Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746409
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5265nts
Open reading frame: 1 to 5265
ATGGCGTCGGAGCTAGAGCCCGAGGTGCAGGCCATCGACCGCAGCTTGCTCGAATGTTCG
GCCGAGGAGATTGCGGGGAAATGGCTGCAAGCAACTGACCTCACTAGGGAAGTGTACCAG
CATTTAGCCCACTATGTACCCAAAATCTACTGCAGGGGTCCCAACCCTTTTCCACAGAAA
GAAGACATGCTGGCACAGCATGTTTTGTTGGGACCAATGGAATGGTACTTATGTGGTGAA
GATCCAGCATTTGGATTTCCAAAACTTGAGCAAGCAAACAAACCTTCCCATCTTTGTGGT
CGTGTTTTTAAAGTAGGAGAGCCCACATATTCTTGCAGAGACTGTGCAGTTGATCCAACT
TGTGTTTTGTGCATGGAGTGCTTTTTGGGAAGCATTCACAGAGATCATCGATATAGGATG
ACAACATCAGGAGGTGGAGGTTTCTGTGACTGTGGTGATACTGAAGCCTGGAAAGAGGGT
CCTTACTGTCAAAAACATGAACTTAACACCTCTGAAATTGAGGAAGAAGAGGATCCTCTT
GTTCATTTATCAGAAGATGTGATAGCAAGAACCTATAACATTTTTGCCATTACGTTTCGG
TATGCAGTAGAAATATTAACCTGGGAAAAAGAAAGTGAATTGCCAGCAGATTTAGAGATG
GTAGAGAAGAGTGACACCTACTATTGCATGCTGTTTAATGATGAGGTTCACACATATGAA
CAAGTTATTTATACTCTTCAGAAAGCCGTTAACTGTACACAAAAAGAAGCTATTGGTTTT
GCAACTACCGTAGATCGAGATGGGCGCAGGTCTGTTCGATATGGAGATTTTCAGTATTGT
GAGCAAGCAAAATCAGTAATTGTGAGAAATACCAGTAGACAAACAAAGCCACTCAAAGTT
CAAGTTATGCATTCGTCGATCGTCGCACATCAGAATTTTGGTTTGAAACTTTTGTCTTGG
CTGGGAAGTATTATTGGATATTCAGATGGCCTTCGCCGGATTTTATGTCAAGTTGGTTTA
CAAGAAGGGCCAGATGGTGAAAACTCCTCTCTAGTGGACAGACTTATGCTTAGCGATTCC
AAACTATGGAAAGGCGCTAGAAGTGTATATCATCAGTTGTTCATGAGCAGTCTGCTTATG
GATTTGAAATACAAGAAACTATTTGCTGTTCGATTTGCAAAAAATTACCAGCAGTTGCAG
AGAGATTTTATGGAGGATGATCACGAGAGAGCAGTGTCGGTGACTGCTCTATCTGTCCAG
TTCTTCACCGCACCTACTCTGGCTCGAATGCTCATCACAGAAGAAAACTTAATGAGCATC
ATCATTAAGACTTTTATGGATCATTTGAGACATCGAGATGCCCAGGGCAGATTTCAGTTT
GAACGATACACTGCTTTACAAGCCTTCAAATTTAGGAGAGTACAGAGCCTTATTTTAGAT
CTCAAGTATGTGTTAATTAGCAAACCAACTGAATGGTCAGATGAGCTGAGGCAGAAGTTC
CTAGAAGGGTTTGATGCCTTTTTAGAATTACTAAAATGTATGCAGGGAATGGATCCAATT
ACACGTCAAGTAGGACAACATATTGAAATGGAACCAGAGTGGGAAGCAGCCTTCACACTT
CAAATGAAATTAACACATGTCATTTCAATGATGCAGGACTGGTGTGCTTCAGATGAAAAA
GTGTTAATCGAAGCTTACAAGAAATGTCTCGCTGTACTCATGCAGTGTCATGGTGGCTAT
ACTGATGGTGAACAGCCTATCACACTAAGTATTTGTGGCCATTCAGTGGAAACTATCAGG
TACTGCGTTTCCCAAGAAAAAGTGAGCATTCACCTCCCAGTTTCTCGGTTACTGGCAGGT
TTACATGTATTATTAAGCAAAAGTGAAGTAGCATATAAATTTCCAGAGCTCCTACCTCTC
AGTGAACTTAGCCCACCCATGTTGATAGAACACCCTCTGAGATGTCTTGTCCTGTGTGCC
CAGGTACATGCGGGAATGTGGAGAAGGAATGGGTTCTCTCTAGTAAACCAGATTTATTAC
TACCATAATGTGAAATGCAGACGTGAGATGTTTGACAAGGATGTAGTGATGCTTCAGACA
GGTGTCTCCATGATGGATCCAAATCATTTCCTGATGATCATGCTCAGCCGCTTTGAACTT
TATCAGATTTTCTCTACCCCAGACTATGGAAAAAGATTCAGTTCTGAGATTACCCATAAG
GATGTTGTTCAGCAGAACAATACTCTAATAGAAGAAATGCTATACCTCATTATCATGCTT
GTGGGAGAGAGATTTAGTCCTGGAGTTGGACAGGTAAATGCTACAGATGAAATCAAGCGA
