Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746353
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5373nts
Open reading frame: 1 to 5373
ATGGCCGGGGCTTCGGTGAAGGTGGCGGTGCGGGTCCGCCCCTTCAATTCCCGGGAAATG
AGCCGTGACTCCAAGTGCATCATTCAGATGTCTGGAAGCACCACCACCATTGTTAACCCC
AAACAGCCCAAGGAGACGCCCAAAAGCTTCAGCTTTGACTACTCCTACTGGTCGCACACC
TCACCAGAGGACATCAACTACGCGTCGCAGAAACAGGTGTACCGGGACATCGGCGAGGAG
ATGCTACAGCATGCCTTTGAGGGGTACAACGTGTGCATCTTCGCCTATGGGCAGACGGGT
GCCGGCAAGTCCTACACCATGATGGGCAAGCAGGAGAAGGACCAGCAGGGCATCATCCCA
CAGCTCTGCGAGGACCTCTTCTCTCGGATCAACGACACGACCAACGACAACATGTCGTAC
TCCGTGGAGGTCAGCTACATGGAGATTTACTGTGAGCGCGTCCGTGACCTACTGAACCCC
AAGAACAAGGGCAACCTGCGCGTGAGGGAGCACCCACTGCTGGGGCCCTACGTGGAGGAC
CTCTCGAAGCTGGCTGTGACCTCCTACAATGACATCCAGGACCTCATGGACTCAGGGAAC
AAGGCCAGAACCGTGGCGGCCACCAACATGAATGAGACAAGCAGTCGATCCCACGCCGTC
TTCAACATCATCTTCACGCAGAAGCGCCATGACGCAGAAACCAATATCACCACGGAGAAG
GTCAGCAAAATCAGCCTGGTGGACCTGGCTGGGAGCGAGCGGGCTGACTCCACGGGAGCC
AAGGGCACGCGCCTCAAGGAGGGGGCCAACATCAACAAGTCGCTGACCACACTGGGCAAG
GTCATCTCCGCCCTGGCTGAAATGGACTCCGGACCCAACAAGAACAAGAAAAAGAAGAAG
ACAGATTTCATTCCGTACCGAGATTCCGTGTTGACCTGGCTCCTCCGGGAAAACCTAGGC
GGTAACTCAAGGACAGCTATGGTGGCAGCCTTGAGTCCTGCAGACATCAACTACGATGAG
ACTCTTAGCACGCTGAGGTATGCTGACCGGGCCAAGCAGATCCGCTGCAATGCTGTCATC
AATGAGGACCCCAACAACAAGCTGATCCGCGAGCTGAAGGATGAGGTGACCCGGCTGCGG
GACCTTCTGTACGCCCAGGGTCTAGGCGACATCACTGACACCAACACTGTGCCTGGAGGA
CCCAAATTGACCAATGCCCTGGTGGGTATGAGCCCCTCATCCTCACTCTCAGCCCTGTCC
AGCCGCGCCGCCTCCGTGTCAAGCCTTCACGAGCGCATCTTGTTTGCCCCGGGCAGCGAG
GAGGCCATTGAAAGACTGAAGGAAACAGAGAAGATCATAGCTGAGCTCAATGAAACCTGG
GAGGAGAAGCTGCGGCGGACAGAAGCCATCCGCATGGAGAGGGAAGCCCTGCTGGCCGAG
ATGGGTGTGGCCATGAGGGAGGATGGCGGCACCTTGGGCGTATTCTCTCCCAAAAAGACA
CCACACCTCGTCAACTTGAACGAGGACCCACTGATGTCTGAGTGCTTGCTCTACTACATC
AAGGATGGGATCACCAGAGTGGGCAGGGAGGATGGGGAGAGGCGGCAGGACATTGTTCTG
AGTGGGCACTTCATCAAGGAGGAGCACTGCGTCTTCCGGAGCGACTCCAGGGGAGGCAGC
GAAGCTGTGGTGACCTTGGAGCCCTGTGAGGGGGCAGACACCTACGTCAATGGCAAAAAA
GTCACAGAGCCCAGCATCCTGCGTTCAGGAAACCGCATCATCATGGGTAAGAGCCATGTG
TTCCGGTTCAACCACCCCGAGCAGGCCCGGCAGGAGCGTGAGCGCACGCCTTGTGCGGAG
ACGCCAGCTGAGCCTGTGGACTGGGCCTTCGCCCAGCGTGAGCTTCTGGAGAAGCAGGGC
ATCGACATGAAGCAGGAGATGGAGCAGAGGCTCCAGGAACTGGAGGACCAGTACCGCCGC
GAGCGGGAGGAGGCCACCTACCTTCTGGAGCAGCAGCGGCTTGACTATGAGAGCAAGCTG
GAGGCTCTGCAGAAGCAGATGGACTCCAGGTACTACCCGGAGGTGAACGAGGAGGAGGAG
GAGCCCGAGGATGAAGTTCAGTGGACAGAGCGGGAGTGTGAGCTGGCGCTCTGGGCCTTC
CGGAAGTGGAAGTGGTACCAGTTCACGTCTCTGCGGGACCTGCTGTGGGGCAACGCCATC
TTCCTCAAGGAGGCAAATGCCATCAGCGTGGAGCTGAAAAAGAAGGTACAATTCCAGTTT
GTCCTCCTGACGGACACACTCTACTCCCCTCTGCCACCCGACCTACTGCCGCCAGAGGCT
