Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
1280
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00763808
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3222nts
Open reading frame: 1 to 3222
ATGGCCTGGCGATTCAAGGCTTCCAAGTACAAAAATGCCGCACCCATTGTGCCCAAGGCG
GAGGCATGCGTCCGCGAGATCTGCGTGGGCAGCTACCAGACGTACGGTAACAACATCGCC
GCCTCCGGCGCCTTCATGGCCTTCAACTGGGAGCACACCGGGTCCAGCGTCGCTGTCCTC
CCGCTGGACGACTGCGGCCGGAAGAGCAAGACCATGCCGCTGCTCCATGGACACACGGAC
ACAGTGACGGACCTTAAGTTCTCCCCGTTCCACGACGGGCTGCTGGCCACCGCCTCGCAG
GATTGCCTGGTCAAGATCTGGCACATACCGGAAAAAGGTCTGGAGCAGTCGCTTTCCGAT
CCGGAGGCAATCTTCTCACACAAGCAGAGACGCGTAGAGACAGTGGGCTTCCATCCAACA
GCAGATGGTTTGATGTACTCCACAGCCGCCGGCTGCGTGGCTCTCTTCGATCTCAGCACT
CAAAAGGAGATATTCTCGAACAATGAGCACCCCGAGGTGATACAGTCGGCCAGTTGGCGC
GAGGACGGTTCTGTTTTGGCCACCAGTTGCAAGGATAAGAATGTGCGAATCTTCGATCCC
CGTGCCGCTGGTTCACCCATCCAGCTGACTGCTGAATCGCATCAGAGCATCAAGGATTCG
CGGGTGGTGTGGTTAGGCAATCAGCATCGCATCCTGACCACTGGCTTCGATGCGGCCCGC
CTGCGACAGGTGATCATCCGTGATGTGCGCAACTTCAACACTCCAGAGAAAACTCTCGAA
CTGGACTGCTCGACGGGCATATTGATGCCCCTCTTCGATCCCGACACCAATATGCTTTTC
CTAGCCGGAAAGGGTGACACAACCATTAACTATTTGGAGATTACGGACAAGGATCCGTAT
TTGATTGAGGGCTTGCGTCATACTGGCGAGCAGACGAAGGGAGCTTGTCTCGTGCCCAAG
CGGGCACTTAAAGTCATGGAAGCAGAGGTTAACCGGGTGTTGCAATTGACCTCCAACATG
GTCATACCCATCATGTACCAGGTGCCCAGAAAGACATACCGTGATTTCCACGCCGATCTA
TATCCGGAGACCACTGGCTACAAGACAGAACTGGTGGCCGGCGAGTGGCTAAATGGCAGC
AACCAGGCGGTGCCCAAGATGAGCCTTGATCCCGCCAAGCGTGAGCATGGCGATGAGCCA
ATTATTCCACGTTTGGGTCCCAAGCCCTTCTCCAGCACCACAGGTGACGTTTCGTTTGAC
AAGGTGTTCGCCGTTCCCTTGGCACCGGGATCCCACGAGAATATCTCGAACGTGGGTCAG
GACAGCGGAGTGGAGATGACACCAGCTCAGGGAGCCAAACCGGATCTCATTGTGGAAATC
GAAATTAAAAAGAAACACGAAAGGGAACCTGCTGTGTCCGGAAATGGAGTCCAGAAGTCA
CTGACCACTTCGGAGCGCCGTAAGATATTCGAGCAGAACTCTGAGTCATCGGAGAACTCG
ACGGAGGGGGAAGATCGCACGGATGCGGATCTGCGAAGAAACTGCACCTCCAGGAGCAGC
TTTGCCGAGCGACGGCGCATATACGAGAATCGTTCCAAGAGTCAGGTGGACGAGAAGCCT
CAGTCGCCAGTGCCATTACGTCGCGAGCACTCCAAAGTGGAGCCGCTGAAGCCCAACCAA
CAGCAGCAGCAGCAGGGCAATGTGATCGACACCAAGCGCATCTCTGTTCCAGAGGGCAAA
TTAATGGAGGAGCATCGCCGCGGAAATGGCGCTGGACTCAAGAAGTCCGCCACTGAGGCG
GCTTTTAGCGCTGCCAGCACCAAGCGCACTTCCACCGTCTTCGGCAAGGTGTCCAAGTTC
AGGCACTTGAAGGGCACACCCGGTCACAAGTCCACGCACATCGAGAACTTGCGCAATCTA
AGTCGCCAGATTCCGGGCGAATGCAATGGCTTCCACGCTAACCAGGAGCGAGTTGCCGTG
CCACTTTCGGGGCCCGGCGGCAAGATAGCCATCTTTGAGTTGAGCCGCCCTGGACGGTTG
CCCGATGGTGTGATTCCGTCACTTGTGAACGGCAGCAACATCATGGACTTCCAGTGGGAT
