Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
522
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00765472
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6024nts
Open reading frame: 1 to 6024
ATGTATTTGACAGCCTTTCCCTTTTGGCCATTGGTATGCTTCGTGTGGCTCTGTCTCGGT
CTCGGCGACTTGACAAAAACTGCTGTGAAACGTAACGCGCCCCACTATGGAATTCACCCG
ACAAACGCACTGGGTCGTTGGGATTGGAATAAGCACAAGCAGCTGCACTCGGATGCCAGA
ATCGGACATTTAGAGGCGGGCAACGAGTCGCGGGAGGATCAGCTGTCGGAGGAGCTGAGC
AAGTTCGGCGAGAAACTCTTTCCCGTCTCCAACTATCCGCCGTATCAGCAGAATAACGAG
GATAGCCAGGAGGTGCATCATAGTGGAGAGGGAAGATTGCATACGATGGAATCGTCTGTG
CCATCCAAGACTAGAATATCCGGTGGCACAAATTCAAACGATACTCCCAAGTTCCGGAAC
ATTGGCGCCCAGAGGGGTAGAACCGAAATAGCCGGCATTCGCGAAACTGATCGAATCAGT
TCACGTGGTCGAGATGGTTTTATAGGTGGTCCGAGTAACAAACAGAAGCCATTTAGAGTC
AATCTGGCCGTTCTGGTGCAGGACGATCCCAGTCAACCGGGTGAGACATACTCCAATTGC
CAGAACTCCAATGAATCCGACGAGAACTGCAATCCCGAGGAGGATGCAGTGCCGAATTTG
CAAAGGGATTGCGAGAAGACCGGGATACATGTGATGTGCCCGCGAACCTTTCGATGCATT
AGCAAGTATTGGCTCTGCGATGGAGATGATGACTGCGGTGACTACTCGGATGAGACGCAC
TGCGGAGCCAGGACGAATTGTACAGATGATCAGTTCGAGTGTCTCAACGGATTCTGTATT
CCACGAACCTGGGTATGCGATGGCGAGAACGATTGCAAGGACTTCTCCGACGAGACCCAC
TGCAATCGCACCACCTGCACGGATGAGCACTTCACCTGCAACGATGGCTATTGCATCTCC
CTGGCCTTTCGTTGCGATGGTGAGCACGATTGCAATGACAACTCGGATGAGCTCAAGTGC
GCGGCGGTCATAAACAGCTGTCCGGAGGGCGAGTTCAAGTGCCGCGGCGGTTTGGGTGGA
GCAGGTGGTCCCAGTGGCCAGTGCATTCTCAATCGTTTCCGCTGCGATGGCGACAACGAC
TGCGGCGACTGGAGTGACGAGGAGAATTGCCCCCAGAAGCCATCACTATGTACCTCGAAT
GAATACAAGTGTGCCGATGGCACCTGTATTCCCAAACGTTGGAAGTGTGACAAGGAGCAG
GATTGCGACGGCGGAGAAGACGAGAACGACTGCGGTAGTCTCGGCTCGGAGCATCCCCTG
ACCTGCGGATCCGATGAGTTTACCTGCAACAATGGGCGCTGCATTTTGAAAACTTGGCTC
TGCGATGGCTATCCAGACTGCGCTGCTGGAGAGGATGAGGTTGAGTGCCACCTACAATGC
GACCTGGGACAATTTTTATGCCCAACCAAACAAAACCTAACAAATCTTAAGATATGTGTC
CATCAGAAGCACATTTGCGATGGCCACAACGAATGCCCCGCCGGCGAGGACGAGGCCGAC
TGTCCCAAGGAACGAAAGTGCTCGGAACCGAGTCCTTGCGAGCAACTCTGCATTGAAACC
ACTGCAGGATCGAACGAGTGTGCCTGTCGACTTGGATATGTCATGGACAAAAACAAAGTC
AACTGCACGGATATAGATGAATGCCAGTACCTGACCAGTCCGGTTTGCTCGCAAAAATGT
CACAACACTATGGGTTCCTTTAAGTGCTCCTGCGAAACGGGCTACATACTTCGTCCAGAT
CTCAGATCCTGTAAAGCGCTGGGTGGTGCGATGACCCTGCTGGTGGCCAACCGATGGGAC
