Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746355
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5838nts
Open reading frame: 1 to 5838
ATGGTTGAATCTTGCCTACTCACGTTTAGAGCCTTTTTCTGGTGGATTGCTTTAATAAAG
ATGGATCTGTCAGACCTTGGAGAAGCCGCAGCCTTCCTCAGAAGAAGTGAAGCTGAGCTG
CTTCTACTACAGGCCACAGCCTTGGATGGGAAGAAGAAATGCTGGATTCCTGATGGTGAG
AATGCTTATATCGAGGCTGAGGTAAAAGGGAGTGAAGATGATGGAACAGTAATTGTTGAG
ACAGCAGATGGAGAGAGTCTGAGCATAAAGGAGGACAAAATCCAGCAGATGAATCCTCCA
GAGTTTGAAATGATTGAAGACATGGCAATGCTGACTCACCTCAATGAGGCATCCGTGCTG
CATACCCTGAAGCGGCGCTATGGCCAGTGGATGATCTATACCTATTCAGGTCTGTTCTGT
GTGACCATAAACCCTTACAAATGGCTTCCCGTGTATCAGAAAGAAGTCATGGCCGCCTAC
AAAGGGAAGAGGCGATCAGAGGCTCCCCCTCACATCTTTGCTGTTGCCAATAACGCCTTT
CAGGACATGCTCCACAATAGAGAGAATCAGTCTATACTCTTCACAGGAGAATCTGGTGCT
GGAAAGACTGTGAACAGCAAACATATTATCCAGTATTTTGCCACCATAGCAGCCATGATT
GAATCCAGGAAAAAGCAAGGCGCGTTAGAAGATCAAATCATGCAAGCGAATACTATCTTG
GAAGCATTTGGAAATGCTAAAACCCTGAGAAATGACAACTCCTCTCGTTTTGGCAAATTC
ATCAGGATGCACTTTGGTGCCAGAGGCATGCTGTCATCTGTGGACATTGATATCTATTTG
CTTGAAAAGTCCAGGGTGATTTTCCAGCAGGCTGGAGAGCGGAACTACCACATATTCTAT
CAAATTCTATCTGGACAAAAAGAGCTTCATGACCTGCTCCTGGTATCTGCAAATCCCTCC
GACTTCCACTTTTGCTCTTGTGGAGCAGTTACTGTGGAGAGCTTGGATGATGCTGAAGAA
TTGCTGGCCACAGAACAAGCCATGGACATCTTGGGCTTTCTTCCTGATGAGAAGTATGGA
TGCTATAAACTCACTGGAGCTATCATGCACTTTGGAAATATGAAATTTAAACAGAAACCT
AGAGAAGAGCAACTGGAAGCAGATGGCACAGAAAATGCTGACAAAGCCGCTTTCCTCATG
GGCATTAACTCCTCTGAGTTGGTAAAGTGCTTGATCCATCCTAGAATCAAAGTTGGTAAC
GAATATGTTACCAGAGGTCAAACTATAGAACAGGTAACCTGTGCTGTCGGTGCCCTGTCC
AAGTCAATGTATGAAAGGATGTTTAAGTGGCTAGTGGCACGGATCAACAGGGCCCTGGAT
GCCAAGCTGTCAAGGCAGTTCTTCATTGGCATTCTTGACATCACTGGTTTTGAAATCCTT
GAGTATAATAGCCTTGAGCAACTTTGCATTAATTTTACCAATGAAAAATTACAACAATTC
TTCAATTGGCACATGTTCGTTCTGGAGCAAGAGGAATATAAGAAAGAAAGCATTGAATGG
GTGTCTATTGGCTTTGGTCTGGATTTGCAAGCTTGCATAGATCTCATTGAGAAGCCAATG
GGCATCCTTTCTATCCTTGAAGAAGAGTGTATGTTTCCTAAGGCTACAGACCTGACTTTC
AAGACCAAACTCTTTGACAACCATTTTGGAAAGTCGGTTCACCTCCAGAAGCCCAAGCCT
GATAAGAAGAAATTTGAAGCTCATTTTGAACTTGTCCATTATGCAGGAGTGGTACCTTAT
AATATTAGCGGTTGGCTGGAAAAGAACAAAGACCTCCTTAATGAAACAGTGGTAGCTGTA