GCCAAAGACCGAGAGACGCGGCCCTTCCCCCGCACCATTGTGGCCGTGGAGGTCCAGGAC
CAGAAGAACGGGGCCACCCACTACTGGACGCTGGAGAAGCTCAGGCAGCGTCTGGACCTG
ATGCGGGAGATGTACGACCGCGCTGCAGAGGTCCCCTCCAGTGTAATCGAGGACTGTGAC
AACGTGGTGACCGGCGGAGATCCCTTCTATGACCGCTTCCCCTGGTTCCGGCTGGTGGGC
AGTTCAGCCATCTCTGGCTGCAACAGCTACCCTCTTCTCAACACATGCATGAGCGAGCGC
ATGGCTGCTCTCACCCCCTCCCCCACCTTCTCGAGCCCCGACTCCGACGCCACGGAGCCT
GCCGAGGAGCAGAGCGTGGGGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGATGAGGAG
GAGGAGGACCTGGAGGACGACGTCTTTCCGGAGCACGCGCTGTGCGACGGCCGGGACCCG
TTTTACGACCGGCCCCCCCTGTTCAGTTTAGTAGGAAGGGCCTTCGTTTACCTGAGCAAC
CTGCTGTACCCCGTTCCCCTGGTACACCGTGTGGCTATCGTCAGCGAGAAGGGCGAGGTG
AAGGGCTTCCTCCGCGTGGCCGTCCAGGCCATCTCAGCCGATGAAGAGGCCCCTGATTAT
GGCTCTGGAGTCCGGCAGTCGGGAACTGCTAAAATCTCCTTTGATGACCAGCATTTTGAA
AAGTTCCAGTCCGAGTCTTGCCCCGTGGTGGGGATGTCCCGCTCGGGAACCTCCCAGGAA
GAGCTTCGCATCGTGGAGGGCCAGGGCCAGGGTGCAGACGTGGGACCCTCAGCCGATGAA
GTCAACAACAACACCTGTTCAGCAGTGCCCCCAGAAGGCCTCCTCCTAGACAGCTCTGAG
AAAGCCGCCCTGGATGGGCCCCTGGATGCTGCCCTGGACCACCTCCGCCTGGGCAACACC
TTCACCTTCCGTGTGACAGTCCTGCAGGCGTCCAGCATCTCTGCCGAATATGCCGACATC
TTCTGCCAGTTCAACTTCATCCACCGCCACGACGAGGCCTTCTCCACAGAGCCCCTGAAG
AACACAGGCAGAGGCCCCCCACTTGGCTTCTACCACGTCCAGAACATCGCAGTGGAGGTG
ACCAAGTCCTTCATTGAGTACATCAAGAGCCAGCCCATTGTTTTCGAGGTCTTTGGCCAC
TACCAGCAGCACCCGTTCCCGCCCCTCTGCAAGGACGTGCTCAGCCCACTGAGGCCCTCG
CGCCGCCACTTCCCTCGCGTCATGCCTCTGTCCAAGCCAGTGCCCGCCACCAAGCTCAGC
ACACTAACGCGGCCCTGTCCGGGACCCTGCCACTGCAAGTACGACCTGCTGGTCTACTTC
GAGATCTGTGAGCTGGAGGCCAACGGCGATTACATCCCGGCCGTGGTGGACCACCGTGGG
GGCATGCCATGCATGGGGACCTTCCTCCTCCACCAGGGCATCCAGCGACGGATTACGGTG
ACACTACTGCATGAGACAGGCAGCCATATCCGCTGGAAGGAAGTGCGCGAGCTGGTCGTG
GGCCGCATCCGAAACACTCCAGAAACCGACGAGTCCCTAATCGACCCCAACATCTTGTCT
CTCAACATCCTCTCTTCCGGATACATCCACCCAGCCCAAGATGACCGGACCTTTTACCAA
TTTGAGGCTGCGTGGGACAGCTCCATGCACAACTCTCTGCTGCTGAACCGGGTCACCCCT
TATCGAGAGAAAATATACATGACACTCTCCGCTTATATCGAGATGGAGAACTGCACCCAG
CCGGCTGTTGTCACCAAGGACTTCTGCATGGTCTTCTATTCCCGTGATGCCAAGCTGCCA
GCGTCGCGCTCCATCCGCAACCTCTTTGGCAGTGGGAGCCTTCGGGCCTCAGAGAGTAAC
CGTGTGACTGGTGTGTACGAGCTCAGCCTGTGCCACGTGGCTGACGCGGGCAGCCCAGGG
ATGCAGCGCCGGCGACGACGAGTCCTGGACACATCTGTGGCCTATGTCCGGGGCGAGGAG
AACCTGGCAGGCTGGAGGCCCCGAAGTGACAGTCTGATTCTGGACCACCAGTGGGAGCTG
GAGAAGCTGAGCCTCCTGCAGGAGGTGGAGAAGACTAGGCACTACCTGCTCCTGCGGGAG
AAGCTGGAGACTGCCCAGCGGCCTGTCCCGGAGGCACTGTCCCCGGCCTTCAGCGAGGAC
TCTGAGTCCCATGGCTCCTCCAGCGCCTCCTCCCCGCTCTCGGCTGAGGGCCGCCCATCA
CCCCTGGAGGCTCCCAACGAGAGGCAGCGTGAGCTGGCCGTCAAGTGCTTACGCCTGCTG
ACGCACACATTCAACAGAGAGTACACTCACAGTCACGTCTGCGTCAGTGCCAGCGAGAGC
AAGCTCTCCGAGATGTCTGTCACCCTGCTCCGGGACCCGTCGATGTCCCCCCTAGGGGTG