ATACGACGGGTGACGTTGAGTAATAACCGATACTCGGCGATTGTTAAGGGACTTCACAAT
GCGATCGCATTGGACTTCCACCATCGCAAGGGCCTGATGTTTTGGTCCGACGTGTCCACC
GATGTCATCAAGATGGTGTATATGAATGGAACGCGAGTTCGAGACGTCATCAAATGGGGT
CTGGAGTCGCCGGGTGGCATTGCAGTCGATTGGATACACGACCTGCTATTTTGGACGGAT
TCGGGCACGAGACGTGTGGAGGTTTCCAATTTCCAGGGAAACCTGCGCACCGTCATCGCA
TCCTACGATCTGGACAAGCCACGTGCCATTGTGGTGCATCCGGGCGAGGCGTTGGCCTTC
TGGAGTGACTGGGGACCGAATCCGAAGATAGAGCGCGCTTACATGGATGGAACACAGCGA
AAAGTAATCATATCAAAGGGTGTAACCTGGCCAAATGGCCTGGCAATTGACTTCCCCAAC
AGCAAGATCTACTGGGCTGATGCCAAACAGCATGCCATCGAATGCTCCAATTTGGATGGC
AGTGATCGAAACAAGATCCTTAGCACCCATTTACCACACCCATTTGCTCTCACTCTCTTC
GAGGACACCATGTACTGGACGGACTGGAATACGAAAACTGTGTCGGCGGCGGACAAGATA
ACAGGGAAGGAATTCAGAGCGGTTCATGAGAATTTCCATTTCCCAATGGACATCCATGCC
TATCATCCGGCTCGACAGCCGGAATATGCTGATCGTTGCCAAAAGGATCGGCGGGGTCTG
CGCGGTGGGTGTTCCCATCTCTGTTTGCCGAACAAGACATCACGACGATGCGGATGTCCC
ATTGGACTTTCGCTGAAGGAGGACGGCAAGACGTGTAAGAGTACGGCCGATAAACTGGTG
TTGGTGGCGCGACGAAAGGACATTCGCTTGCGTCATCTGAGGGACAATCAAGCGGATCCC
AACGATGTTGATATGATTGTACCATTGGATAATCTGAAACATGCCGTTGCCCTGGATTGG
TGCAGCGACACGGACTTCATCTATTGGACAGACGTGGAGCGCAGCACCATCAATAAGGCG
CATCTCAATGGAAGCTACCAACAGCGAGTGGTGCACTCGAATCTTGTTTCCCCCGTGGGT
CTGGCCCTGGACTGGATAACCGATAAGCTCTATTGGACAGATCCGTCGACGAATCGCATT
GAGGTCGCAACTACGAATGGAAAGATGCGAACCTTGCTCATATGGGAGAAGCTGTACAAG
CCACGGGATATTGTTGTAAACCCGATTGAGGGCTTTATGTTTTGGTCGGATTGGGGTGAT
GATCCTATGATTGAACGTGCCAATATGGATGGACATGAACGTGTAACCATAACATCGAAG
AAATTAATATATCCCAATGGCCTGGCGATTGATTATGAGAAGAGCAAGATCTATTTTGTC
GATGGTGGCACAAAGACGTTGGAGAACATGAATTTCGATGGCAGCGGTCGCCAAGTGATT
CTAAATGGACTTGGCCATCCCTTCGGCTTGGATGTCAACGAGGGCCGAGTGTTTTGGACA
GATTGGGATACCAAGTCGGTGATGAGCGCCAATAAGCTGACTGGCAAGGACACCAATGTC
ATCATCGCCAACAGTACAGACCTGATGGACATCCGGGTGTTCCATCGCACCCGCAGGCGC
ATCTTCAATGCGTGCGACAAGCTGAACGGAGGATGCTCCCATCTGTGTCTCTTGAATCCC
ACCAGCTACACTTGCGCCTGCACTGTGGGTGTTCAACTGAAGGAGGATAGGCACACATGC
TCCGAAGGACCAACTCAGTACATATTGTTCGCTCATAGGATCGATATAAGGCAAATATCA
TTGGATTTCGATCATCTGATCGATGTGGTGCTACCACTACCGCCAATCTCGAATGCGGTG
GCTCTGGATGTTGATCGTAAGACGGGCTACATCTATTGGTCGGACACCATTGAGAACGTG
ATCATGAGCTCGAGTCCAGATGGTCTGCACGTTCAGAAAATCGTCGGCGATAGCCTGGAG
AATCCCGATGGTCTGGTTGTCGATTCGATTGGTCGTACTATCTACTGGGCCGATGCCGGT
