Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol:  arnB
Gene Name:  UDP-4-amino-4-deoxy-L-arabinose-oxoglutarate aminotransferase
Original Clone ID:
FLH038332.01F
Keyword:
None
Species:
Pseudomonas aeruginosa
Type:
cDNA
Vector Name:
pDONR201
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
PaCD00007558
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1149nts
Open reading frame: 1 to 1149

ATGTCACTGGACTTTCTGCCATTCTCCCGGCCAAGCATCGGCGAGGACGAAATCGCCGCC
GTGGAGCAGGTCCTGCGTTCCGGCTGGATCACCACCGGGCCGAAGAACCAGGAGCTCGAA
CAGCGCTTCGCCGAACGCCTCGGCTGCCGCCACGCGGTGGCGCTATCCTCGGCCACCGGC
GCCCTGCACGTCACCCTGCTGGCGCTGGGCATCGGCCCCGGCGACGAAGTGATCACGCCG
TCGCTGACCTGGGTCTCCACCGCCAACGTCATCACCCTGCTCGGCGCCACCCCGGTGTTC
GTCGACGTCGACCGCGACACCCTGATGTGTTCGGCGCAGGCAGTGGAAGCCGCCATCGGC
CCGCGCACCCGCGCCATCGTGCCGGTGCACTACGCCGGCTCCACCCTGGATCTCGAAGGC
CTGCGCACGGTCGCCGGGCGCCACGGCATCGCCCTGGTCGAGGACGCCGCCCACGCGGTC
GGCACGANATACCGCGGGCGCCCGGTAGGCTCCCGCGGCACGGCGATCTTTTCCTTCCAC
GCGATCAAGAACCTGACCTGCGCCGAAGGCGCGATGTTCGTCAGCGACGACAGTGCCCTC
GCCGAACGGGTCCGCCGCCTCAAGTTCCACGGCCTCGGGGTCGATGCCTACGACCGCCTG
AGCCATGGACGCAAGCCGCAGGCGGAAGTTATCGAGCCCGGTTTCAAATACAACCTGGCC
GACCTCAACGCCGCCCTCGCGCTGGTCCAGCTCAAGCGCCTGGACGCGCTCAACGCGCGC
CGCCAGGCCCTCGCCGAACGCTACCTGGAGCGCCTCGCCGGCCTGCCGCTGGCGCCGCTC
GGCCTGCCCGCGCACAAGCAGCGCCACGCCTGGCACCTGTTCATCCTGCGCATCGACGCC
GAGGTCTGCGGCCTGGGCCGCGACGCCTTCATGGAAGCCCTGAAGGCGCGCGGCATCGGT
AGCGGCATCCATTTCATCGCCAGTCACCTGCACCACTACTACCGCCAGCGCCAGCCGCGC
CTGAGCCTGCCCAACAGCGAGTGGAACTCGGCCCGCCTGTGCTCCATCCCGCTGTTCCCC
GACATGCGCGACGACGACATCGAGCGCGTCGCCCGCGCCATCGAGGAGATCCTGGAGAAA
CGTCGAGGA

Protein sequence predicted based on nucleotide sequence

MSLDFLPFSRPSIGEDEIAAVEQVLRSGWITTGPKNQELEQRFAERLGCRHAVALSSATG
ALHVTLLALGIGPGDEVITPSLTWVSTANVITLLGATPVFVDVDRDTLMCSAQAVEAAIG
PRTRAIVPVHYAGSTLDLEGLRTVAGRHGIALVEDAAHAVGTYRGRPVGSRGTAIFSFHA
IKNLTCAEGAMFVSDDSALAERVRRLKFHGLGVDAYDRLSHGRKPQAEVIEPGFKYNLAD
LNAALALVQLKRLDALNARRQALAERYLERLAGLPLAPLGLPAHKQRHAWHLFILRIDAE
VCGLGRDAFMEALKARGIGSGIHFIASHLHHYYRQRQPRLSLPNSEWNSARLCSIPLFPD
MRDDDIERVARAIEEILEKRRG

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1149
Mutations:
None
Discrepancy:
g485c,c486g,S162T; gaa163ana
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable