Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746410
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4731nts
Open reading frame: 1 to 4731
ATGGAGGCTGGCTCAGTGGTTCGAGCCATTTTTGACTTCTGCCCTAGCGTATCCGAAGAA
CTGCCGCTCTTTGTGGGAGATATTATTGAGGTCCTGGCAGTGGTGGATGAATTTTGGCTT
CTAGGCAAGAAGGAGGATGTTACAGGACAATTCCCCAGCAGTTTTGTGGAAATTGTGACC
ATTCCCAGTCTAAAAGAGGGAGAGCGGCTGTTTGTGTGCATTTGTGAATTCACATCCCAA
GAGTTGGATAATCTTCCCCTCCATCGAGGTGACCTGGTGATTCTGGATGGCATTCCCACT
GCAGGCTGGCTGCAGGGCCGAAGCTGCTGGGGCGCACGGGGCTTCTTCCCATCTTCATGT
GTCCGCGAGCTCTGCCTCTCCTCACAGAGCCGGCAGTGGCACTCCCAGAGCGCCCTGTTT
CAGATTCCGGAATATTCCATGGGACAAGCCCGGGCCCTAATGGGGCTTTCTGCTCAGCTG
GATGAAGAGCTGGACTTCAGGGAAGGGGATGTGATCACCATTATTGGAGTTCCTGAACCA
GGCTGGTTTGAAGGGGAGTTAGAGGGCCGAAGAGGCATTTTTCCAGAAGGTTTTGTAGAG
CTGTTGGGGCCCCTGAGGACTGTGGATGAGTCAGTAAGTTCTGGAAATCAAGATGACTGC
ATTGTTAATGGTGAAGTGGATACCCCTGTGGGAGAAGAAGAGATAGGGCCGGATGAGGAT
GAGGAGGAGCCAGGGACCTATGGGGTCGCTCTGTACAGATTCCAAGCCCTGGAGCCAAAT
GAGCTGGATTTCGAGGTCGGGGATAAAATCCGAATTCTGGCGACCTTGGAAGATGGCTGG
CTGGAAGGATCCCTGAAGGGCAGGACAGGCATCTTTCCCTACCGGTTTGTGAAATTATGT
CCTGACACACGGGTGGAGGAAACCATGGCTCTGCCCCAGGAAGGCAGCCTTGCCAGGATC
CCGGAAACTTCTTTGGATTGTTTGGAGAACACCTTAGGAGTAGAGGAACAAAGACATGAA
ACCAGTGACCATGAGGCCGAGGAACCTGACTGCATTATTTCTGAAGCACCCACTTCTCCC
CTCGGTCATCTGACTTCAGAGTATGACACAGACAGAAACTCTTATCAGGACGAGGACACC
GCAGGAGGGCCCCCGAGAAGCCCAGGCGTGGAGTGGGAAATGCCTCTTGCCACAGACTCT
CCCACATCTGACCCTACAGAAGTAGTCAATGGTATTTCCTCCCAACCTCAGGTCCCTTTT
CATCCCAACTTGCAGAAAAGCCAGTATTATTCTACAGTGGGAGGGAGCCACCCGCACTCA
GAACAGTACCCCGACCTTCTTCCCCTAGAAGCAAGGACTAGAGACTATGCCAGCCTACCT
CCCAAAAGAATGTATTCCCAGCTAAAAACTCTGCAGAAGCCAGTGCTCCCTCTTTACAGG
GGCTCTTCTGTTTCAGCCTCAAGGGTAGTCAAACCCAGGCAATCAAGTCCTCAGCTTCAC
AACCTAGCAAGTTATACTAAAAAGCACCACACGTCCAGTGTTTACTCCATCTCAGAGAGA
TTGGAGATGAAGCCTGGTCCGCAAGCCCAAGGGCTGGTTATGGAAGCAGCAACACATTCC
CAGGGAGACGGCAGCACTGACCTGGACTCGAAGCTGACACAACAGCTGATCGAGTTTGAG
AAGAGCTTGGCAGGGCCCGGCACAGAGCCCGATAAAATTTTACGCCACTTTTCCATCATG
GACTTTAACTCTGAGAAGGATATTGTCCGAGGTTCCTCAAAGTTAATCACCGAGCAGGAG
CTGCCGGAAAGGAGGAAGGCTCTGAGGCCACCGCCACCTCGTCCCTGTACTCCGGTATCC
ACTTCTCCCCATTTGCTGGTTGACCAGAACCTAAAACCTGCACCACCCTTGGTGGTGCGA
CCCTCTCGCCCAGCTCCCCTGCCTCCCTCAGCACAGCAGAGAACGAATGCGGTATCCCCC
AAGCTCCTATCTCGACACCGTCCTACCTGTGAAACCTTAGAAAAGGAGGGCCCTGGTCAT