CGCCATACCATCGAAGTTGCAAGTCTTGATGGTAGCAATAGGCATGTGATTGCCTACAAG
GATCTGGAGTCGCCACGTGGCTTGGCCTTGGATTACGAGGCTGGCTTGTTGTTTTGGACC
GACTGGGGACACTATCGCAAGATTGAGAGATCCCATTTGGATGGCAACGAGAGGTCTCGC
ATTGTTACCGCCAATCTGGGATGGCCCAATGGTCTGTCACTCGATTTGAAGAGCAAACGA
ATCTACTGGGTCGATGCCCGCCTGAAGACCATCGATTCTTGCGATTATACTGGAAATCAA
CGCAAGTTGATAATGTCATCTTTGCATCATCCGTATGCTCTAGCTCTATCGGATGATAAT
ATCTACTGGACCGACTGGAAGTCGAAGGCATTGCACATGACGGAAAGACGTAACATCAGT
GCAAAGCGGGATATAATCACCAACATCGATGGCCTGATGGACATTAAGATTATCTATCAG
AATCAGAATCAATCAACGATGAAAAATGCGTGTGGTAACAACAACGGCAACTGTTCGCAT
CTCTGTCTACGTAATCCATCTGGATATTCATGTCAATGCCCCATTGGATTGCGATTGCGC
CAGAATAGCACAACCCAGTGTCAAAATCTACCAGAGGATTATCTTCTTATTGCCTTGCGT
TCGGGAATTGGTATGATTTCGTTAAACTCTGGAGATTTCATGGACGTCGTTTTGCCCATA
AACGGAGTGCATGGAGCAGTCGTACTCGACTATCATTACCGCAAGAATCTACTCTTCTTT
GCCGATGTTAATTTGGATGTTATTCGTAGAGTTAACCTATTGAACTTAACCGAAAGCAAG
GTGATTGTCGGTACGGATGTGCTTACGCCCAACGGAATAGCCGTCGACTGGATTGCGGAT
AATCTGTATTGGTCCGACACTGATCGCAAACTAATCGAGGTATCTCGACTCGATGGAAGC
TGTCGCAAGAGGATCGTCGAGGATAACTTGGGTGACCCACGATCGCTTATTGTGCATCCA
AAGAAAGCCTACCTGTTCTGGTCGGATTGGAGCTCGCCGGCGAAAATCGAACGTAGCTAC
TTGGATGGCTCAAATCGCACGGTGATCATAACATCTGGAATCGGCTTTCCAACGGGCCTT
ACCATAGATTTCACCAATCGCCGCTTGCTGTGGGCCGACGCCCTGGAGGACAATATCGGT
CAAGTTGATTTCAATGGTAAACGTCGTCAGACCATTGTTCCTTATGCACCGCATCCTTTT
GGTCTGACTTTGTTTGAAAATAGTATATTCTGGACGGACTGGTACAACAAGTCGGTGTAT
CGATCTCAGAAACTGGCGCGCAGTGGATATGGCAATCCGTTTGAGGTGCGCGATGCCCTA
AGTGGAGCACTCGATATTCGCGCTGTCTCGCTGAGCCGTCAACCAAAAAGTGTGAATCAC
TGTGCCCAGGATAATGGTGGCTGTACGCATCTATGTCTTAATCGCAATGTGGACTATGTG
TGTGCCTGTCCGGATGTGATGGATCCCAAGGATGTGGCATGCTCCATTAAGCCAAAAGTT
TTGGTGCACGCCAACTTGGAGAACGATCAGTTGCTGGAATATTCCGACGAATCCACCGAT
GATTCCACACCGCCTGAGCTGCTCGGCGAGTCCAATGATCATGGTGATGATGGTCATACG
AAGAAGAGCGATCAGGAGCGAGAATTCTTTGTCCTCGTGGCGCTGGGCGTGGTCATTGTT
ATGGTGGTCATCATAATACTTGTTATTTTCATGCTTGCTTTCAACACGAGCAAGAAGAAA
AACAAACGCAATTCCCGCGGCTCCAGTAGATCTGTACTAACCTTCTCAAATCCAAACTAC
AACGTTGATGGCACGCCAATGGAACCGAAGACGAATATATGGAAACGTTTTAAATACGAT
AAAACCCATGAACGAGCCGGGGTCTATGAAGAGCGCAGTCTAACCACGGAGACGGCATCC
TCCAGCCTCTTCGTTCCGACTCCATCCCCGATGGCGTCGCCCTCAACAAAAACAATCCAA
CTGACAACGCTCTCGACTATCACA
Protein sequence predicted based on nucleotide sequence
