Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
7773
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00762858
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5022nts
Open reading frame: 1 to 5022
ATGTTTAAGTGGGTTACGCCGGCCTCAACGGCGACGTTGTCCCGCTGCACACTGCCAGCA
ACAACAGCAGCCACAACAACAACAACGGCGATGGCAGCAACGAGAACAGCAACGACAACG
ACGAGAACAACAAGACCACAACTACTTTCCATCGCTTTAACAAGTTTAATAATAATAGTC
GCATCATTTGTGCCGACAACAAGTGGATTTAGATCAATTGAAACGAATGGCGGTGGTCGC
AAGCTCTTTGGGGGCTATCGCATCACACCGAAGCACTGCCGTGCCACCAAGACGCTGCCC
AGCTCGGATCCACGGGCCAATGGACCCACCATCTGCATGTTTAACCACGAGTGCGCACAG
CGGGGTGGCGAGGTGGTGGGCGCCTGCATGGACGGCTTCCTCTTCGGCGCCTGCTGCCAG
ATCCCGCCCACCCACGAGTTGGCCAGCACGCTGATTAACGAGGCGCAGAACGCCTACTTC
CAGCAGCACCAGCAGCAGACGAAGCTCCAGCAGTCAGCAGCCCAATCCTCGTTCGAAAGC
TACGGCGAACAGCAACAGTCATTGAGCGAGGAGCAGGTGGCACAGCAGCCGTCGCAGAAC
ATCTACGATCAGCAGAACCTGGACAAGGTGTACCAGCAACTGGACAGCAGCAGTAGCATC
AGTCCACCCAACGGAGCCTACGGCGATGAGCCGCAGCAGCAGGAGTATCAGTCGGAGTCG
GAGCAGCCCGTAAGGGATGAGAATGCGTATCCTACCAGCAGTTCATCCACCGAGGCCACT
CAGTCGCAGTCCTCCTCCGCCAGTGTGGAATTTGAGCAGGAGCCCTCCCAACCAGCAGAT
GCCTCCAACGATCAGACCACTCAGAAGATCAACAAACAGCCCGTCCAGCCACCCAACTTC
CACGTCCACAAGCACTCCGTAACCATCAACTCACCCTCTTCGCCGCCTCAAAACGATGAC
TTCGTAATGCAGGTGCTAAGCACCTTGCCACCTGAGCATGCGGATGACCATCATATTGTC
TTTACCACCGAGGTGCCCACCAAGATCACCAGTGGATTGCAGGATCAAACGAGCTCTGAG
TCCAACTCCTTCGAAGAGGTGTCTTCGACGCCAGCAGCCACCCAAAAACCGAAACCCAAG
CCAACTCAAATGCCCACTCAGAAGACCACTCAGAAGGCCACGCAGAAGCCCACTCCAAAG
CCCACACAGAAAGCCAAGCCGAAACCAGTTCCCCAACTGGCGGAGAGCATGAAGCGACCC
ATCCAACAGAAGCCACAGCAGGTGGCGAAACCTAAGCCAAGCCCTAAGCCTGCCCAGTCC
ACAAACAACCACCACCACAACCACTTGATCCTGGACGGTGGCGAGTTCACGCACAGTGAC
ATCACCCACCCCGGAGCAGATGCCGATCTCGTGGAGGATCTGCAATTCTCCACTGGCTAT
GGCCCCCAACCTGTGTACGCAGAACCACCGAAGCAGCAACAGCAACAGCAACCTGCTGAG
CAGAGCTACATTTCCTCCAGCACTAGTGCGAAACGTCCAACGACAGGTCATAATAGTCCC
