Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
11013
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00763196
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6018nts
Open reading frame: 1 to 6018
ATGAATGATTTACGTCAGCAGCAGATGGTAGCAGCGACATCGTCATCAGGATCGGAATCG
GGAACAGCGGTAGAATCGGCAGCCGCCACGTCCACAGCAGGATCAGCCGGAGCAGCAGGT
CGCCCCCAGTCTAACTGTAGCGCTAATAGCAACGCCAAGTCAGTGGCAGCGAGCAGCACG
AGCGAGGAGGAGCAGCGAGTTAGCTCCACGTCGTCGCCAGCCCAGCGGGATCAGCAGCTG
AATGCAGATCGGGACCGGGAACAGGAGCCAGAGCCACAGCAGCAGCAGCAGCGGGAGGAG
GCACTGCAGCACCAGCATAATCAGCCCGGCCATATAACCAGCACCACAGCGTCGCCTCCG
CCGACGCTGCCACCGCCGACGACGCCGTGCGACGATGCCCCAAGCACAACTGGAGCATCT
GCATCGGCCAGCTCAGCATCCGGAGAAGCACCATCAGCAGCATCAGCAGCGGGAGCAGCA
GGTGGACCGATGGCCGCTACGGCGCTTGAGGTAGAGAGCGAGGAGCGGGATGGACATAAG
ATCATCCTGAAGCTCTCCAAGCACGCCAATCCCAACTCAAACGCAAATGAATCTCAGCCG
GGCGGAGATGAGAGGCGCGTGGAGCCCCTGCGCATTCAGCTGCCCTGCGGCGGAGCGGAA
GGAGGCTTGGTTGCGAAGCAGCAAGATGCCTTCGATGCGGACGCCTCTTCCTGCTCCTCG
TCGTGCGCCGAGGACGAGGTGGCTACCACCCTCGGCCAACAACTACGAAACACCCCGCAC
ATTGTGCCAAAGCTGACCATCCGCGCCGCGAACGAGAGGCGCGTAGGCAGCGTGGTGCCT
AAGCTGACCATCAAGTTGCCTGAGAATCCGGCTGCTTCCGGCTCCAACTCCAACTCTGGA
TCGTGCTCAGCAGCTGTGAGTGGCGCCCAGTCAGCTATGCCCGCCAAGAACGATGCCCAC
CTGTCCAGCCTCTCGCCCGCATCCGCCTCGTCTTCATCTGCCTCGTCCTCCTCCTCATCG
TCGTCGTCGTCCTCACTGGCGGAGATGCAGACGGTGCCCAAGCTGATGATCAAGACAACG
CTAGCCGGTAGTAGCTGCATCAGCAGCAGTGAGGAGCTTCCGCAGCAGCAGCAGCAGATA
CCAAAGTTAACAATCAAGACAGGTGGCGGCGGTCAGGAGCACGTGCACACGGTCATAATG
ACGCACGACCTAAACAACGCCCAGAGCATCCCCAAACTCACCATTAAAACCAAATCCATA
GAGATGATCGAGGACGAACAGGCGGCTAAGCTGGAGCAGCAATTGCAGCCGCTGCCCAAG
CTGACCATCAAGAACTTATGCTCACCGAAGCACAAGGTGCGTGCAGTGCTCGAGGAGAAG
CCACCAACGGCGGCCTCCAAGCTGGCTATCCCAAAACCCACGCCCAATCCCACGCCGGCT
CCCATGACGAATGGCGGCGAGTCGAACAGCAGTAGTCAGGAATTCTGCGGCTTCTCCGAC
CCAGACGCAGATATAGCTGCGCCAGCCTCGGACGACGTTCGCCGCAATTCGGACGACATG
GTGATCGACGATTCGCTCTCCAAGGAGCACGATCCCAAGATTTTTCACAATCTACCGCCC
ATGCCCGCCAGCAACGGCATAGCCAGCGGTGGGAGTAAGGCCAGTAAAGCCAGCAAGTCT
GCCCAATCCCAGCACAACGTTGTGGACATGGTGGACCTGACCTCGTCACCGTCGCCGGGC
TCTTCGCCAACGCATGTGCCTAACAACTTCACGGGACGCATTTCGCCGAAAGGGTTGCTC
ATCGACTGCCTGCGGATGCAAACTGCGATCGGCTGTTATGTGCCCGAGGAGGCCGACAGT
