Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
pLM70
Keyword:
"Spt16 1- 1035, Y972A, Spt16p; Construct Abbreviation: Spt16-Y972A"
Species:
Saccharomyces cerevisiae S288c
Type:
cDNA
Vector Name:
Yep_URA3
Format:
CLOSED
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Utah
David J. Kemble
David J. Kemble
ScCD00751521
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 3108nts
Open reading frame: 1 to 3108
ATGGAAGAGCTGAATATCGATTTTGACGTATTTAAGAAAAGGATTGAATTGTTGTATTCC
AAGTACAATGAGTTTGAAGGTTCTCCAAATTCGCTGCTGTTTGTTTTAGGTTCGTCCAAC
GCTGAAAACCCGTACCAGAAGACGACCATATTGCATAATTGGTTGCTAAGTTACGAATTT
CCCGCTACTTTGATTGCATTAGTTCCCGGAAAGGTTATTATAATTACTAGTTCTGCCAAG
GCCAAGCATTTACAAAAGGCAATTGATCTATTTAAAGACCCTGAAAGCAAAATTACGCTG
GAATTGTGGCAAAGAAACAATAAAGAACCAGAACTCAATAAAAAGTTATTTGATGACGTT
ATTGCCTTAATCAATAGCGCTGGTAAAACAGTGGGTATCCCTGAAAAGGACTCTTACCAA
GGTAAATTTATGACTGAGTGGAACCCAGTATGGGAAGCGGCTGTGAAGGAGAACGAATTC
AATGTCATTGATATTTCGCTGGGTCTTTCTAAAGTTTGGGAAGTGAAGGATGTTAACGAA
CAAGCCTTCCTGTCCGTTTCAAGTAAGGGGTCGGACAAATTTATGGACCTTTTATCCAAT
GAAATGGTTCGGGCAGTTGACGAGGAGTTGAAAATCACCAATGCCAAGTTATCAGACAAA
ATTGAAAATAAAATTGATGATGTGAAATTTTTGAAGCAATTGAGTCCCGATTTAAGCGCA
TTATGCCCACCAAACTACAAATTCAACTTTGATTTATTGGATTGGACCTATTCTCCAATC
ATTCAGTCCGGGAAAAAGTTTGATCTTAGGGTTTCTGCCCGTTCTACCAATGATCAGTTG
TACGGCAACGGTTGTATTTTAGCTTCATGTGGTATTCGTTACAATAATTATTGTTCTAAT
ATTACTAGGACCTTTTTGATCGATCCATCTGAAGAAATGGCCAACAACTACGATTTTCTA
TTAACTTTGCAAAAAGAAATTGTAACTAACATTCTGAAGCCCGGCCGTACACCAAAAGAA
GTTTATGAATCAGTAATAGAATACATCGAAAAAACCAAACCTGAACTAGTGCCCAATTTC
ACCAAAAACATTGGTTCATTGATTGGTCTCGAATTTAGAGATTCTAACTTTATATTAAAT
GTTAAGAATGATTACCGTAAAATCCAACGCGGTGACTGCTTTAATATTTCGTTTGGTTTC
AATAATCTGAAAGATTCTCAAAGTGCTAACAACTACGCTTTACAATTAGCTGATACGGTT
CAAATTCCTCTCGATGAAACAGAGCCTCCACGCTTCTTAACAAATTACACCAAAGCCAAA
TCCCAGATATCATTTTATTTCAATAATGAAGAAGAAGACAATAATAAAAAGAAATCTTCA
CCTGCCACCAAAGTCCCTAGTAAACCAGACAGAAATTCCAAAATTTTAAGAACAAAGTTA
CGTGGCGAAGCCCGTGGTGGTGCCGAAGACGCTCAAAAGGAGCAAATTCGTAAAGAAAAC
CAAAAGAAGCTACACGAAAAATTGGAAAAGAATGGATTACTCAGATTTAGTGCTGCTGAT
GCCAATGGCCCAGATAGTGAACCTCGTCAATACTTCAAAAAATATGAATCTTATGTTCGG
GATTCACAACTACCAACCAATATTCGTGATCTAAGAATTCATGTCGACTGGAAAAGCCAA
ACAATTATTCTACCCATTTACGGTAGGCCAGTCCCATTTCATATAAATTCATATAAGAAT
GGTTCTAAAAACGAAGAAGGCGAATATACGTATTTACGTTTGAACTTTAATTCACCGGGA
TCTTCTGGTGGTATTTCTAAAAAAGTGGAGGAATTGCCGTATGAGGAATCAGCAGACAAT
CAATTTGTACGTTCGATTACACTAAGATCCAAAGATGGTGACCGCATGAGTGAAACCTTT
AAACAAATTGCAGATTTGAAAAAAGAAGCCACAAAGAGAGAGCAAGAACGTAAGGCGCTT
GCTGATGTTGTTCAACAGGACAAATTGATTGAAAATAAAACTGGAAGAACGAAAAGACTG
GATCAAATTTTTGTGAGGCCAAATCCAGATACAAAACGTGTACCTAGTACTGTCTTTATC
CATGAAAATGGTATCAGATTTCAATCCCCACTAAGGACTGATAGCAGAATAGACATATTA
TTCTCAAACATTAAGAATCTAATTTTTCAATCTTGTAAAGGTGAATTAATTGTCGTCATT
CATATTCATTTGAAGAATCCAATTTTAATGGGTAAAAAGAAAATACAAGACGTCCAATTC
