Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746375
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6966nts
Open reading frame: 1 to 6966
ATGCAGTCCGGGCCGCGGCCCCCACTTCCAGCCCCCGGCCTGGCCTTGGCTTTGACCCTG
ACTATGTTGGCCAGACTTGCATCCGCGGCTTCCTTCTTCGGTGAGAACCACCTGGAGGTG
CCTGTGGCCACGGCTCTGACCGACATAGACCTGCAGCTGCAGTTCTCCACGTCCCAGCCC
GAAGCCCTCCTGCTCCTGGCAGCAGGCCCAGCTGACCACCTCCTGTTGCAGCTCTACTCT
GGACGGCTTCAGGTCAGACTTGTTCTGGGCCAGGAGGAGCTGAGGCTGCAGACTCCAGCA
GAGACGCTGCTGAGTGACTCCATCCCCCACACTGTGGTGCTGACTGTCGTAGAGGGTTGG
GCCACGTTGTCAGTGGATGGGTTTCTGAACGCCTCCTCAGCAGTCCCCGGAGCCCCCCTA
GAGGTCCCCTATGGGCTCTTTGTTGGGGGCACTGGGACCCTTGGCCTGCCCTACCTGAGG
GGAACCAGCCGACCCCTGAGGGGTTGCCTCCATGCAGCCACCCTCAATGGCCGGAGCCTC
CTCCGGCCTCTGACCCCCGATGTGCATGAGGGCTGTGCTGAAGAGTTTTCTGCCAGTGAT
GATGTGGCCCTGGGCTTCTCTGGGCCCCACTCTCTTGCTGCCTTCCCTGCCTGGGGCACT
CAGGACGAAGGAACCCTAGAGTTTACACTCACCACACAGAGCCGGCAGGCACCCTTGGCC
TTCCAGGCAGGGGGACGGCGTGGGGACTTCATCTATGTGGACATATTTGAGGGCCACCTT
CGGGCCGTCGTGGAGAAGGGCCAGGGTACCGTATTGCTCCACAACAGTGTGCCCGTGGCC
GATGGGCAGCCCCATGAGGTCAGTGTCCACATCAATGCTCACCGGCTGGAAATCTCCGTG
GACCAGTACCCTACGCATACTTCGAACCGAGGAGTCCTCAGCTACCTGGAGCCACGGGGC
AGTCTCCTTCTCGGGGGCCTGGATGCAGAGGCCTCTCGTCACCTCCAGGAACACCGCCTG
GGCCTGACACCAGAGGCCACCAACGCCTCCCTGCTCGGCTGCATGGAAGACCTCAGCGTC
AATGGCCAGAGGCGGGGGCTGCGGGAAGCTTTACTGACGCGCAACATGGCAGCCGGCTGC
AGGCTGGAGGAGGAGGAGTATGAGGACGATGCCTATGGACATTATGAAGCTTTCTCCACC
CTGGCCCCTGAGGCTTGGCCAGCCATGGAGTTGCCTGAGCCATGCGTTCCTGAGCCAGGG
CTGCCTCCTGTCTTTGCCAATTTCACCCAGCTGCTGACTATCAGCCCACTGGTGGTGGCC
GAAGGGGGCACAGCCTGGCTTGAGTGGAGGCATGTGCAGCCCACGCTGGACCTGATGGAG
GCTGAGCTGCGCAAATCCCAGGTGCTGTTCAGCGTGACACGAGGGGCACGCCATGGCGAG
CTCGAGCTGGACATCCCGGGAGCCCAGGCACGAAAAATGTTCACCCTCCTGGACGTGGTG
AACCGCAAGGCCCGCTTCATCCACGATGGCTCTGAGGACACTTCCGACCAGCTGGTGCTC
GAGGTGTCGGTGACGGCTCGGGTGCCCATGCCCTCATGCCTTCGGAGGGGCCAAACATAC
CTCCTGCCTATCCAGGTCAACCCTGTCAATGACCCACCCCACATCATCTTCCCACATGGT
AGCCTCATGGTGATCCTGGAACACACGCAGAAGCCGCTGGGGCCTGAGGTGTTCCAGGCC