CGCGTTAGGCCAGCTGCGTCGCCCAATTCGAACATATTGCTTAGCCAGCTGACGGCACCG
GCTAAAAGCTTCACAACGACCACGCCTAAAAGCAAATCCAGCAAGTATCCCCAGCTAACG
GAGCGCCTGATGGCGAATGGAAGCGGTACCGGTGGGGGATCTGTTATTGCTGCAGAAAGC
GAGGCAGTCGTTGGTCCCTTGCCCGCCGCGAATCCCGCCCAATCCATAATCGAGTCCATT
GAAATCCTGGACACGCCTGATGGCAGCCCGAGGGTAGCCTACATGGACGAGGATTCTAAC
CCGATGCTCAACAAACATCTCCAAACTATTCACAAGCTCGCAATGGATCACGGAGTGGAA
CAGCGTATGGATACCCAGGACAACAACAACGAGAACCACTTGAAACGCACTAACAGCGAG
GGAAACGAATCTCCCAGCAGTAGACTCCCTCCCAGCAAACAGCGACGTCTGGACAACGAC
GAGAACGACCAGCAGACGCAGAATTGCCACGATCCCGCCAGAAAGTGCAGTGAATCACTG
CAGGCGCTTCCGCCGTCACATCGCTCGCGCAAGCAGAAGCACGAGCGAATCCTGAACACA
GATCTGGAGGGTTCGCTTTTTCCACCCACCCAACAGCAGTCGATTTCAACGCCGGATCAA
AATGGAGCCTTGTCCTCGGAGGTCGAAGGCGAGGACGCAAAGGCGCAAGATCCGCTAGAA
CCGGCGACGCCTCAGCCCCCACCGGTAGCGACGCCGGCAGGAACGGGCAAGAAGCGTGGG
CGGCCGAAGCGTATCCAAAACCAGAGCTCGCCGGATGGTGGAGGTGCAGTTACTCCCAAA
CCCGGCACTCCACAGGAGGAGGCCAACAGCGCTGTCAAGTCGCGTCGCGTACAACTGCTG
CGCAAGCGACTGGCCATCGACATGGTGAGCGTGGGCCAAGAGCAGGCGGACATGAAAGCT
AAGGAAAAGGAGTCGTCTGTGGGCGTGCCAAATGCAAGAGTTGAAGACGGGCTTGCCGAA
ACTCTGGAATCTCCAAAGACTCGCGACCATCGCCCCCTGAGAGCCACGCGCCGCACCACG
ACCAGCACAAACACCAACCTGCAGCCCACGCCCAAGTCCACAAGGAAGCGCCAGAGCAAG
GCCAGTGTCCAACAAAGCCAGTTGCCGCCGCCGACGATGGCTCAGTTCGGTAGTTCGGAG
TCCGAGTCCAACAACAACAATAATAGCAGCATATCCTTTGCCCTGAGCGCGCAGATCGAC
CTCACAATGTGCTCGTCCAGTTCGTCGACCTCCTCTGGCGCTGCCGCCAACCAGCAGGTG
ATCGGTGGAAGTGGCAGCAGCTCGATGCTACCTCCGACGACGATTCTGTCTTCTTCGGAT
CCGCTGCCCGATGTCATCTTCCAGCCAAATGACTTCTCCTCTATCATGGCCACCCAGCAG
CTGCGCTCCTCGCGCCCCTCAAGCATCAGCTGTGGCAGTACCGGCGGTAGCCAGCCGGAC
TGTCACGACGAGGACAACTACACCAGCGCGCTGGACAATTCCGGAGACGAAACTGCGTTG
CCTATGCCCCCCACGCGGGGACGTGGTCGTGGCCGGCGATCCAGGGGAGGCCGCGGTAGA
GGATCTTCTTCGGTGGATCGGGCTGTCAGCGTCGGAGGCACAGCCTCCACAACGCCCGCC
ACGCAGCCTAGGGCCCCGCGAATGAGCCGAGGAGCAAGTGCCGTAGCAAAGGCCATCGCC
ATGAGTCGACCACGATGCGTGGGCGGCCTCAAGCACCAGCCCGATCCCGAGAGACTTAAA
GGCTTGTTCAGTCCGTCGCCGCAAGTGTTTGAGGAGGACACGCGTATGAGCGCCGATCTT
AGCAACAGCAATCAGTCGATGGCCAGCCTGATGGAACCCCCGCTAACGCCACAGAAGCAA
CCCGACTTCCTCAACAACGAGGAGTCACAGTCGTCCATGGTGAGCAATGTGAGCATGATG
GACTCCAACCAGGGTCAGTCCGCCGACGCGATTCTGGGTTCTGCTCTCAAACGTCCAAAG
AAGAAGAAGATGGAAACTTGCGTGGCCGAAGACACCGACTACAGCGCCTCCAGCATCGCC