TATCGTGAAGCTTCTGATATGTCCGTCGATGAAACTGGAGGTGGAAGACGAGGTCAATCC
AGATTCAGAAGATATGGTGATGAGGATGAGCTGGAACAAGAACAAGAAGAAAGAAGAAAA
CGAGCTGCGCTTGATAAAGAATTTAAGTATTTTGCAGACGCAATCGCAGAAGCATCAAAC
GGTTTATTGACGGTGGAGAATACATTTAGAGATTTGGGCTTCCAAGGTGTCCCAAATAGA
TCGGCAGTTTTCTGTATGCCAACTACAGATTGTTTAGTTCAATTGATTGAACCACCATTT
TTGGTGATTAACCTAGAGGAAGTCGAAATCTGTATTTTAGAAAGAGTTCAATTTGGTTTG
AAAAACTTCGACATGGTTTTTGTTTATAAAGATTTCAATAAACCCGTCACTCATATCAAT
ACAGTTCCGATCGAATCTTTAGATTTCTTGAAGCAGTGGTTAACAGATATGGATATTCCT
TACACTGTCTCAACAATCAATTTGAATTGGGCAACTATTATGAAGTCATTACAAGATGAT
CCATATCAGTTTTTCTTAGATGGTGGTTGGAATTTTCTGGCTACTGGTTCAGATGATGAA
GCATCTGATGAAAGTGAAGAAGAAGTTAGTGAAGCAGAGGCTTCAGAAGACGACGTAAGT
GATGAAAGCGCATTTTCTGAAGATGAAGAAGGATCAGAGGTGGATGACGATATTAGTGGT
GATGAAAGTGAGGACTATACTGGCGACGAGAGTGAGGAAGGTGAAGACTGGGATGAATTA
GAGAAAAAGGCTGCTAGGGCTGATAGGGGTGCAAACTTTAGAGATTAG
Protein sequence predicted based on nucleotide sequence
MEELNIDFDVFKKRIELLYSKYNEFEGSPNSLLFVLGSSNAENPYQKTTILHNWLLSYEF PATLIALVPGKVIIITSSAKAKHLQKAIDLFKDPESKITLELWQRNNKEPELNKKLFDDV IALINSAGKTVGIPEKDSYQGKFMTEWNPVWEAAVKENEFNVIDISLGLSKVWEVKDVNE QAFLSVSSKGSDKFMDLLSNEMVRAVDEELKITNAKLSDKIENKIDDVKFLKQLSPDLSA LCPPNYKFNFDLLDWTYSPIIQSGKKFDLRVSARSTNDQLYGNGCILASCGIRYNNYCSN ITRTFLIDPSEEMANNYDFLLTLQKEIVTNILKPGRTPKEVYESVIEYIEKTKPELVPNF TKNIGSLIGLEFRDSNFILNVKNDYRKIQRGDCFNISFGFNNLKDSQSANNYALQLADTV QIPLDETEPPRFLTNYTKAKSQISFYFNNEEEDNNKKKSSPATKVPSKPDRNSKILRTKL RGEARGGAEDAQKEQIRKENQKKLHEKLEKNGLLRFSAADANGPDSEPRQYFKKYESYVR DSQLPTNIRDLRIHVDWKSQTIILPIYGRPVPFHINSYKNGSKNEEGEYTYLRLNFNSPG SSGGISKKVEELPYEESADNQFVRSITLRSKDGDRMSETFKQIADLKKEATKREQERKAL ADVVQQDKLIENKTGRTKRLDQIFVRPNPDTKRVPSTVFIHENGIRFQSPLRTDSRIDIL FSNIKNLIFQSCKGELIVVIHIHLKNPILMGKKKIQDVQFYREASDMSVDETGGGRRGQS RFRRYGDEDELEQEQEERRKRAALDKEFKYFADAIAEASNGLLTVENTFRDLGFQGVPNR SAVFCMPTTDCLVQLIEPPFLVINLEEVEICILERVQFGLKNFDMVFVYKDFNKPVTHIN TVPIESLDFLKQWLTDMDIPYTVSTINLNWATIMKSLQDDPYQFFLDGGWNFLATGSDDE ASDESEEEVSEAEASEDDVSDESAFSEDEEGSEVDDDISGDESEDYTGDESEEGEDWDEL EKKAARADRGANFRD*
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Mutations:
Y972A
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
ProteinExpressed: Protein_expressed
Recommended expression in:
Not Applicable
Vector Name:
Yep_URA3
Synonyms:
''
Description:
Vector Description
Comments:
None
Size (bp):
9802
Parent Vector:
None
Properties:
''
Author Name:
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
AmpR
5792
6652
CDS
Ura3
7089
7892
Gene
Spt16
713
3801
primer
M13 forward
9786
9802
primer
M13 reverse
4631
4647
promoter
Ura3 promoter
6873
7088
promoter
GAL1, 10
14
678
promoter
lac promoter
4679
4709
protein binding site
UAS
230
347
protein binding site
lac operator
4655
4671
protein binding site
CAP binding site
4724
4745
rep_origin
ori
5033
5621
rep_origin
2 micron ori
8003
9349
