Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
1285
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00764160
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4638nts
Open reading frame: 1 to 4638
ATGCCTTTGGCTATGAGCACCATGGGCAAGGAGACGGAGTCGCATAGCATACCCATCAGC
GAGGGCCAGGATGCGGAGCATATCCTGTACCGTCTGAAGCGGGGTTCCAAGCTGAGTGTT
CATCCGGATGCCTCGTTGCTGGGCAGGAAGATCGTATTGTACACCAACTATCCCGCCGAG
GGACAGAAGTTTGTGCGTACGGAGTATCGCGTGCTGGGATGGCAACTCAGCAATGGCAAG
CAGATTACCTCTGTAATGCATCCGGAGGCCCATGTGGTAGATACTGACATTCGTAGTCAG
GTGGAGCTCAACATGTCCGGCACCTACCACTTTTACTTCCGGTATCTGGAAAGACCCGAC
ACTGGCTGCTCCGGAGCAGATGGAGCTCTATATGTGCAAGTGGAGCCCACGCTGCATGTT
GGGCCGCCTGGCGCCCAGAAGACCATTCCCTTGGACTCGGTGCGCTGCCAAACGGTGCTG
GCCAAGCTCCTGGGACCACTGGACACTTGGGAGCCTAAGCTGCGCGTGGCAAAGGAGGCC
GGATACAATGTGATCCACTTCACTCCCATCCAGGAGTTGGGTGGCTCCCGATCCTGCTAT
TCGCTGCGTGATCAACTCAAGGTCAACTCCCATTTTGCACCCCAAAAGGGAGGTAAGATC
AGTTTTGAGGATGTGGAGAAGGTCATCAAGAAGTGCCGCCAGGAGTGGGGGGTGGCCTCC
ATCTGCGACATCGTGCTCAATCACACCGCCAACGAGTCCGATTGGCTACTACAGCATCCG
GATGCCACCTACTCATGCGCCACCTGTCCCTACCTCCGCCCCGCCTTCCTGCTGGACGCC
ACATTCGCCCAGTGCGGAGCGGACATAGCCGAGGGCAGCCTGGAGCATGTAGGCGTACCT
GCGGTCATCGAGCAGGAGTGCCATTTGGAAGCGTTAAAGTACCAGTTGCACACCTCCTAC
ATGTCCAAGGTCAATATACACGAGCTGTATCAGTGCGATGTGATGAAGTACGTGAACGAG
TTCATGTCCCAGGTGCGAACTCGCGAGCCACCGAAGAATGTGGCCAACGAGTGTCGCTTC
CAGGAAATCCAACTGATACAGGACCCGCAATATCGACGCTTGGCCAGCACCATTAATTTC
GAGCTGGCGCTGGAGATCTTTAATGCTTTCCATGGCGACTGCTTCGATGAGGAGTCACGG
TTCCGCAAGTGCGCCGAAACCCTCCGCCGGCATCTCGATGCCCTCAACGATCGTGTGCGC
TGCGAGGTTCAAGGCTACATAAACTATGCGATAGACAATGTTTTGGCCGGAGTTCGCTAC
GAAAGAGTCCAGGGCGATGGGCCCAGAGTGAAGGAGATCTCCGAGAAGCACTCGGTCTTT
ATGGTCTACTTTACGCATACCGGCACCCAGGGAAAATCGCTCACTGAGATCGAGGCGGAT
ATGTATACCAAGGCTGGAGAGTTCTTCATGGCCCACAACGGTTGGGTCATGGGATACAGC
GATCCTCTGAGGGATTTTGCTGAGGAGCAACCTGGACGCGCCAATGTCTACCTCAAGCGG
GAGCTCATCTCGTGGGGCGATAGTGTGAAGTTGCGCTTCGGCAGACGGCCGGAGGACAGT
CCCTACCTATGGCAGCACATGACCGAGTACGTTCAGACCACAGCGCGCATCTTCGACGGT
GTGCGATTGGACAACTGCCACTCCACGCCATTGCACGTAGCTGAGTACCTTCTCGATGCA
GCTCGTAAGATCAACCCAGAGCTGTATGTGGTGGCTGAACTGTTCACCAATTCCGATTAC
ACCGACAATGTGTTTGTGAACCGATTGGGTATCACCTCCTTGATCCGTGAAGCTCTTTCC
GCTTGGGACTCCCACGAGCAAGGCCGCTTAGTGTATCGGTATGGAGGAGTGCCTGTAGGT
GGTTTCCAAGCAAACTCATCGCGCCACGAGGCCACCAGTGTCGCTCATGCCCTCTTCCTG