ATGGGAAGGAGTCTGGACCAGACCTCCCCATGCCCCTTAGTGCTGGTGAGGATTGAGGAA
ATGGAGCGGGACCTGGATATGTACAGTAGAGCTCAAGAAGAGCTAAACCTCATGCTGGAG
GAGAAGCAGGATGAATCATCAAGAGCAGAAACCCTCGAGGATCTCAAGTTCTGTGAAAGT
AACATTGAAAGTTTGAATATGGAACTCCAGCAACTAAGAGAAATGACGCTCCTCTCCTCC
CAGTCCTCATCACTGGTGGCCCCCTCTGGGTCTGTGTCTGCCGAAAATCCAGAGCAGAGG
ATGCTGGAGAAGAGAGCCAAGGTCATAGAAGAACTTCTTCAGACAGAAAGAGACTACATT
CGGGATCTGGAAATGTGTATTGAGCGGATCATGGTACCCATGCAGCAGGCACAGGTACCA
AACATTGATTTTGAGGGACTTTTTGGAAATATGCAGATGGTGATTAAGGTTTCGAAGCAA
TTATTGGCTGCTCTGGAAATCAGCGATGCTGTAGGACCTGTGTTTCTTGGTCACCGGGAT
GAGCTTGAGGGAACATACAAGATTTACTGCCAGAATCATGATGAGGCCATTGCGCTGCTT
GAAATCTACGAGAAGGATGAGAAGATCCAGAAGCATCTTCAGGACTCCTTGGCAGATCTG
AAGAGCCTATACAACGAATGGGGATGCACAAATTATATTAACCTGGGCTCCTTCCTCATC
AAACCAGTACAGAGGGTAATGCGTTACCCGCTCTTGCTAATGGAGTTGCTGAATTCCACC
CCAGAATCCCACCCAGATAAAGTGCCTTTAACCAATGCAGTCCTGGCGGTCAAGGAAATC
AACGTTAACATTAATGAATATAAACGGCGAAAGGACCTGGTCCTCAAGTACCGTAAGGGT
GATGAAGATAGTCTTATGGAGAAAATTTCCAAACTGAACATCCACTCCATCATCAAGAAA
TCCAACCGAGTTAGCAGTCACCTGAAGCATCTGACTGGCTTTGCTCCTCAGATAAAAGAT
GAAGTATTTGAAGAAACAGAAAAAAACTTCCGAATGCAAGAAAGATTGATTAAGTCTTTT
ATCCGAGATCTGTCTCTCTACCTCCAGCACATCCGGGAGTCCGCATGTGTGAAAGTTGTG
GCTGCTGTGAGTATGTGGGATGTGTGCATGGAGAGAGGACACCGGGACCTGGAGCAGTTT
GAGCGCGTGCATCGCTACATCAGTGACCAGCTCTTCACAAACTTTAAGGAGAGGACAGAG
AGGCTTGTCATCTCCCCCTTAAATCAGTTACTGAGCATGTTTACAGGGCCCCATAAGCTG
GTACAGAAACGCTTTGACAAGCTCCTGGACTTCTATAACTGTACAGAACGGGCAGAAAAG
CTAAAGGACAAGAAGACCCTGGAGGAGTTGCAGTCGGCCCGGAACAACTATGAGGCCCTG
AATGCACAGCTGCTGGATGAGCTGCCCAAGTTCCACCAGTACGCTCAGGGCCTCTTCACC
AACTGTGTCCACGGCTATGCTGAAGCCCACTGTGACTTTGTGCACCAGGCTCTGGAGCAA
TTAAAGCCACTGCTCTCGTTACTCAAAGTGGCTGGCAGAGAGGGAAACCTTATTGCCATC
TTCCACGAAGAGCACAGCAGAGTTTTGCAGCAACTGCAGGTTTTTACCTTCTTCCCGGAG
TCTCTTCCAGCTACCAAGAAGCCATTTGAGAGGAAAACCATTGACCGCCAGTCTGCTCGA
AAGCCACTCCTGGGCCTGCCAAGTTACATGCTACAGTCAGAAGAACTCCGGGCCTCCCTG
CTGGCCAGGTATCCCCCTGAAAAACTCTTCCAGGCAGAGCGGAACTTCAATGCTGCTCAA
GACTTGGATGTCTCACTTCTGGAAGGTGACCTGGTGGGTGTGATTAAGAAAAAAGACCCC
ATGGGCAGCCAGAACCGCTGGCTGATTGACAATGGAGTCACCAAAGGCTTCGTGTACAGC
TCCTTCCTAAAGCCCTACAATCCTCGCCGCAGCCACTCTGATGCCTCCGTGGGTAGCCAC
TCCTCCACAGAGTCTGAGCACGGCAGTTCCTCCCCTAGGTTCCCACGCCAGAACAGCGGC
AGCACCCTCACCTTCAACCCCAGCAGCATGGCTGTATCCTTTACCTCGGGGTCTTGCCAG