TTTCAGAAGTCTTCCAACAGACTCCTGGCCAGCCTTTTTGAAAATTACATGAGTACTGAC
AGTGCTATACCATTTGGGGAGAAGAAACGAAAGAAAGGAGCTTCATTCCAAACGGTTGCA
TCTCTGCATAAAGAAAACCTGAATAAATTGATGACTAATCTGAAATCAACAGCACCTCAT
TTTGTGAGATGCATAAATCCCAATGTCAACAAAATACCAGGTATACTGGACCCTTACTTG
GTTCTACAGCAGTTGCGCTGTAATGGTGTCTTGGAAGGGACTAGGATATGCCGTGAAGGC
TTTCCAAACCGACTGCAGTATGCTGATTTTAAACAAAGGTACTGCATACTGAATCCACGA
ACCTTTCCAAAGAGCAAGTTTGTGAGCAGCAGAAAGGCAGCTGAAGAATTACTTGGCTCC
TTGGAGATAGACCATACCCAGTACCGATTTGGAATCACTAAGGTGTTTTTTAAAGCTGGG
TTTCTGGGCCAACTGGAAGCGATAAGAGATGAGAGGCTATCTAAAGTCTTCACCTTGTTC
CAAGCCAGAGCACAGGGCAAACTGATGCGGATCAAATTCCAGAAGATTCTGGAAGAAAGG
GATGCACTTATCTTGATCCAATGGAACATCAGAGCTTTCATGGCTGTGAAGAACTGGCCC
TGGATGAGGCTCTTCTTCAAGATCAAGCCTCTTGTGAAATCTTCAGAAGTAGGAGAAGAA
GTAGCCGGACTGAAGGAAGAGTGTGCACAATTACAGAAAGCCTTGGAGAAATCAGAGTTT
CAGAGGGAGGAACTCAAAGCCAAGCAAGTATCCTTAACTCAAGAAAAAAATGACCTCATT
CTGCAGCTTCAGGCTGAGCAAGAGACACTGGCAAATGTTGAAGAGCAGTGCGAGTGGCTG
ATTAAATCCAAGATCCAGCTGGAGGCCAGAGTAAAGGAGCTGTCGGAGAGGGTGGAGGAA
GAAGAGGAGATAAATTCTGAGCTGACTGCCAGGGGGCGGAAACTGGAAGATGAATGTTTT
GAGCTGAAGAAAGAAATCGATGACCTGGAAACAATGTTGGTGAAGTCAGAGAAGGAGAAG
CGTACTACAGAGCACAAGGTCAAGAACTTGACTGAGGAAGTAGAGTTTCTAAATGAGGAT
ATCAGCAAACTTAACAGAGCAGCCAAGGTTGTGCAGGAGGCCCATCAGCAGACCCTGGAT
GACCTGCACATGGAGGAGGAGAAGCTCAGCAGCCTGAGCAAAGCAAATCTGAAGCTGGAA
CAGCAAGTTGATGAGCTTGAGGGGGCCCTTGAGCAGGAGAGAAAAGCGCGGATGAACTGT
GAAAGGGAACTGCACAAACTTGAGGGCAATTTAAAACTGAATCGGGAAAGCATGGAGAAC
CTTGAAAGCAGCCAGCGACACCTGGCAGAAGAGCTGAGGAAAAAAGAATTAGAATTGAGT
CAGATGAATTCAAAAGTGGAGAATGAGAAAGGCCTGGTAGCTCAGCTTCAGAAGACGGTT
AAAGAGCTTCAGACTCAAATAAAGGATTTGAAAGAGAAACTAGAAGCTGAAAGGACCACT
AGAGCCAAGATGGAAAGAGAGAGAGCTGACCTCACCCAAGACCTGGCTGACTTGAATGAG
AGGCTGGAGGAGGTAGGAGGATCCAGTTTGGCTCAGCTGGAAATAACTAAGAAACAGGAA
ACCAAATTCCAGAAGCTGCACCGAGACATGGAAGAGGCCACTCTTCACTTTGAGACAACT
TCTGCATCTTTGAAGAAGAGACATGCAGACAGCCTGGCTGAGCTCGAGGGCCAGGTAGAA
AATCTACAGCAGGTCAAACAGAAACTGGAAAAAGACAAGAGTGACTTGCAGCTAGAAGTA
GATGACCTCCTCACCCGTGTTGAGCAGATGACAAGAGCTAAGGCAAATGCTGAGAAACTC
TGTACCCTATATGAAGAGCGCTTGCATGAAGCAACTGCAAAGCTAGATAAGGTGACTCAG
TTGGCAAATGACCTTGCAGCACAAAAGACAAAGCTGTGGAGTGAGAGTGGCGAGTTCCTA
