Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
Codon optimized
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR221
Format:
FUSION
Source:
None
Description:
None
Comments:
Gene synthesis by Gen9. Codon optimized
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
Center for Personalized Diagnostics
HsCD00746508
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5814nts
Open reading frame: 1 to 5814
ATGAGCTCTGACGCAGAAATGGCCATTTTTGGAGAAGCAGCTCCCTACCTCCGGAAACCA
GAGAAGGAGAGAATCGAGGCTCAAAATCGTCCATTCGATTCCAAGAAAGCCTGCTTTGTA
GCGGATAATAAGGAAATGTATGTGAAAGGCATGATCCAGACCAGGGAAAATGACAAAGTC
ATAGTCAAGACCCTCGATGACCGGATGCTGACTCTGAACAATGACCAGGTCTTCCCCATG
AACCCTCCCAAATTTGACAAGATCGAGGACATGGCTATGATGACTCACCTCCATGAACCT
GCTGTTCTGTACAACCTCAAAGAGCGCTATGCAGCCTGGATGATCTACACCTACTCAGGC
CTCTTCTGTGTCACCGTCAACCCCTACAAGTGGCTGCCGGTGTACAAGCCCGAGGTGGTG
GCTGCCTACAGAGGCAAAAAGCGGCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGAC
AATGCCTATCAGTTCATGCTGACTGATCGAGACAACCAGTCTATCCTCATCACCGGAGAA
TCCGGGGCCGGGAAGACTGTGAACACCAAGCGTGTCATCCAGTATTTTGCTACAATTGCA
GTTACCGGGGACAAGAAGAAGGAGACACAGCCAGGCAAAATGCAGGGAACCCTAGAGGAT
CAGATCATACAGGCCAACCCACTCCTCGAGGCCTTTGGAAATGCCAAGACTGTTAGGAAT
GATAACTCCTCGAGATTTGGGAAGTTCATTCGGATTCATTTTGGAGCCACAGGAAAGCTG
GCATCGGCAGACATCGAAACTTATCTGTTAGAAAAATCCAGAGTGACGTTTCAATTATCC
AGTGAGAGAAGCTATCATATTTTCTACCAAATTATGTCAAACAAGAAGCCAGAACTAATT
GACCTGCTTCTGATCTCCACCAACCCCTTCGACTTCCCCTTCGTGAGCCAAGGAGAGGTC
ACGGTAGCCAGTATCGATGACAGTGAAGAACTGCTGGCGACAGATAATGCCATTGACATC
CTGGGCTTCAGCTCCGAGGAGAAAGTCGGGATCTACAAACTGACGGGAGCCGTCATGCAT
TATGGGAACATGAAGTTCAAGCAGAAGCAGCGTGAGGAGCAGGCGGAGCCAGACGGCACC
GAAGTGGCTGACAAAGCTGGATACCTGATGGGCCTGAATTCTGCTGAAATGCTGAAGGGC
CTGTGCTGTCCAAGGGTGAAGGTTGGCAATGAATATGTCACTAAAGGGCAAAATGTCCAA
CAGGTGACCAATTCGGTGGGTGCTCTGGCCAAAGCCGTGTACGAGAAGATGTTCCTGTGG
ATGGTCACCCGGATTAACCAGCAGCTGGACACCAAGCAGCCCAGACAGTACTTCATCGGG
GTCTTGGACATTGCTGGCTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATC
AACTTCACCAATGAGAAACTGCAACAGTTTTTCAACCACCACATGTTCGTGCTGGAGCAG
GAAGAGTACAAGAAGGAAGGCATCGAGTGGGAGTTCATTGACTTCGGAATGGACCTTGCT
GCCTGCATCGAGCTTATCGAGAAGCCTATGGGCATTTTCTCCATCCTGGAAGAGGAGTGT
ATGTTCCCCAAGGCAACAGACACCTCATTCAAGAACAAGCTGTATGACCAGCATCTTGGA
AAATCCAACAACTTCCAGAAGCCCAAGCCTGCCAAAGGCAAGGCTGAGGCTCACTTCTCG
CTGGTGCACTATGCCGGCACTGTCGACTACAACATCGCTGGCTGGCTGGACAAAAACAAG