CCGTTCGATGCCCAGCGATTGGCTGTGGCATGCGACGATGGCATTGTAAAGATCTGGCAT
ATTGAAGCCGGTGGATTGAGTGAGCCGACAAACACACCTGCCGGCGAGCTAACCGCTCAT
CTGGACAAGATTTACTTCATACGCTTCCACCCGCTGGCTGCTGATGTTCTGTTAACCGCC
AGCTACGATATGACCATCAAGCTGTGGGATTTGCGCACCATGACCGAGAAATGCTCGCTT
TCGGGGCATACAGATCAAATCTTTGACTTCGCCTGGAGTCCCTGCGGCCGATTGGGCGCC
ACCGTGTGCAAGGATGGCAAGATTAGGGTGTACAACCCAAGGAAATCGGAGACACCCATA
CGGGAGGGCAATGGACCGGTCGGTACTCGTGGAGCTCGGATCACCTGGGCGTTGGAGGGA
CATTACATCGTCTGCACTGGTTTTGACAAGGTTTCGGAGCGACAGATCAGCGTGTACAAT
GCACAAAAGTTGTCCGCCCCATTGAACACTGCCAGTTTGGATGTCTCACCCTCCATACTG
ATTCCATTCTATGACGAAGATAGCTCCACACTGTTTGTTACGGGCAAGGGTGACTCCACA
ATCTACTGTTACGAAATCACCGACGAGGAGCCGTACATCTGCCCACTGTCGCACCATCGT
TGCACCTCGCTGCACCAGGGCCTCAGTTTCCTCACCAAGAACCACTGCGACGTGGCCAGC
GTGGAGTTCTCAAAGGCCTATCGGCTAACCAATACCACAATTGAACCCCTGAGTTTCACA
GTGCCCCGCATCAAGAGCGAGCTGTTCCAAGACGATCTGTTCCCACCCACACGCATCACC
TGGAGCGCCACACTGAGCTCCGAGGATTGGTTCGCTAGCAATGATAAGGCGGCACCCAAA
GTCAGCCTCAAGCCCGAGGGCATGGAAACTTTATCTTCCATACAGCAAGTGCCAGCGCAG
CCCGTCAAGAAGCCAGATCATCCCCAGTTCGGTGGCCAGAAGTCCGAGTACGAGATCAAC
AAACAGCAGGAGATCCAGAAATCGGTTAGCGCGCGCATGGAATTCACCACCAAGCTGGAG
CAGGACGACATGGAGGGTGTGGATGAGAACGAGTGGCAGGAG
Protein sequence predicted based on nucleotide sequence
MAWRFKASKYKNAAPIVPKAEACVREICVGSYQTYGNNIAASGAFMAFNWEHTGSSVAVL PLDDCGRKSKTMPLLHGHTDTVTDLKFSPFHDGLLATASQDCLVKIWHIPEKGLEQSLSD PEAIFSHKQRRVETVGFHPTADGLMYSTAAGCVALFDLSTQKEIFSNNEHPEVIQSASWR EDGSVLATSCKDKNVRIFDPRAAGSPIQLTAESHQSIKDSRVVWLGNQHRILTTGFDAAR LRQVIIRDVRNFNTPEKTLELDCSTGILMPLFDPDTNMLFLAGKGDTTINYLEITDKDPY LIEGLRHTGEQTKGACLVPKRALKVMEAEVNRVLQLTSNMVIPIMYQVPRKTYRDFHADL YPETTGYKTELVAGEWLNGSNQAVPKMSLDPAKREHGDEPIIPRLGPKPFSSTTGDVSFD KVFAVPLAPGSHENISNVGQDSGVEMTPAQGAKPDLIVEIEIKKKHEREPAVSGNGVQKS LTTSERRKIFEQNSESSENSTEGEDRTDADLRRNCTSRSSFAERRRIYENRSKSQVDEKP QSPVPLRREHSKVEPLKPNQQQQQQGNVIDTKRISVPEGKLMEEHRRGNGAGLKKSATEA AFSAASTKRTSTVFGKVSKFRHLKGTPGHKSTHIENLRNLSRQIPGECNGFHANQERVAV PLSGPGGKIAIFELSRPGRLPDGVIPSLVNGSNIMDFQWDPFDAQRLAVACDDGIVKIWH IEAGGLSEPTNTPAGELTAHLDKIYFIRFHPLAADVLLTASYDMTIKLWDLRTMTEKCSL SGHTDQIFDFAWSPCGRLGATVCKDGKIRVYNPRKSETPIREGNGPVGTRGARITWALEG HYIVCTGFDKVSERQISVYNAQKLSAPLNTASLDVSPSILIPFYDEDSSTLFVTGKGDST IYCYEITDEEPYICPLSHHRCTSLHQGLSFLTKNHCDVASVEFSKAYRLTNTTIEPLSFT VPRIKSELFQDDLFPPTRITWSATLSSEDWFASNDKAAPKVSLKPEGMETLSSIQQVPAQ PVKKPDHPQFGGQKSEYEINKQQEIQKSVSARMEFTTKLEQDDMEGVDENEWQE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
