Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
8688
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00765027
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3039nts
Open reading frame: 1 to 3039
ATGGGTGAAGTAGCTGCCACAGTGGAGCCTCTGGCCGCGGTCAAGTCGAAATCCATGCGA
TCCCGGAAGAGACGACAGCGTCGTAGGCGACAAATAGCCTACCTGGCCATATGTGGATTA
AGTGTAGCCATATTCGGCTTCGCTTTGGCCACGCTGATCCGGCCCACAACCGCCCAGGCT
GATGCAGATCCCGGGAGCTGGCGCAAGGGATATGTGGGCCATGGCACCACGCACGAACAG
CTTGGCCAGCCCCATTGGCCACCAGTCCAGTACACTGTATACCGGCCGACAACCAACTAC
ACAAAGGCACCACCACCTCCCACGTCCGCCATGAACCCCATTTTCAATTTTACACACTTT
CTATATGACAAGGTTCTTTATAGGGATGAACCCATTCCGGAGGGTTACATTGTCGTAAAG
AATTCGGACACTTTGAGCTTGGGTCCCAAAGTGGAGGAGAATGACTGGCGGGATCTATTG
GCTCATTATTGGATGGTTCTCATCTGGGTTGTTATTCTCGTGGTGCTCATTATAGTCATA
CCGTTCATTGCCGTTTGCTATTGCTGTTTCTGTTGCTGCCGACGATGTCGACAGGGATGT
CCTCCGTGTACCAGTAAACAGGATGCTCAGCGGCGCTTCTGTTGTGGCATCTGTCTGTTG
ATCCTCATCATCGGACTGATTTTTGGCATCATTATCGCCTTTGTTACCAACAAAATGATT
GACAGCGGCTTTGCCGAGACTTCCGAGACAATGAAGCGCGGCAGCGAGGACACTTGCACT
TATCTGAAGGATGTAGCCGATCACGTTCACCATCTGATGATGTACAACTACGAGGAGATG
GAGACTCATGTGCTTGATCAATTAACCCACGCTCACCGGCACATATTTTTGGATCTCTCC
GACACATCGGAGAGTAATTCCTTGGCAGAAATGGAGCGAGTTTTGGAGAACATGCCCGAG
GCTCTGGAGCTGATGAGGCAAGTGGAGAAGATGGAGAAAGATCTGCGCTTCTATGGCTCC
CAATTAAGAGATGGCGTCCGTGGCATTAAACGAGATGTCAACTTTGCAGTGGCCAATCTC
TGTCAACTGCAGATGTGCCAGAAGTTCCTGATTAGCAGCAATATCGAACACATTGATTCC
TCGCAATGCCTGCACTTCGACAACCTCCCGAACACCAAAGAATTTGTAGAGGGAATGGAG
AATATAGTTGCAAGTGAGTATTACGCTATACCTCAACGGGGACTCTCGCGTCTAAAGAAG
GTTAGCGACAAGGTGAAGACGCAACTCTCCTTCGTGGTACCACCCATGATGCGGGACTTA
ACGAAGGGCAGGACAATCTTCCGAGAACATGCGACGAATGTGCGCAACATTGTGGAGGGA
GTACTAAGTGATATCCATATAAAAACTCTGCACTCGACTAAGTCATTCGAGGACGTCTAC
GAGCGGTTTGGCCATGATAGAAACGTTGTCAGCCTGATTGTGTGCTTGCTTATTCTTCTG
GTACTTTTTATTTTGATCTTTGCTCTACTGTGCGGTTGCTTTGGGCGCCGACGAACTGGA
TATGGTGATGAATGCTGCAGCAAGTCCACCGGAGCCACCTGCCTGCTTCTAGCCATACTG
CTGATATTCTGTGTTTTCTCCTTCATCGCACTGGTTGGACTTTTCTACTTTATGCTGGGT
ATGGTAACCTACCAGGGTGCCTGTGCACCACTGAGAGATCAGGAGAACAACACATTGTTC
CGGCAACTGGACGCGTCTATAGATCTGAATCACTATTTGCCGCCATCTGAAAGTAACAAG
GAAGTGGTGCAACCACTAAAAATGTCGAGTGCAATCAAGGCTTGCCACGCCAATCAGACC
ATTTTCGATATGATGCGTCAGCATAACATATATGACATCAACGATCTGACGCGCATAAAA
GTGATGTCACACTCACAGGAAAATACAGATTCGATTAAGGTCTTCGACGAGGACCTGTCC
