Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746500
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5682nts
Open reading frame: 1 to 5682
ATGAAAGCGATGCCCTGGAACTGGACCTGCCTTCTCTCCCACCTCCTCATGGTGGGCATG
GGCTCCTCCACTTTGCTCACCCGCCAGCCAGCCCCTCTGTCCCAGAAGCAGCGCTCATTT
GTCACATTCCGAGGAGAGCCTGCCGAGGGTTTCAATCACCTGGTGGTGGATGAGAGGACC
GGACACATTTACTTGGGGGCCGTCAATCGGATTTACAAGCTCTCCAGCGACCTGAAGGTC
TTGGTGACGCATGAGACAGGGCCGGACGAGGACAACCCCAAGTGTTACCCACCCCGCATC
GTCCAGACCTGCAATGAGCCCCTGACCACCACCAACAATGTCAACAAGATGCTCCTCATA
GACTACAAGGAGAACAGGCTGATTGCCTGTGGGAGCCTGTACCAAGGCATCTGCAAGCTG
CTGAGGCTGGAGGACCTCTTCAAGCTGGGGGAGCCTTATCATAAGAAGGAGCACTATCTG
TCAGGTGTCAACGAGAGCGGCTCAGTCTTTGGAGTGATCGTCTCCTACAGCAACCTGGAT
GACAAGCTGTTCATTGCCACGGCAGTGGATGGGAAGCCCGAGTATTTTCCCACCATCTCC
AGCAGGAAACTCACCAAGAACTCTGAGGCGGATGGCATGTTCGCGTACGTCTTCCATGAT
GAGTTCGTGGCCTCGATGATTAAGATCCCTTCGGACACCTTCACCATCATCCCTGACTTT
GATATCTACTATGTCTATGGTTTTAGCAGTGGCAACTTTGTCTACTTTTTGACCCTCCAA
CCTGAGATGGTGTCTCCACCAGGCTCCACCACCAAGGAACAGGTGTATACATCCAAGCTC
GTGAGGCTTTGCAAGGAGGACACAGCCTTCAACTCCTATGTAGAGGTGCCCATCGGCTGT
GAGCGCAGTGGGGTGGAGTACCGCCTGCTGCAGGCTGCCTACCTGTCCAAAGCGGGGGCC
GTGCTTGGCAGGACCCTTGGAGTCCATCCAGATGATGACCTGCTCTTCACCGTCTTCTCC
AAGGGCCAGAAGCGGAAAATGAAATCCCTGGATGAGTCGGCCCTGTGCATCTTCATCTTG
AAGCAGATAAATGACCGCATTAAGGAGCGGCTGCAGTCTTGTTACCGGGGCGAGGGCACG
CTGGACCTGGCCTGGCTCAAGGTCAAGGACATCCCCTGCAGCAGTGCGCTCTTAACCATT
GACGATAACTTCTGTGGCTTGGACATGAATGCTCCCCTAGGAGTGTCCGACATGGTGCGT
GGAATTCCCGTCTTCACGGAGGACAGGGACCGCATGACGTCGGTCATCGCCTATGTCTAC
AAGAACCACTCTCTGGCCTTTGTGGGCACCAAAAGCGGCAAGCTGAAGAAGATCCGGGTG
GATGGACCCAGAGGCAACGCCCTCCAGTATGAGACGGTGCAGGTTGTGGACCCCGGCCCA
GTCCTCCGGGATATGGCCTTCTCCAAGGACCACGAGCAACTCTACATCATGTCAGAGAGG
CAGCTGACCAGAGTCCCAGTGGAGTCCTGTGGTCAGTATCAGAGCTGCGGCGAGTGCCTT
GGCTCAGGCGACCCCCACTGTGGCTGGTGTGTTCTGCACAACACTTGCACCCGGAAGGAG
CGGTGTGAGCGGTCCAAGGAGCCCCGCAGGTTTGCCTCGGAGATGAAGCAGTGTGTCCGG
CTGACGGTCCATCCCAACAATATCTCCGTTTCTCAGTACAACGTGCTGCTGGTACTGGAG
ACGTACAATGTCCCGGAGCTGTCAGCTGGCGTCAACTGCACCTTTGAGGACCTGTCAGAG
