Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746220
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5400nts
Open reading frame: 1 to 5400
ATGTTTAACAAATCATTTGGAACACCCTTTGGGGGTGGCACAGGTGGCTTTGGCACAACT
TCAACATTTGGACAGAATACTGGCTTTGGCACTACTAGTGGAGGGGCATTTGGAACATCT
GCATTTGGTTCTAGCAACAATACTGGAGGCCTCTTTGGAAATTCACAGACTAAACCAGGA
GGATTGTTTGGAACCAGTTCATTTAGCCAGCCAGCTACCTCCACAAGCACTGGCTTTGGG
TTTGGTACGTCAACAGGAACAGCAAATACCTTGTTTGGAACTGCAAGCACAGGGACCAGT
CTCTTCTCATCCCAAAACAATGCCTTTGCACAAAATAAACCAACTGGCTTTGGCAATTTT
GGAACCAGTACTAGCAGTGGAGGACTCTTTGGAACCACAAATACCACCTCTAATCCTTTT
GGCAGCACATCTGGCTCCCTCTTTGGGCCAAGTAGTTTTACAGCTGCTCCTACTGGGACT
ACTATTAAATTTAACCCTCCAACTGGTACAGATACTATGGTCAAAGCTGGAGTTAGCACT
AACATAAGTACCAAGCACCAGTGTATTACTGCTATGAAAGAATATGAAAGCAAGTCACTA
GAGGAACTTCGTTTAGAGGATTATCAGGCTAACAGGAAGGGCCCACAGAACCAGGTGGGA
GCAGGTACCACAACTGGCTTGTTTGGGTCTTCTCCAGCCACTTCCAGCGCAACAGGACTC
TTCAGCTCCTCCACCACTAATTCAGGCTTTGCATATGGTCAGAACAAAACTGCCTTTGGA
ACTAGTACAACTGGATTTGGAACAAATCCAGGTGGTCTTTTTGGCCAACAGAATCAGCAG
ACTACCAGCCTCTTCAGCAAACCATTTGGCCAGGCTACAACCACCCAGAACACTGGCTTT
TCCTTTGGTAATACCAGCACCATAGGACAGCCAAGCACCAACACCATGGGATTATTTGGA
GTAACCCAAGCCTCACAGCCTGGAGGTCTTTTTGGGACAGCTACAAACACCAGCACTGGG
ACAGCATTTGGAACAGGAACAGGTCTGTTTGGGCAGACCAATACTGGATTTGGTGCTGTT
GGTTCGACCCTGTTTGGCAATAACAAGCTTACTACATTTGGAAGCAGCACAACCAGTGCA
CCTTCATTTGGTACAACCAGTGGCGGGCTCTTTGGTTTTGGCACAAATACCAGTGGGAAT
AGTATTTTTGGAAGTAAACCAGCACCTGGGACTCTTGGAACTGGGCTTGGTGCAGGATTT
GGAACAGCTCTTGGTGCTGGACAGGCATCTTTGTTTGGGAACAACCAACCTAAGATTGGA
GGGCCTCTTGGTACAGGAGCCTTTGGGGCCCCTGGATTTAATACTACGACAGCCACTTTG
GGCTTTGGAGCCCCCCAGGCCCCAGTAGCTTTGACAGATCCAAATGCTTCTGCTGCCCAG
CAGGCTGTTCTCCAGCAGCACATCAATAGTCTAACATACTCACCTTTTGGAGACTCTCCT
CTCTTCCGGAATCCGATGTCAGACCCTAAGAAGAAGGAAGAGAGATTGAAACCAACAAAT
CCAGCAGCCCAGAAGGCTCTTACTACACCTACTCATTATAAACTGACACCCCGCCCTGCC
ACTAGAGTCCGGCCAAAGGCTTTACAAACAACAGGCACAGCCAAGTCACATCTCTTTGAT
GGGCTGGATGACGATGAACCATCCCTAGCCAATGGAGCATTCATGCCCAAGAAGAGCATT