ACCACTGTTTCGTCAATTACCACCCACGTAGACTCTATCGAGTCCATCATCCTGCAGCTA
AACAATACCAGTCATGGTCCCAGCTATAATGTTGTGAGCCAGCAGACCCCATCATATGGA
TATCCTGGAGCAGCAGTGGTTCAGACTGAGCCAGCAGCCCAAAATCCGACCTTCTACCAG
GAGAACGAGTCGGAGAAGGTCCAGGAGTCGGACTCGCAGTCTGATTACGGTTACACCACC
ACAGTTAACTACGAGTCTTTCTACGACAAGGTTTCAGATGAGCAGGATGCCTCCGCTGCT
GTCAGTCAGTCTGCTGAGATGCCCACCGCACGTCCTGGATACGGAGAAGATGTTTCCGCT
GTGCTGGAGGACCATACGATGCCGGCCAATGGTTACCACGATGCCGAGGCTCCAGTTGCT
CCACAGACTTCGGAGTTTAACAAGATGCCAGTGATGGGCATTGCCTATCCAGTGGATATG
TCTTATATGGAGGAGGAGGGAAATCTTCCGGCCACTGCAGCTGGTTATGGCCAGATGAGC
AGTGACTCCTACGAGGCGAGCACTGAATCCACCTACCAGAAGTTGTCAACGGTCCAAACG
GAGGAGCCGCAGCCAACTTACGTGCGCCCCACAACCAATGCCAATAAGCAGAATCGCCCA
GTGGCCTCCTACATTGGAATGGTGACAATGCAGCACTATAACCCGCAGCCCGGAAATGGT
GACTACCAGGCTCAAGTGCCCCCCGAAGTATCCGTGTCCTCGCACACCACCAAAGTCCAG
GAGCAGATGGATGAGACCTCGAATGGCTATCAGCAATCGGAGACCACTTCTGGCTATGTT
TCGCCACCAACTGCTGTTCCTGCGCCAGCACAGCGGCCTCAGTATGACGCTGTCCAGGGT
GATGCTTCCTCGGAGCGACCTGTCCTGGTGACGGCCAGTCCCAGGCCCCGACCCAAGCCC
AGTACCAAGCGACCTGCCGTGAAGAGACCCATCAGCGGCGAGAGCACCAAGAAGAAACCT
CAGCCACAGCCAAGTGCCGGTGCCTACAACCAAGAGAAGATCAGCGAACATAGCACAAGA
AAGCCAGTGTCCAATGGATACGATAAGGTACCCGAGTCGCCCATCACACACATTCAGATT
AAGAAGCCATCGGCAACTCAACACAAGGAACAAGAGCAGACGGGATACCCCAGACCTGCC
AGCCCTGCGGGATACGAACAGACAACTGCCGCAGCTCCTGCGCCAGCTGCTCCTTCTCTC
AACTACGACAAGCCGGATGCTCCTCCCAGCCAGTACGACCAGCCCTCGGCTCCATCCGCA
AGTTACGATCAGTTGGCGCCGATGCCGTCTCTCAACTACAACGAGCAGCACGCTAGTTCA
CCAGGAAGGAAGCCGTCAACGGCCAAGCCCATTTCCACCAGCTATGTGACCGGACCCAGT
ACCCCACGACCCCCAGCCACCGTTGATTACCACTATGACAATGTGCCACCACTGTTCATG
GCGGACGACAAGCTGGATGCCTTCATCCAGAGCACTGCGGAGAACATTGTCGGATCAACC
CCGGGCAACTATCAGCCTCCGCTGGTGGCTACCGCATCGACGCCCGCTTACGCCCATAGA
CCAACCAGCAGTGGTAGCTATGGCCATAAGAAGCCTGGCTTCGTCCAGATCAATGGAACA
CCCAAACCTCCCAGACCGACGGTGCTCATTACGCCCAAGCCCACTGCCATAAATCTTGTG
