Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
8630
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00765127
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2913nts
Open reading frame: 1 to 2913
ATGCTGAAAATGGCAATGGCAAATGGTGCAAATCCGCTGCAAACAATGAAGAAAGCAAAC
GGCGACGAGGAGTCCGATGAGTCCGATATGGAAGAAGAGGAGGAGGATGAGGAAGACGAG
GAGGAGGACGATCTGCCGGATGTAGCACTCCAAATCGACAGCGAGGATGAGGCGGAACTG
GAGGCGCAGCGCATTCGTTTGGTGGAAGAAGAAGCAGCCAAGACAACAAAAAAGGCCGAG
AAAGAACGGCAGCAGCTGCAACAAAAACAACAACGGCAAGCGCAGGTCAGTAACGGTAAC
GAAGAGCAGCTGGAGGAAACTCAAAAACCCAGCAACAACAAACGCAAGGCCCGTGGGAGG
CCAAAACGCAAGATATCCACTAGCTCGCAGGCCTCCAGTGCCTCCATCGCCTCCAGTGCT
ACCTTGGTGGCCGCTCAAGTTCTCGCAGATCAGAAGTCACGCCGCTTCATCTACAGCTGT
ACCAGCTGCACACACCACTACGACGACAACCTGATCCTGTCCAGACACTTCCAGCTGGAG
CATGTCGAGAAATCGAAAAGGAAGCGAGGCAAAACCAAGGCTGCTGCTGAAGAACCCATG
GTTACTCCTCCCTTGATCATTGATCCCTTGGAAATGGGCCAACTTAAGCCCTGCGCCCAG
TTGCTTGTCCAAGGAGAATCCGTGCTGCAATTATGCCTCGCCTGTAATTCACAGTTTGTG
GATGTGGTACGCTTTCAGGAGCATCTTAGCCAGCAGCATGGCTATTTTCAGTTGCTGGTG
CCCAAGGAGGAACCCACCGATCTCCCACCGCATGCCATGGTCACAATTCCCGGGGCTCTG
GCTCTGAACAAGGCCAATGCCCAGCTTCAGCTGCCCATTCCAGCTCCGGAAACTCCGCCA
CCCGAGCAGGACGGCAAGGAGGCTCCACCAAGTAACGCGTCCAATGACAAGGAGGATCTC
ACCATGATCAACGACATGGTGGCGGTTATTGCAGCCGAGCGAGTGAAGAAAACCAGCAGC
TCCAAGGTCAACAAGATGATCATCAAACTTCCTATGCCCAAAAAAAGAGGCAGAAAGCGC
ACCAAGCCGGAGAGCCTGCGAGACGAACAGGATAATGCGGAGAAGACGGACAAGAAGAAA
AAACTGAACAAAGAGGAGGGACAGCAGCCAGTATTGGAGCCACTGCAGGCCAAAAAAACA
AGCAACCAGGCGGAGAACGGTGCAACGCAGTCAAATAATGTCGTAAAAGTTCAACAGAAC
GCAAAGGGATCAAAAAAAGACGGTACTCAGTCGGCCCAGAAATCTGAGACTAATCCCAAG
CACCTAGAGAATGCTGAGACAAATTCAAAGGATAATACAGAATCCGTGGCTCTGAAAGAA
ACCATCCACGATAAGCAGTTGACAATCGATGAAGCAGGCAAAGCAGGGACACAAAAAGAT
GACAACAGCAACCAGAAGCAGTTGGACAAGAGTGAGGCGAATGCTGGCAAATCTAAAATG
GTGGATCAAGTGCATCAGCAAATGCCTGTCGAGAGTGCAAAGGATCACCAGCAGATGACA
GAGGAACCCACCAATTCAACCAAGCGCAAGCGCGGACGCAAAGAGCGTAAGTCACCGACA
CCCGTACTCATTCCGCTGGGAGACAAAGAAATCAAGGAGGAGCCAGAACTCCCAGAGCGG
CGCATGCGCAAGAGCCGCCAGCATGTGGACTACGTTGTCGAAATTAAAGATGAGGAGGAG
GATGATGACGAGGTGGAAGAGGCTGAGGTGGATGATGACTCCTCAGCCACTATTTCGCCG
CACAACAGCTCAACAGATGAGAGTTTTACCTCGATTAAACGTGAGTCATCGGAGGAATCG
CAGCACAACGGAATCGGGGGAATTCACACCTGTAATTTCTGTGGCAAAACCTTTAAGCGC