AAGAAGTTGGTTTTGAAGAACCTTAATAATAGCAATCTCTTTTCTCCTGTTAATCGTGAT
TCAGAAAATCTAGCTTCACCATCTGAATATCCAGAAAATGGAGAGAGATTTAGTTTCCTA
AGCAAACCTGTTGATGAGAATCACCAGCAGGATGGAGATGAAGATTCCCTTGTTTCACAT
TTTTATACTAACCCTATTGCCAAACCTATTCCTCAAACCCCAGAAAGTGCTGGAAATAAA
CACAGCAACAGCAACAGTGTGGATGATACCATTGTTGCATTAAACATGCGTGCTGCTTTG
CGAAATGGGCTGGAAGGAAGCAGTGAAGAAACGTCTTTTCATGATGAGTCACTTCAGGAT
GACCGAGAAGAAATAGAAAATAATTCTTACCATATGCACCCAGCAGGTATTATTCTCACT
AAGGTTGGTTACTATACTATTCCATCTATGGATGACCTTGCTAAAATTACCAATGAAAAA
GGAGAGTGCATTGTCTCTGATTTCACTATTGGTCGGAAAGGTTATGGTTCAATCTATTTT
GAAGGAGATGTGAATTTGACAAATCTAAATTTGGATGATATTGTGCATATCCGGAGGAAA
GAAGTAGTTGTCTACTTAGATGATAACCAAAAACCACCTGTGGGTGAAGGGCTAAATAGG
AAGGCTGAAGTTACATTGGATGGAGTTTGGCCAACAGATAAAACATCTCGTTGTTTAATA
AAGAGCCCAGATCGCCTTGCTGATATCAACTATGAAGGAAGATTGGAAGCAGTTTCAAGG
AAACAGGGAGCTCAATTCAAAGAATACCGGCCTGAAACTGGTTCTTGGGTGTTTAAAGTC
TCCCATTTTTCTAAGTATGGCCTTCAGGATTCTGATGAAGAGGAGGAGGAGCATCCGTCT
AAAACTAGTACAAAGAAGTTGAAGACTGCTCCTTTGCCTCCTGCAAGCCAGACTACGCCC
TTGCAGATGGCTCTTAATGGCAAACCTGCACCTCCACCTCAGAGCCAGAGCCCAGAGGTG
GAGCAGTTAGGGAGGGTTGTGGAACTGGACAGTGACATGGTAGATATCACCCAGGAGCCA
GTTTTGGATACCATGTTAGAAGAGAGCATGCCTGAGGATCAGGAACCTGTGTCTGCCTCA
ACACATATTGCATCTTCACTGGGAATTAATCCACATGTCTTACAGATCATGAAAGCATCA
TTGCTTACTGATGAAGAAGATGTAGATATGGCACTGGATCAACGCTTCAGTCGCCTGCCT
TCCAAAGCAGATACTTCTCAAGAAATCTGTTCTCCCAGACTCCCCATTTCAGCATCCCAC
TCGTCGAAAACTCGTTCACTAGTTGGTGGGTTACTACAATCAAAATTTACAAGTGGAGCT
TTTCTTTCACCAAGTGTCTCTGTGCAAGAATGTCGTACTCCCAGAGCAGCATCTTTAATG
AATATCCCATCCACATCCTCTTGGTCTGTCCCTCCACCCCTGACTTCTGTGTTCACAATG
CCCAGCCCAGCCCCTGAGGTTCCGTTGAAAACAGTGGGTACACGTAGGCAACTAGGCCTA
GTCCCTCGTGAAAAGTCTGTCACCTATGGCAAGGGAAAACTCTTGATGGACATGGCCCTA
TTCATGGGACGCTCATTTCGTGTTGGTTGGGGCCCCAACTGGACTCTTGCTAATAGTGGA
GAACAGCTGAATGGCTCTCATGAACTAGAAAATCATCAGATTGCCGATTCCATGGAGTTT
GGATTCCTGCCCAATCCAGTAGCTGTTAAACCCCTAACTGAGTCCCCATTCAAAGTTCAC
TTAGAAAAACTCAGTTTGAGACAAAGAAAGCCAGATGAAGACATGAAATTATATCAGACA