ATGGATGGGCTGGTCGTGGGCAATCAGATCCAGTGCTACTCCCCTGCGGCCAAGGAGGTG
CCCCGCATCATCACAGAGAATGGCGACCACCATGTCGTACAGCTTCAGCTTAAATCAAAG
GAAACCGGCATGACCTTCGCCAGCACCAGCTTTGTCTTCTACAATTGCAGCGTCCACAAT
TCGTGCCTGTCCTGCGTGGAGAGTCCATACCGCTGCCACTGGTGTAAATACCGGCATGTC
TGCACCCATGACCCCAAGACCTGTTCCTTCCAGGAAGGTCGAGTGAAGCTACCCGAGGAC
TGCCCCCAGCTGCTCCGAGTGGACAAGATCCTAGTGCCCGTGGAGGTGATCAAGCCTATC
ACGCTGAAGGCCAAGAACCTCCCCCAGCCCCAGTCTGGGCAGCGTGGATACGAATGCATC
CTCAACATTCAGGGCAGCGAGCAGCGAGTTCCCGCCCTGCGCTTCAACAGCTCCAGCGTA
CAGTGCCAGAACACCTCTTATTCCTATGAAGGGATGGAGATCAACAACCTGCCCGTGGAG
TTGACAGTCGTGTGGAATGGGCACTTCAACATTGACAACCCAGCTCAGAATAAAGTTCAC
CTCTACAAGTGTGGAGCCATGCGTGAGAGCTGCGGGCTGTGCCTCAAGGCTGACCCAGAC
TTCGCATGTGGCTGGTGCCAGGGCCCAGGCCAGTGCACCCTGCGACAGCACTGCCCTGCC
CAGGAGAGCCAGTGGCTGGAGCTGTCTGGTGCCAAAAGCAAGTGCACAAACCCCCGCATC
ACAGAGATAATCCCGGTGACAGGCCCCCGAGAAGGGGGAACCAAGGTCACTATCCGAGGG
GAGAACCTGGGCCTGGAATTTCGCGACATCGCCTCGCATGTCAAGGTTGCTGGCGTGGAG
TGCAGCCCTTTAGTGGATGGTTACATCCCTGCAGAACAGATCGTGTGTGAGATGGGGGAG
GCCAAGCCCAGCCAGCATGCAGGCTTCGTGGAGATCTGCGTGGCTGTGTGTCGGCCTGAA
TTCATGGCCCGGTCCTCCCAGCTCTATTACTTCATGACACTGACACTCTCAGATCTGAAG
CCCAGCCGGGGGCCCATGTCCGGAGGGACCCAAGTGACCATCACAGGAACCAACCTGAAT
GCCGGAAGCAACGTGGTGGTGATGTTTGGAAAGCAGCCGTGTCTCTTCCACAGGCGATCT
CCATCCTACATTGTCTGCAACACCACATCCTCAGATGAGGTGCTAGAGATGAAGGTGTCG
GTGCAGGTTGACAGGGCCAAGATCCACCAGGACCTGGTCTTTCAGTATGTGGAAGACCCC
ACCATCGTGCGGATTGAGCCAGAATGGAGCATTGTCAGTGGAAACACACCCATCGCCGTA
TGGGGGACCCACCTTGACCTCATACAGAACCCCCAGATCCGTGCCAAGCATGGAGGGAAG
GAGCACATCAATATCTGTGAGGTTCTGAACGCTACTGAGATGACCTGTCAGGCGCCCGCC
CTCGCTCTGGGTCCTGACCACCAGTCAGACCTGACCGAGAGGCCCGAGGAGTTTGGCTTC
ATCCTGGACAACGTCCAGTCCCTGCTCATCCTCAACAAGACCAACTTCACCTACTATCCC
AACCCGGTGTTTGAGGCCTTTGGTCCCTCAGGAATCCTGGAGCTCAAGCCTGGCACGCCC
ATCATCCTAAAAGGCAAGAACCTGATCCCGCCTGTGGCTGGAGGCAACGTGAAGCTTAAC
TACACTGTGCTGGTAGGGGAGAAGCCGTGCACCGTGACCGTGTCAGATGTACAGCTGCTC
TGCGAGTCTCCCAACCTCATCGGCAGGCACAAAGTGATGGCCCGTGTCGGTGGCATGGAG
TACTCCCCGGGGATGGTGTACATTGCCCCTGACAGCCCGCTCAGCCTGCCCGCCATCGTG
AGCATCGCAGTGGCTGGCGGCCTCCTCATCATTTTCATCGTGGCCGTGCTCATTGCGTAT