GACCTCACCCACGATAATCCGTCTCCGGTGGAGAAGCGTTCCGTGTACGATCTTCTACCA
TCGGCGGCACTGGTTTCCATGGCATGCTGTGCCACAGGAAGTAACCGTGGTTACGACGAA
CTGGTCCCCCATCATATCCATGTCGTAGATGAGGAACGCACCTACCAAGAATGGGGCAAA
GGTGTTGACTCGAAGTCCGGAATTATGGGTGCCAAAAGAGCACTGAATTTGCTGCATGGA
CAGCTCGCAGAGGAGGGATTTAGCCAAGTTTACGTAGACCAGATGGATCCCAACGTAGTT
GCAGTTACTCGTCATTCGCCAATCACGCATCAGTCAGTTATTCTCGTGGCCCACACTGCC
TTTGGCTATCCCTCTCCTAATGCCGGACCCACCGGAATCCGCCCGCTGCGTTTCGAGGGT
GTGCTGGACGAGATCATCCTGGAGGCCAGCTTGACCATGCAGAGTGACAAGCCATTCGAT
CGTCCTGCTCCATTCAAAAAGGATCCGAATGTAATCAACGGCTTTACTCAGTTCCAGTTG
AATCTGCAGGAGCACATCCCGCTGGCTAAGTCGACAGTGTTTCAGACCCAAGCCTATTCG
GATGGCAACAACACGGAGCTAAACTTTGCCAATCTACGACCCGGCACTGTGGTGGCCATC
AGAGTGTCTATGCATCCTGGTCCTCGCACCAGTTTCGATAAGCTCCAGAAAATCTCGGCT
GCCCTGCGTATTGGATCTGGCGAGGAGTATTCCCAGCTGCAGGCTATCGTCTCCAAGTTG
GATCTGGTGGCACTTAGTGGTGCCCTTTTCAGCTGCGATGATGAGGAGAGGGATCTTGGC
AAAGGTGGCACCGCCTACGACATTCCCAACTTTGGAAAGATCGTTTACTGCGGATTGCAA
GGATTCATTTCCCTGTTGACGGAGATTTCGCCTAAAAATGACTTGGGACATCCGCTTTGT
AACAATCTGCGCGACGGAAACTGGATGATGGATTACATTTCTGATCGCCTAACTAGTTAT
GAAGACCTGAAACCACTCTCCGCCTGGTTCAAAGCTACCTTTGAGCCACTGAAGAATATT
CCACGCTACCTCATACCCTGCTACTTTGATGCCATTGTCAGCGGGGTTTACAATGTGCTC
ATCAACCAGGTCAACGAACTAATGCCAGACTTCATTAAGAATGGCCACAGTTTCCCACAA
TCCCTGGCCCTGTCCACGTTGCAGTTCCTCTCCGTTTGCAAGTCGGCCAATCTGCCGGGA
TTCAGTCCTGCTCTAAGTCCACCCAAGCCTCCAAAGCAATGTGTGACTCTATCTGCTGGT
CTGCCGCATTTCTCAACGGGCTATATGCGGTGCTGGGGCCGTGATACCTTCATCGCTCTG
CGTGGCTCCATGTTCCTCACTGGCCGTTACAACGAGGCTCGCTTCATCATCATTGGATTT
GGTCAGACCCTTCGACACGGACTCATTCCGAATCTTTTGGACAGCGGCAGCAAGCCGAGA
TTCAACTGCCGCGATGCTATCTGGTGGTGGATGTACTGCATCAAGCAGTATGTGGAGGAT
GCCCCCAAGGGTGCCGAGATCCTGAAGGACAAGGTGTCCCGCATATTTCCGTACGACGAT
GCCGATGCCCATGCTCCAGGTGCCTTCGACCAACTTCTCTTCGACGTGATGCAGGAGGCA
CTGCAGGTGCATTTTCAGGGCTTGCAGTATAGGGAGCGCAATGCAGGCTATGAGATCGAT
GCGCACATGGTGGACCAGGGCTTTAACAATCAGATCGGAATTCACCCGGAGACTGGCTTT
GTATTCGGAGGCAATAACTTCAACTGCGGCACTTGGATGGACAAAATGGGATCCTCACAG
AAGGCCGGAAACAAGGGACGTCCAAGTACGCCGCGCGACGGATCAGCCGTGGAGCTCGTT
GGCCTCCAGTATGCCGTACTCCGGTTTATGCAAAGCCTAGCCGAAAAGGAGGTTATCCCG
TACACCGGCGTGGAACGAAAGGGTCCATCGGGCGAAGTGACCAAGTGGAGCTACAAGGAG
TGGGCGGATCGCATCAAGAACAACTTTGACAAGTATTTCTTTGTATCCGAATCGGAAACC
TGCTCGGTGGCCAACAAGAAGCTTATCTACAAGGACAGCTATGGAGCCACCCAGAGTTGG
