Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
231
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00764633
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4188nts
Open reading frame: 1 to 4188
ATGACTTATGGTGGCCAAAGGGTTCCCCTGGGCGAGAAGGTCGTCGTGGAACAAACGGTG
GATGTATCTAAGATCAAGGTGGACGGACTTGAGCCAAGTGTGATCATGAATGCGGCCACG
GACTTCATGGTTGATATGAGCAAGGTGGGCAGCAACATCGACTCAGGAAAGCTGTCCTGT
GCGATCTTCGACCCAATGGGCCATGTGTTGCCAAGCAAGATTGTCCAAGGTCCAACCGAC
GACATCTTCCGCATCATGTACACTCCATTCGAGGCTGGCCGCCACACCATTGAACTGATG
TACGACAACATCCCAGTGCCAGGATCTCCGTTTGTTGTGAATGTGAAGAGCGGCTGTGAT
CCTGCTCGCTGCAAGGCCTACGGACCAGGCCTCGAAAAAGGACTGACCAATCAGAAAAAC
AAATTCACCGTGGAAACCAAGGGTGCAGGTAATGGTGGCCTTTCGCTTGCCATCGAGGGT
CCTTCGGAGGCAAAAATGACGTGCACGGACAATCGCGACGGTAGCTGCGACGTTGACTAT
TTGGCCACTGATCCAGGAGAGTATGACATTACCATTCGGTTTGCGGACAAGCACATACCC
GGCTCTCCATTCCGCGTGCTCGTCGAGGAGACTGTGGATCCCAGCAAGGTGAAGGTGTAC
GGTCCGGGCATCGAGCACGGCCAGGTGCGCGAAAGTGTGCCCACCTTCTTCAACGTGGAT
GTGGGCGAGGCTGGTCCTGGCCGCATTGCCGTTAAGCTGACCAACTCCGAAGGTATTCCC
GTGGACAATCTACGCGTGGAAGACAAGGGCAATTGCATTTACGCGGTTCACTATGTGCCG
CCCAAGGCTGGCTCCGTGCTCACCTGCCAGGTGAAGTTCTCCGAGGTTGAAGTGCCATGC
AGTCCATTTGTGATGACCGTTTTCCCGAAATCAGAACCCACCAAGGTTAAGGTAAAGGGT
GTCAATGAGAAGAAGAAAACGCCTGCTTCCCTCCCCGCAGAGTTTGAAATCGATACAAAA
CAAGCCGGTCAGGCTGATATCAATGTGGCCATTAAGAACCCCAAGGGCAAGGCGATGCAG
CCGCGCCTGGAGGAGGTGTCCACCGGCACGTACGTGGTGTCCTTTGTGCCAGATGAGTGT
GGCACCTACCAGTGCAGCATCAAGTACGGCGACAAGGAGATCGAGGGCTCACCCTTCAAA
CTCGAAGCATTCCCCACCGGCGAAGCCAAGAAATGCAAACTGGTGGAGCAGGCGCCCAAG
ATTCAGACCTCGGGCAGTCAATCGCACCTAAAAGTGGATGCTCGTGAGGCCGGAGATGGA
GCGGTGACCTGCAAGATCACCAATAAGGCGGGCAGCGAAATTGTGGACATTGATGTGATC
GAAAAGGATGGTTTCTTTGACATTCTGTACGCCCTTAACGATCCCGGAGATTATGACATC
AACGTAAAGTTCGGCGGCAAGGACATACCGAACGGCAGCTTCTCCATCAAGGCTGTCGAA
AGTATCGAGCAATACTCGCATTCTGAATACATCGAGGAGCACACGACCAAAGTGGTGCAG
CAAACTACGCAGAGCGAGCTTGTCAACGGAAAATCTGAGATCACCTATCGCAGTGTAGCA
TTTGAGAAATTACCACTACCCACAACAGGCGGCAACGTTACAGCTGAAGTCAGAATGCCT
AGCGGTAAAGTAGACAAACCCGTGATCCAGGACAACCGTGATGGTACCGTCTCGGTGAAG