AAACGCAAGTCCCGCGAAAGTGACCTCACGCTGAAGCGGTTGCAGATGCAGATGGACAAC
CTGGAGTCCCGTGTGGCCCTAGAATGCAAGGAAGCCTTTGCCGAGCTGCAGACGGACATC
CATGAGCTAACCTCTGACCTGGATGGAGCCGGAATTCCGTTCCTGGACTATAGAACTTAC
ACCATGCGGGTGCTGTTCCCAGGAATTGAAGACCACCCTGTGCTCCGGGACCTTGAGGTC
CCGGGCTACCGGCAGGAGCGTGTGGAGAAAGGCCTGAAGCTCTTCGCCCAGCTCATCAAC
AACAAGGTGTTCCTGCTGTCCTTCATCCGCACGCTTGAGTCCCAGCGTAGCTTCTCCATG
CGCGACCGTGGCAACGTGGCCTCACTCATCATGACCGTGCTGCAGAGCAAGCTGGAGTAC
GCCACTGATGTGCTGAAGCAGCTGCTCGCCGACCTCATTGACAAGAACCTGGAGTCCAAG
AACCACCCTAAGCTGCTGCTCAGGAGGACAGAGTCAGTGGCTGAGAAGATGCTGACCAAT
TGGTTTACTTTCCTGCTCTACAAGTTCCTCAAGGAGTGTGCTGGGGAGCCACTCTTCTCC
CTGTTCTGTGCCATCAAGCAGCAGATGGAGAAGGGCCCCATAGACGCCATCACGGGCGAG
GCCCGCTACTCCTTGAGCGAGGACAAGCTCATCCGCCAGCAGATTGACTACAAAACCCTG
GTCCTGAGCTGTGTCAGCCCAGACAATGCCAACAGCCCCGAGGTCCCAGTAAAGATCCTC
AACTGTGACACCATCACTCAGGTAAAGGAGAAGATTCTGGATGCCATCTTCAAGAATGTG
CCTTGCTCCCACCGGCCCAAAGCTGCAGATATGGATCTGGAGTGGCGGCAAGGCAGTGGG
GCAAGGATGATCTTGCAGGATGAAGACATCACCACCAAGATTGAGAATGATTGGAAGCGA
CTGAACACACTGGCCCACTACCAGGTGCCAGATGGTTCCGTGGTGGCATTAGTGTCCAAG
CAGGTCACAGCCTATAACGCAGTGAACAACTCCACCGTCTCCAGGACCTCAGCAAGTAAA
TATGAAAACATGATCAGGTACACGGGCAGCCCCGACAGCCTCCGCTCACGGACACCTATG
ATCACTCCTGACCTGGAGAGTGGAGTCAAGATGTGGCACCTAGTGAAGAACCACGAGCAC
GGGGACCAGAAGGAGGGGGACCGGGGGAGCAAGATGGTGTCTGAAATCTACCTGACCAGA
CTCCTGGCCACTAAGGGCACACTGCAGAAGTTTGTGGATGACCTATTTGAGACTATCTTC
AGCACGGCACACCGTGGCTCTGCCCTGCCCCTGGCCATCAAGTACATGTTTGACTTCCTG
GATGAGCAGGCTGATAAACATGGCATTCATGACCCGCACGTGCGCCATACCTGGAAGAGC
AATTGCCTGCCCCTCAGGTTTTGGGTCAACATGATCAAGAACCCGCAGTTTGTGTTTGAC
ATCCATAAGAACAGCATCACAGACGCCTGCCTCTCTGTGGTGGCTCAGACCTTCATGGAC
TCTTGCTCCACGTCAGAGCACCGGCTGGGCAAGGACTCGCCCTCCAACAAGCTGCTGTAT
GCCAAGGACATCCCCAGCTACAAGAATTGGGTGGAGAGGTATTACTCAGACATAGGGAAG
ATGCCAGCTATCAGCGACCAAGACATGAACGCATACCTGGCTGAGCAGTCCCGGATGCAC
ATGAATGAGTTCAACACCATGAGTGCACTATCGGAGATCTTCTCCTATGTGGGCAAATAC
AGCGAGGAGATCCTTGGACCTCTGGACCACGATGACCAGTGTGGGAAGCAGAAACTGGCC
TACAAACTAGAACAAGTCATAACCCTAATGAGTTTAGACAGC
Protein sequence predicted based on nucleotide sequence