GAGATTATCCATCAGTTGAGTATCAAGCCTATGGCTCATAGTGAATTGGTAAAATCTTTG
CCTGAAGATGAGAACAAGGAGACTGGCATGGAGAGTGTAATCGAAGCGGTTGCCCATTTC
AAGAAACCTGGATTAACAGGACGAGGCATGTATGAACTGAAACCAGAATGTGCCAAAGAG
TTCAACTTGTATTTCTATCACTTTTCCAGGGCAGAACAGTCCAAGGCAGAAGAAGCGCAA
CGGAAATTGAAAAGACAAAATAGAGAAGATACAGCCCTCCCACCTCCGGTGTTACCTCCA
TTCTGCCCTCTGTTTGCAAGCCTGGTTAACATTTTGCAGTCTGATGTCATGTTGTGCATT
ATGGGAACAATTCTGCAATGGGCCGTCGAACATAATGGATATGCCTGGTCAGAGTCCATG
CTGCAAAGGGTGTTACATTTAATTGGCATGGCACTACAAGAAGAAAAACAACATTTAGAG
AATGTCACGGAAGAGCATGTAGTAACATTTACCTTCACTCAGAAGATCAGCAAACCTGGT
GAAGCCCCAAAAAATTCTCCTAGCATACTAGCTATGCTCGAAACACTACAAAATGCTCCC
TACCTAGAAGTCCACAAAGACATGATTAGGTGGATACTGAAGACTTTTAATGCTGTTAAA
AAGATGAGGGAGAGTTCACCTACCAGCCCCGTGGCAGAGACAGAAGGCACCATAATGGAA
GAGAGTTCACGGGACAAAGACAAAGCTGAGAGGAAGAGAAAAGCTGAGATTGCCAGACTG
CGCAGAGAAAAGATCATGGCTCAGATGTCTGAAATGCAGCGGCATTTTATTGATGAAAAC
AAAGAACTCTTTCAGCAGACATTAGAACTCGATGCCTCAACATCTGCTGTTCTTGATCAT
AGCCCTGTGGCTTCAGATATGACACTTACAGCACTGGGCCCCGCACAAACTCAGGTTCCT
GAACAAAGACAATTCGTTACATGTATATTGTGTCAAGAGGAGCAAGAAGTTAAAGTGGAA
AGCAGGGCAATGGTCTTGGCAGCATTTGTTCAGAGATCAACTGTACTGTCAAAAAACAGA
AGTAAATTTATTCAAGATCCAGAAAAGTATGATCCATTATTCATGCACCCTGATCTGTCT
TGTGGAACACACACTAGTAGCTGTGGGCACATTATGCATGCCCATTGTTGGCAAAGGTAT
TTTGATTCCGTTCAAGCTAAAGAACAGCGAAGGCAACAGAGATTACGCTTACATACGAGC
TATGATGTAGAAAACGGAGAATTCCTTTGCCCCCTTTGTGAATGCTTGAGTAATACTGTT
ATTCCTCTGCTGCTTCCACCAAGAAATATTTTTAACAACAGGTTAAATTTTTCAGACCAA
CCAAACCTCACTCAGTGGATTAGAACAATATCTCAGCAAATAAAAGCATTACAGTTTCTT
AGGAAAGAAGAAAGTACTCCTAATAATGCCTCTACCAAGAATTCAGAAAATGTGGATGAA
TTACAGCTCCCTGAAGGGTTCAGACCTGATTTTCGTCCTAAGATCCCTTATTCTGAGAGC
ATAAAAGAAATGCTAACGACATTTGGAACAGCTACCTACAAGGTGGGACTGAAGGTTCAT
CCTAATGAAGAGGATCCTCGTGTTCCCATAATGTGTTGGGGTAGCTGCGCGTACACAATC
CAATCCATCGAAAGAATTTTGAGCGATGAAGATAAACCATTGTTTGGTCCTTTACCTTGC
AGGCTGGATGACTGTCTTAGGTCATTGACGAGATTCGCCGCTGCACACTGGACAGTGGCA
TCAGTTTCAGTGGTGCAAGGACATTTTTGTAAACTTTTTGCATCACTGGTGCCTAATGAC
AGCCATGAGGAACTCCCATGCATATTAGATATTGACATGTTCCATTTATTGGTGGGCTTG
GTCCTCGCATTTCCTGCGTTACAGTGTCAGGATTTTTCAGGGATCAGCCTTGGCACTGGA
GACTTGCACATTTTCCATCTGGTTACTATGGCACACATCATACAGATCTTACTTACTTCA
TGTACAGAAGAGAATGGCATGGATCAAGAAAATCCCCCCTGTGAAGAAGAATCAGCAGTG
CTGGCTTTATATAAAACACTTCACCAGTATACGGGAAGTGCCTTGAAAGAAATACCCTCT
GGCTGGCATCTGTGGAGGAGTGTCAGAGCTGGAATCATGCCTTTCCTGAAGTGTTCTGCT
TTATTTTTTCATTACTTAAATGGAGTTCCTTCTCCACCCGACATTCAAGTTCCTGGAACA
AGCCATTTTGAACATTTATGTAGCTATCTCTCTCTACCAAACAACCTTATTTGCCTTTTT
CAAGAAAATAGTGAGATAATGAATTCACTGATTGAAAGTTGGTGCCGTAACAGTGAAGTT
AAAAGGTATCTAGAAGGTGAAAGAGATGCTATAAGATATCCAAGAGAATCTAACAAATTA
