Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746058
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6963nts
Open reading frame: 1 to 6963
ATGGGGCCTGGAGCCCGTGGCCGACGCCGCCGACGTCGCCCGATGTCGCCGCCACCTCCA
CCACCTCCTGTTCGGGCGCTTCCCCTGCTGTTACTGCTGGCTGGGCCGGGGGCAGCAGCA
CCCCCTTGCTTGGACGGAAGCCCGTGTGCCAATGGTGGTCGTTGCACCCAACTTCCCTCC
CGCGAGGCGGCCTGCCTGTGCCCTCCAGGATGGGTGGGTGAGCGCTGCCAGCTGGAGGAC
CCTTGCCACAGCGGTCCCTGCGCTGGACGTGGTGTCTGCCAGAGTTCAGTCGTGGCTGGC
ACCGCCCGATTCTCCTGCCGGTGCCCCCGTGGATTCAGAGGCCCTGACTGCTCCCTGCCA
GATCCCTGTCTCAGCAGCCCTTGTGCCCACGGTGCCCGCTGCTCAGTGGGGCCAGATGGC
CGCTTTCTCTGCTCTTGCCCACCTGGCTATCAGGGCCGAAGCTGCCGAAGTGACGTCGAT
GAATGCCGAGTGGGCGAACCCTGCCGCCACGGTGGGACCTGCCTGAATACCCCTGGATCC
TTCCGATGCCAGTGTCCAGCGGGCTACACAGGGCCACTATGTGAGAATCCCGCGGTGCCC
TGTGCTCCTTCACCCTGTAGAAACGGGGGTACTTGCAGACAGAGTGGCGACCTCACTTAC
GACTGCGCCTGCCTTCCTGGCTTTGAGGGTCAGAATTGTGAAGTTAATGTGGACGATTGC
CCAGGCCACCGATGTCTCAATGGGGGAACATGTGTGGATGGCGTAAACACCTATAACTGC
CAATGTCCTCCTGAGTGGACAGGCCAATTCTGTACGGAGGACGTTGATGAATGTCAGCTG
CAACCCAATGCCTGTCACAATGGAGGTACATGCTTCAACACGCTGGGCGGCCACTCTTGC
GTGTGTGTCAATGGCTGGACAGGAGAAAGTTGCAGCCAAAATATCGACGACTGTGCGACT
GCTGTGTGCTTTCATGGCGCCACTTGCCATGACCGAGTGGCTTCTTTCTACTGTGCATGC
CCTATGGGAAAGACCGGACTCCTCTGTCACCTGGATGACGCATGTGTCAGCAATCCTTGT
CACGAGGATGCTATCTGTGACACAAATCCGGTGAACGGCCGAGCCATTTGCACCTGTCCT
CCCGGCTTCACCGGCGGTGCATGTGACCAGGATGTCGACGAATGCTCTATCGGCGCCAAT
CCCTGCGAACACTTGGGCAGATGCGTTAACACGCAGGGCTCTTTCCTATGTCAGTGCGGT
CGTGGATACACAGGCCCTCGGTGTGAGACAGATGTCAACGAGTGTTTGTCGGGGCCCTGC
CGAAACCAAGCCACGTGCCTCGACCGAATAGGCCAGTTCACATGTATCTGTATGGCAGGC
TTCACGGGGACCTATTGCGAGGTGGACATCGACGAGTGTCAGAGTAGCCCCTGTGTCAAC
GGTGGGGTGTGCAAAGACCGAGTCAACGGATTCAGTTGCACTTGCCCCTCGGGCTTCAGC
GGATCCACGTGTCAGCTCGACGTGGATGAATGTGCTAGTACGCCCTGCAGGAATGGAGCC
AAATGCGTGGACCAGCCCGATGGCTATGAGTGTAGATGTGCCGAGGGCTTTGAAGGCACC
CTGTGTGATCGCAACGTGGACGACTGCTCACCTGACCCATGTCACCACGGCCGCTGCGTG
GATGGAATCGCGAGCTTCTCATGTGCCTGCGCGCCCGGCTACACGGGCACACGCTGTGAG
