Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
7881
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00763040
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5472nts
Open reading frame: 1 to 5472
ATGGATCGCTACGACATGGAGGACGTCGTTGTGGCGCCCCCTGCCGAGTGCAGTTCGCCG
CTCAAGGAGTGGCGCCTCAAGCGCCAGGCGGGCACACTAGACCGCTCCGATATCATCGAG
TTCTTAAAGCGCGAGTCTCCCAAGTATTTTGATTACCAGACTAGCGCTACAGTTAAAGAT
ACCAATGTGATTACTCTAAAGTGCATGTTTAAGGAGTCGCTGGCCAAGGAGGAGATCATC
GATGTCGTTGTGGAGTTTATGCTGGGCGACAATCCTTCCTCCGCACTGGAGAAGCTCCGA
CTGGAGGGCAACACGGCCACCGTCTGTGGCAAGGTCTTTAAGAACGGGGAGCCCACCTAC
AGCTGCCGCGAGTGCGGCGTCGATCCCACCTGCGTGCTTTGCGTCAACTGTTTCAAGCGC
TCGGCGCATCGCTTTCACAAGTACAAGATGTCCACTTCCGGCGGCGGCGGCTGCTGTGAC
TGCGGAGACGACGAGGCCTGGAAGAAGGATCAGTACTGCGAGCTGCATCTGGCAAATCGC
AAGAATCCGCTGGAGAGCAAGATCCTGACCGACGCAGTACTTGAGCGGGTTGAGATCTGC
TTTGGGGCAATCCTCGCCTTCTGCGTGAGTTATCTGGAAATCGAGCCAAACGCCAGTCTA
CAGTGCCTGGATGGAAACGTGGAAGGCGGCCAAGTGGACGGAGCGCAGTATTGCACTGTG
CTCTACAACGACGAGTCGCACACGTTCGACCAAGTCATTCAGACGCTGACCAAAATTGCC
AAGTGTCGGGCAAAGGACGCCATGGAAATCGTGGCGGCAATTGATCGCGAGGGACGAGCG
GTGGTCAAATGCGATACATTTGAGGAGTGCAACAAACTAAAGGTATCCATCGAGAACCAA
ATGATTCTGCCGACCTCCCTGGTGTCGACGGCACGCAACAATCAGTCACTCAGGACATCG
GTGCTGCACATCGGGGCCGTGGCCTGTCAGCAGTTTGCCCTGCAGCTTCTCGGCTGGTTC
CAGGAGTTCCTGGTGCGACACTACCTCTTCCGGAAGACGTTCTCCGAGTTGGTGCAGCGC
AAGCAGGAGACATTCTGCATCCGGCACATCCTTGAGTACGACGTGAAGCTTTGGAAGACG
GCGCGCACCTGCTGGCACCGCCTCCTGATCTCTGGTATGCTTATGGAGTACGACAACAAA
ATGATCCTAGCCCAGGAGTTTTCGCGACGCTACGCCACTATTGTGGAGGACTTTATCAGC
GATGATCATGATCACGCCTTTTCGATTGTGTCGCTCAGTGTCCAGCTGTTCACGGTGCCC
AGCATAGCCCATCATCTGATCGCTCATGAGGGCATCTTTGACAAGCTGCTGCACACGTTC
TACCACGTTGCCATCGAGAAGTTCATTCGTAACAAGACGCTGCACTTTAGCAAGAACATC
GCCAGCCTCACCTTCTTCAAGCGGGCTAACTACATCCTCTACGATCTGCGATACCTGCTC
AGCCTAAAGCCGGATGTGCTGAGCAATGATTTACGAAACGGTTTCCTAGAAGGCTGCCGG
GCTTTGATGCGCGTTCTAAACGTGATGCAGGGCATGGAGTCGATGACCAGACAGACGGGC
CAACATATGGACTACGAGCCAGAGTGGGAGTGCGCCTTCAACCTGCACATCAAGCTGGCC
