Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746461
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4611nts
Open reading frame: 1 to 4611
ATGGGGGAGAAGCTGGAGCTGAGACTGAAGTCGCCCGTGGGGGCTGAGCCCGCCGTCTAC
CCGTGGCCGCTGCCGGTCTACGATAAACATCACGATGCCGCTCATGAAATCATCGAAACC
ATCCGATGGGTCTGTGAAGAAATCCCGGATCTCAAGCTCGCTATGGAGAATTACGTTTTA
ATTGACTATGACACCAAAAGTTTCGAGAGCATGCAGAGGCTCTGCGACAAGTACAACCGT
GCCATCGACAGCATCCACCAGCTGTGGAAGGGCACCACGCAGCCTATGAAGCTGAACACG
CGGCCGTCCACTGGACTCCTCCGCCATATCCTGCAGCAGGTCTACAACCACTCGGTGACC
GACCCCGAGAAGCTCAACAACTACGAGCCCTTCTCCCCCGAGGTGTACGGCGAGACATCC
TTCGACCTGGTGGCCCAGATGATTGATGAGATCAAGATGACCGACGACGACCTGTTTGTG
GACTTGGGGAGCGGTGTGGGCCAGGTCGTGCTCCAGGTTGCTGCTGCCACCAACTGCAAA
CATCACTATGGCGTCGAGAAAGCAGACATCCCGGCCAAGTATGCGGAGACAATGGACCGC
GAGTTCAGGAAGTGGATGAAATGGTATGGAAAAAAGCATGCAGAATACACATTGGAGAGA
GGCGATTTCCTCTCAGAAGAGTGGAGGGAGCGAATCGCCAACACCAGTGTTATATTTGTG
AATAATTTTGCCTTTGGTCCTGAGGTGGATCACCAGCTGAAGGAGCGGTTTGCAAACATG
AAGGAAGGTGGCAGAATCGTGTCCTCGAAACCCTTTGCACCTCTGAACTTCAGAATAAAC
AGTAGAAACTTGAGTGACATCGGCACCATCATGCGCGTGGTGGAGCTCTCGCCCCTGAAG
GGCTCGGTGTCGTGGACGGGGAAGCCAGTCTCCTACTACCTGCACACTATCGACCGCACC
ATACTTGAAAACTATTTTTCTAGTCTGAAAAACCCAAAACTCAGGGAGGAACAGGAGGCC
GCTCGGCGACGACAGCAGCGCGAAAGCAAGAGCAACGCGGCCACGCCCACTAAGGGCCCA
GAGGGCAAAGTGGCCGGACCTGCCGACGCCCCCATGGACTCTGGTGCTGAGGAAGAGAAG
GCGGGAGCAGCCACCGTGAAGAAGCCGTCTCCCTCCAAAGCCCGCAAGAAGAAGCTAAAC
AAGAAGGGGAGGAAGATGGCTGGCCGCAAGCGCGGGCGGCCCAAGAAGATGAACACTGCG
AACCCCGAGCGGAAGCCCAAGAAGAACCAAACTGCACTGGATGCCCTGCACGCTCAGACC
GTGTCTCAGACGGCGGCCTCCTCACCCCAGGATGCCTACAGATCCCCTCACAGCCCGTTC
TACCAGCTACCTCCGAGCGTGCAGCGGCACTCCCCCAACCCGCTGCTGGTGGCGCCCACC
CCGCCCGCGCTGCAGAAGCTTCTAGAGTCCTTCAAGATCCAGTACCTCCAGTTCCTGGCA
TACACAAAGACCCCCCAGTACAAGGCCAGCCTGCAGGAGCTGCTTGGCCAGGAGAAGGAG
AAGAACGCCCAGCTGCTGGGTGCGGCTCAGCAGCTCCTCAGCCACTGCCAGGCCCAGAAG
GAGGAGATCAGGAGGCTGTTTCAGCAAAAATTGGATGAGCTTGGTGTGAAGGCGCTCACC
TACAACGACCTGATTCAAGCGCAGAAGGAGATCTCCGCCCATAACCAGCAGCTCCGGGAG
CAGTCCGAGCAGCTGGAGCAGGACAACCGCGCGCTCCGCGGCCAGAGCTTGCAGCTGCTC
AAGGCTCGCTGCGAGGAGCTGCAGCTGGACTGGGCCACGCTGTCGCTCGAGAAGCTGTTG
AAGGAGAAGCAGGCCCTGAAGAGCCAGATCTCGGAGAAGCAGAGGCACTGCCTGGAGCTG
CAGATCAGCATTGTGGAGCTAGAGAAGAGCCAGCGGCAGCAGGAGCTCCTGCAGCTCAAG