ACCTACAGCTCTTTGAGCGACGATAGCAACAAGCTGGCCTCCAGCACAAGTTCTTATGTG
ACCGGAAGACCGGGAGTACAGGGAGTAAGTTCCAACGACTTCAAGGATCCCGGATACTTT
GGCAGTAGCCCCGTCCATGTGGCATTCACGCAATCCACCACAGAGGCCGTCTACGCAGTG
CCCTCCGATGACAAGCCAGCATTCCCAGGATACTTCGGGCCAACGCCCTCGTATCCCGCC
TTCTCAGTTCCCGGCGAGAAAGTGGGCCAGAATGTTATGGAGGAAACCTACACGTCGCCC
AATGATTTCGTCAACTTCCCGCCGGTAAGGAACCCCAATCTGAACATGTCGGCTGCCTCC
AGCGCAGTGACCAGCGATCTCGACCTCTCCACACCCGCCTTTGTGGAGGATGTGGTGCTC
AAGGACAAGATGCACACCCTGGTTCACAAATTGGTAGCCTCGCTGCAGGGTAACTTTGAG
GCCCTGGCCGATATGATCGAAGAGCCGGGAAGCAACAAGACCGTGGCCACCTACCAAGCC
GGAGCAGGAGGAACTGCCAAGCCTGTGAGAGTAGTAACCACACGGAAGCCTGTGAGGACA
GCCACCACAACCAGGCCCAAGGTAACCACAAAGAAACCAGTCACCCGGGTCACGACAAAG
GCTCCCAACAAGAAGACCTCCGCTGTCAGCACCACCACTCGCAAGCCCGCTACGCGTCGC
ACCACCGTTGCCGCAAAGGTAACCACCACCACTCGGAGACCGGCGACCAAGAAGCCAACT
CGCAGGGTAAGCAGTACCGTGAAGACCACGACCGTTAGTTCGGCTAGACCGGCGGACGAC
GAGATCGTGGACGAGGAGGACGAAGAGGACGTTAACCCGAATCCCAGCGACAACGAGATT
GACCAAGGAGCTACTTTGAGCTCGTACGGCGGAGCCAACGGTCGGAAGATTCAGTGCGGT
GTACGGCCCCATGTCAAGTCCGGCCGCATTGTCGGAGGCAAGGGCTCCACCTTCGGCGCC
TACCCCTGGCAGGTCCTCGTCCGGGAGTCCACCTGGCTGGGCCTATTCACCAAGAACAAG
TGCGGCGGCGTCCTAATCACCAGCCGCTACGTCATCACCGCCGCCCATTGTCAGCCTGGA
TTCCTGGCCTCTCTGGTGGCTGTTATGGGCGAGTTTGACATTTCCGGCGACCTGGAGAGC
AAGCGTTCAGTGACCAAGAATGTCAAGCGCGTCATTGTCCACCGGCAGTACGATCCAGCC
ACGTTTGAAAACGATCTGGCCCTGCTGGAACTGGACAGTCCAGTGCAATTCGATACCCAT
ATAGTGCCCATTTGCATGCCCAATGATGTGGCGGACTTCACTGGACGCATGGCGACAGTG
ACGGGATGGGGACGCCTTAAGTACGGAGGTGGAGTTCCCTCCGTTCTCCAGGAGGTGCAG
GTGCCGATTATCGAGAACAGCGTTTGCCAGGAGATGTTCCACACCGCTGGACACAACAAG
AAGATTCTCACCTCCTTCCTGTGCGCCGGCTATGCCAACGGACAAAAGGACTCGTGCGAG
GGCGACAGTGGCGGTCCGCTGGTGCTGCAACGACCGGATGGACGCTACGAGTTGGCCGGA
ACCGTTTCCCACGGCATCAAGTGTGCGGCGCCGTATCTGCCAGGTGTCTATATGCGCACC
ACCTTCTACAAGCCGTGGCTGCGCAGCATAACGGGCGTGAAA
Protein sequence predicted based on nucleotide sequence