ACGACCATCTCGCAGGTGATTGATTGGGCGTCCGGCGATGTGAAGCTGCTGCGTAAGCTC
TACAAGATGACAATGCGGGCGCTGGTGAGCAACAGCTTTATTGTGGGTGGCGAGAAGGTC
ATGCAGCCGAAAAAAGTGGCGGACCATGTAGCCAACTGTCTAGTTTACGACATATCAGTG
CAGCCGGTATCCATCCACTTGCCTTTGTCGCGTTTCTTCGCCGGAATCTATCTGCACTTG
GGTGCCCACGATTTAACGTACGACGGGCTGCAGACGGAAACAGAAGCGTTGAGCATTAAG
CTAACGCCCAGGGAGATTATAGAACCGGTTTTGTGCACCCAAGCAATGATCGCCCAGGTG
GGCGCAGGACTGTGGCGCCGCAATGGTTACACCCTGCTGCATCAGCTCTACTTCTACCGA
AATGTACGTTGCCGCGTGGAGATGCTGGACAGGGACATAGCCTGCCTGCAGATAGGCGCC
TCCCTAATGGAGAGCAACGAGTTCCTGATCCATGTGTTGAATAGGTTCAACACGATCCCC
TGGTTACAGGAAAACTACTGGTCCCTATTGTCGGGTAATGAAATGAACGATGATATCATT
CGAGAGGCGTCAATCTTTGACGAGTTCCTGGAACTTCTGATCGTGATCATTGGAGAACGT
TGGATGCCTGGCGTTTCGATGGTGACCGAAGAGGATCGCCTGCGCAAGGAGATCATCCAG
CTGTTGTGTATCAAGCCATACTCACACTCGGAGTTGAGTCGCGCCTTGCCAGATGGCAAT
AGCGGAAATAGCGACAACGTTTTCGAGGAAGTTATCAACACGGTTGCCGTGTTCAAGAAA
CCCGTTGGAGCCGACAGCAAGGGGGTGTACGAACTAAAGGAACATTTGCTTAAGGAGTTC
AACATGTACTTCTATCACTACACCAAAGAGGACAAGTCCAAGGCCGAGGAACTGCAACGG
GAGCGCCGAAAGGCCAAGAAGCAGCTTGTCTGCTGCCCGCCACCAATGTTGCCAAAACTA
ACACCAGCCTTCACACCCATGGCCAACATCCTGCAGTGCCCTGTGTTCCTCAACATCTGC
AGCTTGATTATGGAAAGGGCTTTAAATGCTTACAGTCGCAGCTTTACCGAATCGCATCTT
CAAAAGGTCCTTCACCTGCTGGGTTATGCCATTCAGGAGGAGCTTAGCGAGCACTATCCC
TTCTTGAGCTTCTACGAGCGTTCCCAGGAGTACGGAATTCTGGAGAAGTTGGAAGAGCTC
GCTCGCTGCCCGAGGTTGGAGGCTCATTATGACTTTGTGCTGTGGACCATCGAGCGCTTC
AAGCAGCTGCAGGCCAAGCAGGCTCCCAGTGATGGTAGAGCAGGACCCAGTTGCTCTCAG
CAAGGAACCGGAGGGAAATTGTCACTCAGCGCTGAGGAGCAAGCGCGAGAGGAGAGGGAG
AATCGTGCTCGTCTAGCCGCCGAGCGTCGAGCCCACATTATGGCCCAGATGCAGAAAGCC
CAAAAGTCGTTCATAAGCAGCAATGCAGAAATGTTTGCGGACACCGAAAACGAAACACGC
AAGGAGTCAGCATCCACGGGTCCAATGGATTGGGAGGATATTCCCCCCGAGGAAGAACAG
GGAGCAGTGGCCCTTGAGTCGAAGGTAGCCTGCTTGGGGCCGGACCGCAAGTTTTATCAC
GGCACCGATGATACCTTTAAATGCATCCTCTGCTTCGAGAACTGTGCCATTAGCCGTGGG
GGCCGGCAATTGGTTAGCTCTGCCTTTGTCCAAACCTCGCGTGTGATCTTCACTACGCCC
AATTTGCGTAATAGTCAGAGCGCACTGCACATTAGTTGCTGCGGTCATGTGATGCACTAC
AGCTGCTGGCTGGAGTACTTCACCAACGAGGAGTTCAAGGAGCTGCGTCGTCCGCACCGC