CGGAGGCTTGAAGAGAAGGAGGCTCTGATAAACCAACTTTCCAGGGAAAAGAGTAACTTC
ACTCGGCAGATTGAAGACCTGAGAGGGCAGCTGGAAAAGGAGACAAAATCCCAGAGTGCC
CTGGCCCATGCCCTGCAGAAGGCTCAGCGTGACTGTGACCTTCTACGAGAGCAGTATGAG
GAAGAACAAGAGGTCAAGGCTGAGCTGCACCGGACCTTATCCAAAGTCAATGCTGAAATG
GTGCAATGGAGAATGAAGTATGAAAACAATGTCATCCAGAGAACAGAAGACTTGGAGGAT
GCTAAGAAGGAACTGGCAATTAGATTGCAGGAGGCAGCCGAAGCCATGGGGGTGGCCAAT
GCCAGAAATGCCTCCTTGGAGAGAGCCAGGCACCAGCTGCAGCTGGAGCTTGGGGACGCC
CTGTCTGACCTCGGGAAGGTCCGCTCCGCAGCAGCCAGGCTGGACCAGAAGCAGCTGCAG
TCTGGCAAGGCCCTTGCCGACTGGAAGCAGAAGCACGAGGAGTCTCAGGCGTTGCTGGAT
GCCTCTCAGAAGGAAGTTCAGGCTCTCAGTACAGAGCTCCTCAAGCTCAAGAACACCTAT
GAGGAGAGCATCGTGGGCCAGGAGACTCTCAGGAGGGAGAACAAGAACCTGCAAGAAGAG
ATTTCTAATCTCACAAACCAGGTTAGAGAAGGGACCAAGAACTTGACTGAAATGGAAAAG
GTCAAGAAACTGATTGAAGAAGAGAAGACAGAGGTCCAGGTGACACTGGAAGAAACAGAG
GGAGCCCTGGAACGTAATGAAAGCAAGATTCTTCATTTCCAGCTTGAACTCTTGGAAGCT
AAAGCAGAACTTGAAAGAAAGCTTTCAGAGAAAGATGAAGAAATAGAAAATTTTAGGAGG
AAGCAGCAGTGTACCATTGACTCCCTGCAGTCAAGTCTGGATTCTGAAGCTAAGAGTAGA
ATTGAGGTTACCCGGCTGAAGAAGAAGATGGAAGAGGACCTGAATGAGATGGAACTCCAG
CTTAGCTGTGCCAACCGACAGGTGTCAGAAGCAACCAAATCCCTGGGCCAGCTTCAGATT
CAAATCAAGGACCTTCAAATGCAGCTGGATGACAGCACACAACTGAACAGTGATCTGAAG
GAGCAGGTTGCTGTGGCTGAGCGGCGCAACTCTCTACTGCAGTCTGAACTAGAGGATCTA
AGGTCCCTGCAAGAGCAGACAGAGCGAGGCCGCAGGCTGTCAGAAGAAGAGCTCCTGGAA
GCAACAGAAAGAATCAATCTTTTCTATACCCAGAACACAAGCCTGCTCAGCCAGAAGAAG
AAACTGGAGGCTGATGTTGCCCGGATGCAGAAAGAAGCTGAAGAGGTGGTGCAGGAGTGT
CAAAATGCAGAAGAGAAGGCCAAGAAGGCAGCCATTGAGGCAGCCAACTTGTCCGAAGAA
CTGAAGAAGAAGCAAGACACCATTGCCCACTTGGAAAGGACACGAGAAAATATGGAGCAG
ACAATTACAGACTTACAGAAAAGGCTGGCTGAAGCTGAACAGATGGCCCTGATGGGGAGT
AGAAAGCAAATCCAGAAACTGGAAAGTAGGGTTCGTGAACTGGAAGGTGAACTTGAGGGT
GAAATCCGTCGCAGTGCCGAGGCCCAGAGGGGAGCCCGGAGACTTGAGCGATGCATCAAA
GAGCTGACTTATCAGGCAGAGGAAGACAAGAAGAACCTGAGCAGGATGCAAACTCAGATG
GATAAACTTCAGCTAAAAGTGCAAAATTACAAGCAGCAAGTCGAGGTGGCGGAAACACAA
GCCAATCAGTACCTTTCCAAGTATAAGAAACAGCAACATGAGTTGAATGAAGTGAAGGAA
AGGGCAGAGGTGGCAGAATCTCAAGTCAATAAACTCAAAATTAAGGCAAGAGAGTTTGGG
AAAAAGGTTCAAGAAGAA
Protein sequence predicted based on nucleotide sequence