MKAMPWNWTCLLSHLLMVGMGSSTLLTRQPAPLSQKQRSFVTFRGEPAEGFNHLVVDERT GHIYLGAVNRIYKLSSDLKVLVTHETGPDEDNPKCYPPRIVQTCNEPLTTTNNVNKMLLI DYKENRLIACGSLYQGICKLLRLEDLFKLGEPYHKKEHYLSGVNESGSVFGVIVSYSNLD DKLFIATAVDGKPEYFPTISSRKLTKNSEADGMFAYVFHDEFVASMIKIPSDTFTIIPDF DIYYVYGFSSGNFVYFLTLQPEMVSPPGSTTKEQVYTSKLVRLCKEDTAFNSYVEVPIGC ERSGVEYRLLQAAYLSKAGAVLGRTLGVHPDDDLLFTVFSKGQKRKMKSLDESALCIFIL KQINDRIKERLQSCYRGEGTLDLAWLKVKDIPCSSALLTIDDNFCGLDMNAPLGVSDMVR GIPVFTEDRDRMTSVIAYVYKNHSLAFVGTKSGKLKKIRVDGPRGNALQYETVQVVDPGP VLRDMAFSKDHEQLYIMSERQLTRVPVESCGQYQSCGECLGSGDPHCGWCVLHNTCTRKE RCERSKEPRRFASEMKQCVRLTVHPNNISVSQYNVLLVLETYNVPELSAGVNCTFEDLSE MDGLVVGNQIQCYSPAAKEVPRIITENGDHHVVQLQLKSKETGMTFASTSFVFYNCSVHN SCLSCVESPYRCHWCKYRHVCTHDPKTCSFQEGRVKLPEDCPQLLRVDKILVPVEVIKPI TLKAKNLPQPQSGQRGYECILNIQGSEQRVPALRFNSSSVQCQNTSYSYEGMEINNLPVE LTVVWNGHFNIDNPAQNKVHLYKCGAMRESCGLCLKADPDFACGWCQGPGQCTLRQHCPA QESQWLELSGAKSKCTNPRITEIIPVTGPREGGTKVTIRGENLGLEFRDIASHVKVAGVE CSPLVDGYIPAEQIVCEMGEAKPSQHAGFVEICVAVCRPEFMARSSQLYYFMTLTLSDLK PSRGPMSGGTQVTITGTNLNAGSNVVVMFGKQPCLFHRRSPSYIVCNTTSSDEVLEMKVS VQVDRAKIHQDLVFQYVEDPTIVRIEPEWSIVSGNTPIAVWGTHLDLIQNPQIRAKHGGK EHINICEVLNATEMTCQAPALALGPDHQSDLTERPEEFGFILDNVQSLLILNKTNFTYYP NPVFEAFGPSGILELKPGTPIILKGKNLIPPVAGGNVKLNYTVLVGEKPCTVTVSDVQLL CESPNLIGRHKVMARVGGMEYSPGMVYIAPDSPLSLPAIVSIAVAGGLLIIFIVAVLIAY KRKSRESDLTLKRLQMQMDNLESRVALECKEAFAELQTDIHELTSDLDGAGIPFLDYRTY TMRVLFPGIEDHPVLRDLEVPGYRQERVEKGLKLFAQLINNKVFLLSFIRTLESQRSFSM RDRGNVASLIMTVLQSKLEYATDVLKQLLADLIDKNLESKNHPKLLLRRTESVAEKMLTN WFTFLLYKFLKECAGEPLFSLFCAIKQQMEKGPIDAITGEARYSLSEDKLIRQQIDYKTL VLSCVSPDNANSPEVPVKILNCDTITQVKEKILDAIFKNVPCSHRPKAADMDLEWRQGSG ARMILQDEDITTKIENDWKRLNTLAHYQVPDGSVVALVSKQVTAYNAVNNSTVSRTSASK YENMIRYTGSPDSLRSRTPMITPDLESGVKMWHLVKNHEHGDQKEGDRGSKMVSEIYLTR LLATKGTLQKFVDDLFETIFSTAHRGSALPLAIKYMFDFLDEQADKHGIHDPHVRHTWKS NCLPLRFWVNMIKNPQFVFDIHKNSITDACLSVVAQTFMDSCSTSEHRLGKDSPSNKLLY AKDIPSYKNWVERYYSDIGKMPAISDQDMNAYLAEQSRMHMNEFNTMSALSEIFSYVGKY SEEILGPLDHDDQCGKQKLAYKLEQVITLMSLDS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