TATGACCCGGACTCAGCCTGTGAGGGCCTCACCTTCCAGGTCCTTGGCACATCCTCAGGC
CTCCCCGTGGAGCGCCGCGACCAGCCTGGGGAGCCAGCAACCGAGTTCTCTTGCCGGGAG
TTGGAGGCCGGCAGCCTAGTCTATGTCCACCGGGGTGGTCCTGCACAGGACCTGACGTTC
CGGGTCAGCGATGGACTGCAGGCCAGCCCCCCGGCTACGCTGAAAGTGGTGGCCATCAGG
CCGGCCATACAGATCCACCGCAGCACCGGGTTGAGATTGGCCCAAGGCTCTGCCATGCCC
ATCTTGCCCGCTAACCTGTCGGTGGAGACAAATGCCGTGGGGCAGGATGTGAGCGTGCTG
TTCCGCGTCACTGGGGCCCTGCAGTTTGGGGAGCTGCAGAAGCAGGGGGCTGGTGGGGTG
GAGGGTGCTGAGTGGTGGGCCACACAGGCGTTCCACCAGCGGGATGTGGAGCAGGGCCGC
GTGAGGTACCTGAGCACTGACCCACAGCACCACGCTTACGACACCGTGGAGAACCTGGCC
CTGGAGGTTCAGGTGGGACAGGAGATCCTGAGCAATCTGTCCTTCCCAGTGACCATCCAG
CGAGCCACCGTGTGGATGCTGCGGCTGGAGCCACTGCACACTCAGAACACCCAGCAGGAA
ACCCTGACCACAGCCCACCTGGAGGCCACCCTGGAGGAGGCAGGCCCAAGCCCCCCAACC
TTCCATTATGAGGTGGTTCAGGCTCCCAGGAAAGGCAACCTTCAACTACAGGGCACAAGG
CTGTCGGATGGACAGGGCTTCACCCAGGATGACATACAGGCTGGCCGGGTGACCTATGGG
GCCACAGCACGTGCCTCAGAGGCAGTCGAGGACACCTTCCGATTCCGTGTCACAGCTCCA
CCATATTTCTCCCCACTCTATACCTTCCCCATCCACATTGGTGGTGACCCAGATGCGCCT
GTCCTCACCAATGTCCTCCTCGTGGTGCCTGAGGGTGGTGAGGGTGTCCTCTCTGCTGAC
CACCTCTTTGTCAAGAGTCTCAACAGTGCCAGCTACCTCTATGAGGTCATGGAGCGGCCC
CGCCATGGGAGGTTGGCTTGGCGGGGGACACAGGACAAGACCACTATGGTGACATCCTTC
ACCAATGAAGACCTGTTGCGTGGCCGGCTGGTCTACCAGCATGATGACTCCGAGACAACA
GAAGATGATATCCCATTTGTTGCTACCCGCCAGGGCGAGAGCAGTGGTGACATGGCCTGG
GAGGAGGTACGGGGTGTCTTCCGAGTGGCCATCCAGCCCGTGAATGACCACGCCCCTGTG
CAGACCATCAGCCGGATCTTCCACGTGGCCCGGGGTGGGCGGCGGCTGCTCACTACAGAC
GACGTCGCCTTCAGCGATGCTGACTCGGGCTTTGCTGACGCACAGCTGGTGCTTACCCGA
AAGGACCTCCTCTTTGGCAGTATCGTGGCCGTAGATGAGCCCACGAGGCCCATCTACCGC
TTCACACAGGAGGACCTCAGGAAGAGGCGAGTACTGTTCGTGCACTCAGGGGCTGACCGT
GGATGGATCCAGCTACAGGTGTCCGACGGGCAACACCAGGCCACTGCGCTGCTGGAGGTG
CAGGCCTCGGAACCCTACCTCCGTGTGGCCAACGGCTCCTCCCTTGTGGTCCCTCAAGGA
GGCCAGGGCACCATCGACACGGCCGTGCTCCACCTGGACACCAACCTCGACATCCGCAGC
GGGGATGAGGTCCACTACCACGTCACAGCCGGCCCTCGCTGGGGACAGCTAGTCCGGGCT
GGTCAGCCAGCCACAGCCTTCTCCCAGCAGGACCTGCTGGATGGGGCCGTTCTCTATAGT
CACAATGGCAGCCTAAGCCCCCGCGACACCATGGCTTTCTCCGTGGAAGCAGGGCCAGTG
