Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
1283
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00763821
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4200nts
Open reading frame: 1 to 4200
ATGGCCACGAACTGCGACTTCATCGAGCAGCCAGTGAAACAGCAGCCGTTGGCCAATGCA
CCAGTCATGGGCGTCCAGGAGGATAAGGCACAGGAGCCCAAGGAGTTGCCGGATGAGCCG
GCGAAGCAGCCAGACGGCCAACCATACCAGCATTTCGCTGGCGAGGATAGCTTCGAATGC
GCCTTCGAGTCGACCATGGACGACCTGGAGCAGGGTGTCCTGGATGGTTACGAGTCGGAC
TCGGAGGCGAGTGCCAACGACCAGATCACTGATTCCGCCTCCTCTGGCAGCCTGGGCCAA
TTGGCCCAGCAGCAGCAGCGCGAGGAGCGATTGAGGGGCTTGCCGGGTCCACTTCATGGC
CAGCATATCGATTCCGAGATGCTCAAGTGCATGCCGCGATCGAACTCCTTCGACTTTCAC
AGCAGCAGTGGCAGCAGCTCGGATGACGAGGATGATCCGGATGATGACGACGACGATCAA
GTTCACCACAGCCTGGGGCTCAGCCTCAGCGGGAAGTTTGTCAAGGTTGATCTCTATTCG
GGTCACCAGAGACCCGGCTATCTGTTACCTCCCTACATCGAACGACGCCGCTTGTCGCAG
TGCAAGGAGGAGGATGACGAGGATGAGGGCGACAGGACGCAGCTGGGCGGCGGCTCATCA
TCCACGGGAGAGTCAACCAAATCCGAAAAAGCTTCGAAGGCGCCACCAAGGCCACCCACG
CCACAGGATGAGGCCTCCAAGGAGAAGTTTAAGGATCTGGAGCGTCTGCGGCAGCAGTTT
ATGCACAAGATCGATAGGATCAATACGCGAAATATCGAGGAGAGTGAGGCACCGCCGCCA
CCACCTCCTCCGCCGCCTCCCATACCAGCCACGATTCTACCAAAGGCGCTAAAGGCGCTA
CTGCACACCCAGGAACCAGTGGCGCCTCCTTCCCGTCCATCTCCAGTTGGCCCACCAAGT
CCTGCACCCACGTCCAGCGTTATTGTGGCAAAGGAAACACCGGTTCCTGTAACACCGGAG
CCCGTTTCCCCGCTGCCGCTGCCCACGGAGCCGCCGCTGATTATCAAGGGCAAATTTAAG
GTGACACTCGCCCAGGAGACACCCGAACTGCGAGTCGAGGCCCAGAAGCTGAGCAAACTG
AATGCCAGCTCGAATGCCCACACCATCAGCTTTCCCTCCAGCTTCGGAGGCTACTCCTCG
GTGCAGGGACTGTTCTCCCAGCGATATGGCTCAAATCGGTTTCCCACCGCCGCTCCCCAC
ATGTCCAAGCAGTTCTTCGAGCCATCGCTGGTGGAGATTCGCACGCCGGCCCAGAGCAAC
GTGGCGCTGAATACAACTGGGCTGGCCAATAGCAATCACCAAAGCCTCACTTGTGATACA
AATAGTTTAGCCAACAAGCCGCGCACGCCCAGCGAACGGGAGCTGGACGAGATCTGGATA
CGGCGGCAGGACGGTGGATTGAGTGGCACTGCTGCACCAGGCGCACCGGGTGCTCCACTT
CCTCTGCTCCAGCAGCAGCAGCAGCAGCAGAGACCCGTCTCCCTGGGTGGGGCGTCTACC
ATGCCCCGTTTGCTGAGGAGCGAGAGCAGTGCCTCCACCACAAGTCCATCCCGCCAGCTC
ACCAACTCGGGATCCGTCTCCGTTTCCGTGTCCGATGATGAGCAGGATGGCAGCCTCGGA
GGAGCCGGCCGGATGCCAGGATCACGACCCTCCAGCGCTCCCGCAGAGCCAAATGCCAAG