AAGCAGCCTCAAGATGCATCTCCTCCGCCAAAAGAATGTGACCAAGGAACTCTCAGTGCA
TCCCTAAATCCGAGTAATTCAGAGAGTAGTCCTTCCAGATGCCCTTCAGACCCCGACTCT
ACCTCCCAGCCAAGGTCAGGGGACTCTGCAGATGTAGCTAGAGATGTAAAGCAACCCACT
GCCACCCCGAGAAGTTACCGGAACTTCAGGCATCCCGAAATAGTTGGCTACTCCGTACCA
GGACGAAATGGGCAAAGTCAAGACCTCGTCAAAGGATGTGCAAGAACAGCCCAGGCTCCG
GAAGACAGAAGTACCGAGCCAGATGGCAGTGAGGCAGAAGGCAACCAGGTCTATTTTGCT
GTCTACACCTTCAAGGCACGAAACCCAAATGAGCTGTCCGTGTCAGCCAATCAGAAACTC
AAGATCCTCGAGTTTAAAGATGTTACAGGAAATACAGAGTGGTGGTTAGCTGAGGTTAAC
GGGAAGAAGGGCTACGTTCCCTCCAATTATATCCGCAAAACCGAGTACACC
Protein sequence predicted based on nucleotide sequence
MEAGSVVRAIFDFCPSVSEELPLFVGDIIEVLAVVDEFWLLGKKEDVTGQFPSSFVEIVT IPSLKEGERLFVCICEFTSQELDNLPLHRGDLVILDGIPTAGWLQGRSCWGARGFFPSSC VRELCLSSQSRQWHSQSALFQIPEYSMGQARALMGLSAQLDEELDFREGDVITIIGVPEP GWFEGELEGRRGIFPEGFVELLGPLRTVDESVSSGNQDDCIVNGEVDTPVGEEEIGPDED EEEPGTYGVALYRFQALEPNELDFEVGDKIRILATLEDGWLEGSLKGRTGIFPYRFVKLC PDTRVEETMALPQEGSLARIPETSLDCLENTLGVEEQRHETSDHEAEEPDCIISEAPTSP LGHLTSEYDTDRNSYQDEDTAGGPPRSPGVEWEMPLATDSPTSDPTEVVNGISSQPQVPF HPNLQKSQYYSTVGGSHPHSEQYPDLLPLEARTRDYASLPPKRMYSQLKTLQKPVLPLYR GSSVSASRVVKPRQSSPQLHNLASYTKKHHTSSVYSISERLEMKPGPQAQGLVMEAATHS QGDGSTDLDSKLTQQLIEFEKSLAGPGTEPDKILRHFSIMDFNSEKDIVRGSSKLITEQE LPERRKALRPPPPRPCTPVSTSPHLLVDQNLKPAPPLVVRPSRPAPLPPSAQQRTNAVSP KLLSRHRPTCETLEKEGPGHMGRSLDQTSPCPLVLVRIEEMERDLDMYSRAQEELNLMLE EKQDESSRAETLEDLKFCESNIESLNMELQQLREMTLLSSQSSSLVAPSGSVSAENPEQR MLEKRAKVIEELLQTERDYIRDLEMCIERIMVPMQQAQVPNIDFEGLFGNMQMVIKVSKQ LLAALEISDAVGPVFLGHRDELEGTYKIYCQNHDEAIALLEIYEKDEKIQKHLQDSLADL KSLYNEWGCTNYINLGSFLIKPVQRVMRYPLLLMELLNSTPESHPDKVPLTNAVLAVKEI NVNINEYKRRKDLVLKYRKGDEDSLMEKISKLNIHSIIKKSNRVSSHLKHLTGFAPQIKD EVFEETEKNFRMQERLIKSFIRDLSLYLQHIRESACVKVVAAVSMWDVCMERGHRDLEQF ERVHRYISDQLFTNFKERTERLVISPLNQLLSMFTGPHKLVQKRFDKLLDFYNCTERAEK LKDKKTLEELQSARNNYEALNAQLLDELPKFHQYAQGLFTNCVHGYAEAHCDFVHQALEQ LKPLLSLLKVAGREGNLIAIFHEEHSRVLQQLQVFTFFPESLPATKKPFERKTIDRQSAR KPLLGLPSYMLQSEELRASLLARYPPEKLFQAERNFNAAQDLDVSLLEGDLVGVIKKKDP MGSQNRWLIDNGVTKGFVYSSFLKPYNPRRSHSDASVGSHSSTESEHGSSSPRFPRQNSG STLTFNPSSMAVSFTSGSCQKQPQDASPPPKECDQGTLSASLNPSNSESSPSRCPSDPDS TSQPRSGDSADVARDVKQPTATPRSYRNFRHPEIVGYSVPGRNGQSQDLVKGCARTAQAP EDRSTEPDGSEAEGNQVYFAVYTFKARNPNELSVSANQKLKILEFKDVTGNTEWWLAEVN GKKGYVPSNYIRKTEYT
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
