Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746436
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3105nts
Open reading frame: 1 to 3105
ATGGGTGGCCCGGCTGCGCCTAGGGGAGCCGGGAGATTGCGAGCGTTGTTACTTGCTTTG
GTGGTTGCTGGAATCCCTGCTGGAGCTTACAACCTGGACCCTCAGCGCCCCGTGCACTTC
CAGGGCCCCGCTGACTCGTTCTTCGGATACGCCGTTCTGGAGCATTTCCACGACAACACA
CGCTGGGTCTTAGTGGGCGCACCAAAGGCAGATTCCAAATACAGCCCTTCAGTGAAGTCT
CCTGGGGCTGTGTTTAAGTGCCGTGTTCACACCAACCCTGACCGGAGATGCACCGAACTG
GACATGGCTCGAGGGAAGAATCGGGGAACGTCCTGCGGAAAGACATGTCGGGAAGACCGC
GATGATGAGTGGATGGGGGTGAGCCTGGCTCGACAGCCCAAGGCTGATGGCCGTGTGTTG
GCCTGTGCTCATCGCTGGAAGAACATCTACTATGAAGCCGACCACATCCTACCCCATGGC
TTCTGCTACATCATCCCCTCCAACCTCCAGGCCAAAGGGAGGACGCTTATCCCTTGCTAT
GAAGAGTATAAGAAGAAGTACGGAGAGGAACACGGCTCCTGCCAGGCTGGGATAGCGGGC
TTCTTCACCGAGGAGCTCGTGGTGATGGGTGCTCCAGGGTCATTTTATTGGGCTGGAACC
ATCAAAGTGCTCAACCTTACGGACAACACCTATTTGAAACTGAACGACGAAGTGATCATG
AACAGGCGGTACACCTACCTGGGCTACGCAGTGACCGCTGGCCACTTCTCTCACCCGTCC
ACCATTGATGTGGTAGGAGGTGCCCCACAGGACAAAGGCATCGGCAAGGTTTATATTTTC
AGAGCTGACCGAAGATCCGGCACCTTAATTAAGATCTTTCAAGCATCAGGTAAAAAGATG
GGCTCTTACTTCGGCTCCTCCTTGTGCGCAGTTGACCTGAATGGGGACGGCCTCTCTGAC
CTGCTGGTGGGGGCCCCCATGTTCTCTGAGATCAGGGATGAGGGACAGGTCACTGTCTAC
ATCAACAGAGGAAATGGAGCCCTCGAGGAGCAGCTGGCTCTGACTGGGGATGGTGCCTAC
AATGCGCACTTTGGAGAGAGCATTGCCAGTCTGGACGATCTCGACAATGATGGGTTCCCA
GATGTGGCCATCGGTGCCCCCAAGGAGGATGACTTCGCAGGGGCGGTCTATATCTATCAT
GGTGATGCCGGTGGGATAGTCCCTCAGTACTCCATGAAACTGTCTGGGCAGAAGATAAAT
CCAGTGCTCCGGATGTTTGGTCAGTCCATATCGGGAGGCATTGATATGGATGGAAATGGC
TATCCTGATGTCACTGTTGGAGCTTTCATGTCCGACAGTGTGGTGCTTCTCAGGGCAAGG
CCTGTCATTACGGTGGATGTCTCCATCTTCCTCCCGGGCTCTATCAACATCACAGCGCCT
CAGTGTCACGACGGACAGCAGCCTGTGAACTGCTTGAACGTCACCACCTGTTTCAGCTTC
CATGGCAAACACGTGCCAGGAGAGATTGGCCTGAATTATGTTCTGATGGCTGACGTCGCT
AAAAAGGAGAAGGGCCAGATGCCCAGGGTCTACTTTGTGCTGCTCGGAGAGACAATGGGT
CAGGTCACAGAGAAGCTGCAGCTGACTTACATGGAGGAGACGTGTCGTCACTATGTGGCC
CATGTGAAGCGGAGGGTGCAGGACGTCATCAGCCCGATTGTGTTTGAAGCAGCCTACAGC
CTCAGTGAGCATGTGACTGGAGAGGAGGAGAGGGAACTGCCGCCTCTGACACCAGTTCTC
CGCTGGAAAAAGGGACAAAAGATTGCCCAAAAGAATCAGACTGTTTTTGAAAGGAATTGC
CGTTCAGAGGACTGTGCCGCAGACCTGCAGCTTCAGGGTAAACTGCTGCTCTCCAGTATG
GATGAGAAAACCCTGTATCTAGCTTTGGGGGCTGTGAAGAACATCTCCCTAAACATCTCT
ATCTCCAACCTCGGAGATGATGCCTATGATGCCAACGTCTCCTTCAATGTTTCCCGGGAG