CACACGGATGCCACCCTACAAGTGACCATTGCCCTAGAGGGCCCACTGGCCCCACTGAAG
CTGGTCCGCCACAAGAAGATCTACGTCTTCCAGGGGGAGGCAGCTGAGATCAGACGGGAC
CAACTGGAGGCAGCCCAGGAGGCCGTGCCTCCTGCAGACATCGTATTCTCAGTGAAGAGC
CCACCGAGTGCCGGCTACCTGGTGATGGTGTCCCGTGGCGCCTTGGCAGATGAGCCGCCC
AGCCTGGACCCTGTGCAGAGCTTCTCTCAGGAGGCAGTGGACACAGGCAGGGTCCTGTAC
CTGCACTCCCGACCTGAGGCCTGGAGCGATGCCTTCTCACTGGATGTGGCCTCAGGCTTG
GGTGCTCCCCTCGAGGGCGTCCTTGTGGAGCTGGAGGTGCTGCCCGCTGCCATCCCACTA
GAGGCGCAAAACTTCAGCGTCCCTGAGGGTGGCAGTCTCACCCTGGCCCCCCCACTGCTC
CGTGTCTCCGGGCCCTACTTCCCCACTCTCCTGGGCCTCAGCCTGCAGGTTCTGGAGCCA
CCCCAGCATGGAGCCCTGCAGAAGGAGGACGGACCTCAAGCTAGGACCCTCAGCGCCTTC
TCCTGGAGAATGGTGGAAGAGCAGCTGATCCGCTACGTGCATGACGGGAGCGAGACACTG
ACAGACAGTTTTGTCCTGATGGCTAATGCCTCCGAGATGGATCGCCAGAGCCATCCTGTG
GCCTTCACTGTCACTGTCCTGCCCGTCAATGACCAACCCCCCATCCTCACTACAAACACA
GGCCTGCAGATGTGGGAGGGGGCCACTGCGCCCATCCCTGCGGAGGCACTGCGGAGCACG
GACGGCGACTCTGGGTCTGAGGATCTGGTCTACACCATCGAGCAGCCCAGCAACGGGCGG
GTAGTGCTGCGAGGGGCGCCGGGCACTGAGGTGCGCAGCTTCACGCAAGCTCAGCTGGAC
GGCGGGCTAGTGCTGTTCTCACACAGAGGAACCCTGGATGGAGGCTTCCGCTTTCGCCTC
TCTGACGGCGAGCACACTTCCCCCGGACACTTCTTCCGCGTGACGGCCCAGAAGCAAGTG
CTCCTCTCGCTGAAGGGCAGCCAGACACTGACTGTCTGCCCAGGGTCCGTGCAGCCACTT
AGCAGTCAGACCCTCAGGGCCAGCTCCAGCGCAGGCACTGACCCCCAACTACTGCTCTAC
CGGGTGGTGCGGGGCCCCCAGTTAGGCCGGCTGTTCCACGCCCAGCAGGACAGCACAGGG
GAGGCCCTTGTGAACTTCACTCAGGCAGAGGTCTACGCTGGGAATATTCTGTATGAGCAT
GAGATGCCCCCCGAGCCCTTTTGGGAGGCCCATGATACCCTAGAGCTCCAGCTGTCCTCG
CCGCCTGCCCGCGACGTGGCCGCCACCCTTGCTGTGGCTGTGTCTTTTGAGGCTGCCTGT
CCACAGCGCCCCAGCCACCTCTGGAAGAACAAAGGTCTGTGGGTCCCCGAGGGCCAGCGG
GCCAGGATCACCGTGGCTGCTCTGGATGCCTCCAATCTCTTGGCCAGCGTTCCATCACCC
CAGCGCTCAGAGCATGATGTGCTCTTCCAGGTCACACAGTTCCCCAGCCGGGGCCAGCTG
TTGGTGTCCGAGGAGCCCCTCCACGCTGGGCAGCCCCACTTCCTGCAGTCCCAGCTTGCT
GCAGGGCAGCTAGTGTATGCCCACGGCGGTGGGGGCACCCAGCAGGATGGCTTCCACTTT
CGTGCCCACCTCCAGGGACCAGCAGGGGCCTCCGTGGCTGGACCCCAAACCTCAGAGGCC
TTTGCCATCACGGTGAGGGATGTAAATGAGCGGCCACCTCAGCCACAGGCCTCTGTGCCA
CTCCGGCTCACCCGAGGCTCTCGAGCCCCCATCTCCCGGGCCCAGCTGAGTGTGGTGGAC
