Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
1291
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00763814
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4281nts
Open reading frame: 1 to 4281
ATGCGAGCATTTTGCACCGTCAGCGCGCCTTTGGAAGTATGCGCGTCCTCAGCCGAGCAA
CTATCACCAGGCTCGCGATTCCTGGCCCTGAGACTTCTGGGGCAGCAGCAGCCGAAAACC
TTGTACTTCCTAGTCGATGCCAAGAGTCGAGTTCGAGAGGTTTACACGCAGACATGTCTG
CACTTTGCCACGCAAGGGATGCTGGACACCGAACTCTTCGGTCTGGCTGTGCTCATAGAT
GGCGAGTACATGTTCGCAGATCCCGAGAGCAAGCTCTCAAAATATGGCCCAAAGAGCTGG
CGATCATCGCACACCCACGGGCTGGATGCCAACGGACGACCGCTCCTGGAGCTACACTTT
CGCGTGCAGTTCTACATCGAGAGCCCGTTCATGCTGAAAGATGAGACGTCCCGCCATAAT
TACTACCTGCAGCTGCGCCACAACATCTTGCAGCGCGATTTGCCGAGAGAACAGGCGGAG
CAGGCACTGGTCTTCCTTGCTGGACTTGCTCTTCAGGCGGACCTGGGTGATGCTCCTCCG
GGCACGAGTAATTCCAAGGATGATTCAGGAGAGGAGACATCGGCATCTCCTTCTAACGGA
GGAAGAGGTCTAAGTGCCACCACTACATTACCCAAGATCAGCAAACGTGCTAATGAGAGG
ATGCTTAGACTCTCGACATATGTGGCGTCCACATCAAAGAGAGAAACAATACCATTACCA
CCCAGCCTGCCACCGAATGGAGCAGATTACTACCGGATCGAGGATTACCTACCCAGTGGA
CTGCACACTCCCTGGGCGAGAAGTGCAATGAGAGCCTGTCATCGGGAGCATTTGGGCATG
GCCACGGCAGAGGCGGAGCTGCTATATATTCAACAGGCATGCAGTCTTCATGAAACCATC
AATGCCCATACCTACAGGATGCGATTGGCCAAAAGCGAGCAGGGATCTGGTAGTGCCTGG
TTTGTGGTCTATGCCAAGGGAATCAAGATTCTGGGAGGAGAGTCTACCAACAGCTCTTCC
AATCCCGAAACCACAACCTTTCTGTGGCCGAATATCACGAAGCTCTCCTTCGAGCGGAAA
AAGTTTGAAATACGATCTGGAGAGAGCCGAATCACCCTGTACGCCGCATCCGATGAGAAA
AATAAACTCCTATTGACCCTCTGCAAGGATACACATCAGTGGAGCATGAAACTGGCTGCC
AGGCTAAAGGAGGTATCCAAACGCGAGGAGGAGGAGGCTGCCGAATCACAAAGGCTACAT
GCCAGCTACGCTTGTTCCAGAAGTCTGCTCTTGCCCTACAAAAGTAAAAACGAGCAGCGA
ATATCGGTTATCTCGAGTACCAGCTCCAACACAACCTCTGGGATAGTAAGTGATCGAGTG
CACTCCGAAGACGAGCTGGAGATCATGATCAATACACCACCAGCACCCTTAGCTGCTCCG
TCAACGGAAAGTTTGGCCTTGGCTCATCTTTTGGACAGACCCAGTGTGAGCAGACAAACT
TCGAGCGTGGGTCAGATGTCTCTGAAGGATTTGGAAGAACAACTGGCTGCCTTGAGTGTG
AGGCCACAAGACGCGAGTTCTAACGGGGCCACGATCGTCACGAACTCCTCTGTTCAGAGA
AATTCCATGGGAACAACTGCCAATGATTCATCTACCGCCACTGATTCACCCAGTTCGCAG
CATAACATCGGCTCTCAGTGCTCATCCACTTGTAGCACTGTGGTGGTAACATCTCCTGTA
AATGGAGCAGGCGCTTCCTCTTCAGGTGCTCCCATACCTGTGCACTCCACTTCATCTAGT