GAGTACGACTGGCCACCGCCTAAAGGTTGCTGCCCATCAAAGAATCGGGACACCTTCATG
ATTCAAGAGCAGGTGGCGCTCTATCTGGGCATTACCAGCTTTAAGCGCAAGTACCCGGAT
CTGCCACGCCGATCGGTCGACATGGAGGAGCGCAATTGGCTGCAGGAGAAGGGGCTGGTT
AGCGAGAAGTTGTGCGACCTGGGCATCACCGCTGTGTGGGCCTCCGACATATTGGACATC
ATGTACGCGGACTTTTTCGACAAGTACGAAGAGTACAAGGAGTACATTCGCAGAAAGCAT
TTGCGCGAGATCGAGGCTAAGCAGAAGGCCCTAGGACTCACAGTCGGGGCCGGACGAGGT
CTGCAAGCTCGCGACCGTGCCATGCTTTCGGCCACCAAGTGGAACGCTTACTTCAACAAG
TCCCGTAAGGATGAGCGCCAGTCCTGCATGGACCTGCAGACCTTCACGATAAATCAGCCG
CAGCCAAGGACCGCGCCCACCTGTACACGCCTAGAGCGCTCCACTTCGGATTTGATACGG
GCAGTGGCAGAAGAGGCGAATATCCCGGCACCGCCTAATCTCCTGCCACCCCGCGACTAT
GACGAGGCCTTCCGCAACTCAGACTACGCTTATCCACTCACGGTGGTACCGGACCAGTTC
TCAATGGCCTACCGCCAGTTTGAGCCCGCCGAGCTCAGAGCCTACCCACTGGACACGGCG
CTGGACAAGCCGCCGACCGATCTGATGGCTCAGCTGCTACAGGCAAAATCCGAAGCTGTA
GGCAGCGATGAAATCAAAACGTCCGCCCCGGCCCCCAAGGATCTGGGGCAAGAGCAGTCC
GCCATCAAATCCGTAACGGTAACGGCGCCCGTCCGACGCAGCAGAAGGTCCACACGCCAG
CAGACGGACAAGGTTCGCACGGCTAGCAGCTCCAGCACGTCCTCCGCTCAATCGGTCTCA
TCCGCGTCCAGCGGCAACGGGAGCAGCAGTGATACGGAAAGCGGCGATGAAAGCGACTTT
AGCAGCACATCGAGCTGCAGCAGTTCGACGGGTGCCTCAAGTGGAGCAGGCAGCGAGGAC
GAGGATGGGAACGAGTGCTCATCGTCGGTCAGGCTGTCCACCTGCGGCGTCTGTCTGCGC
AGTCAGCATCGCAATGCTAGGGACATGCCGGAGGCTTTCATCCGCTGTTACACATGCCGA
AAGCGCGTCCATCCCAGCTGCGTTGATATGCCACCGCGCATGGTGGGTCGCGTGAGGAAC
TACAATTGGCAGTGCGCCGGGTGCAAATGCTGCATCAAGTGCCGTAGCAGCCAACGTCCA
GGAAAGATGCTCTATTGCGAGCAGTGCGACCGAGGCTACCACATCTACTGCTTGGGCCTC
AGGACAGTCCCAGATGGGCGCTGGAGCTGTGAGCGCTGCTGTTTCTGCATGCGATGCGGG
GCCACCAAGCCGGAGGGTCTGCCCCAGGTGGCCGCTCTGTCCCAGGCGTCCGGAGGACCG
TCGGCCAACGGGGATCGCTCAAAGGCAGCGCGCAATAAGCGCTTGAAATGGGTGCACGAG
TACCGCATCGATCATGTGACGAAGATCCGGGAGCACGCCGCCATGTTCTGCGTACCATGT
GCGCGAAACAAGCCCGCCAAGCGGCAGTCGGCGGCGGGTGCGGCCGGTGCGGCAGCAGTT
ACTCCTGTCTTGGAGGCCACTTCCGCGCAAACGGATGACAGTCCGATGCCCAGTCCCGGC
CTGACAACCAACGGAGGACGCGCCCTTTCGCCAACGGCGGCACTGAGTCCAAAGGCAGCT
GTGCCCGTGGCATCGCTGCCGCCCGTTCTTGAAGCGACGACGGTGACCACAAATATTGCG
GGAACGATAGGCAGACGGCAGGCGGGTAATGCTGTCAATATTACAACGATGCAGTGCAGC
AGCAGCAGCAGCAGCAACTTCAGCGGGAACGGAGTCACCGAGGACGCGGCGAATGTCACT
GCAACGGGAACGGCTACGGCGGCCGCAGGAGCACCCGCTGCCACTCCAATAGGCATAGCT
CCGCCGCCCGTTGTTGCC
Protein sequence predicted based on nucleotide sequence