GCAACTCTCACCCCCTCCTCCACTTGCCCCTCTCTGGTTGAAGGGCGGTACGGTGCCACT
GACCTGAGGACCCCGCAGCCCTGCTCCCGGCCAGCCAGCCCAGAGCCCGAGCTGCTGCCA
GAGGCCGACTCCAAGAAGCTCCCTTCCCCTGCCCGGGCAACAGAGACAGACAAGGAGCCC
CAGCGCCTGCTGGTGCCTGACATCCAGGAGATCCGAGTCAGCCCGATCGTTTCCAAGAAG
GGGTACCTGCACTTCCTGGAGCCGCACACGTCAGGCTGGGCCAGGCGCTTCGTGGTGGTG
CGGCGCCCCTATGCTTACATGTACAACAGCGACAAGGACACCGTGGAGCGTTTCGTGCTC
AACCTGGCCACTGCCCAGGTGGAGTACAGTGAGGACCAGCAGGCTATGCTCAAGACACCT
AACACATTCGCGGTGTGCACGGAACACCGCGGGATCTTGCTGCAGGCCGCCAGCGACAAG
GACATGCATGACTGGCTGTACGCCTTCAACCCCCTCCTGGCCGGGACCATACGGTCCAAG
CTCTCTAGGAGGAGGTCTGCCCAGATGCGGGTC
Protein sequence predicted based on nucleotide sequence
MAGASVKVAVRVRPFNSREMSRDSKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHT SPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIP QLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVED LSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEK VSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKK TDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVI NEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTNTVPGGPKLTNALVGMSPSSSLSALS SRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAE MGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDGERRQDIVL SGHFIKEEHCVFRSDSRGGSEAVVTLEPCEGADTYVNGKKVTEPSILRSGNRIIMGKSHV FRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRR EREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAF RKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEA AKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSVIEDCD NVVTGGDPFYDRFPWFRLVGSSAISGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEP AEEQSVGEEEEEEEEEEDEEEEDLEDDVFPEHALCDGRDPFYDRPPLFSLVGRAFVYLSN LLYPVPLVHRVAIVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFE KFQSESCPVVGMSRSGTSQEELRIVEGQGQGADVGPSADEVNNNTCSAVPPEGLLLDSSE KAALDGPLDAALDHLRLGNTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLK NTGRGPPLGFYHVQNIAVEVTKSFIEYIKSQPIVFEVFGHYQQHPFPPLCKDVLSPLRPS RRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRG GMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILS LNILSSGYIHPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQ PAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPG MQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLRE KLETAQRPVPEALSPAFSEDSESHGSSSASSPLSAEGRPSPLEAPNERQRELAVKCLRLL THTFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGVATLTPSSTCPSLVEGRYGAT DLRTPQPCSRPASPEPELLPEADSKKLPSPARATETDKEPQRLLVPDIQEIRVSPIVSKK GYLHFLEPHTSGWARRFVVVRRPYAYMYNSDKDTVERFVLNLATAQVEYSEDQQAMLKTP NTFAVCTEHRGILLQAASDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