TCCTGTGTGCCGCCTGACGACGCCCTGTCCCTGCACCTTCGTGGGAAGGGCGCCCTGGGC
CGCGAGCTTGAGCCTGACGCCAGTCGGCTGCACCTGGAGCTGGACTGCACCAAGTTCTCG
CTGCCTCACTTGAGCAGCATGAGCCCGGAGCTCTCCATGAACGGCCAGGCTGCTGGCTAT
GAGCTCTGCGGTGTGCTGAGCCGGCCTTCGTCGAAGCAGAACACGCCCCAGTACCTGGCC
TCACCCCTGGACCAGGAGGTGGTGCCTTGTACACCTAGCCACGTCGGCCGGCCCCGCCTG
GAGAAGCTGTCCGGCCTGGCCGCACCCGACTACACTAGGCTGTCCCCGGCCAAGATTGTG
CTCAGGCGGCACCTGAGCCAGGACCACACAGTGCCCGGCCGGCCGGCTGCCAGTGAGCTC
CATTCGAGAGCCGAGCACACCAAGGAGAACGGCCTTCCCTACCAGAGCCCCAGCGTGCCT
GGCAGCATGAAGCTGAGCCCTCAGGACCCGCGGCCTCTGTCCCCTGGGGCCTTGCAGCTT
GCTGGAGAGAAGAGCAGCGAGAAGGGCCTGAGAGAGCGCGCCTACGGCAGCAGCGGGGAG
CTCATCACCAGCCTGCCCATCAGTATCCCGCTCAGCACCGTGCAGCCCAACAAGCTCCCG
GTCAGCATTCCCCTGGCCAGCGTCGTGCTGCCCAGTCGCGCCGAGAGGGCGAGGAGCACC
CCCAGTCCTGTGCTGCAGCCTCGTGACCCCTCGTCCACACTGGAAAAGCAGATTGGCGCT
AATGCCCACGGTGCTGGGAGCAGAAGCCTTGCCCTGGCCCCCGCAGGATTCTCCTATGCT
GGCTCTGTGGCCATCAGCGGGGCCCTGGCCGGCAGCCCGGCCTCTCTCACACCTGGTGCC
GAGCCGGCTACCTTGGATGAGTCCTCCAGTTCTGGGAGCCTTTTTGCCACCGTGGGGTCC
CGCAGCTCCACACCCCAGCACCCCCTGTTGCTGGCACAGCCCCGGAATTCGCTTCCTGCC
TCTCCCGCCCACCAGCTCTCCTCCAGCCCCCGGCTTGGTGGGGCCGCTCAGGGACCGTTG
CCCGAGGCTAGCAAGGGAGATCTGCCCTCCGATTCCGGCTTCTCAGATCCTGAGAGTGAA
GCTAAGAGGAGGATTGTGTTCACCATCACCACTGGTGCCGGCAGTGCCAAGCAGTCGCCC
TCCAGCAAGCACAGCCCCCTGACCGCCAGCGCCCGTGGGGACTGTGTTCCGAGTCACGGA
CAGGACAGTCGCAGGCGCGGCAGGCGGAAGCGAGCATCTGCTGGGACGCCCAGCTTAAGC
GCAGGCGTGTCCCCCAAGCGCAGAGCCCTCCCGTCCGTCGCTGGCCTTTTCACACAGCCT
TCGGGGTCCCCCCTCAACCTCAACTCCATGGTCAGTAACATCAACCAGCCCCTGGAGATT
ACAGCCATCTCGTCCCCGGAGACATCCCTGAAAAGCTCCCCTGTGCCCTACCAGGACCAC
GACCAGCCCCCCGTGCTTAAGAAGGAGCGGCCTCTGAGCCAGACCAATGGGGCACACTAC
TCCCCACTCACCTCAGACGAGGAGCCAGGCTCTGAGGACGAGCCCAGCAGTGCTCGAATT
GAGAGAAAAATTGCAACAATCTCCTTAGAAAGCAAATCTCCCCCGAAAACCTTGGAAAAT
GGTGGTGGCTTGGCGGGAAGGAAGCCCGCGCCCGCCGGCGAGCCAGTCAATAGCAGCAAG
TGGAAGTCCACCTTCTCGCCCATTTCCGACATCGGCCTGGCTAAGTCGGCGGACAGCCCG
CTGCAGGCCAGCTCCGCCCTCAGCCAGAACTCCCTGTTCACGTTCCGGCCCGCCCTGGAG
GAGCCCTCTGCCGATGCCAAGCTGGCCGCTCACCCTAGGAAGGGCTTTCCCGGCTCCCTG
TCGGGGGCTGATGGACTCAGCCCGGGCACCAACCCTGCCAACGGCTGCACCTTCGGCGGG
GGACTGGCCGCGGACCTGAGTTTACACAGCTTCAGTGATGGTGCTTCTCTTCCCCACAAG
GGCCCCGAGGCGGCCGGCCTGAGCTCCCCGCTGAGCTTCCCCTCGCAGCGCGGCAAGGAG
GGCTCGGACGCCAACCCTTTCCTGAGCAAGAGGCAGCTTGACGGCCTGGCTGGGCTGAAG
