAGTGGGGGTCATGTAGCCGATATTTATCTTGCAAACATCAACAAGCAAAGGAAGCGT
CCAGAATCTGGGAGCATCCGGAAAACTTTTCGGAGTGACAGCCATGGGCTGAGCAGTTCC
CTGACTGACTCCTCCTCCCCAGGGGTGGGGGCTAGCTGCCGCCCATCCTCCCAGCCCATC
ATGAGCCAGAGCCTCCCCAAAGAAGGGCCAGATAAGTGTTCCATCAGTGGGCACGGGAGC
CTCAACTCCATCAGCCGCCACTCATCCCTGAAGAATCGGCTGGATAGTCCACAGATCCGG
AAGACTGCCACAGCGGGAAGGTCAAAAAGCTTCAATAACCATCGGCCCATGGACCCTGAG
GTCATTGCTCAGGATATTGAGGCAACAATGAACTCGGCCCTGAATGAGCTACGGGAACTA
GAACGGCAGAGCAGTGTCAAACACACCCCTGACGTGGTTCTGGACACCTTGGAGCCCCTC
AAAACCTCCCCAGTGGTGGCCCCCACGTCAGAGCCCTCCAGCCCTCTGCACACCCAGCTC
CTCAAGGACCCCGAGCCCGCCTTCCAGCGCAGCGCCAGTACTGCTGGGGACATCGCCTGC
GCCTTCCGGCCTGTCAAGTCTGTCAAGATGGCTGCCCCGGTCAAACCACCAGCCACACGG
CCCAAGCCCACTGTCTTCCCCAAAACAAATGCCACTAGCCCTGGTGTCAACTCATCAACT
TCCCCACAGTCTACTGACAAGTCTTGTACTGTC
Protein sequence predicted based on nucleotide sequence
MTSPAKFKKDKEIIAEYDTQVKEIRAQLTEQMKCLDQQCELRVQLLQDLQDFFRKKAEIE MDYSRNLEKLAERFLAKTRSTKDQQFKKDQNVLSPVNCWNLLLNQVKRESRDHTTLSDIY LNNIIPRFVQVSEDSGRLFKKSKEVGQQLQDDLMKVLNELYSVMKTYHMYNADSISAQSK LKEAEKQEEKQIGKSVKQEDRQTPRSPDSTANVRIEEKHVRRSSVKKIEKMKEKRQAKYT ENKLKAIKARNEYLLALEATNASVFKYYIHDLSDLIDQCCDLGYHASLNRALRTFLSAEL NLEQSKHEGLDAIENAVENLDATSDKQRLMEMYNNVFCPPMKFEFQPHMGDMASQLCAQQ PVQSELVQRCQQLQSRLSTLKIENEEVKKTMEATLQTIQDIVTVEDFDVSDCFQYSNSME SVKSTVSETFMSKPSIAKRRANQQETEQFYFTKMKEYLEGRNLITKLQAKHDLLQKTLGE SQRTDCSLARRSSTVRKQDSSQAIPLVVESCIRFISRHGLQHEGIFRVSGSQVEVNDIKN AFERGEDPLAGDQNDHDMDSIAGVLKLYFRGLEHPLFPKDIFHDLMACVTMDNLQERALH IRKVLLVLPKTTLIIMRYLFAFLNHLSQFSEENMMDPYNLAICFGPSLMSVPEGHDQVSC QAHVNELIKTIIIQHENIFPSPRELEGPVYSRGGSMEDYCDSPHGETTSVEDSTQDVTAE HHTSDDECEPIEAIAKFDYVGRTARELSFKKGASLLLYQRASDDWWEGRHNGIDGLIPHQ YIVVQDTEDGVVERSSPKSEIEVISEPPEEKVTARAGASCPSGGHVADIYLANINKQRKR PESGSIRKTFRSDSHGLSSSLTDSSSPGVGASCRPSSQPIMSQSLPKEGPDKCSISGHGS LNSISRHSSLKNRLDSPQIRKTATAGRSKSFNNHRPMDPEVIAQDIEATMNSALNELREL ERQSSVKHTPDVVLDTLEPLKTSPVVAPTSEPSSPLHTQLLKDPEPAFQRSASTAGDIAC AFRPVKSVKMAAPVKPPATRPKPTVFPKTNATSPGVNSSTSPQSTDKSCTV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