TCACAGGTGGACGAGTGCCGCAGCCAACCCTGTCGCCACGGCGGCAAATGCCTGGACCTG
GTGGACAAGTACCTCTGCCGCTGCCCTTCTGGAACCACAGGTGTCAACTGCGAAGTGAAC
ATTGACGATTGTGCCAGCAACCCTTGTACATTTGGAGTCTGTCGTGATGGCATCAACCGC
TACGATTGCGTCTGCCAACCTGGATTTACGGGGCCCCTTTGTAACGTGGAGATCAATGAG
TGTGCTTCCAGTCCATGCGGCGAGGGAGGTTCATGTGTTGATGGGGAAAATGGATTCCGC
TGTCTCTGTCCGCCTGGGTCCTTGCCCCCACTCTGCCTGCCCCCCTCCCACCCCTGCGCC
CATGAGCCCTGTAGCCACGGAATCTGCTACGATGCACCTGGCGGGTTTCGCTGTGTGTGT
GAGCCAGGCTGGAGTGGCCCCCGCTGCAGCCAGAGCCTGGCCCGGGATGCCTGTGAATCC
CAGCCGTGCAGGGCCGGTGGTACCTGCAGCAGCGATGGAATGGGTTTCCATTGCACATGC
CCGCCGGGTGTTCAAGGACGTCAATGCGAACTCCTGTCACCCTGCACCCCAAACCCCTGT
GAGCATGGGGGCCGATGCGAGTCTGCCCCTGGCCAGCTCCCTGTTTGCTCATGCCCCCAA
GGCTGGCAAGGCCCAAGGTGCCAACAGGATGTTGATGAGTGCGCGGGCCCCGCACCTTGC
GGCCCTCATGGTATCTGCACAAACCTGGCAGGTAGTTTTAGCTGCACATGTCATGGAGGG
TACACTGGACCTTCCTGCGATCAGGACATCAATGACTGTGACCCTAATCCATGTCTTAAT
GGTGGATCGTGCCAAGACGGCGTGGGCTCCTTTTCCTGTTCTTGCCTCCCTGGTTTCGCA
GGACCTCGATGCGCCCGAGATGTCGATGAGTGCCTGTCTAACCCTTGCGGGCCGGGCACA
TGTACAGACCACGTGGCCTCATTCACCTGTACCTGCCCGCCTGGCTACGGAGGCTTTCAT
TGCGAACAAGACCTCCCCGATTGCAGCCCCAGCTCCTGTTTCAATGGCGGCACCTGTGTC
GATGGCGTGAACTCGTTCAGCTGCTTGTGTCGTCCCGGATATACAGGCGCCCACTGCCAG
CATGAAGCAGACCCCTGCCTCTCGCGGCCCTGTCTTCATGGGGGTGTATGCAGCGCCGCC
CACCCCGGATTTCGCTGCACTTGTTTGGAGAGCTTCACAGGACCGCAATGCCAGACGCTG
GTGGATTGGTGCTCCCGCCAACCTTGTCAAAATGGCGGTCGGTGCGTCCAGACTGGTGCC
TATTGCCTCTGTCCCCCCGGATGGAGCGGACGCCTCTGCGACATCCGAAGCTTGCCTTGC
CGAGAGGCCGCAGCTCAGATCGGCGTGCGCTTGGAACAGCTGTGTCAGGCTGGTGGCCAG
TGTGTTGACGAGGACAGCTCTCACTACTGCGTTTGCCCAGAAGGCCGTACCGGTAGCCAC
TGCGAGCAGGAAGTGGACCCATGCTTGGCCCAACCATGTCAACATGGCGGGACATGCCGT
GGATATATGGGAGGCTATATGTGTGAGTGTCTTCCTGGATACAATGGTGATAACTGTGAG
GACGACGTTGACGAATGTGCCTCCCAGCCCTGCCAACACGGGGGTTCATGCATTGACCTC
GTGGCTCGCTATCTTTGCTCCTGCCCACCAGGAACGCTGGGGGTCCTATGCGAGATTAAT
GAAGATGACTGCGGACCGGGCCCACCCTTGGACTCTGGGCCCCGGTGTCTACATAATGGA
ACCTGCGTCGACCTTGTGGGTGGTTTCCGTTGCACGTGTCCCCCAGGATACACCGGTCTT
CGCTGCGAGGCAGACATTAATGAGTGTCGCTCCGGCGCATGTCACGCGGCACACACCCGG