ACGGACTACCAGCTGCGATGCAACTTCCCCATCACCTTGACCGTGGCTCCCGACCTGTGC
AATCCTCAGAATGCCTGGCGTGCACTGGAGCGCGCCAAAAAGTATCTTCTGGGACCGCTG
GGCATGAAGACGATGGATCCCGAGGACTGGAACTATAGGGCCAACTATGACAACTCAAAT
GACTCCACCGATTGCACTGTAGCCCATGGCGCAAACTACCACCAGGGGCCGGAGTGGGTA
TGGCCCATCGGTTTTTACCTGCGGGCGCGCCTGATCTTCGCCAAAAAGTGTGGCCATTTG
GACGAGACCATTGCCGAGACCTGGGCCATACTACGGGCCCATCTCCGAGAGCTACAGACA
TCCCATTGGCGCGGATTGCCCGAGTTGACCAACGATAATGGCTCCTACTGCGGTGACTCC
TGTCGCACGCAGGCCTGGAGTGTTGCTGCCATTTTGGAAGTCCTGTACGATCTGCACTCC
TTGGGAGCAGACGTGGCC
Protein sequence predicted based on nucleotide sequence
MPLAMSTMGKETESHSIPISEGQDAEHILYRLKRGSKLSVHPDASLLGRKIVLYTNYPAE GQKFVRTEYRVLGWQLSNGKQITSVMHPEAHVVDTDIRSQVELNMSGTYHFYFRYLERPD TGCSGADGALYVQVEPTLHVGPPGAQKTIPLDSVRCQTVLAKLLGPLDTWEPKLRVAKEA GYNVIHFTPIQELGGSRSCYSLRDQLKVNSHFAPQKGGKISFEDVEKVIKKCRQEWGVAS ICDIVLNHTANESDWLLQHPDATYSCATCPYLRPAFLLDATFAQCGADIAEGSLEHVGVP AVIEQECHLEALKYQLHTSYMSKVNIHELYQCDVMKYVNEFMSQVRTREPPKNVANECRF QEIQLIQDPQYRRLASTINFELALEIFNAFHGDCFDEESRFRKCAETLRRHLDALNDRVR CEVQGYINYAIDNVLAGVRYERVQGDGPRVKEISEKHSVFMVYFTHTGTQGKSLTEIEAD MYTKAGEFFMAHNGWVMGYSDPLRDFAEEQPGRANVYLKRELISWGDSVKLRFGRRPEDS PYLWQHMTEYVQTTARIFDGVRLDNCHSTPLHVAEYLLDAARKINPELYVVAELFTNSDY TDNVFVNRLGITSLIREALSAWDSHEQGRLVYRYGGVPVGGFQANSSRHEATSVAHALFL DLTHDNPSPVEKRSVYDLLPSAALVSMACCATGSNRGYDELVPHHIHVVDEERTYQEWGK GVDSKSGIMGAKRALNLLHGQLAEEGFSQVYVDQMDPNVVAVTRHSPITHQSVILVAHTA FGYPSPNAGPTGIRPLRFEGVLDEIILEASLTMQSDKPFDRPAPFKKDPNVINGFTQFQL NLQEHIPLAKSTVFQTQAYSDGNNTELNFANLRPGTVVAIRVSMHPGPRTSFDKLQKISA ALRIGSGEEYSQLQAIVSKLDLVALSGALFSCDDEERDLGKGGTAYDIPNFGKIVYCGLQ GFISLLTEISPKNDLGHPLCNNLRDGNWMMDYISDRLTSYEDLKPLSAWFKATFEPLKNI PRYLIPCYFDAIVSGVYNVLINQVNELMPDFIKNGHSFPQSLALSTLQFLSVCKSANLPG FSPALSPPKPPKQCVTLSAGLPHFSTGYMRCWGRDTFIALRGSMFLTGRYNEARFIIIGF GQTLRHGLIPNLLDSGSKPRFNCRDAIWWWMYCIKQYVEDAPKGAEILKDKVSRIFPYDD ADAHAPGAFDQLLFDVMQEALQVHFQGLQYRERNAGYEIDAHMVDQGFNNQIGIHPETGF VFGGNNFNCGTWMDKMGSSQKAGNKGRPSTPRDGSAVELVGLQYAVLRFMQSLAEKEVIP YTGVERKGPSGEVTKWSYKEWADRIKNNFDKYFFVSESETCSVANKKLIYKDSYGATQSW TDYQLRCNFPITLTVAPDLCNPQNAWRALERAKKYLLGPLGMKTMDPEDWNYRANYDNSN DSTDCTVAHGANYHQGPEWVWPIGFYLRARLIFAKKCGHLDETIAETWAILRAHLRELQT SHWRGLPELTNDNGSYCGDSCRTQAWSVAAILEVLYDLHSLGADVA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