CCAGACTCAGCTCCTGGGGAGATTGAGTACGAGGTCCAGCGGGCCCCCCACAACGGCTTC
CTTAGCCTGGTGGGTGGTGGCTTGGGGCCCGTGACCCGGTTCACACAAGCCGATGTGGAT
TCAGGGCGGCTGGCCTTCGTGGCCAACGGGAGCAGCGTGGCAGGCATCTTCCAGCTGAGC
ATGTCTGATGGGGCAAGCCCACCCCTGCCCATGTCCCTGGCTGTGGACATCCTACCATCC
GCCATCGAGGTGCAGCTGCGGGCACCCCTGGAGGTGCCCCAAGCTTTGGGGCGCTCCTCA
CTGAGCCAGCAGCAGCTTCGGGTGGTTTCAGATCGGGAGGAGCCAGAGGCAGCATACCGC
CTCATCCAGGGACCCCAGTATGGGCATCTCCTGGTGGGCGGGCGGCCCACCTCGGCCTTC
AGCCAATTCCAGATAGACCAGGGCGAGGTGGTCTTTGCCTTCACCAACTTCTCCTCTTCT
CATGACCACTTCAGAGTCCTGGCACTGGCTAGGGGTGTCAATGCATCAGCCGTAGTGAAC
GTCACTGTGAGGGCTCTGCTGCATGTGTGGGCAGGAGGGCCATGGCCCCAGGGTGCCACC
CTGCGCTTAGACCCCACCGTCCTAGATGCTGGCGAGCTGGCCAACCGCACAGGCAGTGTG
CCTCGCTTCCGCCTCTTGGAGGGACCTCGGCATGGCCGAGTGGTCCGGGTTCCCAGAGCC
AGGACAGAGCCTGGGGGCAGCCAGCTGGTGGAGCAGTTCACTCAGCAGGACCTTGAGGAC
GGGAGGCTCGGACTGGAGGTGGGCAGACCAGAAGGGAGGGCTCCCGGCCCCGCAGGAGAC
AGTCTCACTCTGGAGTTATGGGCACAGGGCGTCCCCCCTGCTGTGGCCTCCCTGGACTTT
GCCACTGAGCCTTACAATGCTGCCCGGCCCTACAGTGTCGCCCTGCTCAGTGTCCCCGAG
GCCGCCCGGACAGAAGCTGGGAAGCCAGAGAGCAGCACCCCTACAGGCGAGCCAGGACCC
ATGGCATCCAGCCCTGAGCCCGCTGTGGCCAAGGGAGGCTTTCTGAGCTTCCTTGAGGCC
AACATGTTCAGCGTCATCATCCCCATGTGCCTGGTACTTCTCCTCCTGGCGCTCATCCTG
CCCCTGCTCTTCTACCTCCGAAAACGCAACAAGACGGGCAAGCATGACGTCCAGGTCCTG
ACTGCCAAGCCCCGCAACGGCCTGGCTGGTGACACCGAAACCTTTCGCAAGGTGGAGCCA
GGTCAGGCCATCCCGCTCACAGCTGTGCCTGGCCAGGGGCCCCCTCCAGGCGGACAGCCT
GACCCAGAGCTGCTTCAGTTCTGCCGGACACCCAACCCTGCCCTTAAGAATGGCCAGTAC
TGGGTG
Protein sequence predicted based on nucleotide sequence
MQSGPRPPLPAPGLALALTLTMLARLASAASFFGENHLEVPVATALTDIDLQLQFSTSQP EALLLLAAGPADHLLLQLYSGRLQVRLVLGQEELRLQTPAETLLSDSIPHTVVLTVVEGW ATLSVDGFLNASSAVPGAPLEVPYGLFVGGTGTLGLPYLRGTSRPLRGCLHAATLNGRSL LRPLTPDVHEGCAEEFSASDDVALGFSGPHSLAAFPAWGTQDEGTLEFTLTTQSRQAPLA FQAGGRRGDFIYVDIFEGHLRAVVEKGQGTVLLHNSVPVADGQPHEVSVHINAHRLEISV DQYPTHTSNRGVLSYLEPRGSLLLGGLDAEASRHLQEHRLGLTPEATNASLLGCMEDLSV NGQRRGLREALLTRNMAAGCRLEEEEYEDDAYGHYEAFSTLAPEAWPAMELPEPCVPEPG LPPVFANFTQLLTISPLVVAEGGTAWLEWRHVQPTLDLMEAELRKSQVLFSVTRGARHGE