GACCCCCTGAACGAGACTGTGGTGGGGCTGTACCAGAAGTCTTCCCTGAAGCTTCTCTCC
TTCCTTTTTTCCAACTATGCTGGTGCAGAGACAGGCGACTCTGGAGGCAGCAAGAAGGGC
GGCAAGAAGAAGGGATCCTCTTTCCAGACCGTGTCGGCCGTGTTCAGGGAAAATCTAAAC
AAATTGATGACTAACTTAAGAAGCACCCACCCTCACTTTGTACGATGTCTGATTCCCAAT
GAGACTAAGACTCCTGGTGTGATGGACCACTACCTGGTCATGCACCAGCTGCGCTGTAAC
GGGGTCCTCGAAGGCATCCGGATTTGCAGGAAGGGATTCCCCAGTCGGATCCTCTATGCT
GACTTCAAGCAGCGGTACCGGATCCTCAATGCCAGTGCTATCCCTGAAGGGCAGTTCATT
GACAGCAAAAATGCCTCAGAGAAGCTTCTCAACTCCATCGATGTGGACCGGGAGCAGTTC
AGGTTCGGCAACACCAAGGTGTTTTTCAAAGCTGGGCTCCTGGGACTTCTGGAGGAGATG
AGAGATGAGAAGCTGGTGACGCTGATGACAAGCACGCAAGCGGTGTGCAGGGGATACCTG
ATGAGGGTGGAGTTCAAGAAGATGATGGAGAGGAGGGACTCCATCTTCTGCATCCAGTAC
AACATCCGCTCTTTTATGAACGTCAAGCACTGGCCCTGGATGAACCTGTTCTTCAAAATC
AAGCCCCTGCTGAAGAGTGCAGAGGCCGAGAAGGAGATGGCCACCATGAAGGAAGACTTT
GAGAGGACCAAGGAAGAACTGGCCCGATCTGAGGCTCGCCGGAAGGAGCTTGAGGAGAAA
ATGGTGTCCCTCCTGCAGGAGAAGAATGACCTGCAATTGCAGGTCCAGTCTGAAACAGAA
AATCTGATGGACGCTGAGGAACGGTGTGAAGGACTTATCAAAAGCAAGATCCTACTCGAA
GCAAAAGTCAAGGAGCTGACGGAGAGATTGGAAGAGGAAGAGGAGATGAATTCTGAATTG
GTGGCCAAGAAGAGAAATCTGGAAGATAAATGCTCCTCTCTCAAGAGAGACATTGATGAC
CTGGAGCTGACCTTGACGAAAGTTGAAAAGGAGAAGCATGCTACAGAGAACAAGGTCAAG
AATCTGTCCGAAGAAATGACAGCTCTTGAAGAAAACATCTCCAAATTGACCAAAGAAAAA
AAATCTCTACAGGAGGCCCATCAGCAAACCCTGGATGATCTTCAGGTCGAAGAAGATAAA
GTCAATGGTCTAATCAAAATAAATGCCAAGCTTGAACAGCAAACAGATGATCTTGAGGGT
TCCTTAGAGCAGGAGAAGAAACTGCGGGCGGACTTGGAAAGGGCGAAGAGGAAACTGGAA
GGAGATCTGAAAATGTCCCAGGAATCTATTATGGATCTAGAAAATGACAAGCAGCAAATA
GAAGAGAAATTGAAAAAGAAGGAGTTTGAACTCAGTCAGTTACAAGCTAAAATAGATGAC
GAACAAGTCCACAGTTTGCAGTTTCAAAAGAAGATCAAAGAACTGCAAGCCCGCATAGAA
GAGCTGGAGGAGGAAATTGAAGCGGAACATACGCTCAGGGCCAAGATTGAGAAGCAGCGC
TCAGATTTGGCCAGAGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCAGTGGGGCC
ACTTCAGCCCAGATTGAGATGAACAAGAAGAGGGAGGCCGAGTTCCAGAAAATGCGCAGG
GACCTGGAGGAGGCCACATTGCAGCACGAAGCCACAGCAGCCACCCTGAGGAAGAAGCAA
GCAGACAGTGTGGCTGAGCTTGGGGAGCAGATTGACAACCTGCAGCGGGTGAAGCAGAAG
CTGGAGAAGGAGAAGAGCGAGCTCAAGATGGAGATTGACGACATGGCCAGCAACATCGAG
GCTCTCTCCAAGTCAAAGAGTAACATAGAAAGAACGTGCCGGACGGTAGAAGATCAATTT
AGTGAAATCAAAGCCAAGGACGAGCAACAGACACAGTTGATCCATGATCTGAACATGCAG
AAAGCAAGACTGCAGACCCAAAATGGGGAGCTTTCTCACCGAGTGGAAGAGAAGGAGTCC
CTGATTTCACAGCTCACAAAAAGCAAGCAGGCCCTCACCCAGCAGCTGGAGGAGCTTAAG
AGGCAAATGGAAGAAGAAACCAAGGCCAAGAACGCCATGGCGCACGCCCTGCAGTCCTCC