GACTGTCTCCAGGACCCCGGCGGAGGTTTTCGTTGCCTTTGTCATGCGGGCTTCTCAGGT
CCTCGCTGTCAGACTGTCCTGTCTCCTTGCGAGTCACAACCATGCCAACATGGAGGCCAG
TGCCGTCCTAGCCCCGGTCCTGGCGGTGGGCTGACCTTTACCTGTCATTGTGCTCAGCCG
TTTTGGGGTCCGCGTTGCGAGAGGGTGGCGCGGTCATGCCGGGAGCTGCAGTGCCCGGTG
GGCGTCCCATGCCAGCAGACGCCCAGAGGGCCGAGATGCGCTTGCCCTCCTGGGTTATCG
GGACCCTCCTGTCGCAGCTTCCCGGGATCGCCGCCCGGCGCTAGTAACGCCTCTTGCGCG
GCTGCGCCATGTCTCCACGGAGGATCCTGCCGTCCCGCGCCCCTCGCGCCCTTCTTCCGC
TGCGCTTGTGCACAGGGATGGACCGGACCTCGGTGCGAAGCGCCGGCCGCGGCACCCGAA
GTATCGGAGGAACCGCGGTGTCCGCGTGCCGCCTGTCAGGCCAAGCGCGGGGATCAGCGC
TGCGACCGCGAGTGCAACAGCCCTGGGTGTGGCTGGGACGGGGGCGACTGTTCGCTGAGC
GTGGGCGATCCTTGGAGGCAATGCGAGGCGCTACAGTGCTGGCGCCTCTTCAACAACAGC
CGGTGTGACCCCGCTTGCTCCTCCCCCGCATGCCTCTACGACAACTTCGACTGCCACGCC
GGTGGCCGAGAGCGCACTTGCAACCCGGTGTACGAGAAGTATTGCGCTGACCACTTTGCC
GATGGCCGGTGCGACCAGGGATGCAACACGGAAGAGTGCGGATGGGATGGGCTGGATTGT
GCTAGCGAGGTGCCGGCCTTGCTGGCCCGCGGCGTGCTGGTGCTTACAGTTCTGTTACCG
CCAGAGGAGCTACTCCGTTCCAGCGCCGACTTTCTGCAACGGCTCAGCGCCATCCTGCGC
ACCTCTCTGCGCTTTCGCCTGGACGCGCACGGCCAGGCCATGGTCTTCCCTTACCATCGC
CCTAGTCCCGGCTCCGAACCCCGAGCTCGTCGGGAGCTCGCTCCTGAGGTGATCGGCTCC
GTAGTAATGCTGGAGATTGACAACCGGCTCTGCCTCCAGTCACCCGAGAACGATCACTGC
TTCCCCGATGCCCAGAGCGCCGCTGACTACCTGGGAGCGTTGTCAGCGGTGGAGCGGTTG
GACTTCCCGTACCCACTGCGCGACGTGCGAGGGGAGCCGCTGGAGCCTCCAGAACCAAGT
GTCCCTCTGCTTCCACTGCTAGTCGCGGGCGCTGTGTTGTTGCTGGTCATTCTCGTCCTT
GGTGTCATGGTGGCCCGCCGCAAGCGCGAGCACAGCACCCTCTGGTTCCCTGAGGGGTTC
TCACTCCACAAGGATGTGGCCTCTGGTCATAAGGGCCGGCGGGAACCCGTGGGCCAGGAC
GCGTTGGGCATGAAGAACATGGCCAAGGGCGAGAGTCTGATGGGAGAGGTGGCCACAGAC
TGGATGGACACCGAGTGCCCTGAAGCCAAGCGGCTAAAGGTAGAGGAGCCAGGTATGGGG
GCTGAGGAGGCTGTCGATTGCCGGCAGTGGACTCAACACCATCTCGTTGCTGCTGACATC
CGCGTGGCACCAGCTATGGCACTTACACCACCACAGGGCGACGCAGATGCTGACGGCATG
GATGTCAATGTGCGCGGCCCAGATGGATTCACCCCGCTAATGCTCGCTTCCTTCTGTGGC
GGGGCTCTCGAGCCAATGCCAACTGAAGAGGATGAGGCAGATGACACTTCAGCTAGTATC
ATCTCCGACCTGATCTGCCAGGGCGCCCAGCTTGGGGCACGGACTGACCGTACTGGCGAG
ACTGCTTTACACTTGGCTGCCCGTTATGCCCGTGCTGATGCCGCTAAGCGGCTCTTGGAT