GCGGCCAAGAAGGCCTACAAAAAGATGGAAAAGGAGCAGCGTCGCCAGGAGAAGGAGCAG
TCGCGACGGGAAAAGGAGGCCCGCAAGCTGGAGCGCGAGACGGCGAGAAGGAACGAACGC
GAGGCGGCCAAGCTGGAGAAGATCAACAGGCACAGCGAGAAGATCTCACGCAGCACGGAG
CGAATGGCCATGGCCGGTGTGGGATCGCGGTCCGGTTCGCTGGAGCGGAGGCGAAGCGGC
GAGGACTCGCCCGTGCTCAATCAGTCGACGGTGCACGGTATAGCGTCACCCAACCGAAGG
CCAACGATATTCGACGTTTTCCGACCGCGTGCGAAATCCGATGCGAAGCGGCAGAAGGAG
AAGCATCTGCTGGATCCCTCGTCCGCCGACAGCAGCGCCACCAGCAGCACCTACTCCGTT
TCCGGCGGCACAGGCCCGGCCACGAGCTCGGCAGCAGGTGGAGCAGGAGGAGCGGCGGCA
GCAGCAGGACAGGGCGCAGCGGGAGGAGCCGGTGGCCTTATGAACTCCATGAAGGTGGCC
ATGCAGAACTTTAGCCATCGCCAGCATCCCGCCGTGACGATAACGAGCGCCGACGGCACG
CAATCAACGGCAAAGAGCAAGTACAAAGACGGCAGTGCCCATCCGCATCAAGGCAGCGAC
GCGCAGTATTACCACACGGTGACGGCGGTGCGTCCAAACTCTTCCCAACGGTCGCCGATG
ACCAAGGTCATGGATCTGTTCCGGCATCGATCCAGCTCGGTTGTCAGCGAAGCCGACAAA
CGCAAAGCGCGCGCGGCGGCGCATCAGCAACAGTTGGCTGTGCAAAGTGCTCACATGCGT
CGTGCCTCCGCGGATTTGGAGAAACGTCGTGCATCAGTTGGTGCCGCAGGTCGAGGACTG
CGAGGGGATGGTACTTTGGATCCACACCATGCAGCCATCCTCTTCAGAGACTCACGAGGG
TTGCCTGTCGCTGATCCGTTCCTAGAGAAAGTAAATCTATCAGATCTGGAAGAGGACGAC
TCACAGATCTTCGTGAAGTTCTTTCGTTTTCACAAGTGCTATGATCTGATACCCACCTCC
GCCAAGTTGGTTGTCTTCGACACCCAGCTTCTTGTAAAGAAGGCCTTCTACGCCCTCGTC
TACAACGGTGTGCGAGCGGCACCGCTCTGGGATTCGGAGAAGCAACAGTTCGTGGGCATG
CTAACCATCACGGACTTTATCAAGATCCTGCAAATGTATTACAAATCGCCAAATGCGTCC
ATGGAGCAGCTGGAAGAGCACAAACTGGACACGTGGCGGAGCGTGCTGCACAACCAGGTG
ATGCCGTTGGTCAGCATCGGACCGGATGCGTCCCTCTACGATGCCATCAAAATTCTCATC
CACAGCCGCATACATCGCCTGCCCGTCATCGATCCGGCGACCGGCAATGTCCTCTACATC
CTGACACATAAACGCATACTTAGGTTCCTTTTCCTATACATTAATGAATTACCAAAGCCC
GCGTACATGCAAAAAAGTTTGCGCGAACTGAAGATTGGCACCTATAACAACATCGAGACC
GCCGACGAGACGACGAGCATCATCACGGCGCTCAAGAAATTTGTGGAGCGACGAGTCTCA
GCCCTGCCACTAGTGGATTCCGATGGTCGCCTCGTTGACATTTACGCAAAGTTTGATGTG
ATTAATCTCGCCGCCGAGAAAACCTACAACGATCTCGATGTTTCGCTGCGCAAAGCCAAC
GAGCACCGGAACGAGTGGTTCGAGGGCGTGCAGAAGTGCAATCTGGACGAATCGCTCTAC
ACGATCATGGAACGAATCGTCCGCGCCGAAGTACATCGACTGGTGGTGGTCGACGAGAAT
CGCAAAGTGATCGGCATTATCTCGCTGTCCGATATACTGCTCTACCTCGTCCTGCGACCA
AGCGGTGAAGGCGTCGGTGGCTCGGAGAGCTCATTGCGTGCGTCCGATCCCGTTCTGCTG
