Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
4063
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00769575
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3417nts
Open reading frame: 1 to 3417
ATGTCTCGGATAAAGAAGAAGGCAGATGAACATGCCTCCAGCCGCCGCAGGACTCAGATC
AATCTGATCGAGTTCAGTTTCTTGGATCACAAGGCGGAGCTCGTGGGCCTGCTGCAGGCC
AAGAGCCGCCAGGTAGGCAGGAGTCTGGTGGAGAACGAGGCTGACTTCTGGAAGTTCGTG
GGCAAGTACGAGGCTATGATGCGCTCCACGGGCCAGCCCGTGCTGCCGCCTCGACTCGCG
GAGGCGGAGCTGAGCAGCATGCAGCCGTATCACCGCAGCAAGCACTTGGCATTGCGGCTG
GACGCTGACGAACTGCTTCAGGAGGCGCGCGACCAAATGCAGTCCCAGTTTCGTCATATC
TTGCTGGTGTACTTAGACTTCCGCCAGACGGAAAAGTTCCAGCGTATCCGAAAGCTGCGG
CAGACACAACGCAATCTACCCATTGCTCGCTTCCGGAAGGACTTGCGCGAGGCACTGGAC
ACGTCCCGCGTGGTCATCGTGGCCGGTGACACTGGCTGCGGCAAGTCCACACAGGTGCCC
CAGTATCTCTATGACTTTGGCTACCGCAGCATTGCTTGCACCCAGCCGCGCCGTTTGGCC
TGCGTCTCCCTGTGCAAGCGCGTTGCGCACGAGCTGCTGGACGACTACGGCAGTCGGGTG
GCATTCCAGATCCGATTCGAGCGCAGTCGGACGAAGCTCACCAACATCCTTTTCATTACG
GAGGGCTTGTTGCTGCGGCAACTAGCGGTGGCCGCCAACCTAGACCAGTACGATGCCCTG
ATTTTGGACGAAATCCACGAGCGCAACTTATTCGGTGACTTTCTACTGGGTGTTACCAAG
TGCTTACTGAGGGCGCGGCCGCAACTGAAGCTGATACTCATGTCTGCCACCATCAACGTG
GAGCTCTTCCACGGCTACTTCGGCGAGGAGGGCGCACGGCTGGTCCAGGTGCCGGGACGT
TTATTTCCCATCAAACTGCGCTATCTGCCGCCTCCTGCACTGGAACTAAAGGCGGGACAG
GCGACATCCAAACGCTCTCAGAGAAACCGCATCGATCCAGCTCCATTTGTCCAGGTGCTT
AGCCTTATTGACCAGCAGTATCCGACCAGCGAGCGCGGTGACGTCCTTATCTTCGTTAGC
GGCGTAAACGAAATCGAATCTGTTGTGGAGGCCGTTCACGAGTATGCCACCGAACAGACT
CATTGGCTCGTCCTGCCGCTGCACAGTGGCCAGGCGATCGCGGACCAGAGCAAGGTGTTT
GACTACGCACCCGAGGGCATGCGCAAGTGCATTGTGTCCACCAACATAGCCGAAACGTCG
CTCACCGTGGACGGCGTGCGGTTCGTGGTCGACTCTGGCAAGGTCAAGGAGATGAACTTC
GACGCAACCTGCAAGGGCCAGCGTCTGAAAGAGTTCTGGGTGTCCAAGTCGTCTGCGGAT
CAGCGCAAGGGTCGTGCTGGTCGCACGGGACCAGGTGTCTGTTTTCGGCTCTACACTGCG
GAGCAATACAACGCCTTCGAGGCATATCCCACTCCGGAAATTTACCGCGTGCCGCTCGAC
ACGATGCTGCTGCAGATGGTGTCCATGGGATTGCCCGACGTCCGGGCCTTTCCATTCATA
GAGGCCCCGGAAACGGAGCGCATCGAGCAGACTATTCTGGCGCTAAAGCAACACTGCGCC
CTCAGCGTGGAGGAAAAGATCACACCGCTGGGCAGATCGCTGGCCAACTTGCCCGTAGAG
CTGTCCATCGGCAAGATGCTGCTTATGGGCAGCGTGTTTCCCGAGGTGGAGCAGCTGCTG
ACCCTGGCCGCCATGCTGAGCGTGCAGAACCCGCTGACGAATCGTGCCCACACAGACCAG
CGCTGCGTGCGCGAGCGGGAGTCTCTGGAGTCCGATCATGGCGACCTCTTCACCCTGGTG
CGACTCTACAACGTGTGGGTGCAGCTGAAGATGCGGCGCGATGGCACTCGACAGTGGTGC
CGTCGCTTGGGCATCGAGGAGCAGCGCTTCTATGAGGTGACCAAGCTGCGTCAACAGTTC
CAGCGCATCCTCGAGAGCTGCGGCATGGTGGTGGCCAGCGATTCGGACTCCCAGTTGACC
AGTGCCGAGCGCGCAACGCGACATGGTGAGCTGCGCCAGCTGAAAGCCATGAAGCGCCGC
CAGCGATTTGAGCAACCCCGTCAGCGCAAGTTGCTCAAGCAGAGCGCTGGACGGGTAGCG