GCCGGGGCCGACACCAATGCCCAGGACCACTCCGGCCGCACTCCCCTGCATACCGCAGTG
ACAGCCGATGCACAAGGTGTGTTCCAGATTCTCATCCGAAACCGCTCAACAGATTTGGAT
GCTCGCATGGCCGATGGATCCACCGCATTGATTCTGGCGGCTCGACTGGCAGTAGAGGGC
ATGGTAGAAGAGCTCATCGCCAGCCATGCTGATGTGAATGCTGTGGATGAGCTTGGGAAA
TCAGCCTTACACTGGGCAGCTGCTGTGAACAATGTGGAAGCCACCTTGGCCCTGCTCAAA
AATGGTGCCAATAAGGACATGCAGGATAGCAAGGAGGAAACCCCCCTATTCCTGGCCGCT
CGGGAGGGGAGTTATGAGGCTGCTAAGCTTCTGTTGGACCATTTTGCCAACCGTGAGATC
ACCGACCACCTGGACCGACTCCCCCGGGACGTAGCCCAGGAGAGACTGCACCAGGATATC
GTGCGCCTCCTGGATCAACCTAGTGGGCCTCGAAGCCCTCCCGGTCCCCACGGCCTGGGG
CCTCTTCTCTGCCCTCCGGGGGCCTTTCTCCCTGGGCTCAAAGCGGCACAGTCCGGGTCT
AAGAAGAGCAGGAGGCCCCCAGGGAAGGCCGGGCTCGGGCCTCAGGGGCCTAGAGGGCGG
GGTAAGAAGCTGACGCTGGCCTGCCCGGGCCCCCTGGCTGACAGCTCCGTCACGCTGTCG
CCCGTGGACTCGCTGGACTCCCCGCGGCCTTTCGGAGGACCTCCTGCCTCCCCAGGGGGA
TTCCCCCTTGAGGGGCCCTATGCAGCCGCTACTGCTACTGCCGTGTCTCTCGCACAGCTT
GGCGGCCCGGGGCGGGCGGGTCTAGGGCGGCAGCCGCCTGGAGGATGTGTACTCAGCCTG
GGCCTGCTGAACCCTGTTGCTGTGCCTCTGGATTGGGCTCGCCTGCCACCACCCGCGCCT
CCAGGGCCCTCGTTCCTGCTACCACTGGCCCCTGGGCCACAGCTCCTCAACCCAGGGACC
CCCGTCTCCCCGCAGGAGCGGCCTCCGCCTTACCTCGCAGTCCCAGGACACGGCGAGGAG
TACCCTGCGGCTGGGGCCCACAGTAGCCCTCCAAAGGCCCGCTTCCTGAGGGTTCCCAGT
GAGCACCCCTACCTGACACCATCCCCCGAATCCCCTGAGCACTGGGCCAGCCCCTCACCC
CCCTCTCTCTCCGACTGGTCCGAATCCACACCTAGCCCAGCAACTGCCACTGGGGCCATG
GCTACCACCACTGGAGCACTCCCTGCACAGCCACTTCCCCTTTCTGTTCCTAGCTCCCTT
GCTCAGGCTCAGACCCAACTCGGGCCCCAACCGGAAGTTACCCCCAAGAGACAAGTGTTG
GCC
Protein sequence predicted based on nucleotide sequence
MGPGARGRRRRRRPMSPPPPPPPVRALPLLLLLAGPGAAAPPCLDGSPCANGGRCTQLPS REAACLCPPGWVGERCQLEDPCHSGPCAGRGVCQSSVVAGTARFSCRCPRGFRGPDCSLP DPCLSSPCAHGARCSVGPDGRFLCSCPPGYQGRSCRSDVDECRVGEPCRHGGTCLNTPGS FRCQCPAGYTGPLCENPAVPCAPSPCRNGGTCRQSGDLTYDCACLPGFEGQNCEVNVDDC PGHRCLNGGTCVDGVNTYNCQCPPEWTGQFCTEDVDECQLQPNACHNGGTCFNTLGGHSC VCVNGWTGESCSQNIDDCATAVCFHGATCHDRVASFYCACPMGKTGLLCHLDDACVSNPC HEDAICDTNPVNGRAICTCPPGFTGGACDQDVDECSIGANPCEHLGRCVNTQGSFLCQCG RGYTGPRCETDVNECLSGPCRNQATCLDRIGQFTCICMAGFTGTYCEVDIDECQSSPCVN