AATCGTGCGGCCCTAGCCCAGGCCGCCAACGTGGAGTTCCAATGTCCGTATTGCCGAACC
CTAAGTAACGCTATAATACCGGTAACCGAAACGCTGCCCGCTTTTTCGGCGCCTCCTTCA
CCGAACGAAAGCTATTTACCATTGGACAGTTTCGTGGAGATCATGAGCACACTGGCCATC
GAACTGGGTAACGTGAAGGACCACGAATTGACAACACTTCCAAGTGTGTCCAACATTTTG
CGCTTGTCCGGCGTGGTCGGTGGCTTGGCTCAATTCGAGCGGAGCGTGCAGCTCATCAAG
AATCCTCCTAGACTGCATGCTGACTACATCGAAGGAATTGAATTCCTTAAAAAAGCCCTG
CTAAATACCATGAAGATTCAGCAGTCACATCTAAAGGACCATCCAGCCATCGAAAGTATC
GAAATGGTACCCATACTGTGGGACAGCTGCAGCTACACGCTGCAGGCACTGGAGATCTAT
TTGTACGCGGTCGAGAAGCCATTGAAGGCCGAGCTGTCAATGCGCCACCAGAGCTGTGCC
AGGAACTTGGTGAGGGCTTGCTCACGCAGTTCGGCCTTGGAATGGGAAACTGATCTTCCA
TTGCTTCCGCCGATGCGTTCCCAGGCGGAGTTTTCCTCTAGACTGCTGGATACAATCTTC
AATCAAAATGACACCAGTGTGCTCGAGTGGGACTGCTTCCGCGTGCTAGTGCCGTTCCAG
TTTGGAGTGCTCAATCTGATGGTACCCGAGAAGGGATATAAAACAATCATACCCAGCGGC
AGCATGTTCGACTTTTACATCATGCAGACTATGTTCCTGGCCCAGCTGACCAAGGCGGTC
CTCTGCTTTGACGTGGAGAAGGAGAAGGCCAAACGAGCGGAGAAGGCGCCCAATTCTGAA
CTTACACAGTTGGATTACATAGAGCAGCTACCGTCACGGATTCGGGACAACATGATCGAC
TTTTATCGTCGCTACAATATCCCAGCGAGAGTGCTGCAGAAGACTAAACAGAAACAGCTG
GTGGAGGAAGAATCGGAAGAGAATCAGGGTCATGGGCAAACAGTGGTGATACCCTGTGAA
TCGCATCATTTGGCCCTTCTTCTCGAGTACGTGCAGCGACAGATGAGCTCGTTCCTGCGC
TGCTCCTGTCTCTTCTATCGCTTTCTCACCGATGTGGATTTCCCAGACACATTTCCCACA
GATCAACCGGATCGCTTTGATCTGATGTGCCAGTACTTGGGCCTGGACCCCATGCTGGGT
GTCTACTTTGACATGGAGACGGTATACGCCACCATGATGCACTCGTTTGCCTCGCATCCT
CACATTGACCGTGAAGTGGAACAGCGTTGTCAGCCTGACGCCCGTCGTTCCCTTCAGGTG
GAACCTTGCCTGCGCCCGCTGCCCCGCTTGAAGGTACTGTGTGATGACTTCAGCGATTTG
ATCAACAGCGTCTCGGACATCTTCTGTCCGAACAACGAGCGCGAAGAGATGAAGACACCA
ACGATGTGTCTGATATGCGGCTTAATCCTGTGCGGTCAGTCGTACTGCTGCCAGCCAGAG
CTGGGCAAGGTCTCGGTGGGCGCATGCACTCATCACGCTCACGCTTGTGGCGCCGAAGTG
GGCATCTTTCTGCGCATTCGCGACTGCCAGGTGGTGTACCTGGGCCGTGGCAAGGGGTGC
TTCGTCCCGCCGCCATATTTGGACGAGTACGGCGAGACGGATATGGGTCTGAGGCGCGGT
AATCCGCTGAGACTGTCCCAGGCGGCTTACAGAAAGATCTACCTGCAGTGGCTGGGGCAT
GGGCTGCACGGCGAGATCGCCCGTCTGAACGACAACGCCAATGTGGCAGCGGCGGCGCAG
TGGCATCACATG
Protein sequence predicted based on nucleotide sequence