MYLTAFPFWPLVCFVWLCLGLGDLTKTAVKRNAPHYGIHPTNALGRWDWNKHKQLHSDAR IGHLEAGNESREDQLSEELSKFGEKLFPVSNYPPYQQNNEDSQEVHHSGEGRLHTMESSV PSKTRISGGTNSNDTPKFRNIGAQRGRTEIAGIRETDRISSRGRDGFIGGPSNKQKPFRV NLAVLVQDDPSQPGETYSNCQNSNESDENCNPEEDAVPNLQRDCEKTGIHVMCPRTFRCI SKYWLCDGDDDCGDYSDETHCGARTNCTDDQFECLNGFCIPRTWVCDGENDCKDFSDETH CNRTTCTDEHFTCNDGYCISLAFRCDGEHDCNDNSDELKCAAVINSCPEGEFKCRGGLGG AGGPSGQCILNRFRCDGDNDCGDWSDEENCPQKPSLCTSNEYKCADGTCIPKRWKCDKEQ DCDGGEDENDCGSLGSEHPLTCGSDEFTCNNGRCILKTWLCDGYPDCAAGEDEVECHLQC DLGQFLCPTKQNLTNLKICVHQKHICDGHNECPAGEDEADCPKERKCSEPSPCEQLCIET TAGSNECACRLGYVMDKNKVNCTDIDECQYLTSPVCSQKCHNTMGSFKCSCETGYILRPD LRSCKALGGAMTLLVANRWDIRRVTLSNNRYSAIVKGLHNAIALDFHHRKGLMFWSDVST DVIKMVYMNGTRVRDVIKWGLESPGGIAVDWIHDLLFWTDSGTRRVEVSNFQGNLRTVIA SYDLDKPRAIVVHPGEALAFWSDWGPNPKIERAYMDGTQRKVIISKGVTWPNGLAIDFPN SKIYWADAKQHAIECSNLDGSDRNKILSTHLPHPFALTLFEDTMYWTDWNTKTVSAADKI TGKEFRAVHENFHFPMDIHAYHPARQPEYADRCQKDRRGLRGGCSHLCLPNKTSRRCGCP IGLSLKEDGKTCKSTADKLVLVARRKDIRLRHLRDNQADPNDVDMIVPLDNLKHAVALDW CSDTDFIYWTDVERSTINKAHLNGSYQQRVVHSNLVSPVGLALDWITDKLYWTDPSTNRI EVATTNGKMRTLLIWEKLYKPRDIVVNPIEGFMFWSDWGDDPMIERANMDGHERVTITSK KLIYPNGLAIDYEKSKIYFVDGGTKTLENMNFDGSGRQVILNGLGHPFGLDVNEGRVFWT DWDTKSVMSANKLTGKDTNVIIANSTDLMDIRVFHRTRRRIFNACDKLNGGCSHLCLLNP TSYTCACTVGVQLKEDRHTCSEGPTQYILFAHRIDIRQISLDFDHLIDVVLPLPPISNAV ALDVDRKTGYIYWSDTIENVIMSSSPDGLHVQKIVGDSLENPDGLVVDSIGRTIYWADAG RHTIEVASLDGSNRHVIAYKDLESPRGLALDYEAGLLFWTDWGHYRKIERSHLDGNERSR IVTANLGWPNGLSLDLKSKRIYWVDARLKTIDSCDYTGNQRKLIMSSLHHPYALALSDDN IYWTDWKSKALHMTERRNISAKRDIITNIDGLMDIKIIYQNQNQSTMKNACGNNNGNCSH LCLRNPSGYSCQCPIGLRLRQNSTTQCQNLPEDYLLIALRSGIGMISLNSGDFMDVVLPI NGVHGAVVLDYHYRKNLLFFADVNLDVIRRVNLLNLTESKVIVGTDVLTPNGIAVDWIAD NLYWSDTDRKLIEVSRLDGSCRKRIVEDNLGDPRSLIVHPKKAYLFWSDWSSPAKIERSY LDGSNRTVIITSGIGFPTGLTIDFTNRRLLWADALEDNIGQVDFNGKRRQTIVPYAPHPF GLTLFENSIFWTDWYNKSVYRSQKLARSGYGNPFEVRDALSGALDIRAVSLSRQPKSVNH CAQDNGGCTHLCLNRNVDYVCACPDVMDPKDVACSIKPKVLVHANLENDQLLEYSDESTD DSTPPELLGESNDHGDDGHTKKSDQEREFFVLVALGVVIVMVVIIILVIFMLAFNTSKKK NKRNSRGSSRSVLTFSNPNYNVDGTPMEPKTNIWKRFKYDKTHERAGVYEERSLTTETAS SSLFVPTPSPMASPSTKTIQLTTLSTIT
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