GGVCKDRVNGFSCTCPSGFSGSTCQLDVDECASTPCRNGAKCVDQPDGYECRCAEGFEGT LCDRNVDDCSPDPCHHGRCVDGIASFSCACAPGYTGTRCESQVDECRSQPCRHGGKCLDL VDKYLCRCPSGTTGVNCEVNIDDCASNPCTFGVCRDGINRYDCVCQPGFTGPLCNVEINE CASSPCGEGGSCVDGENGFRCLCPPGSLPPLCLPPSHPCAHEPCSHGICYDAPGGFRCVC EPGWSGPRCSQSLARDACESQPCRAGGTCSSDGMGFHCTCPPGVQGRQCELLSPCTPNPC EHGGRCESAPGQLPVCSCPQGWQGPRCQQDVDECAGPAPCGPHGICTNLAGSFSCTCHGG YTGPSCDQDINDCDPNPCLNGGSCQDGVGSFSCSCLPGFAGPRCARDVDECLSNPCGPGT CTDHVASFTCTCPPGYGGFHCEQDLPDCSPSSCFNGGTCVDGVNSFSCLCRPGYTGAHCQ HEADPCLSRPCLHGGVCSAAHPGFRCTCLESFTGPQCQTLVDWCSRQPCQNGGRCVQTGA YCLCPPGWSGRLCDIRSLPCREAAAQIGVRLEQLCQAGGQCVDEDSSHYCVCPEGRTGSH CEQEVDPCLAQPCQHGGTCRGYMGGYMCECLPGYNGDNCEDDVDECASQPCQHGGSCIDL VARYLCSCPPGTLGVLCEINEDDCGPGPPLDSGPRCLHNGTCVDLVGGFRCTCPPGYTGL RCEADINECRSGACHAAHTRDCLQDPGGGFRCLCHAGFSGPRCQTVLSPCESQPCQHGGQ CRPSPGPGGGLTFTCHCAQPFWGPRCERVARSCRELQCPVGVPCQQTPRGPRCACPPGLS GPSCRSFPGSPPGASNASCAAAPCLHGGSCRPAPLAPFFRCACAQGWTGPRCEAPAAAPE VSEEPRCPRAACQAKRGDQRCDRECNSPGCGWDGGDCSLSVGDPWRQCEALQCWRLFNNS RCDPACSSPACLYDNFDCHAGGRERTCNPVYEKYCADHFADGRCDQGCNTEECGWDGLDC ASEVPALLARGVLVLTVLLPPEELLRSSADFLQRLSAILRTSLRFRLDAHGQAMVFPYHR PSPGSEPRARRELAPEVIGSVVMLEIDNRLCLQSPENDHCFPDAQSAADYLGALSAVERL DFPYPLRDVRGEPLEPPEPSVPLLPLLVAGAVLLLVILVLGVMVARRKREHSTLWFPEGF SLHKDVASGHKGRREPVGQDALGMKNMAKGESLMGEVATDWMDTECPEAKRLKVEEPGMG AEEAVDCRQWTQHHLVAADIRVAPAMALTPPQGDADADGMDVNVRGPDGFTPLMLASFCG GALEPMPTEEDEADDTSASIISDLICQGAQLGARTDRTGETALHLAARYARADAAKRLLD AGADTNAQDHSGRTPLHTAVTADAQGVFQILIRNRSTDLDARMADGSTALILAARLAVEG MVEELIASHADVNAVDELGKSALHWAAAVNNVEATLALLKNGANKDMQDSKEETPLFLAA REGSYEAAKLLLDHFANREITDHLDRLPRDVAQERLHQDIVRLLDQPSGPRSPPGPHGLG PLLCPPGAFLPGLKAAQSGSKKSRRPPGKAGLGPQGPRGRGKKLTLACPGPLADSSVTLS PVDSLDSPRPFGGPPASPGGFPLEGPYAAATATAVSLAQLGGPGRAGLGRQPPGGCVLSL GLLNPVAVPLDWARLPPPAPPGPSFLLPLAPGPQLLNPGTPVSPQERPPPYLAVPGHGEE YPAAGAHSSPPKARFLRVPSEHPYLTPSPESPEHWASPSPPSLSDWSESTPSPATATGAM ATTTGALPAQPLPLSVPSSLAQAQTQLGPQPEVTPKRQVLA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