MVESCLLTFRAFFWWIALIKMDLSDLGEAAAFLRRSEAELLLLQATALDGKKKCWIPDGE NAYIEAEVKGSEDDGTVIVETADGESLSIKEDKIQQMNPPEFEMIEDMAMLTHLNEASVL HTLKRRYGQWMIYTYSGLFCVTINPYKWLPVYQKEVMAAYKGKRRSEAPPHIFAVANNAF QDMLHNRENQSILFTGESGAGKTVNSKHIIQYFATIAAMIESRKKQGALEDQIMQANTIL EAFGNAKTLRNDNSSRFGKFIRMHFGARGMLSSVDIDIYLLEKSRVIFQQAGERNYHIFY QILSGQKELHDLLLVSANPSDFHFCSCGAVTVESLDDAEELLATEQAMDILGFLPDEKYG CYKLTGAIMHFGNMKFKQKPREEQLEADGTENADKAAFLMGINSSELVKCLIHPRIKVGN EYVTRGQTIEQVTCAVGALSKSMYERMFKWLVARINRALDAKLSRQFFIGILDITGFEIL EYNSLEQLCINFTNEKLQQFFNWHMFVLEQEEYKKESIEWVSIGFGLDLQACIDLIEKPM GILSILEEECMFPKATDLTFKTKLFDNHFGKSVHLQKPKPDKKKFEAHFELVHYAGVVPY NISGWLEKNKDLLNETVVAVFQKSSNRLLASLFENYMSTDSAIPFGEKKRKKGASFQTVA SLHKENLNKLMTNLKSTAPHFVRCINPNVNKIPGILDPYLVLQQLRCNGVLEGTRICREG FPNRLQYADFKQRYCILNPRTFPKSKFVSSRKAAEELLGSLEIDHTQYRFGITKVFFKAG FLGQLEAIRDERLSKVFTLFQARAQGKLMRIKFQKILEERDALILIQWNIRAFMAVKNWP WMRLFFKIKPLVKSSEVGEEVAGLKEECAQLQKALEKSEFQREELKAKQVSLTQEKNDLI LQLQAEQETLANVEEQCEWLIKSKIQLEARVKELSERVEEEEEINSELTARGRKLEDECF ELKKEIDDLETMLVKSEKEKRTTEHKVKNLTEEVEFLNEDISKLNRAAKVVQEAHQQTLD DLHMEEEKLSSLSKANLKLEQQVDELEGALEQERKARMNCERELHKLEGNLKLNRESMEN LESSQRHLAEELRKKELELSQMNSKVENEKGLVAQLQKTVKELQTQIKDLKEKLEAERTT RAKMERERADLTQDLADLNERLEEVGGSSLAQLEITKKQETKFQKLHRDMEEATLHFETT SASLKKRHADSLAELEGQVENLQQVKQKLEKDKSDLQLEVDDLLTRVEQMTRAKANAEKL CTLYEERLHEATAKLDKVTQLANDLAAQKTKLWSESGEFLRRLEEKEALINQLSREKSNF TRQIEDLRGQLEKETKSQSALAHALQKAQRDCDLLREQYEEEQEVKAELHRTLSKVNAEM VQWRMKYENNVIQRTEDLEDAKKELAIRLQEAAEAMGVANARNASLERARHQLQLELGDA LSDLGKVRSAAARLDQKQLQSGKALADWKQKHEESQALLDASQKEVQALSTELLKLKNTY EESIVGQETLRRENKNLQEEISNLTNQVREGTKNLTEMEKVKKLIEEEKTEVQVTLEETE GALERNESKILHFQLELLEAKAELERKLSEKDEEIENFRRKQQCTIDSLQSSLDSEAKSR IEVTRLKKKMEEDLNEMELQLSCANRQVSEATKSLGQLQIQIKDLQMQLDDSTQLNSDLK EQVAVAERRNSLLQSELEDLRSLQEQTERGRRLSEEELLEATERINLFYTQNTSLLSQKK KLEADVARMQKEAEEVVQECQNAEEKAKKAAIEAANLSEELKKKQDTIAHLERTRENMEQ TITDLQKRLAEAEQMALMGSRKQIQKLESRVRELEGELEGEIRRSAEAQRGARRLERCIK ELTYQAEEDKKNLSRMQTQMDKLQLKVQNYKQQVEVAETQANQYLSKYKKQQHELNEVKE RAEVAESQVNKLKIKAREFGKKVQEE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
