Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
1258
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00764172
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3249nts
Open reading frame: 1 to 3249
ATGTCGAGCTTTGTGCTGGACAAGGAGGCGTTCGTGCGCCGCGTGAAACGCCTCTACACG
GAATGGAGGGCTCCCAGCATCGGACACGATGACGCGCTCAGGAATCTGGACTGCATCATG
AGCATCGTGGGCGTCGAAGAGGATGTCATGTACTCCAAGTCCATGGCCCTGCAGCTCTGG
CTGCTGGGCTACGAGCTGACGGACACCATCTCAGTGTTTTGCTCGGATGCCGTGTATTTT
CTCACTAGCAAAAAGAAAATAGAATTCCTGAAGCAGACGCAGAACATCACCGAAGAAGGT
TTTCCGGAAATCAATCTGCTTGTTAGGGATCGGACGGACAAGGACCAGGGCAACTTCGAG
AAGCTCATCAAAGCCCTGCAAAACTCGAAGAAGGGCAAGCGTTTGGGCGTCTTCGCCAAG
GACGCCTATCCCGGGGAGTTCAGCGAAGCATGGAAGAAATCCCTGACGGCGTCCAAGTTC
GAGCATGTGGACATTAGCACTATTATCGCCTACCTCATGTGTCCCAAGGATGAGTCGGAG
ATCAACAACATACGCAAGGCATCGCTAGTGTCAATGGACATTTTCAACAAGTATTTGAAA
GATGAGATCATGGACATTATTGATTCGGATAGGAAAGTCAAACACAACAAGCTCTCAGAT
GGCTGCGAGGCTGCCATTGGCGAGAAGAAGTACACCAGTGGTCTGGATCCCAGACTCCTC
GATATGGCCTACCCACCCATCATCCAATCGGGAGGAGCCTACAGCCTGAAGTTCTCCGCG
GTGGCGGACAAAAATCCCTTGCACTTCGGTGTGATTGTGTGCTCCTTGGGCGCCCGCTAC
AAGTCGTATTGCTCAAATATATCGCGCACCTTTCTGGTGAATCCCACCGAGGCCATGCAG
GAGAACTACACATTTCTGGTAAGTGTGCAGGAGGAGATTCTCAAGCTGCTGGTTCCGGGC
ACAAAGCTGTGCGACGTCTACGAAAAGACCCTGGACTTCGTGAAGAAGGAGAAGCCGAGT
ATGGTGGACAATCTGCCCAAAAGCTTTGGTTTCGCCATGGGCCTAGAGTTCCGGGAGAAC
TCCATTGTCATCGGACCCAAATGCCAGGCGCTACTCAAGAAGAATATGGTCTTTAATTTG
CACGTGGGCATCTCGAATCTGACCAATCCCGAAGCCACCGACAAAGAGGGCAAGAACTAC
GCCCTCTTCATCGGAGACACTGTGCTAGTAGGCGAACAATCCCCAGCCAGTGTGATGACT
CCGTCCAAGAAGAAGATCAAGAACGTCGGCATCTTTATCAAGGACGACAGCGACGAGGAA
GATGTGGACGACAAGAAGACCGCTAAAGAAGATCAGGGCACCGAGATCCTGGGCCGCAGC
AAGCGCAACGCGGTGCTGGAGTCAAAGCTGCGAAACGAGATCAACACGGAGGAAAAGCGC
AAGGAGCACCAGCGCGAGCTGGCTCAGCAGCTGAACGAGCGCGCCAAGGATCGGCTGGCC
AGGCAGGGAAACTCGAAGGAGGTCGAGAAAGTGCGCAAGAACACCGTCTCCTACAAGTCC
ATCAGCCAAATGCCGCGCGAGCCGGAAGTCAAGGAGCTCAAGCTTTACGTGGATAAGAAG
TACGAGACCGTCATCATGCCAGTGTTCGGCATCCAGGTGCCGTTCCATATATCCACCATC
AAGAACATCTCGCAGTCGGTGGAGGGCGAGTACACGTACTTGCGTATCAACTTCTTTCAT
CCCGGCGCCACAATGGGTCGCAACGAAGGCGGACTCTATCCTCAGCCAGAGGCCACATTT
GTCAAGGAAGTTACATACCGCAGCTCTAATGTTAAGGAACACGGGGAGGTGGGAGCTCCA
TCCGCAAATCTAAACAATGCTTTCCGGCTGATCAAGGAGGTGCAGAAGCGCTTCAAGACC
CGCGAGGCTGAGGAGCGCGAAAAGGAGGATCTTGTGAAGCAAGACACACTCATTCTGTCC
CAGAACAAGGGTAATCCCAAGCTGAAGGACCTATACATTCGGCCCAACATCGTGACCAAG
CGCATGACGGGCAGCCTGGAAGCGCACAGCAATGGATTCCGCTACATCTCCGTGCGCGGC