GGCGAGGGCAGCCGAGGCAAGGAGGCAGGGGAAGGCGGCCTGCCTCTGTGCGGGCCAACG
GACAAGACCCCACTGCTGAGCGGCAAGGCCGCCAAAGCCCGGGACCGCGAGGTGGACCTC
AAGAATGGCCACAACCTCTTCATCTCTGCGGCGGCCGTCCCTCCTGGAAGCCTCCTCAGC
GGCCCCGGCCTGGCCCCGGCTGCATCTTCCGCAGGCGGAGCGGCTTCCTCTGCTCAAACG
CATAGGTCTTTCCTGGGCCCCTTCCCGCCTGGCCCGCAGTTCGCGCTCGGCCCCATGTCC
CTGCAGGCTAACCTCGGATCCGTGGCCGGCTCTTCCGTGCTGCAGTCGCTCTTCAGCTCC
GTGCCCGCCGCCGCAGGCCTGGTGCACGTGTCCTCCGCTGCCACCAGGCTGACCAACTCG
CACGCCATGGGCAGTTTTTCCGGGGTGGCAGGCGGCACAGTTGGAGGTAAC
Protein sequence predicted based on nucleotide sequence
MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVL IDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVT DPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTDDDLFVDLGSGVGQVVLQVAAATNCK HHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVIFV NNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLK GSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRESKSNAATPTKGP EGKVAGPADAPMDSGAEEEKAGAATVKKPSPSKARKKKLNKKGRKMAGRKRGRPKKMNTA NPERKPKKNQTALDALHAQTVSQTAASSPQDAYRSPHSPFYQLPPSVQRHSPNPLLVAPT PPALQKLLESFKIQYLQFLAYTKTPQYKASLQELLGQEKEKNAQLLGAAQQLLSHCQAQK EEIRRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQSEQLEQDNRALRGQSLQLL KARCEELQLDWATLSLEKLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLK SCVPPDDALSLHLRGKGALGRELEPDASRLHLELDCTKFSLPHLSSMSPELSMNGQAAGY ELCGVLSRPSSKQNTPQYLASPLDQEVVPCTPSHVGRPRLEKLSGLAAPDYTRLSPAKIV LRRHLSQDHTVPGRPAASELHSRAEHTKENGLPYQSPSVPGSMKLSPQDPRPLSPGALQL AGEKSSEKGLRERAYGSSGELITSLPISIPLSTVQPNKLPVSIPLASVVLPSRAERARST PSPVLQPRDPSSTLEKQIGANAHGAGSRSLALAPAGFSYAGSVAISGALAGSPASLTPGA EPATLDESSSSGSLFATVGSRSSTPQHPLLLAQPRNSLPASPAHQLSSSPRLGGAAQGPL PEASKGDLPSDSGFSDPESEAKRRIVFTITTGAGSAKQSPSSKHSPLTASARGDCVPSHG QDSRRRGRRKRASAGTPSLSAGVSPKRRALPSVAGLFTQPSGSPLNLNSMVSNINQPLEI TAISSPETSLKSSPVPYQDHDQPPVLKKERPLSQTNGAHYSPLTSDEEPGSEDEPSSARI ERKIATISLESKSPPKTLENGGGLAGRKPAPAGEPVNSSKWKSTFSPISDIGLAKSADSP LQASSALSQNSLFTFRPALEEPSADAKLAAHPRKGFPGSLSGADGLSPGTNPANGCTFGG GLAADLSLHSFSDGASLPHKGPEAAGLSSPLSFPSQRGKEGSDANPFLSKRQLDGLAGLK GEGSRGKEAGEGGLPLCGPTDKTPLLSGKAAKARDREVDLKNGHNLFISAAAVPPGSLLS GPGLAPAASSAGGAASSAQTHRSFLGPFPPGPQFALGPMSLQANLGSVAGSSVLQSLFSS VPAAAGLVHVSSAATRLTNSHAMGSFSGVAGGTVGGN
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