MNDLRQQQMVAATSSSGSESGTAVESAAATSTAGSAGAAGRPQSNCSANSNAKSVAASST SEEEQRVSSTSSPAQRDQQLNADRDREQEPEPQQQQQREEALQHQHNQPGHITSTTASPP PTLPPPTTPCDDAPSTTGASASASSASGEAPSAASAAGAAGGPMAATALEVESEERDGHK IILKLSKHANPNSNANESQPGGDERRVEPLRIQLPCGGAEGGLVAKQQDAFDADASSCSS SCAEDEVATTLGQQLRNTPHIVPKLTIRAANERRVGSVVPKLTIKLPENPAASGSNSNSG SCSAAVSGAQSAMPAKNDAHLSSLSPASASSSSASSSSSSSSSSSLAEMQTVPKLMIKTT LAGSSCISSSEELPQQQQQIPKLTIKTGGGGQEHVHTVIMTHDLNNAQSIPKLTIKTKSI EMIEDEQAAKLEQQLQPLPKLTIKNLCSPKHKVRAVLEEKPPTAASKLAIPKPTPNPTPA PMTNGGESNSSSQEFCGFSDPDADIAAPASDDVRRNSDDMVIDDSLSKEHDPKIFHNLPP MPASNGIASGGSKASKASKSAQSQHNVVDMVDLTSSPSPGSSPTHVPNNFTGRISPKGLL IDCLRMQTAIGCYVPEEADSRVRPAASPNSNILLSQLTAPAKSFTTTTPKSKSSKYPQLT ERLMANGSGTGGGSVIAAESEAVVGPLPAANPAQSIIESIEILDTPDGSPRVAYMDEDSN PMLNKHLQTIHKLAMDHGVEQRMDTQDNNNENHLKRTNSEGNESPSSRLPPSKQRRLDND ENDQQTQNCHDPARKCSESLQALPPSHRSRKQKHERILNTDLEGSLFPPTQQQSISTPDQ NGALSSEVEGEDAKAQDPLEPATPQPPPVATPAGTGKKRGRPKRIQNQSSPDGGGAVTPK PGTPQEEANSAVKSRRVQLLRKRLAIDMVSVGQEQADMKAKEKESSVGVPNARVEDGLAE TLESPKTRDHRPLRATRRTTTSTNTNLQPTPKSTRKRQSKASVQQSQLPPPTMAQFGSSE SESNNNNNSSISFALSAQIDLTMCSSSSSTSSGAAANQQVIGGSGSSSMLPPTTILSSSD PLPDVIFQPNDFSSIMATQQLRSSRPSSISCGSTGGSQPDCHDEDNYTSALDNSGDETAL PMPPTRGRGRGRRSRGGRGRGSSSVDRAVSVGGTASTTPATQPRAPRMSRGASAVAKAIA MSRPRCVGGLKHQPDPERLKGLFSPSPQVFEEDTRMSADLSNSNQSMASLMEPPLTPQKQ PDFLNNEESQSSMVSNVSMMDSNQGQSADAILGSALKRPKKKKMETCVAEDTDYSASSIA EYDWPPPKGCCPSKNRDTFMIQEQVALYLGITSFKRKYPDLPRRSVDMEERNWLQEKGLV SEKLCDLGITAVWASDILDIMYADFFDKYEEYKEYIRRKHLREIEAKQKALGLTVGAGRG LQARDRAMLSATKWNAYFNKSRKDERQSCMDLQTFTINQPQPRTAPTCTRLERSTSDLIR AVAEEANIPAPPNLLPPRDYDEAFRNSDYAYPLTVVPDQFSMAYRQFEPAELRAYPLDTA LDKPPTDLMAQLLQAKSEAVGSDEIKTSAPAPKDLGQEQSAIKSVTVTAPVRRSRRSTRQ QTDKVRTASSSSTSSAQSVSSASSGNGSSSDTESGDESDFSSTSSCSSSTGASSGAGSED EDGNECSSSVRLSTCGVCLRSQHRNARDMPEAFIRCYTCRKRVHPSCVDMPPRMVGRVRN YNWQCAGCKCCIKCRSSQRPGKMLYCEQCDRGYHIYCLGLRTVPDGRWSCERCCFCMRCG ATKPEGLPQVAALSQASGGPSANGDRSKAARNKRLKWVHEYRIDHVTKIREHAAMFCVPC ARNKPAKRQSAAGAAGAAAVTPVLEATSAQTDDSPMPSPGLTTNGGRALSPTAALSPKAA VPVASLPPVLEATTVTTNIAGTIGRRQAGNAVNITTMQCSSSSSSNFSGNGVTEDAANVT ATGTATAAAGAPAATPIGIAPPPVVA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