TTGGAGCTTGGCTTTAGTCACACCGCTCAAAACTCGGCCTTGAGCGAAACGAGTCCCGAC
GATTTTCTCTCCACATCGGCAAGAGAAGAAACGGAGAGTGTATCGGGCGCATCTGGGGTT
TATACTTTGGCGCACGGAGCTCCACCCACGGAAACCTCAGGCGTTTACACCATGCACAGC
AGTGAGCTAACTGGACAATCATCGGAGATCGCCGAGTCGGAAAAGAGTTCGCACTACGGG
ATGTTCCAGCCCCAAAAACTAGAAGAAACCCACGTGCAACATTCTGATTCCGTAGATGGA
AAAAAGAAAGAAGACTTCAGACCGCGATCGGATTCTAACGTCAGCACAGGGAGTTCCTTT
AGAGGCGATGGCAGCGATCCAACAGACAACAAACATTCCTTGCTCTCCGCAGAAGAGTTA
ACCAATTTGATTGTGGGCAGGGGGACCTACCCCAGTCGGAAAACAGTATCTAGTAGCCTT
CACTCGGACTGTGATTACGTGACGTTACCACTAGGAGATCAAGGAGAAGAGGAGGTAGAC
CAACCACCAGCACCACCACCACCCTACAGTGCTAGGCATGAGAAAACTGGTCTCTGTGGC
CCACCTATCGCCAAACCTATCCCTAAACCTATAGCTGTGGTAGCTCCCAAACCAGATTCA
CCGCCATGTAGTCCACCGGTACCGCCAGCACCAATTCCAGCCCCACCGCCGGCTATTCGA
CGAAGAGATCCACCGCCATATTCCATCTCTAGCAAGCCTAGACCCACATCCCTGATATCT
GTCAGTTCGTCGGCACATCCTGCGCCCAGTGCTGCCGGCTCGATGAGTTCTTTAAAGTCC
GAGGAGGTTACAGCCAGATTTATCACCACCAGGCCACAGATTAGTATTCTGAAAGCCCAC
ACCAGCCTGATTCCCGATGGGGCCAAGCCAAGTTACGCGGCACCGCACCATTGTTCATCA
GTTGCCTCCTCCAATGGATCGGTTTGCAGCCATCAGTTGAGTCAGCAATCGCTGCATAAC
TCCAACTATGCCGGCGGATCCCAGGCTTCACTGCACCATCATCACGTGCCGTCACACCAC
CGACATTCTGGATCGGCAGCCATTGGAATAGTTCCTTACGGTCTGCACAAGAGCACGGCA
TCACTGCATCATCAGCAGTCATGTGTACTACTACCGGTTATTAAACCTCGGCAGTTCCTG
GCACCACCACCGCCCAGTTTGCCGCGGCAGCCTCCACCACCGCCCCCACCGAATCACCCC
CATTTGGCGAGTCACCTTTACGAGAGGGAAATGGCTAGAAAACAATTGGAGCTCTACCAG
CAGCAGCTTTACAGCGATGTGGATTACGTAATTTACCCCATCCAGGATCCAGCTGTAAGC
CAGCAAGAGTATCTGGATGCCAAACAGGGATCCTTACTGGCGGCAATGGCCCAAGCAGCA
CCACCCCCACCGCACCACCCATATCTGGCCATGCAGGTGTCGCCGGCGATTTACAGAAGC
ACCCCCTACCTACCACTGACTCTGTCGACCCACTCACGATATGCATCCACCCAAAATCTA
TCGGATACATATGTGCAGCTTCCAGGACCCGGATACTCACCCCTGTACTCTCCTTCGATG
GCCAGCCTATGCTCCTCGTATGAGCCTCCACCGCCTCCTCCCCTTCACCCGGCTGCTCTA
GCAGCAGCTGCTGCGGCGGGGGCTGGCTCCTCGTCTTCCTCGATGTTTGCGCGATCGCGA
TCAGATGACAATATTCTGAACTCACTAGATTTGCTGCCCAAGGGAAAACGACTGCCACCG
CCGCCACCACCACCGTATGTAAACAGACGGCTAAAGAAACCACCCATGCCGGCGCCCAGT
GAAAAGCCACCTCCAATACCCTCTAAGCCAATTCCTAGCAGGATGAGCCCGATTCCACCA
CGAAAACCCCCAACGTTGAATCCGCATCATGCGAACTCACCGCTAACAAAGACCTCCAGC