CCTCTGGAGCTCAAATTAAAACACAGCACTGTCCATGTGGATGAACTGTGTCCTCTCATT
GTCCCCAATCTGGGAGTTGCTGTCATTCATGACTATGCAGATTGGGTTAAAGAAGCATCA
GGAGACTTACCAGAAGCACAAATTGTGAAGCACTGGAGCCTGACATGGACACTATGTGAA
GCCCTATGGGGCCACCTGAAGGAGCTTGACAGCCAGCTAAATGAACCCCGTGAATACATT
CAAATTCTGGAGCGAAGAAGAGCTTTCTCCCGCTGGCTATCCTGTACTGCCACACCTCAG
ATTGAAGAGGAAGTCTCCTTAACCCAAAAAAACAGCCCTGTGGAGGCTGTATTCAGCTAC
CTCACAGGCAAAAGGATCAGTGAGGCCTGCTCTCTGGCCCAGCAGTCAGGGGATCATCGT
CTTGCTCTTCTTTTATCTCAGTTTGTGGGTAGCCAGTCAGTCCGGGAGCTGCTCACCATG
CAGTTGGTGGACTGGCATCAGCTCCAAGCAGACTCCTTCATCCAGGATGAGAGACTGCGC
ATCTTTGCTCTGTTGGCTGGAAAACCGGTGTGGCAGCTCTCAGAAAAGAAGCAAATAAAC
GTGTGCTCCCAGTTGGATTGGAAACGCTCCCTGGCTATCCATCTTTGGTATTTGCTTCCA
CCAACAGCCTCCATTTCTAGGGCGCTCAGCATGTATGAAGAAGCATTTCAGAATACCTCT
GACAGTGACAGATATGCCTGCTCCCCACTTCCTTCGTATCTGGAGGGTTCTGGCTGTGTG
ATAGCGGAGGAGCAAAACTCACAGACACCACTTCGAGATGTCTGCTTTCACCTTCTAAAA
CTCTACAGTGACAGACATTATGATCTCAACCAGCTGCTGGAGCCTCGAAGCATAACAGCA
GATCCTTTGGACTACCGCCTAAGCTGGCACTTGTGGGAAGTGCTGAGGGCTCTTAACTAC
ACCCATCTCTCAGCGCAGTGTGAAGGTGTGCTACAGGCCAGTTACGCTGGCCAGCTTGAA
AGTGAGGGGCTCTGGGAGTGGGCCATCTTTGTCCTCCTGCACATTGACAACTCAGGCATA
CGTGAGAAGGCTGTTCGAGAGCTGCTTACCCGGCACTGCCAGCTGTTGGAGACTCCTGAA
TCTTGGGCTAAAGAGACTTTCCTTACCCAGAAGCTCCGTGTACCTGCCAAATGGATCCAC
GAGGCCAAAGCTGTGCGAGCACACATGGAATCTGACAAGCACTTAGAGGCCCTTTGCTTA
TTTAAGGCTGAGCACTGGAACCGCTGCCACAAGCTCATCATCCGACACTTAGCTTCTGAT
GCCATCATTAATGAGAACTATGACTACCTGAAGGGGTTCTTGGAAGACCTGGCACCTCCA
GAGCGCAGCAGCCTAATTCAGGATTGGGAAACATCTGGGCTTGTTTACCTGGACTATATT
AGAGTCATTGAAATGCTCCGCCATATACAGCAGGTTGATTGCTCAGGTAATGACCTGGAG
CAGTTACACATCAAAGTGACTTCACTGTGCAGTCGGATAGAGCAGATTCAGTGTTACAGT
GCTAAAGATCGCCTGGCTCAGTCAGACATGGCCAAACGTGTAGCCAACCTGCTGCGCGTG
GTGCTGAGTCTGCATCATCCTCCTGATAGAACCTCCGACTCAACACCAGACCCTCAGCGA
GTCCCTTTGCGCCTCTTGGCTCCCCACATTGGCCGGCTTCCCATGCCTGAGGACTATGCC
ATGGACGAACTGCGCAGCCTTACCCAGTCCTATCTGCGAGAACTGGCTGTTGGGAGCCTG
Protein sequence predicted based on nucleotide sequence
MFNKSFGTPFGGGTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGSSNNTGGLFGNSQTKPG GLFGTSSFSQPATSTSTGFGFGTSTGTANTLFGTASTGTSLFSSQNNAFAQNKPTGFGNF GTSTSSGGLFGTTNTTSNPFGSTSGSLFGPSSFTAAPTGTTIKFNPPTGTDTMVKAGVST NISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNQVGAGTTTGLFGSSPATSSATGL FSSSTTNSGFAYGQNKTAFGTSTTGFGTNPGGLFGQQNQQTTSLFSKPFGQATTTQNTGF SFGNTSTIGQPSTNTMGLFGVTQASQPGGLFGTATNTSTGTAFGTGTGLFGQTNTGFGAV GSTLFGNNKLTTFGSSTTSAPSFGTTSGGLFGFGTNTSGNSIFGSKPAPGTLGTGLGAGF GTALGAGQASLFGNNQPKIGGPLGTGAFGAPGFNTTTATLGFGAPQAPVALTDPNASAAQ QAVLQQHINSLTYSPFGDSPLFRNPMSDPKKKEERLKPTNPAAQKALTTPTHYKLTPRPA TRVRPKALQTTGTAKSHLFDGLDDDEPSLANGAFMPKKSIKKLVLKNLNNSNLFSPVNRD SENLASPSEYPENGERFSFLSKPVDENHQQDGDEDSLVSHFYTNPIAKPIPQTPESAGNK HSNSNSVDDTIVALNMRAALRNGLEGSSEETSFHDESLQDDREEIENNSYHMHPAGIILT KVGYYTIPSMDDLAKITNEKGECIVSDFTIGRKGYGSIYFEGDVNLTNLNLDDIVHIRRK EVVVYLDDNQKPPVGEGLNRKAEVTLDGVWPTDKTSRCLIKSPDRLADINYEGRLEAVSR KQGAQFKEYRPETGSWVFKVSHFSKYGLQDSDEEEEEHPSKTSTKKLKTAPLPPASQTTP LQMALNGKPAPPPQSQSPEVEQLGRVVELDSDMVDITQEPVLDTMLEESMPEDQEPVSAS THIASSLGINPHVLQIMKASLLTDEEDVDMALDQRFSRLPSKADTSQEICSPRLPISASH SSKTRSLVGGLLQSKFTSGAFLSPSVSVQECRTPRAASLMNIPSTSSWSVPPPLTSVFTM PSPAPEVPLKTVGTRRQLGLVPREKSVTYGKGKLLMDMALFMGRSFRVGWGPNWTLANSG EQLNGSHELENHQIADSMEFGFLPNPVAVKPLTESPFKVHLEKLSLRQRKPDEDMKLYQT PLELKLKHSTVHVDELCPLIVPNLGVAVIHDYADWVKEASGDLPEAQIVKHWSLTWTLCE ALWGHLKELDSQLNEPREYIQILERRRAFSRWLSCTATPQIEEEVSLTQKNSPVEAVFSY LTGKRISEACSLAQQSGDHRLALLLSQFVGSQSVRELLTMQLVDWHQLQADSFIQDERLR IFALLAGKPVWQLSEKKQINVCSQLDWKRSLAIHLWYLLPPTASISRALSMYEEAFQNTS DSDRYACSPLPSYLEGSGCVIAEEQNSQTPLRDVCFHLLKLYSDRHYDLNQLLEPRSITA DPLDYRLSWHLWEVLRALNYTHLSAQCEGVLQASYAGQLESEGLWEWAIFVLLHIDNSGI REKAVRELLTRHCQLLETPESWAKETFLTQKLRVPAKWIHEAKAVRAHMESDKHLEALCL FKAEHWNRCHKLIIRHLASDAIINENYDYLKGFLEDLAPPERSSLIQDWETSGLVYLDYI RVIEMLRHIQQVDCSGNDLEQLHIKVTSLCSRIEQIQCYSAKDRLAQSDMAKRVANLLRV VLSLHHPPDRTSDSTPDPQRVPLRLLAPHIGRLPMPEDYAMDELRSLTQSYLRELAVGSL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