LELDIPGAQARKMFTLLDVVNRKARFIHDGSEDTSDQLVLEVSVTARVPMPSCLRRGQTY LLPIQVNPVNDPPHIIFPHGSLMVILEHTQKPLGPEVFQAYDPDSACEGLTFQVLGTSSG LPVERRDQPGEPATEFSCRELEAGSLVYVHRGGPAQDLTFRVSDGLQASPPATLKVVAIR PAIQIHRSTGLRLAQGSAMPILPANLSVETNAVGQDVSVLFRVTGALQFGELQKQGAGGV EGAEWWATQAFHQRDVEQGRVRYLSTDPQHHAYDTVENLALEVQVGQEILSNLSFPVTIQ RATVWMLRLEPLHTQNTQQETLTTAHLEATLEEAGPSPPTFHYEVVQAPRKGNLQLQGTR LSDGQGFTQDDIQAGRVTYGATARASEAVEDTFRFRVTAPPYFSPLYTFPIHIGGDPDAP VLTNVLLVVPEGGEGVLSADHLFVKSLNSASYLYEVMERPRHGRLAWRGTQDKTTMVTSF TNEDLLRGRLVYQHDDSETTEDDIPFVATRQGESSGDMAWEEVRGVFRVAIQPVNDHAPV QTISRIFHVARGGRRLLTTDDVAFSDADSGFADAQLVLTRKDLLFGSIVAVDEPTRPIYR FTQEDLRKRRVLFVHSGADRGWIQLQVSDGQHQATALLEVQASEPYLRVANGSSLVVPQG GQGTIDTAVLHLDTNLDIRSGDEVHYHVTAGPRWGQLVRAGQPATAFSQQDLLDGAVLYS HNGSLSPRDTMAFSVEAGPVHTDATLQVTIALEGPLAPLKLVRHKKIYVFQGEAAEIRRD QLEAAQEAVPPADIVFSVKSPPSAGYLVMVSRGALADEPPSLDPVQSFSQEAVDTGRVLY LHSRPEAWSDAFSLDVASGLGAPLEGVLVELEVLPAAIPLEAQNFSVPEGGSLTLAPPLL RVSGPYFPTLLGLSLQVLEPPQHGALQKEDGPQARTLSAFSWRMVEEQLIRYVHDGSETL TDSFVLMANASEMDRQSHPVAFTVTVLPVNDQPPILTTNTGLQMWEGATAPIPAEALRST DGDSGSEDLVYTIEQPSNGRVVLRGAPGTEVRSFTQAQLDGGLVLFSHRGTLDGGFRFRL SDGEHTSPGHFFRVTAQKQVLLSLKGSQTLTVCPGSVQPLSSQTLRASSSAGTDPQLLLY RVVRGPQLGRLFHAQQDSTGEALVNFTQAEVYAGNILYEHEMPPEPFWEAHDTLELQLSS PPARDVAATLAVAVSFEAACPQRPSHLWKNKGLWVPEGQRARITVAALDASNLLASVPSP QRSEHDVLFQVTQFPSRGQLLVSEEPLHAGQPHFLQSQLAAGQLVYAHGGGGTQQDGFHF RAHLQGPAGASVAGPQTSEAFAITVRDVNERPPQPQASVPLRLTRGSRAPISRAQLSVVD PDSAPGEIEYEVQRAPHNGFLSLVGGGLGPVTRFTQADVDSGRLAFVANGSSVAGIFQLS MSDGASPPLPMSLAVDILPSAIEVQLRAPLEVPQALGRSSLSQQQLRVVSDREEPEAAYR LIQGPQYGHLLVGGRPTSAFSQFQIDQGEVVFAFTNFSSSHDHFRVLALARGVNASAVVN VTVRALLHVWAGGPWPQGATLRLDPTVLDAGELANRTGSVPRFRLLEGPRHGRVVRVPRA RTEPGGSQLVEQFTQQDLEDGRLGLEVGRPEGRAPGPAGDSLTLELWAQGVPPAVASLDF ATEPYNAARPYSVALLSVPEAARTEAGKPESSTPTGEPGPMASSPEPAVAKGGFLSFLEA NMFSVIIPMCLVLLLLALILPLLFYLRKRNKTGKHDVQVLTAKPRNGLAGDTETFRKVEP GQAIPLTAVPGQGPPPGGQPDPELLQFCRTPNPALKNGQYWV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