ATAAACCTTCCAGAGGATTACAGCAGCCTCATTAATCAAGCATCCAATTTCTCGTGCCCG
AAATCAGGTGGCGATAAGAGCAGAGCCCCAACTCTGTGCCTCGTCTGCGGATCCCTGCTG
TGCTCACAGAGCTACTGCTGCCAGACTGAACTGGAAGGGGAGGATGTAGGAGCCTGCACA
GCTCACACCTACTCCTGTGGCTCTGGAGTGGGCATCTTCCTGAGAGTACGGGAATGTCAG
GTGCTATTTTTAGCTGGCAAAACCAAAGGCTGTTTTTATTCCCCTCCTTACCTTGATGAC
TATGGCGAGACAGACCAGGGACTCAGAAGGGGAAATCCTTTACATCTGTGCAAAGAGCGC
TTCAAGAAGATTCAGAAGCTCTGGCACCAACACAGTGTCACAGAGGAAATTGGACATGCA
CAGGAAGCCAATCAGACACTCGTTGGCATTGACTGGCAACATTTA
Protein sequence predicted based on nucleotide sequence
MASELEPEVQAIDRSLLECSAEEIAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQK EDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPT CVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPL VHLSEDVIARTYNIFAITFRYAVEILTWEKESELPADLEMVEKSDTYYCMLFNDEVHTYE QVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKV QVMHSSIVAHQNFGLKLLSWLGSIIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLSDS KLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYQQLQRDFMEDDHERAVSVTALSVQ FFTAPTLARMLITEENLMSIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLILD LKYVLISKPTEWSDELRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTL QMKLTHVISMMQDWCASDEKVLIEAYKKCLAVLMQCHGGYTDGEQPITLSICGHSVETIR YCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCA QVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDVVMLQTGVSMMDPNHFLMIMLSRFEL YQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMLVGERFSPGVGQVNATDEIKR EIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKE FNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCI MGTILQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLENVTEEHVVTFTFTQKISKPG EAPKNSPSILAMLETLQNAPYLEVHKDMIRWILKTFNAVKKMRESSPTSPVAETEGTIME ESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDH SPVASDMTLTALGPAQTQVPEQRQFVTCILCQEEQEVKVESRAMVLAAFVQRSTVLSKNR SKFIQDPEKYDPLFMHPDLSCGTHTSSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTS YDVENGEFLCPLCECLSNTVIPLLLPPRNIFNNRLNFSDQPNLTQWIRTISQQIKALQFL RKEESTPNNASTKNSENVDELQLPEGFRPDFRPKIPYSESIKEMLTTFGTATYKVGLKVH PNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVA SVSVVQGHFCKLFASLVPNDSHEELPCILDIDMFHLLVGLVLAFPALQCQDFSGISLGTG DLHIFHLVTMAHIIQILLTSCTEENGMDQENPPCEEESAVLALYKTLHQYTGSALKEIPS GWHLWRSVRAGIMPFLKCSALFFHYLNGVPSPPDIQVPGTSHFEHLCSYLSLPNNLICLF QENSEIMNSLIESWCRNSEVKRYLEGERDAIRYPRESNKLINLPEDYSSLINQASNFSCP KSGGDKSRAPTLCLVCGSLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQ VLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSVTEEIGHA QEANQTLVGIDWQHL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