MDRYDMEDVVVAPPAECSSPLKEWRLKRQAGTLDRSDIIEFLKRESPKYFDYQTSATVKD TNVITLKCMFKESLAKEEIIDVVVEFMLGDNPSSALEKLRLEGNTATVCGKVFKNGEPTY SCRECGVDPTCVLCVNCFKRSAHRFHKYKMSTSGGGGCCDCGDDEAWKKDQYCELHLANR KNPLESKILTDAVLERVEICFGAILAFCVSYLEIEPNASLQCLDGNVEGGQVDGAQYCTV LYNDESHTFDQVIQTLTKIAKCRAKDAMEIVAAIDREGRAVVKCDTFEECNKLKVSIENQ MILPTSLVSTARNNQSLRTSVLHIGAVACQQFALQLLGWFQEFLVRHYLFRKTFSELVQR KQETFCIRHILEYDVKLWKTARTCWHRLLISGMLMEYDNKMILAQEFSRRYATIVEDFIS DDHDHAFSIVSLSVQLFTVPSIAHHLIAHEGIFDKLLHTFYHVAIEKFIRNKTLHFSKNI ASLTFFKRANYILYDLRYLLSLKPDVLSNDLRNGFLEGCRALMRVLNVMQGMESMTRQTG QHMDYEPEWECAFNLHIKLATTISQVIDWASGDVKLLRKLYKMTMRALVSNSFIVGGEKV MQPKKVADHVANCLVYDISVQPVSIHLPLSRFFAGIYLHLGAHDLTYDGLQTETEALSIK LTPREIIEPVLCTQAMIAQVGAGLWRRNGYTLLHQLYFYRNVRCRVEMLDRDIACLQIGA SLMESNEFLIHVLNRFNTIPWLQENYWSLLSGNEMNDDIIREASIFDEFLELLIVIIGER WMPGVSMVTEEDRLRKEIIQLLCIKPYSHSELSRALPDGNSGNSDNVFEEVINTVAVFKK PVGADSKGVYELKEHLLKEFNMYFYHYTKEDKSKAEELQRERRKAKKQLVCCPPPMLPKL TPAFTPMANILQCPVFLNICSLIMERALNAYSRSFTESHLQKVLHLLGYAIQEELSEHYP FLSFYERSQEYGILEKLEELARCPRLEAHYDFVLWTIERFKQLQAKQAPSDGRAGPSCSQ QGTGGKLSLSAEEQAREERENRARLAAERRAHIMAQMQKAQKSFISSNAEMFADTENETR KESASTGPMDWEDIPPEEEQGAVALESKVACLGPDRKFYHGTDDTFKCILCFENCAISRG GRQLVSSAFVQTSRVIFTTPNLRNSQSALHISCCGHVMHYSCWLEYFTNEEFKELRRPHR NRAALAQAANVEFQCPYCRTLSNAIIPVTETLPAFSAPPSPNESYLPLDSFVEIMSTLAI ELGNVKDHELTTLPSVSNILRLSGVVGGLAQFERSVQLIKNPPRLHADYIEGIEFLKKAL LNTMKIQQSHLKDHPAIESIEMVPILWDSCSYTLQALEIYLYAVEKPLKAELSMRHQSCA RNLVRACSRSSALEWETDLPLLPPMRSQAEFSSRLLDTIFNQNDTSVLEWDCFRVLVPFQ FGVLNLMVPEKGYKTIIPSGSMFDFYIMQTMFLAQLTKAVLCFDVEKEKAKRAEKAPNSE LTQLDYIEQLPSRIRDNMIDFYRRYNIPARVLQKTKQKQLVEEESEENQGHGQTVVIPCE SHHLALLLEYVQRQMSSFLRCSCLFYRFLTDVDFPDTFPTDQPDRFDLMCQYLGLDPMLG VYFDMETVYATMMHSFASHPHIDREVEQRCQPDARRSLQVEPCLRPLPRLKVLCDDFSDL INSVSDIFCPNNEREEMKTPTMCLICGLILCGQSYCCQPELGKVSVGACTHHAHACGAEV GIFLRIRDCQVVYLGRGKGCFVPPPYLDEYGETDMGLRRGNPLRLSQAAYRKIYLQWLGH GLHGEIARLNDNANVAAAAQWHHM
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
