Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
WISP08-94 pCAG CHMP7-myc
Keyword:
"Full length CHMP7 protein with C-terminal myc tag, Sundquist lab internal ID:WISP08-94"
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pCAG.MCS2.C-Myc
Format:
FUSION
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
Uta von Schwedler
Wesley I. Sundquist
Uta von Schwedler
HsCD00751821
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 1359nts
Open reading frame: 1 to 1359
ATGTGGTCCCCGGAGCGGGAGGCCGAGGCCCCAGCCGGGGGAGACCCGGCGGGCCTTCTG
CCCCCCGAGTGGGAGGAGGACGAGGAGCGCATGTCCTTCCTGTTCTCCGCTTTCAAGAGG
AGTCGCGAGGTGAACAGCACCGACTGGGACAGCAAGATGGGCTTCTGGGCGCCGTTGGTG
CTGAGCCACAGCCGCCGCCAGGGGGTGGTGCGCCTGCGTCTGCGGGACTTGCAGGAGGCC
TTTCAGCGCAAGGGGAGCGTCCCGCTGGGGCTGGCCACGGTGCTGCAGGACCTGCTGCGT
CGAGGGGAGCTGCAGCGGGAGTCAGACTTCATGGCCAGTGTAGACAGCAGCTGGATCTCC
TGGGGGGTTGGGGTCTTCCTGCTGAAGCCTCTCAAGTGGACTCTTTCTAACATGCTGGGA
GATAATAAGGTTCCAGCTGAGGAGGTCCTTGTCGCTGTGGAGCTGTTGAAGGAAAAGGCT
GAGGAGGTGTATCGTCTGTATCAGAACTCGCCCCTCTCCTCCCACCCCGTGGTGGCCCTG
TCAGAGCTCAGCACCCTCTGTGCTAACTCCTGCCCAGATGAGAGGACCTTCTACTTGGTG
TTGCTGCAGCTGCAGAAGGAGAAGAGGGTCACAGTCCTCGAGCAGAACGGGGAGAAGATT
GTGAAGTTTGCCCGAGGGCCACGTGCCAAGGTCTCTCCAGTCAATGACGTAGATGTTGGG
GTGTACCAGCTGATGCAGAGTGAACAGCTTCTCTCACGCAAAGTGGAGTCCTTATCCCAG
GAAGCAGAGAGGTGTAAAGAAGAAGCCCGCCGGGCATGCCGAGCAGGAAAGAAGCAGCTG
GCACTGAGGTCTCTCAAGGCCAAGCAACGGACAGAGAAGCGCATCGAGGCCTTGCATGCC
AAGCTGGACACTGTTCAAGGCATCCTGGACCGGATCTATGCCTCCCAGACAGATCAGATG
GTTTTTAACGCCTACCAGGCTGGGGTAGGAGCACTCAAACTCTCCATGAAGGATGTCACA
GTGGAGAAGGCAGAGAGCCTCGTGGATCAGATCCAAGAGCTCTGTGACACCCAGGATGAA
GTTTCTCAGACTCTGGCTGGTGGGGTAACAAATGGCTTAGATTTTGACAGTGAAGAACTG
GAGAAGGAATTGGACATCCTCCTTCAGGATACCACCAAAGAACCTTTGGATCTGCCTGAC
AACCCCCGCAATAGGCATTTTACCAACAGCGTGCCTAACCCTAGGATCTCAGATGCTGAA
CTTGAAGCTGAACTTGAGAAACTGTCCTTATCAGAGGGAGGTTTGGTCCCAAGCAGTAAA
TCTCCAAAAAGGCAATTGGAACCGACTCTAAAGCCATTG
Protein sequence predicted based on nucleotide sequence
MWSPEREAEAPAGGDPAGLLPPEWEEDEERMSFLFSAFKRSREVNSTDWDSKMGFWAPLV LSHSRRQGVVRLRLRDLQEAFQRKGSVPLGLATVLQDLLRRGELQRESDFMASVDSSWIS WGVGVFLLKPLKWTLSNMLGDNKVPAEEVLVAVELLKEKAEEVYRLYQNSPLSSHPVVAL SELSTLCANSCPDERTFYLVLLQLQKEKRVTVLEQNGEKIVKFARGPRAKVSPVNDVDVG VYQLMQSEQLLSRKVESLSQEAERCKEEARRACRAGKKQLALRSLKAKQRTEKRIEALHA KLDTVQGILDRIYASQTDQMVFNAYQAGVGALKLSMKDVTVEKAESLVDQIQELCDTQDE VSQTLAGGVTNGLDFDSEELEKELDILLQDTTKEPLDLPDNPRNRHFTNSVPNPRISDAE LEAELEKLSLSEGGLVPSSKSPKRQLEPTLKPL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
chloramphenicol, ampicillin
Growth Condition:
LB with 35ug/ml chloramphenicol and 100ug/ml ampicillin
Comments:
None
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Recommended expression in:
Not Applicable
Vector Name:
pCAG.MCS2.C-Myc
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Mammalian expression vector with a C-terminal myc tag; ampicillin resistance in bacteria; restriction enzyme cloning.
Comments:
Sundquist Lab internal database number = WISP 08-104; Restriction enzyme cloning can be used with this vector - e.g. Xho1, Kpn1 works well
Size (bp):
4813
Parent Vector:
None
Properties:
mammalian expression, multiple cloning site, with tag/fusion/marker
Author Name:
University of Utah
Wesley I. Sundquist
Wesley I. Sundquist
Publications:
PMID:
19525971
Title: Structural basis for ESCRT-III protein autoinhibition.
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3045
3727
primer
forward primer
CAGF forward sequencing primer
1605
1624
primer
reverse primer
CAGR reverse sequencing primer TGTCCCCATAATTTTTGGCAGAGG
1845
1868
selectable marker
AmpR
Ampicillin resistance
3824
4484
tag
c-myc
C-terminal may tag
0
0