GGCGCCCAGTGGGCAGGTGAGCGTCCGAGACCTGACTTGGGTCTTGGTCTGGGATTGAAC
CGGGGAAACAACAGCATTTTGGCCCAGCTGCAGGCTTCAATGGTAGCCCAGTCTCATGCC
CAGGCACAGGCCCAAGCGCTAGACATTGCGCTTCTTCGGGAGAAGAGCAAGCATCTAGAT
CTGCCGCTGATCTCGGCGCTCTGCAACGATCGCTCCTTGCTGAAACAGACTAAGGTTGTT
ATAAATCCGAAGACTGGCCAGGAGATGCCCACTTCCAGTGCGCAACCTTCAGGTGCCACC
ACAAATGGAGTTGCCAACTCCTCTGCAGGAGCAGGAACCCTAAGCAAGGCCAGAAAAGGA
TCTACCGTAAGCCACCGTCATCCGCAGGACAAACTGCCGCCTTTACCTGTCCAACAATTG
GCGGAGGCCAACAACTACGTGATCGATCCCGCTGTCATGATGAAGCAACAGCAGCAGCAG
CAGCAGCACAACAAGACCAGC
Protein sequence predicted based on nucleotide sequence
MRAFCTVSAPLEVCASSAEQLSPGSRFLALRLLGQQQPKTLYFLVDAKSRVREVYTQTCL HFATQGMLDTELFGLAVLIDGEYMFADPESKLSKYGPKSWRSSHTHGLDANGRPLLELHF RVQFYIESPFMLKDETSRHNYYLQLRHNILQRDLPREQAEQALVFLAGLALQADLGDAPP GTSNSKDDSGEETSASPSNGGRGLSATTTLPKISKRANERMLRLSTYVASTSKRETIPLP PSLPPNGADYYRIEDYLPSGLHTPWARSAMRACHREHLGMATAEAELLYIQQACSLHETI NAHTYRMRLAKSEQGSGSAWFVVYAKGIKILGGESTNSSSNPETTTFLWPNITKLSFERK KFEIRSGESRITLYAASDEKNKLLLTLCKDTHQWSMKLAARLKEVSKREEEEAAESQRLH ASYACSRSLLLPYKSKNEQRISVISSTSSNTTSGIVSDRVHSEDELEIMINTPPAPLAAP STESLALAHLLDRPSVSRQTSSVGQMSLKDLEEQLAALSVRPQDASSNGATIVTNSSVQR NSMGTTANDSSTATDSPSSQHNIGSQCSSTCSTVVVTSPVNGAGASSSGAPIPVHSTSSS LELGFSHTAQNSALSETSPDDFLSTSAREETESVSGASGVYTLAHGAPPTETSGVYTMHS SELTGQSSEIAESEKSSHYGMFQPQKLEETHVQHSDSVDGKKKEDFRPRSDSNVSTGSSF RGDGSDPTDNKHSLLSAEELTNLIVGRGTYPSRKTVSSSLHSDCDYVTLPLGDQGEEEVD QPPAPPPPYSARHEKTGLCGPPIAKPIPKPIAVVAPKPDSPPCSPPVPPAPIPAPPPAIR RRDPPPYSISSKPRPTSLISVSSSAHPAPSAAGSMSSLKSEEVTARFITTRPQISILKAH TSLIPDGAKPSYAAPHHCSSVASSNGSVCSHQLSQQSLHNSNYAGGSQASLHHHHVPSHH RHSGSAAIGIVPYGLHKSTASLHHQQSCVLLPVIKPRQFLAPPPPSLPRQPPPPPPPNHP HLASHLYEREMARKQLELYQQQLYSDVDYVIYPIQDPAVSQQEYLDAKQGSLLAAMAQAA PPPPHHPYLAMQVSPAIYRSTPYLPLTLSTHSRYASTQNLSDTYVQLPGPGYSPLYSPSM ASLCSSYEPPPPPPLHPAALAAAAAAGAGSSSSSMFARSRSDDNILNSLDLLPKGKRLPP PPPPPYVNRRLKKPPMPAPSEKPPPIPSKPIPSRMSPIPPRKPPTLNPHHANSPLTKTSS GAQWAGERPRPDLGLGLGLNRGNNSILAQLQASMVAQSHAQAQAQALDIALLREKSKHLD LPLISALCNDRSLLKQTKVVINPKTGQEMPTSSAQPSGATTNGVANSSAGAGTLSKARKG STVSHRHPQDKLPPLPVQQLAEANNYVIDPAVMMKQQQQQQQHNKTS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