TACGATCCCCGTGAGGAGGGATCCCACGAGCTGGTGGTCAAATACAACGGAGAACCCGTG
CAAGGATCTCCCTTCAAATTCCACGTTGACTCGATCACCTCCGGCTATGTGACTGCCTAC
GGACCCGGCCTGACCCACGGAGTCACCGGTGAGCCGGCCAACTTTACCATCTCCACCAAG
GGAGCCAGCGCCGGTGGCCTGACCATGGCCGTCGAAGGACCCAGCAAGGCTGACATCAAC
TACCATGACAACAAAGACGGCACTGTATCTGTGCAATACCTGCCCACTGCTCCTGGCGAG
TACCAGGTGTCCGTTCGCTTCGGCGATAAGCACATCAAGGGATCGCCGTACTTCGCCAAG
ATCACCGGCGAGGGTCGCAAGCGCAACCAGATCTCGGTTGGCTCCTGCTCCGAAGTGACC
ATGCCCGGCGACATTACCGATGACGATCTGCGCGCTTTGAACGCCTCGATCCAGGCGCCC
AGTGGCCTGGAGGAGCCCTGCTTCCTGAAGCGCATGCCCACTGGCAACATTGGCATCTCG
TTCACGCCCCGCGAGATTGGCGAGCATCTGGTGTCGGTGAAGCGTCTGGGCAAGCACATC
AACAACTCACCATTTAAGGTGACTGTCTGCGAGCGCGAGGTGGGCGACGCCAAGAAGGTC
AAGGTTAGCGGAACTGGCCTCAAGGAGGGTCAAACCCATGCCGACAACATCTTCTCCGTG
GACACGAGGAACGCCGGCTTCGGTGGTCTTTCGGTCTCCATTGAGGGTCCCAGCAAGGCT
GAGATCCAGTGCACGGATAAGGATGACGGTACTCTCAACATCTCGTACAAGCCCACTGAG
CCGGGCTACTACATTGTTAACCTGAAGTTCGCCGATCACCACGTGGAGGGTTCTCCTTTC
ACCGTCAAGGTTGCCGGCGAGGGCAGCAACCGCAAGCGCGAGAAGATCCAGCGGGAGCGC
GATGCTGTTCCAATCACGGAAATCGGAAGCCAGTGCAAGCTGACATTCAAGATGCCGGGC
ATTACCTCGTTCGATCTGGCCGCCTGTGTCACCTCGCCTAGCAACGTGACCGAGGATGCG
GAGATCCAAGAGGTGGAGGATGGCCTCTACGCAGTGCACTTTGTGCCCAAGGAGTTGGGC
GTGCACACGGTGTCGGTGCGCTATTCCGAGATGCACATACCCGGATCACCGTTCCAGTTC
ACCGTGGGACCACTGCGCGACTCCGGCAGCCATCTTGTTAAGGCTGGAGGCTCTGGTCTG
GAGCGAGGTGTTGTCGGAGAGGCGGCCGAGTTCAATGTGTGGACCCGCGAGGCAGGCGGT
GGTTCCCTGGCCATTTCCGTGGAAGGTCCCAGCAAGGCTGACATCGAGTTTAAGGACCGC
AAGGACGGCAGCTGCGATGTGTCGTACAAGGTCACCGAACCGGGAGAGTACCGCGTGGGC
CTGAAATTCAACGATCGCCACATACCCGACTCACCATTCAAGGTGTACGTCTCCCCGGAT
GCGGGTGATGCCCACAAGCTGGAGGTTCAGCAGTTCCCGCAGGGTAACATCCAGGCGGAC
GCTCCCTACCAATTCATGGTCCGCAAGAACGGTGCCAAGGGTGAGCTGGATGCCAAGATT
GTGGCTCCATCCGGAACGGACGATGATTGCTTCATCCAGGTGATCGACGGCGAGATGTAC
TCGGTGCGATTCTATCCACGCGAGAACGGAATCCATGCCATCCACGTCAAGTTCAACGGC
GTCCACATACCCGACTCTCCATTCAGGATCAAGGTGGGCAAGGATGTGGCCGATCCAGCT
GCCGTGCACGCCAGCGGCAATGGATTGGACGAAGTGAAGACTGGACACAAGGCCGACTTC
ATCATCAACACCTGCAACGCCGGCGTTGGCACGTTGGCCGTCTCCATCGATGGACCCTCC
AAGGTGGCCATGGACTGCACAGAGGTTGAGGAGGGCTACAAGGTCCGCTACACCCCGCTC