CGGCACGACTGTGACCTGCTGCGGGAACAGTATGAGGAGGAGCAGGAAGCCAAGGCCGAG
CTCCAGAGGGCGCTGTCCAAGGCCAACAGTGAGGTTGCCCAGTGGAGGACCAAATACGAG
ACGGACGCCATTCAGCGCACAGAGGAGCTGGAGGAGGCTAAGAAAAAACTGGCCCAGAGG
CTCCAAGAAGCAGAGGAGAACACGGAGACGGCGAACTCTAAGTGCGCATCGTTGGAGAAA
ACCAAGCAGAGGCTGCAGGGAGAGGTGGAGGATCTGATGCGGGATCTGGAGCGCTCCCAC
ACCGCCTGTGCCACACTGGACAAGAAGCAGAGGAACTTCGACAAGGTCCTTGCAGAGTGG
AAGCAAAAGCTGGACGAAAGTCAGGCTGAGTTGGAAGCCGCTCAGAAGGAGTCCAGATCA
TTGAGCACCGAACTCTTCAAGATGAGGAATGCCTATGAGGAGGTCGTGGACCAGTTGGAA
ACACTGAGGCGAGAGAACAAAAATCTGCAAGAAGAGATTTCCGACTTAACCGAGCAGATT
GCAGAAACTGGCAAGAATCTGCAGGAAGCGGAAAAGACCAAAAAGCTAGTGGAGCAGGAA
AAGTCAGATCTCCAGGTAGCTTTGGAAGAAGTGGAGGGTTCCTTGGAACACGAGGAGAGC
AAGATCTTGCGCGTGCAGCTAGAGCTGAGCCAGGTGAAATCCGAGCTAGACCGCAAGGTG
ATTGAGAAGGATGAAGAAATCGAGCAGCTAAAAAGGAACAGCCAGCGGGCAGCAGAGGCC
CTCCAGAGCGTGCTGGATGCTGAAATCCGAAGCCGGAACGACGCTCTGAGGCTAAAGAAG
AAGATGGAGGGAGACTTAAACGAGATGGAGATTCAGCTGGGCCACTCCAACCGCCAGATG
GCCGAAACCCAGAAGCATCTGCGCACGGTCCAGGGCCAGCTCAAGGACTCTCAGCTGCAT
CTCGATGACGCCCTGCGGAGCAATGAGGACCTCAAGGAGCAGCTGGCTATCGTGGAGCGC
AGGAATGGCCTCCTGCTGGAGGAGCTGGAGGAAATGAAGGTGGCCCTGGAACAGACGGAG
CGGACCCGCAGACTGTCAGAGCAGGAGCTGCTGGACGCCAGCGACCGCGTCCAGCTCCTG
CACTCCCAGAACACAAGCCTGATAAATACAAAGAAAAAACTGGAGGCTGACATAGCTCAG
TGCCAGGCAGAGGTGGAGAACTCGATCCAGGAGTCCAGGAACGCAGAGGAGAAGGCCAAG
AAGGCCATCACGGATGCAGCTATGATGGCTGAGGAGCTAAAGAAGGAACAGGACACCAGC
GCCCACCTGGAGCGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCTCCAGCACCGT
CTAGATGAGGCTGAACAACTAGCGCTGAAGGGCGGGAAGAAGCAGATCCAGAAACTGGAG
AACCGGGTGCGGGAGCTGGAAAATGAGCTTGATGTGGAACAGAAGAGGGGAGCTGAAGCC
CTGAAGGGAGCCCACAAGTACGAACGAAAAGTCAAGGAGATGACATACCAAGCTGAGGAG
GACCACAAGAATATCCTTAGGCTCCAGGATCTGGTGGACAAGCTGCAGGCCAAAGTTAAG
TCTTACAAGAGGCAGGCTGAGGAGGCGGAGGAGCAGGCCAACACGCAGCTGTCCAGATGC
CGCAGAGTGCAGCATGAGCTAGAGGAGGCCGCGGAGAGGGCGGACATCGCTGAGTCGCAG
GTCAACAAACTGAGGGCCAAGAGCAGAGACGTGGGCAGCCAGAAGATGGAAGAA
Protein sequence predicted based on nucleotide sequence
MSSDAEMAIFGEAAPYLRKPEKERIEAQNRPFDSKKACFVADNKEMYVKGMIQTRENDKV IVKTLDDRMLTLNNDQVFPMNPPKFDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSG LFCVTVNPYKWLPVYKPEVVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRDNQSILITGE SGAGKTVNTKRVIQYFATIAVTGDKKKETQPGKMQGTLEDQIIQANPLLEAFGNAKTVRN DNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLSSERSYHIFYQIMSNKKPELI DLLLISTNPFDFPFVSQGEVTVASIDDSEELLATDNAIDILGFSSEEKVGIYKLTGAVMH