MFKWVTPASTATLSRCTLPATTAATTTTTAMAATRTATTTTRTTRPQLLSIALTSLIIIV ASFVPTTSGFRSIETNGGGRKLFGGYRITPKHCRATKTLPSSDPRANGPTICMFNHECAQ RGGEVVGACMDGFLFGACCQIPPTHELASTLINEAQNAYFQQHQQQTKLQQSAAQSSFES YGEQQQSLSEEQVAQQPSQNIYDQQNLDKVYQQLDSSSSISPPNGAYGDEPQQQEYQSES EQPVRDENAYPTSSSSTEATQSQSSSASVEFEQEPSQPADASNDQTTQKINKQPVQPPNF HVHKHSVTINSPSSPPQNDDFVMQVLSTLPPEHADDHHIVFTTEVPTKITSGLQDQTSSE SNSFEEVSSTPAATQKPKPKPTQMPTQKTTQKATQKPTPKPTQKAKPKPVPQLAESMKRP IQQKPQQVAKPKPSPKPAQSTNNHHHNHLILDGGEFTHSDITHPGADADLVEDLQFSTGY GPQPVYAEPPKQQQQQQPAEQSYISSSTSAKRPTTGHNSPTTVSSITTHVDSIESIILQL NNTSHGPSYNVVSQQTPSYGYPGAAVVQTEPAAQNPTFYQENESEKVQESDSQSDYGYTT TVNYESFYDKVSDEQDASAAVSQSAEMPTARPGYGEDVSAVLEDHTMPANGYHDAEAPVA PQTSEFNKMPVMGIAYPVDMSYMEEEGNLPATAAGYGQMSSDSYEASTESTYQKLSTVQT EEPQPTYVRPTTNANKQNRPVASYIGMVTMQHYNPQPGNGDYQAQVPPEVSVSSHTTKVQ EQMDETSNGYQQSETTSGYVSPPTAVPAPAQRPQYDAVQGDASSERPVLVTASPRPRPKP STKRPAVKRPISGESTKKKPQPQPSAGAYNQEKISEHSTRKPVSNGYDKVPESPITHIQI KKPSATQHKEQEQTGYPRPASPAGYEQTTAAAPAPAAPSLNYDKPDAPPSQYDQPSAPSA SYDQLAPMPSLNYNEQHASSPGRKPSTAKPISTSYVTGPSTPRPPATVDYHYDNVPPLFM ADDKLDAFIQSTAENIVGSTPGNYQPPLVATASTPAYAHRPTSSGSYGHKKPGFVQINGT PKPPRPTVLITPKPTAINLVTYSSLSDDSNKLASSTSSYVTGRPGVQGVSSNDFKDPGYF GSSPVHVAFTQSTTEAVYAVPSDDKPAFPGYFGPTPSYPAFSVPGEKVGQNVMEETYTSP NDFVNFPPVRNPNLNMSAASSAVTSDLDLSTPAFVEDVVLKDKMHTLVHKLVASLQGNFE ALADMIEEPGSNKTVATYQAGAGGTAKPVRVVTTRKPVRTATTTRPKVTTKKPVTRVTTK APNKKTSAVSTTTRKPATRRTTVAAKVTTTTRRPATKKPTRRVSSTVKTTTVSSARPADD EIVDEEDEEDVNPNPSDNEIDQGATLSSYGGANGRKIQCGVRPHVKSGRIVGGKGSTFGA YPWQVLVRESTWLGLFTKNKCGGVLITSRYVITAAHCQPGFLASLVAVMGEFDISGDLES KRSVTKNVKRVIVHRQYDPATFENDLALLELDSPVQFDTHIVPICMPNDVADFTGRMATV TGWGRLKYGGGVPSVLQEVQVPIIENSVCQEMFHTAGHNKKILTSFLCAGYANGQKDSCE GDSGGPLVLQRPDGRYELAGTVSHGIKCAAPYLPGVYMRTTFYKPWLRSITGVK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