CTGCCCGGCGAGCACTACATCACGGTCAAGTACAACAACATGCACATCGTGGGCTCGCCA
TTCAAGGTGAACGCTACCGGCGACAAGTTAGCGGATGAGGGCGCCCAGGAGACGTCCACG
GTGATCGTGGAGACCGTGCAGAAGGTGGCCAAAGGAGGCAAGAACACGGGCGTCCATCTG
CCCACCTTTAAGTCGGATGCCAGCAAGGTGGTGTCCAAGGGTATGGGCCTGAAGAAGGCC
TACATTGGCAAGCAGAACCAGTTCAGCATCAGCGCCACCGATGCGGGCAACAACATCCTG
TACGTGGGCATGTACGGACCAAAGGGACCCTGCGAGGAGTTCCACGTGAAGCACGCTGGC
CACAACAACTATAATGTGCAGTATCTGGTGCGCGATCGCGGCCAGTACGTGCTCCTAATC
AAGTGGGGCGAGGAGCATATACCCGGCTCCCCATTCCAGATCGATGTG
Protein sequence predicted based on nucleotide sequence
MTYGGQRVPLGEKVVVEQTVDVSKIKVDGLEPSVIMNAATDFMVDMSKVGSNIDSGKLSC AIFDPMGHVLPSKIVQGPTDDIFRIMYTPFEAGRHTIELMYDNIPVPGSPFVVNVKSGCD PARCKAYGPGLEKGLTNQKNKFTVETKGAGNGGLSLAIEGPSEAKMTCTDNRDGSCDVDY LATDPGEYDITIRFADKHIPGSPFRVLVEETVDPSKVKVYGPGIEHGQVRESVPTFFNVD VGEAGPGRIAVKLTNSEGIPVDNLRVEDKGNCIYAVHYVPPKAGSVLTCQVKFSEVEVPC SPFVMTVFPKSEPTKVKVKGVNEKKKTPASLPAEFEIDTKQAGQADINVAIKNPKGKAMQ PRLEEVSTGTYVVSFVPDECGTYQCSIKYGDKEIEGSPFKLEAFPTGEAKKCKLVEQAPK IQTSGSQSHLKVDAREAGDGAVTCKITNKAGSEIVDIDVIEKDGFFDILYALNDPGDYDI NVKFGGKDIPNGSFSIKAVESIEQYSHSEYIEEHTTKVVQQTTQSELVNGKSEITYRSVA FEKLPLPTTGGNVTAEVRMPSGKVDKPVIQDNRDGTVSVKYDPREEGSHELVVKYNGEPV QGSPFKFHVDSITSGYVTAYGPGLTHGVTGEPANFTISTKGASAGGLTMAVEGPSKADIN YHDNKDGTVSVQYLPTAPGEYQVSVRFGDKHIKGSPYFAKITGEGRKRNQISVGSCSEVT MPGDITDDDLRALNASIQAPSGLEEPCFLKRMPTGNIGISFTPREIGEHLVSVKRLGKHI NNSPFKVTVCEREVGDAKKVKVSGTGLKEGQTHADNIFSVDTRNAGFGGLSVSIEGPSKA EIQCTDKDDGTLNISYKPTEPGYYIVNLKFADHHVEGSPFTVKVAGEGSNRKREKIQRER DAVPITEIGSQCKLTFKMPGITSFDLAACVTSPSNVTEDAEIQEVEDGLYAVHFVPKELG VHTVSVRYSEMHIPGSPFQFTVGPLRDSGSHLVKAGGSGLERGVVGEAAEFNVWTREAGG GSLAISVEGPSKADIEFKDRKDGSCDVSYKVTEPGEYRVGLKFNDRHIPDSPFKVYVSPD AGDAHKLEVQQFPQGNIQADAPYQFMVRKNGAKGELDAKIVAPSGTDDDCFIQVIDGEMY SVRFYPRENGIHAIHVKFNGVHIPDSPFRIKVGKDVADPAAVHASGNGLDEVKTGHKADF IINTCNAGVGTLAVSIDGPSKVAMDCTEVEEGYKVRYTPLLPGEHYITVKYNNMHIVGSP FKVNATGDKLADEGAQETSTVIVETVQKVAKGGKNTGVHLPTFKSDASKVVSKGMGLKKA YIGKQNQFSISATDAGNNILYVGMYGPKGPCEEFHVKHAGHNNYNVQYLVRDRGQYVLLI KWGEEHIPGSPFQIDV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
