Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
7108
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00764277
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3681nts
Open reading frame: 1 to 3681
ATGGAATTCGACGACGCAGAAAATGGCCTAGGAATGGGATTTGGTGGTCGCGGCGTTCTG
CGGGAGATCAACAAAAACGATGGCGGCATTAAGCTGAGTCCGTTGGCGGTGAAGCCCAGT
CCCAAGCCAACGAGCATGGATCAACAGAAGGCAATGGCGGCAGCTCTGGGGCATCAGACG
GCGGTACCCGGTGGACTTAAGCCAGAAGACGACCTCGTTCCAAAGTTTGGAGGTCTTCCC
GAGTTCGATGAGACGCAAATACCGGAACTAGCTCCTTTAAGTACCGATATGGATACTCAA
CTGAAATCCAACCATTCGGATTCGGATGTTTTTGTCGAGTGTTTATCGCTTCATAGCGAG
CGCTACACCGGAGATCGATCGGAGTTAGAGGCATATTCAAGTGCCCTTAATGATGTTTTG
GAGCAGCAGCTGTCCATGACCTCAGTTTCTACACTGGGAGCTGAATTCAACGATCCTTTG
GCAGTTAGCCAGCACAACAACGTCAGCTATGAAGCCATGGATGTGGATGACCTAAACGAA
ACCATGGAAATGTTAAAGGACGTCTTGAATGCGGAAGACCGTCATCTTATGCAAACGCAT
ATTCTCAGCGTAACCCAGTCAAAACCAGAGAATAATTTTGTGCACTATCATCCAAATCAG
GAGCAAAATAGGGTTGAACTAGACCAGTTAGCCAAACACTCTAAACCCAATAGTGATGCA
AAGGAATCAGAATCATTGGTAGAATCAGTACTGTCTGAGGAGTCTGGTACACAATCCTTT
GAGCCGAAGCTCACCTTATCTGCCTTGGACTCTACGAAAGACTTAGCTAAGCATCAAGAG
GAAATGCAAACAAATACAAAAGATATATTTCAGAAAGAGAAGCACCACCCGGTCAGTAGT
GCTCAGGTGAAGGACGCACAAAATCCTAAGGAGCCTGAAAAGGCCTTAAATCTCAAAGAG
GCTTCTTCAGAACTGTCCACAAAATCAGCAAAATCTCTAGAATACCCTAATCAAGAGAAG
CAATTTTCGGTGGCAGTAGAATCAATGGAGAGACCATGCACTGCCATCAGCCTTGAGCAA
AATGCATCCCCAGCTATGGAGGCAACTGAAGACAGGACTGTGGAGGAGCTTGATCAAATG
GATGTGGATGAATCCATGAAAGTGGATGATGCCACAGTGCTTAACTCATGTCGGCAGGAG
CAAATTAATGCTCATTCTCCCGTTTCAAGCAAATTTTTCTTAAAACTCTGCGTTCAAAAT
GATACGGATTGTCAAGATAATAAAATGGATCAAAAAACTGCAGGAAAAGAAAGTGCATTA
AAGATGACTGAAATAGAGCTGCCTGTAGAATCTGTCCCATCAGCTTCGCTCTCACCGCCG
ACACCCGCACTTCAAGCAAAAATTATCGAACTTTCCCTTTCCCCGCCCATACCTACACAT
CGCCCAAAAACAGCGGAGGAACTAGAGTTCCTGGCTTTGAGGGAGCTGCCTTTGCCTGAT
GGTGTCTCGACGGCGGGGAAGGTGGAATGTAAGCAGCTATCACACGGCCTGGTACCCATT
GTGGCAGTCTTACCAACGCCACTTTACTTGGATCTGAAAGGGAATACAACCACTGCAAGC
CTTCCAGAACCGACTTCGCCCAGTTTCCCCATCAAAGAACCTGCTGAAAAACAGTCCCAC
TTTCCACGCTCACCGCGTGCTCAGCTCGAGCAAAAGGACATGCCCTATTTGGAAGAGGCT
GTGGTAGAACGTTCATCACATCGCAAGCAAAGCGATTTCGGAGTCATAGCGAAATCACCA
ATAGTCTTTGAGTTAACACAACCGAGTCCTGAGAAGCAGCACCTCAACGAAACCTTGCCA
ATGAGCGAGATTTCTCCCACTTCGTCAAATATGACCTTTGACAGCGGAAGCATTACGGAG
GACAAATCACAGAAGGGTACAGAGAATATTGAGCCTTCTTCCACCCTTGCCGTAAGTCTG
GGATCGGTTGATGATAAGCAGCGGCGCACTTTCTCGGTGGTTCAGCCCACGAAGGAAGAG
GAGGAGGCCAAATCGCGTCGGACTTTCTGTATGGAAAATGAGAACCCTCGACGCACCTTC
TGCATGCAGCAGAATGCTTCTCCAGCGGTGGAGGCTACCGAAGACACCGGGGCTGATGAA
GTTATGGATGTGGATATCTCCATGAGGGCAGATGAAGTAGCCGTGATGAAGTCGCATCAG
CAAACTTGGAAGGAACGCCAAGCACCAATTTCAAATTCACCACCAATTCCTACTCACCAA
CGTTTAAATAGACCTCCTGAGGCGATTTCTCCAGTGTCCCCATTGGGAAATGGCACCGTT
GTGTTGGAGGAGCAAACTCTCTCTGCTAAAGAGCAAGCCACGCTAAGTGCCAGCGATGAG