CGCAAAGTGGCTGAGGTTGAAATACCAGCGACAGCCGCAGCGGCGACGACAACAACCCCG
CCTCGCAGTCCATCGGCCGGATCCGGCAATCGCAGCCTGATCGAGGACATACCCGAAGAG
GAGACGGCGCCGGCGAGGAGCGACGATGCCGACAGTGATAACAATAAGTCCGCCAGTGAG
GATAAAGCCAACAATAACCAGCACGACCAGACGACGACGGCTGCGACAGCTAATGGTGAT
AGCAACAACAGCCCCGTAGAAGTGTCCTTTGCCGATGAGGCGCAGGAAGAAGAAGCTGCC
GACCAGGTCGAGCGCAGCAATTGTGATGATGATGACCAGCCAGCGTTAGCGGAGATTGAG
CGCAAGAATGCATCGATGGACGACGACGAGGACGATGGGATGAGCAGCGCCGTGTCCGCT
GCCTCCGCTTTGGGCCAGTCACTGACGCCCGCGGCGCAAGAAATGGCGTTGGTTAGTGAA
Protein sequence predicted based on nucleotide sequence
MATNCDFIEQPVKQQPLANAPVMGVQEDKAQEPKELPDEPAKQPDGQPYQHFAGEDSFEC AFESTMDDLEQGVLDGYESDSEASANDQITDSASSGSLGQLAQQQQREERLRGLPGPLHG QHIDSEMLKCMPRSNSFDFHSSSGSSSDDEDDPDDDDDDQVHHSLGLSLSGKFVKVDLYS GHQRPGYLLPPYIERRRLSQCKEEDDEDEGDRTQLGGGSSSTGESTKSEKASKAPPRPPT PQDEASKEKFKDLERLRQQFMHKIDRINTRNIEESEAPPPPPPPPPPIPATILPKALKAL LHTQEPVAPPSRPSPVGPPSPAPTSSVIVAKETPVPVTPEPVSPLPLPTEPPLIIKGKFK VTLAQETPELRVEAQKLSKLNASSNAHTISFPSSFGGYSSVQGLFSQRYGSNRFPTAAPH MSKQFFEPSLVEIRTPAQSNVALNTTGLANSNHQSLTCDTNSLANKPRTPSERELDEIWI RRQDGGLSGTAAPGAPGAPLPLLQQQQQQQRPVSLGGASTMPRLLRSESSASTTSPSRQL TNSGSVSVSVSDDEQDGSLGGAGRMPGSRPSSAPAEPNAKAAKKAYKKMEKEQRRQEKEQ SRREKEARKLERETARRNEREAAKLEKINRHSEKISRSTERMAMAGVGSRSGSLERRRSG EDSPVLNQSTVHGIASPNRRPTIFDVFRPRAKSDAKRQKEKHLLDPSSADSSATSSTYSV SGGTGPATSSAAGGAGGAAAAAGQGAAGGAGGLMNSMKVAMQNFSHRQHPAVTITSADGT QSTAKSKYKDGSAHPHQGSDAQYYHTVTAVRPNSSQRSPMTKVMDLFRHRSSSVVSEADK RKARAAAHQQQLAVQSAHMRRASADLEKRRASVGAAGRGLRGDGTLDPHHAAILFRDSRG LPVADPFLEKVNLSDLEEDDSQIFVKFFRFHKCYDLIPTSAKLVVFDTQLLVKKAFYALV YNGVRAAPLWDSEKQQFVGMLTITDFIKILQMYYKSPNASMEQLEEHKLDTWRSVLHNQV MPLVSIGPDASLYDAIKILIHSRIHRLPVIDPATGNVLYILTHKRILRFLFLYINELPKP AYMQKSLRELKIGTYNNIETADETTSIITALKKFVERRVSALPLVDSDGRLVDIYAKFDV INLAAEKTYNDLDVSLRKANEHRNEWFEGVQKCNLDESLYTIMERIVRAEVHRLVVVDEN RKVIGIISLSDILLYLVLRPSGEGVGGSESSLRASDPVLLRKVAEVEIPATAAAATTTTP PRSPSAGSGNRSLIEDIPEEETAPARSDDADSDNNKSASEDKANNNQHDQTTTAATANGD SNNSPVEVSFADEAQEEEAADQVERSNCDDDDQPALAEIERKNASMDDDEDDGMSSAVSA ASALGQSLTPAAQEMALVSE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