CTCTTCTTCATCAACATGTGGCAGAAGGAGGAGATGGGCATCTCCTGTGAGCTGCTGGAA
TCGGACTTCCTCAAATGCAGCGTGGGATTTCCTTTCATGAGGTCAAAGTCAAAGTATGAA
TTCAGCGTGATCTTTGATACAAGCCACCTGTCTGGGGAAGAGGAAGTTCTCAGCTTCATT
GTTACTGCTCAGAGTGGCAACACGGAGCGCTCTGAATCCCTGCATGACAACACCCTCGTG
CTGATGGTGCCACTGATGCACGAGGTGGACACGTCCATCACCGGAATCATGTCTCCAACC
TCATTTGTATATGGCGAGTCCGTGGACGCAGCCAACTTCATTCAGCTGGATGACCTGGAG
TGTCACTTTCAGCCCATCAATATCACCCTTCAGGTCTACAACACTGGCCCAAGCACCCTT
CCCGGGTCATCAGTCAGTATCTCTTTCCCTAATCGACTCTCATCTGGTGGCGCAGAGATG
TTTCATGTCCAGGAAATGGTGGTTGGCCAAGAGAAGGGAAACTGCTCTTTCCAGAAAAAC
CCAACTCCCTGCATCATCCCTCAAGAACAAGAAAATATCTTCCACACCATCTTTGCTTTT
TTCACAAAGTCTGGAAGAAAAGTCTTGGACTGTGAAAAACCAGGCATTTCTTGCCTGACA
GCACACTGTAACTTTAGTGCTCTTGCTAAAGAAGAAAGTCGTACTATAGACATCTACATG
CTGCTGAACACAGAAATACTGAAAAAGGACAGTTCGTCTGTCATCCAGTTCATGTCTCGC
GCCAAGGTGAAGGTGGATCCTGCCCTAAGGGTGGTGGAAATAGCTCATGGGAACCCAGAA
GAGGTGACGGTGGTCTTCGAGGCCCTTCACAATCTCGAGCCCCGTGGCTACGTCGTGGGG
TGGATCATCGCCATCAGTTTGTTGGTGGGAATCCTCATCTTCCTGCTGCTGGCCGTGCTG
CTCTGGAAGATGGGCTTCTTTCGCCGAAGGTACAAAGAAATTATCGAAGCTGAGAAGAAC
CGGAAAGAGAATGAAGACAGTTGGGACTGGGTCCAGAAAAATCAG
Protein sequence predicted based on nucleotide sequence
MGGPAAPRGAGRLRALLLALVVAGIPAGAYNLDPQRPVHFQGPADSFFGYAVLEHFHDNT RWVLVGAPKADSKYSPSVKSPGAVFKCRVHTNPDRRCTELDMARGKNRGTSCGKTCREDR DDEWMGVSLARQPKADGRVLACAHRWKNIYYEADHILPHGFCYIIPSNLQAKGRTLIPCY EEYKKKYGEEHGSCQAGIAGFFTEELVVMGAPGSFYWAGTIKVLNLTDNTYLKLNDEVIM NRRYTYLGYAVTAGHFSHPSTIDVVGGAPQDKGIGKVYIFRADRRSGTLIKIFQASGKKM GSYFGSSLCAVDLNGDGLSDLLVGAPMFSEIRDEGQVTVYINRGNGALEEQLALTGDGAY NAHFGESIASLDDLDNDGFPDVAIGAPKEDDFAGAVYIYHGDAGGIVPQYSMKLSGQKIN PVLRMFGQSISGGIDMDGNGYPDVTVGAFMSDSVVLLRARPVITVDVSIFLPGSINITAP QCHDGQQPVNCLNVTTCFSFHGKHVPGEIGLNYVLMADVAKKEKGQMPRVYFVLLGETMG QVTEKLQLTYMEETCRHYVAHVKRRVQDVISPIVFEAAYSLSEHVTGEEERELPPLTPVL RWKKGQKIAQKNQTVFERNCRSEDCAADLQLQGKLLLSSMDEKTLYLALGAVKNISLNIS ISNLGDDAYDANVSFNVSRELFFINMWQKEEMGISCELLESDFLKCSVGFPFMRSKSKYE FSVIFDTSHLSGEEEVLSFIVTAQSGNTERSESLHDNTLVLMVPLMHEVDTSITGIMSPT SFVYGESVDAANFIQLDDLECHFQPINITLQVYNTGPSTLPGSSVSISFPNRLSSGGAEM FHVQEMVVGQEKGNCSFQKNPTPCIIPQEQENIFHTIFAFFTKSGRKVLDCEKPGISCLT AHCNFSALAKEESRTIDIYMLLNTEILKKDSSSVIQFMSRAKVKVDPALRVVEIAHGNPE EVTVVFEALHNLEPRGYVVGWIIAISLLVGILIFLLLAVLLWKMGFFRRRYKEIIEAEKN RKENEDSWDWVQKNQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
