Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
WISP14-114 pCAG OSF ULK3 K44H
Keyword:
"Kinase deffective, Full length ULK3 protein with N-terminal OSF tag, kinase deffective, Sundquist lab internal ID: WISP:14-114"
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pCAG.OSF
Format:
FUSION
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
University of Utah
Wesley I. Sundquist
Dawn Wenzel
Wesley I. Sundquist
Dawn Wenzel
HsCD00751894
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 1416nts
Open reading frame: 1 to 1416
ATGGCGGGGCCCGGCTGGGGTCCCCCGCGCCTGGACGGCTTCATCCTCACCGAGCGCCTG
GGCAGCGGCACGTACGCCACGGTGTACAAGGCCTACGCCAAGAAGGACACTCGTGAAGTG
GTAGCCATACACTGTGTAGCCAAGAAAAGTCTGAACAAGGCATCGGTGGAGAACCTCCTC
ACGGAGATTGAGATCCTCAAGGGCATTCGACATCCCCACATTGTGCAGCTGAAAGACTTT
CAGTGGGACAGTGACAATATCTACCTCATCATGGAGTTTTGCGCAGGGGGCGACCTGTCT
CGCTTCATCCATACCCGCAGGATTCTGCCTGAGAAGGTGGCGCGTGTCTTCATGCAGCAA
TTAGCTAGCGCCCTGCAATTCCTGCATGAACGGAATATCTCTCACCTGGATCTGAAGCCA
CAGAACATTCTACTGAGCTCCTTGGAGAAGCCCCACCTAAAACTGGCAGACTTTGGTTTC
GCACAACACATGTCCCCGTGGGATGAGAAGCACGTGCTCCGTGGCTCCCCCCTCTACATG
GCCCCCGAGATGGTGTGCCAGCGGCAGTATGACGCCCGCGTGGACCTCTGGTCCATGGGG
GTCATCCTGTATGAAGCCCTCTTCGGGCAGCCCCCCTTTGCCTCCAGGTCGTTCTCGGAG
CTGGAAGAGAAGATCCGTAGCAACCGGGTCATCGAGCTCCCCTTGCGGCCCCTGCTCTCC
CGAGACTGCCGGGACCTACTGCAGCGGCTCCTGGAGCGGGACCCCAGCCGTCGCATCTCC
TTCCAGGACTTTTTTGCGCACCCCTGGGTGGACCTGGAGCACATGCCCAGTGGGGAGAGT
CTGGGGCGAGCAACCGCCCTGGTGGTGCAGGCTGTGAAGAAAGACCAGGAGGGGGATTCA
GCAGCCGCCTTATCACTCTACTGCAAGGCTCTGGACTTCTTTGTACCTGCCCTGCACTAT
GAAGTGGATGCCCAGCGGAAGGAGGCAATTAAGGCAAAGGTGGGGCAGTACGTGTCCCGG
GCTGAGGAGCTCAAGGCCATCGTCTCCTCTTCCAATCAGGCCCTGCTGAGGCAGGGGACC
TCTGCCCGAGACCTGCTCAGAGAGATGGCCCGGGACAAGCCACGCCTCCTAGCTGCCCTG
GAAGTGGCTTCAGCTGCCATGGCCAAGGAGGAGGCCGCCGGCGGGGAGCAGGATGCCCTG
GACCTGTACCAGCACAGCCTGGGGGAGCTACTGCTGTTGCTGGCAGCGGAGCCCCCGGGC
CGGAGGCGGGAGCTGCTTCACACTGAGGTTCAGAACCTCATGGCCCGAGCTGAATACTTG
AAGGAGCAGGTCAAGATGAGGGAATCTCGCTGGGAAGCTGACACCCTGGACAAAGAGGGA
CTGTCGGAATCTGTTCGTAGCTCTTGCACCCTTCAG
Protein sequence predicted based on nucleotide sequence
MAGPGWGPPRLDGFILTERLGSGTYATVYKAYAKKDTREVVAIHCVAKKSLNKASVENLL TEIEILKGIRHPHIVQLKDFQWDSDNIYLIMEFCAGGDLSRFIHTRRILPEKVARVFMQQ LASALQFLHERNISHLDLKPQNILLSSLEKPHLKLADFGFAQHMSPWDEKHVLRGSPLYM APEMVCQRQYDARVDLWSMGVILYEALFGQPPFASRSFSELEEKIRSNRVIELPLRPLLS RDCRDLLQRLLERDPSRRISFQDFFAHPWVDLEHMPSGESLGRATALVVQAVKKDQEGDS AAALSLYCKALDFFVPALHYEVDAQRKEAIKAKVGQYVSRAEELKAIVSSSNQALLRQGT SARDLLREMARDKPRLLAALEVASAAMAKEEAAGGEQDALDLYQHSLGELLLLLAAEPPG RRRELLHTEVQNLMARAEYLKEQVKMRESRWEADTLDKEGLSESVRSSCTLQ
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Mutations:
K44H
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Recommended expression in:
Not Applicable
Vector Name:
pCAG.OSF
Synonyms:
''
Description:
Mammalian expression vector with a CBA promoter and N-terminal Flag tag; ampicillin resistance in bacteria; restriction enzyme cloning (Cla1 and Kpn1)
Comments:
Empty vector Sundquist Lab internal database number = WISP 06-65. Myc tag sequence of pCAG.Myc was substituted with One-STrEP-FLAG tag sequence to create this vector. Restriction enzyme cloning can be used with this vector - e.g. Cla1, Kpn1 works well
Size (bp):
4980
Parent Vector:
None
Properties:
mammalian expression, multiple cloning site, with tag/fusion/marker
Author Name:
University of Utah
Center for Membrane Proteins in Infectious Diseases
Kathy Sykes
Brenda Hogue
Center for Membrane Proteins in Infectious Diseases
Kathy Sykes
Brenda Hogue
Publications:
PMID:
19525971
Title: Structural basis for ESCRT-III protein autoinhibition.
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin
0
0
primer
CAGF
forward sequencing primer (5' TCGGCTTCTGGCGTGTGACC 3'
)
1606
1625
primer
CAGR
reverse sequencing primer (5' TGTCCCCATAATTTTTGGCAGAGG
3')
2010
2033
promoter
CBA
Chicken beta-actin (CBA) promoter
0
0
protease cleavage site
Enterokinase
enterokinase (EK) cleavage recognition sequence
0
0
selectable marker
AmpR
ampicillin resistance marker
3992
4651
tag
Flag
N-terminal Flag tag
1858
1881