GAGGACGAGGAGGAGCAGGAGGAGGCGCAGGGCGACGATATGAGGGACGTGGACTTCCGG
CTGCGGTTCGATCCTCGACAGTTGGCGCTGCTGGAGCGTTCGTCGCGGCTCGACCGACAC
AGCGTCGTTGTGCTGCTAAAGCTGCTGCTCGGCAGCGGTTTCTATCCACAGCTGGCCATC
AGCGACGAGTTCAACTACTGCAAGGGTGGTGGTCAGCAGTTCTTCCACACGCGGCTCAAG
CCCTTCGTGCTGCAGCACCCAAACTCGCAATTCGCCAAGCACTTCGAGCTGCTTAAGCTG
ACCGAAAGCGATCTGCTGCCCAAGCCAGACTTCTACACGCCCAAGCTTCCGCTCAGCAAG
CGCCACCAGTTGCTCTGTTACCAGTCGCTGCTGGAGACCGCCAAGCCCTATCTGATTAAC
TGCATCCGCCTGCCGGCTGCCCAGACGCTATTGCTCTTCAGCTTCGCCATTGACACGAAC
GCGGGCATCACACAGATCGCCTGCGACGGCTGGCTGGGACTGGATCTGCCCATGCCCGGC
AGCGGTATGGAGCTTTTAAGACGGGCCATCGAGTTGCGCCGACGTTGGAGCAGACTGCTT
TACGACAAGCTGGATGACCTCAAAAGCAAACAGGAATCACCTTCCCATTCAAGTGACGAT
AGGAGCAGCGCGCTGTGGCAAGATCTTGTGGACTATATGGCGCTGGATGTGGCGTATGCG
ATACGCCGACTGCTTCCCGCCGACATCAAGAGGCTCTACACTCATCAGGCACCTTCCGCG
CGACTCGCGGAGCTCAAGGAGAACCCCTTTGCCGTCGACTTTCCCATGACACCGAACGAG
GAAAAGGGCGGCCTCAACGTATCGGAGCACGTGGTGTACAGCTGCTTGGCCGAACAGCAG
TGGACGACGGCCATGGATGCGACTCTACGGGCAGAGCCATGGCAGTGTACGCGCTGCGAT
TTCGAGCTGGAGGAGTTCGATGTGCTGGAGCAGCTGGTGCACCGATCCAAATGCAAGCGT
CGCAAGGCGCAGGAAAGCCGCACGGCGAAAACAACGAGCACAGAGTCCTCAACGGAGGTA
GCCAGTAGCTCCAGTTCGCACTCCTCCGGCGGCTATTACTGCAACTCCTGCAAGAGGGAG
TTGCGACTGACAACGATCGACATTCTGCGCCACAAAAAGCAGTGTCGAAATAACAAG
Protein sequence predicted based on nucleotide sequence
MSRIKKKADEHASSRRRTQINLIEFSFLDHKAELVGLLQAKSRQVGRSLVENEADFWKFV GKYEAMMRSTGQPVLPPRLAEAELSSMQPYHRSKHLALRLDADELLQEARDQMQSQFRHI LLVYLDFRQTEKFQRIRKLRQTQRNLPIARFRKDLREALDTSRVVIVAGDTGCGKSTQVP QYLYDFGYRSIACTQPRRLACVSLCKRVAHELLDDYGSRVAFQIRFERSRTKLTNILFIT EGLLLRQLAVAANLDQYDALILDEIHERNLFGDFLLGVTKCLLRARPQLKLILMSATINV ELFHGYFGEEGARLVQVPGRLFPIKLRYLPPPALELKAGQATSKRSQRNRIDPAPFVQVL SLIDQQYPTSERGDVLIFVSGVNEIESVVEAVHEYATEQTHWLVLPLHSGQAIADQSKVF DYAPEGMRKCIVSTNIAETSLTVDGVRFVVDSGKVKEMNFDATCKGQRLKEFWVSKSSAD QRKGRAGRTGPGVCFRLYTAEQYNAFEAYPTPEIYRVPLDTMLLQMVSMGLPDVRAFPFI EAPETERIEQTILALKQHCALSVEEKITPLGRSLANLPVELSIGKMLLMGSVFPEVEQLL TLAAMLSVQNPLTNRAHTDQRCVRERESLESDHGDLFTLVRLYNVWVQLKMRRDGTRQWC RRLGIEEQRFYEVTKLRQQFQRILESCGMVVASDSDSQLTSAERATRHGELRQLKAMKRR QRFEQPRQRKLLKQSAGRVAEDEEEQEEAQGDDMRDVDFRLRFDPRQLALLERSSRLDRH SVVVLLKLLLGSGFYPQLAISDEFNYCKGGGQQFFHTRLKPFVLQHPNSQFAKHFELLKL TESDLLPKPDFYTPKLPLSKRHQLLCYQSLLETAKPYLINCIRLPAAQTLLLFSFAIDTN AGITQIACDGWLGLDLPMPGSGMELLRRAIELRRRWSRLLYDKLDDLKSKQESPSHSSDD RSSALWQDLVDYMALDVAYAIRRLLPADIKRLYTHQAPSARLAELKENPFAVDFPMTPNE EKGGLNVSEHVVYSCLAEQQWTTAMDATLRAEPWQCTRCDFELEEFDVLEQLVHRSKCKR RKAQESRTAKTTSTESSTEVASSSSSHSSGGYYCNSCKRELRLTTIDILRHKKQCRNNK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