YGNMKFKQKQREEQAEPDGTEVADKAGYLMGLNSAEMLKGLCCPRVKVGNEYVTKGQNVQ QVTNSVGALAKAVYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCI NFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEEC MFPKATDTSFKNKLYDQHLGKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNIAGWLDKNK DPLNETVVGLYQKSSLKLLSFLFSNYAGAETGDSGGSKKGGKKKGSSFQTVSAVFRENLN KLMTNLRSTHPHFVRCLIPNETKTPGVMDHYLVMHQLRCNGVLEGIRICRKGFPSRILYA DFKQRYRILNASAIPEGQFIDSKNASEKLLNSIDVDREQFRFGNTKVFFKAGLLGLLEEM RDEKLVTLMTSTQAVCRGYLMRVEFKKMMERRDSIFCIQYNIRSFMNVKHWPWMNLFFKI KPLLKSAEAEKEMATMKEDFERTKEELARSEARRKELEEKMVSLLQEKNDLQLQVQSETE NLMDAEERCEGLIKSKILLEAKVKELTERLEEEEEMNSELVAKKRNLEDKCSSLKRDIDD LELTLTKVEKEKHATENKVKNLSEEMTALEENISKLTKEKKSLQEAHQQTLDDLQVEEDK VNGLIKINAKLEQQTDDLEGSLEQEKKLRADLERAKRKLEGDLKMSQESIMDLENDKQQI EEKLKKKEFELSQLQAKIDDEQVHSLQFQKKIKELQARIEELEEEIEAEHTLRAKIEKQR SDLARELEEISERLEEASGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKQ ADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDMASNIEALSKSKSNIERTCRTVEDQF SEIKAKDEQQTQLIHDLNMQKARLQTQNGELSHRVEEKESLISQLTKSKQALTQQLEELK RQMEEETKAKNAMAHALQSSRHDCDLLREQYEEEQEAKAELQRALSKANSEVAQWRTKYE TDAIQRTEELEEAKKKLAQRLQEAEENTETANSKCASLEKTKQRLQGEVEDLMRDLERSH TACATLDKKQRNFDKVLAEWKQKLDESQAELEAAQKESRSLSTELFKMRNAYEEVVDQLE TLRRENKNLQEEISDLTEQIAETGKNLQEAEKTKKLVEQEKSDLQVALEEVEGSLEHEES KILRVQLELSQVKSELDRKVIEKDEEIEQLKRNSQRAAEALQSVLDAEIRSRNDALRLKK KMEGDLNEMEIQLGHSNRQMAETQKHLRTVQGQLKDSQLHLDDALRSNEDLKEQLAIVER RNGLLLEELEEMKVALEQTERTRRLSEQELLDASDRVQLLHSQNTSLINTKKKLEADIAQ CQAEVENSIQESRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHR LDEAEQLALKGGKKQIQKLENRVRELENELDVEQKRGAEALKGAHKYERKVKEMTYQAEE DHKNILRLQDLVDKLQAKVKSYKRQAEEAEEQANTQLSRCRRVQHELEEAAERADIAESQ VNKLRAKSRDVGSQKMEE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR221
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4762
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
CmR
confers resistance to chloramphenicol
1847
2506
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
3156
3965
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3027
3043
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2507
2609
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3004
3022
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2754
2985
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4112
4700