ACCGTGGTACTCCTGACGAAGGAAGAACGTGACGAGTTAAAGACAGCGGGCGAGAGCAAG
CTTGCGAAGTACCATAGCTCCCTGTACATGCCAAGTCTGTGCACCCAGTTTACGCCCATG
AACCTGAACGCCCTGTCCGAGCAGCTGTATAAGCTATCCAACGATCTGGAGTATCCGGCA
TACGGCTGGGCCAAGGTGTCATTCTGGAATGAGGGCCTCAACACCAAGGCCTTCTATCGC
AATTTTGTACCGAAGCTTACGAGCCTCGTAGAGAAGATGAAGGCCAATCTAAAGAAGATC
GACGAGTTAATTTCGTACGAGAACCACGACTTTACCAATACAATCAAAATTCTCACTGCC
ACGGCCATCAACTCCGAACAGTTCATCCAAACACGCGGCAAGGACTATATCAACGCCCTA
GGAGGCAATCTGACGAATTCCATTGACCAGATGATCGACGACTACATAGATATGATTATA
AAGGAGGCCAACGAAAGTGTGGGTCATTGTGCACCCCTGTCGTACATATATTATCGTGGA
GTCGATTTGATTTGCCACCGTATCGTGGATCCCATCAATGGATTCTGGGTAGGCATACTG
CTGTGCGCCCTGCTCTTCCTGCCCATCCTGTTCGTGGCCCACCGCCTGATGTGCCTCTAC
AAGAAAATCTATCCTTACCTGGCTACCGTAGGAGCCGCTGGAGTGGTCGAGGGCGGAAGC
GATTATCTGTACGATGCTTATAGTGAACGAGATCGTGAACATGTACCATTGGCTAACGTT
CCCAAGAAGCGTCGCAAGGCCTACGAGCGGCGACGGGAGCAGCAGGATTACTTCGAGGAC
GCCTCGCCCAGCGTTTCGCGTGGCAATCGCTCTGGCGGAGATCGTGGTGGCGGCGGAGGT
GATGGAGCTCCTGGCAGCAGCAGCATGCGTTACAACGACATGGCGCCAACGCATTGGGAC
CACGAGCCACCACGATACCACAATCCCCCAGCTGCGCCGCCATCATCGGAGTACGAGCGT
CCGCCGCCCTACTACTATCCGGGTGCCTCCGAGCAGGAT
Protein sequence predicted based on nucleotide sequence
MGEVAATVEPLAAVKSKSMRSRKRRQRRRRQIAYLAICGLSVAIFGFALATLIRPTTAQA DADPGSWRKGYVGHGTTHEQLGQPHWPPVQYTVYRPTTNYTKAPPPPTSAMNPIFNFTHF LYDKVLYRDEPIPEGYIVVKNSDTLSLGPKVEENDWRDLLAHYWMVLIWVVILVVLIIVI PFIAVCYCCFCCCRRCRQGCPPCTSKQDAQRRFCCGICLLILIIGLIFGIIIAFVTNKMI DSGFAETSETMKRGSEDTCTYLKDVADHVHHLMMYNYEEMETHVLDQLTHAHRHIFLDLS DTSESNSLAEMERVLENMPEALELMRQVEKMEKDLRFYGSQLRDGVRGIKRDVNFAVANL CQLQMCQKFLISSNIEHIDSSQCLHFDNLPNTKEFVEGMENIVASEYYAIPQRGLSRLKK VSDKVKTQLSFVVPPMMRDLTKGRTIFREHATNVRNIVEGVLSDIHIKTLHSTKSFEDVY ERFGHDRNVVSLIVCLLILLVLFILIFALLCGCFGRRRTGYGDECCSKSTGATCLLLAIL LIFCVFSFIALVGLFYFMLGMVTYQGACAPLRDQENNTLFRQLDASIDLNHYLPPSESNK EVVQPLKMSSAIKACHANQTIFDMMRQHNIYDINDLTRIKVMSHSQENTDSIKVFDEDLS TVVLLTKEERDELKTAGESKLAKYHSSLYMPSLCTQFTPMNLNALSEQLYKLSNDLEYPA YGWAKVSFWNEGLNTKAFYRNFVPKLTSLVEKMKANLKKIDELISYENHDFTNTIKILTA TAINSEQFIQTRGKDYINALGGNLTNSIDQMIDDYIDMIIKEANESVGHCAPLSYIYYRG VDLICHRIVDPINGFWVGILLCALLFLPILFVAHRLMCLYKKIYPYLATVGAAGVVEGGS DYLYDAYSERDREHVPLANVPKKRRKAYERRREQQDYFEDASPSVSRGNRSGGDRGGGGG DGAPGSSSMRYNDMAPTHWDHEPPRYHNPPAAPPSSEYERPPPYYYPGASEQD
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