AAGGACGATGTTTTCTTGGAGCACTTTGGTGCAATTTCCCCAGTTTCGGATGACATGTTT
AAAACCAGCAGCGCTTCCAACAATCAAGCAAAGTTTAGGGTCAATGCTGATGAAGAAACC
GGAGGCAAAGGCGCTTCTGCGATGATCAGGAAGAGAAGTGGTACAGCTGGCGAGCAGGAA
GAGATCTTTGATGCCGAATTCCATGATGGCGTCAACAACCAAAACCTAATACTAAATTCA
TCAGATTTTGATTATTTGTACACGAAAGGCAGCAATAGTGTGCCTATTGACCGCAGTTCG
CTGCTGTTGAAGTTTGATCCCCTCCTTGGAGCCCCTGTTCCCGTTAATCATCCGAATCAG
CAGGAGCAGGCGCTGCAGAATATTCTGGGATCGAACCAGCACCAGAACCGGCTCCTTAGC
CCAACATTGGAGGAGCACGAAACAAGTGACGGGAACCAGTCTTTTGGAGTAATATCGAGT
GCCAAAGATACAGCCAAGAAATGGGATTTTAAGCCACCTGTGGATAGAACAAAAAAGCAT
GTTAAAATGAGTGTGGACGTCATTGATAACGATTGCAATAAAACCTTCGATAATTCCAAC
ACTAATACGGAGGATAAGACGCACAACTACAACGATATGGATGAGCTGGAGAAGAAAATC
AAAAATGAAGTAACGAGGTCCGAAGACATTGAAAAGAAGCTCAAGGAGGGAGAGCTGCGC
GAGGAGGCTCTGATCAAGCGTATTACAGAAAAGGACAAAACAAATGCAAAACTAAACGGT
GTCATTGAGGCATATGAGAAGGCAATTGCAGAGCTCATTAGTGAGAAGGAGCAGCAAGCA
CAGCTTCACGAACGGCAGTTGCAGGAAGTCCAGGCAGACCGGGACGCAAATTACCATCAC
TTAACATCGTTGGAGACGACATTTTCCGATCTGCATGTGAAATACGAGAAAAGCAAAGAG
ATGACCTCGCAGCTTAAAAGTAACGAGGAATCGCTTCTGGCAGAGCGAAAGCAGATGATG
GACAATCTGCGGTTGCAGGAACAGCGCTACGACAAGATGAAGAACCATGCCATGCAGCAG
CTGGAAATTGCCAACAAAAAGCTGGACACCTACTCGAGGGAGCATGCCGACGAGACGAAA
AAACTTAAAGCTTTACTTAAGAAGGAGGAGATCTCGCGGGTCTCGATGACGGAGCAGCTG
CAACAGAAGTCCCGCGAAAACGCTGATCTGCTCAAGATCTGCGAAGAGCTCATCTATGGC
AAGGGACAAGGTGGTAGTAGT
Protein sequence predicted based on nucleotide sequence
MEFDDAENGLGMGFGGRGVLREINKNDGGIKLSPLAVKPSPKPTSMDQQKAMAAALGHQT AVPGGLKPEDDLVPKFGGLPEFDETQIPELAPLSTDMDTQLKSNHSDSDVFVECLSLHSE RYTGDRSELEAYSSALNDVLEQQLSMTSVSTLGAEFNDPLAVSQHNNVSYEAMDVDDLNE TMEMLKDVLNAEDRHLMQTHILSVTQSKPENNFVHYHPNQEQNRVELDQLAKHSKPNSDA KESESLVESVLSEESGTQSFEPKLTLSALDSTKDLAKHQEEMQTNTKDIFQKEKHHPVSS AQVKDAQNPKEPEKALNLKEASSELSTKSAKSLEYPNQEKQFSVAVESMERPCTAISLEQ NASPAMEATEDRTVEELDQMDVDESMKVDDATVLNSCRQEQINAHSPVSSKFFLKLCVQN DTDCQDNKMDQKTAGKESALKMTEIELPVESVPSASLSPPTPALQAKIIELSLSPPIPTH RPKTAEELEFLALRELPLPDGVSTAGKVECKQLSHGLVPIVAVLPTPLYLDLKGNTTTAS LPEPTSPSFPIKEPAEKQSHFPRSPRAQLEQKDMPYLEEAVVERSSHRKQSDFGVIAKSP IVFELTQPSPEKQHLNETLPMSEISPTSSNMTFDSGSITEDKSQKGTENIEPSSTLAVSL GSVDDKQRRTFSVVQPTKEEEEAKSRRTFCMENENPRRTFCMQQNASPAVEATEDTGADE VMDVDISMRADEVAVMKSHQQTWKERQAPISNSPPIPTHQRLNRPPEAISPVSPLGNGTV VLEEQTLSAKEQATLSASDEKDDVFLEHFGAISPVSDDMFKTSSASNNQAKFRVNADEET GGKGASAMIRKRSGTAGEQEEIFDAEFHDGVNNQNLILNSSDFDYLYTKGSNSVPIDRSS LLLKFDPLLGAPVPVNHPNQQEQALQNILGSNQHQNRLLSPTLEEHETSDGNQSFGVISS AKDTAKKWDFKPPVDRTKKHVKMSVDVIDNDCNKTFDNSNTNTEDKTHNYNDMDELEKKI KNEVTRSEDIEKKLKEGELREEALIKRITEKDKTNAKLNGVIEAYEKAIAELISEKEQQA QLHERQLQEVQADRDANYHHLTSLETTFSDLHVKYEKSKEMTSQLKSNEESLLAERKQMM DNLRLQEQRYDKMKNHAMQQLEIANKKLDTYSREHADETKKLKALLKKEEISRVSMTEQL QQKSRENADLLKICEELIYGKGQGGSS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