TTCTCAAGAATGCAAGATCACCTGCGCCTCCATACCGGCGAGAAACCATACGTGTGTGGA
CAGTGCGGCAGGGCTTTCCGTCTAAAGATGCGTCTGGTGGAGCATCAATTGCGTCATCGC
GCCGAGAAGGCATACAAATGCGATATCTGTTCAATGCCACTGGCCACCAAGCAGGATCTC
TCGCTGCACATGCGTCATCACAAGAACGATCGGCGGTACAAGTGCGACAAGTGCAACAAA
GGATTTGTTCGCAGCTCAGATTTGTCCATCCACGTGCGGATTCACACGGGTGAAAAGCCA
TACAGCTGCGATCTGTGTGGTAAGGCGTTCCGTGCACGCCAAAATCTGGTGGTCCATCGA
CGCACTCATCTCGGCGATAAACCCATTCAGTGCGAGCTGTGCGACAAGCGTTTCGCCCGC
AAGATAGATATGCGGGTGCACATGCGCAGGCACACAGGTGAAAAACCCTACAACTGCGAT
GCCTGTCAGCGTGGCTACTCGTCGCGTGTAAATCTATTGCGCCACCAGGAGCGGGAGCAT
GGCATCGAGGAGCAAGTCCCTGGATCTGGCCAGGCGGATAAGAAGCACACAAAAACGGCA
ACAGAAAACGAGCAAGAAAACGAACCCAAAGCCAAAGCAGCGCCACGACGTCCGCGACGC
GAGAAGCTGCAGCAAAAGATTGCTGCTCAGGAGAAGCTACTGAATGACCTGAAGCGTAGC
TTGAGTGCTATGCCAGAAATACCTGAGGAGTCAGCGCAGGTCAAACTTGATGGATCTGGC
CAGGACATACCGGCAGATGCCGGAGCAGGACATGCTCAAGTCCAAGTCACGCTGTCCATG
CAAATGGAGCCAGCACCCAATACCGAAGACGACGAGGAGATCACGCCGGAGAAGGAGAAC
GCTTTGTCCAGCTCGATGACAGCCGATGGCAAGACAAAGGCGAATCGCAAGATAACCAGC
TACTTCACTGTCGTGGGTCAGCAGGCGGAAATT
Protein sequence predicted based on nucleotide sequence
MLKMAMANGANPLQTMKKANGDEESDESDMEEEEEDEEDEEEDDLPDVALQIDSEDEAEL EAQRIRLVEEEAAKTTKKAEKERQQLQQKQQRQAQVSNGNEEQLEETQKPSNNKRKARGR PKRKISTSSQASSASIASSATLVAAQVLADQKSRRFIYSCTSCTHHYDDNLILSRHFQLE HVEKSKRKRGKTKAAAEEPMVTPPLIIDPLEMGQLKPCAQLLVQGESVLQLCLACNSQFV DVVRFQEHLSQQHGYFQLLVPKEEPTDLPPHAMVTIPGALALNKANAQLQLPIPAPETPP PEQDGKEAPPSNASNDKEDLTMINDMVAVIAAERVKKTSSSKVNKMIIKLPMPKKRGRKR TKPESLRDEQDNAEKTDKKKKLNKEEGQQPVLEPLQAKKTSNQAENGATQSNNVVKVQQN AKGSKKDGTQSAQKSETNPKHLENAETNSKDNTESVALKETIHDKQLTIDEAGKAGTQKD DNSNQKQLDKSEANAGKSKMVDQVHQQMPVESAKDHQQMTEEPTNSTKRKRGRKERKSPT PVLIPLGDKEIKEEPELPERRMRKSRQHVDYVVEIKDEEEDDDEVEEAEVDDDSSATISP HNSSTDESFTSIKRESSEESQHNGIGGIHTCNFCGKTFKRFSRMQDHLRLHTGEKPYVCG QCGRAFRLKMRLVEHQLRHRAEKAYKCDICSMPLATKQDLSLHMRHHKNDRRYKCDKCNK GFVRSSDLSIHVRIHTGEKPYSCDLCGKAFRARQNLVVHRRTHLGDKPIQCELCDKRFAR KIDMRVHMRRHTGEKPYNCDACQRGYSSRVNLLRHQEREHGIEEQVPGSGQADKKHTKTA TENEQENEPKAKAAPRRPRREKLQQKIAAQEKLLNDLKRSLSAMPEIPEESAQVKLDGSG QDIPADAGAGHAQVQVTLSMQMEPAPNTEDDEEITPEKENALSSSMTADGKTKANRKITS YFTVVGQQAEI
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