GACAAGGTGGACATCTTGTACAACAACATCAAAAGCGCGTTTTTCCAGCCCTGCGATGGC
GAGATGATCATTCTTCTGCATTTCCACCTGAAGTACGCCATTATGTTTGGCAAAAAGAAG
CACGTGGATGTACAGTTCTACACGGAAGTGGGAGAGATCACCACGGATCTGGGCAAGCAC
CAGCACATGCACGACCGCGACGACTTGGCTGCTGAGCAGGCAGAACGTGAGCTGCGGCAC
AAGCTCAAGACTGCCTTCAAGAGCTTCTGCGAGAAGGTGGAAACCATGACCAAGTCGGTA
GTGGAGTTCGACACGCCGTTCCGTGAGTTGGGATTCCCTGGAGCACCCTTCCGCAGCACG
GTGACGCTGCAACCCACCTCGGGCTCCTTGGTCAATCTGACGGAATGGCCACCATTTGTC
ATCACGCTGGACGATGTGGAGTTGGTGCACTTCGAGCGCGTTCAGTTCCACTTGCGCAAC
TTCGATATGATATTTGTGTTCAAGGAGTACAACAAGAAGGTGGCCATGGTGAACGCCATT
CCTATGAACATGCTGGATCACGTGAAGGAGTGGCTCAACTCCTGCGACATCCGCTATTCG
GAGGGTGTTCAATCCTTGAACTGGCAAAAGATCATGAAGACCATCACCGACGATCCTGAG
GGATTCTTTGAACAAGGCGGCTGGACATTCTTGGACCCGGAATCGGGCAGCGAAGGCGAA
AACGAAACCGCCGAGTCCGAAGAGGACGAGGCCTACAATCCCACCGACGCTGAATCGGAT
GAGGAGTCGGACGAAGATTCCGAGTACTCAGAGGCATCAGAAGACAGCGAGGAAAGCGAT
GAGGACCTTGGCTCTGATGAGGAATCTGGCAAGGATTGGTCCGATCTGGAGCGCGAGGCT
GCTGAGGAAGACCGCAACCACGATTATGCCGCAGATGACAAACCACGCAACGGCAAATTC
GATTCGAAGAAGCACGGCAAGAGTTCAAAGCACAGCCCGTCGAAGTCGTCGAAGGACAAG
TACAACAGCCGGGACAAGCATCATTCCTCCTCATCGTCCGGAAACAAGAGCAGCAGCAAG
GACAAGGATCGCAAGCGCTCGCGGGATGACAGTCGCGACAATGGGCACAAGTCGAAGAAG
TCGCGACAT
Protein sequence predicted based on nucleotide sequence
MSSFVLDKEAFVRRVKRLYTEWRAPSIGHDDALRNLDCIMSIVGVEEDVMYSKSMALQLW LLGYELTDTISVFCSDAVYFLTSKKKIEFLKQTQNITEEGFPEINLLVRDRTDKDQGNFE KLIKALQNSKKGKRLGVFAKDAYPGEFSEAWKKSLTASKFEHVDISTIIAYLMCPKDESE INNIRKASLVSMDIFNKYLKDEIMDIIDSDRKVKHNKLSDGCEAAIGEKKYTSGLDPRLL DMAYPPIIQSGGAYSLKFSAVADKNPLHFGVIVCSLGARYKSYCSNISRTFLVNPTEAMQ ENYTFLVSVQEEILKLLVPGTKLCDVYEKTLDFVKKEKPSMVDNLPKSFGFAMGLEFREN SIVIGPKCQALLKKNMVFNLHVGISNLTNPEATDKEGKNYALFIGDTVLVGEQSPASVMT PSKKKIKNVGIFIKDDSDEEDVDDKKTAKEDQGTEILGRSKRNAVLESKLRNEINTEEKR KEHQRELAQQLNERAKDRLARQGNSKEVEKVRKNTVSYKSISQMPREPEVKELKLYVDKK YETVIMPVFGIQVPFHISTIKNISQSVEGEYTYLRINFFHPGATMGRNEGGLYPQPEATF VKEVTYRSSNVKEHGEVGAPSANLNNAFRLIKEVQKRFKTREAEEREKEDLVKQDTLILS QNKGNPKLKDLYIRPNIVTKRMTGSLEAHSNGFRYISVRGDKVDILYNNIKSAFFQPCDG EMIILLHFHLKYAIMFGKKKHVDVQFYTEVGEITTDLGKHQHMHDRDDLAAEQAERELRH KLKTAFKSFCEKVETMTKSVVEFDTPFRELGFPGAPFRSTVTLQPTSGSLVNLTEWPPFV ITLDDVELVHFERVQFHLRNFDMIFVFKEYNKKVAMVNAIPMNMLDHVKEWLNSCDIRYS EGVQSLNWQKIMKTITDDPEGFFEQGGWTFLDPESGSEGENETAESEEDEAYNPTDAESD EESDEDSEYSEASEDSEESDEDLGSDEESGKDWSDLEREAAEEDRNHDYAADDKPRNGKF DSKKHGKSSKHSPSKSSKDKYNSRDKHHSSSSSGNKSSSKDKDRKRSRDDSRDNGHKSKK SRH
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
