Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
4295
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00770044
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4713nts
Open reading frame: 1 to 4713
ATGTCAAACAGGCGAAGAGTGCGAACAATTGATTATCTGGCGCTGCAGCAGAGTTTGCGT
TTGCAAAAGACACCGGCAGCAACAACAAATGCAGAGGAGCAGGTGGCAAGGGAGGAGGAG
AATGGCAATGGGCATCATAGTACCGTTACAGCTGAAACGCCACCCACACCACCCATCCCG
CCCATTCCGCCCATACGATTACGACGCGAGCAGAGCGTCGAGGAGGATGTTGCAAGAGTT
GAGTACCGTGTGAAGCAGACGCCTTCGCGTCCAGTGCAGATGACTCGAAATCGGATTGGT
GCTTTGGAGGAGGACATGCCTCCCGAAGATGAGCTGGCGGCGCACTATGAGGCCTTCGGC
ACTACTCCGCCACGTACCAGGCTCAAAATCAAGAACCGCGACTGGGAACGCAAACAGAAG
GTGGTCAATGTGACAGCTTCCGCAGACTTACCGGCCAGTGTGGGTGGCACCCCCAAAAAG
ACCAGGACGGCCAGATCCCGCGTCCTGCGGCGCAACACTATGGACTGCGCCCTGCTCAAC
GAGATGTTTGTCAACGATGAGAGCAAGCGGACGGACAAGCGGCTGCAGTCACTCCTTCGC
GACTCGGAACGTGAGATGAAGAACTCGTTGGCCACAACGGCGGTGGCTGCTCCACGAGGT
AACAGCTTCCACGAGACAGCTCATCCGCTGGAGTCTCTGGACCAGATCATGCCGCTCAAT
TCGGAGGCAATGGCGCCGATTCCACTGCGAATTGCTTCCAAGGTGGTGGAGAGCTGCAAT
CGCTATCGCTGCGTGTCCTCACGTCCCATTGGGTATCGCTCCTCGGCGCCGCCGTTGGCT
TTCGCTGCCCAACTTCCCGCTGGAATGCTAAGGAGTGATGGAAGAGCAACACCTGGGTTG
GGGAAAAGAAAGGACTTCCACGAAACGTTTGCCAATCTAATTAAGCTCGGTAGCGTAGAT
CGACAGGACGCCAAACTATCACAGGAGGAGCACACCTGGCAGACTGAGCTGAAGGATCTC
ATCTGGCTAGAGCTACAGGCCTGGCAAGCAGACCGAACTGTGGAGCAGCAGGACAAGTAC
CTTTTCGAAGCGCGTCAGGGAGTCTCCGATCTGCTCACCCACATTATAAACTACAAGTTC
CAGCCGCGTTATCGCCGCGAGCCGAGTCTGATAAGCTTGGATAGTGGCACTCATTCCGAT
AGCAATTCTAATGCCAGCTCTCCACTGCCCAGCAAGATGTGCCAAGGATGCATGTCGCTG
TACTGCAAGGATTGCATGGATCATCAGGAGTTGGCGCTGCGAGAAGTGGAGGGTATGCTG
ACGCGCCTGGAAGCTGCAGAGGCACTCTACCCTTCCTCACAGGCCATGGGCGCCCTGCAT
CCCATTTACAAATCACAGAGCTTTGTAGGGCGCATCAAGTCCATGTGCTTATGGTATAAC
ATTACCAAGCAGAACAAGTTGAAGCTCAGTATTTTAGGAAAAATTCTGGCAAGACTGCAG
GATGAGAAGTTCTCTTGGCCCGTGTGCACTTCCTCCTACATCGCCACCGACAGCGGTAGT
TCATCAGCATCGGGTGTAGAGAACGATGATTCGGCTGTTAACTCAATGGATTCATCAAAG
CCGCCCAGTATGGCCGGATCAGCCTCGCGAAAGGGTGTGACACCGTGCCACAAAGTGCAA
TTCATGTTAAACGACGCCACCCACGTGCCAGGCGAGACATCAAGTAGCAATGAATCCACC
TCCACGGAGGTAAGCCAGTGGTCCAGCGAGTGCTCTCACAGCCATATGCGTAAGGGATCC
ATGCACGACATCAACATTTTCAGTGTCGAGCCATTGGGTACGTGCAGCAGCAATGGGACA
GGAGAGCAATCCACTGGGCTGTATCGCAAGTTCATTGAAAATGTGCTCAAAAGTCGAGGA
CTGGCCAAGTCGCTGGCTTTTCTACACAAGCTGCACAACGTGGCATTGTACAAGGCGCAC
ATAGCTCTGGAAAAACCCGGAGCTGAAGACTTCGATTACGAGTCGGATGCAGAGTCCATT
GAAGAGGACGTACCAAGGCTAGATCCTGAAATCAGCCGCGAACAGGTTGTGGAGCTGCGC
ACCTACGGCTACTGGAGCGAGGAGGCACAATCCATAAACCTGCCGTCGTACATTCCCACC
TTTGTATTTCTGAGCGGAATTCCGTTGCAGTTCATGCATGAGTTCTTGCGCATGCGTCTC
GAAACCAGACCCGTTCGACCCAATCCACTGAGCCTGGAACAGTTGATGAAGGAGCTTAGG
GAGGGCTTGACCCTAGCCTTGACCCATCGGGAGCGATACCAGCGGCACATAACCACGGCG
CTGGTGGAGAATGAGGCGGAACTGGGCAGTTATATTAGCATTTTAAACCACTACGATGCT
ACGGTGCGAAAGACTTTCGAGCTGTATTTGGAGTACATCGATCAACTAGTGCTGGTTGCC
GTTCCGGAAGGCAACCAGAAGTCCGTCCTTGAGAAGGAATGGATGTTTACCAAGCTTATC
AGTCCCATGATAAAGGGCATGCACACGTTAGCATCTCAAAAATTCTGTGGCATAATCAGC
AAACTTTTGCGGAGCATTTCCGAACGCCTGGTGAAGCGCACCGTTGAGCTAGATCAACAG
ATCGATGGCACCGCCGATACCGACGACAACGAGGAGGTTAAATGGCAGCTGCTAACCATT
TGCAGAGAGACACAATCGCTGCTCACAGTCGAACGGGAGCGCTCCATTAAGGTGCTCTTC
TTTGCCAAAACCTTTTGTCGCGACGTCGAGACGACGGATTTTCATCGCGAGCATTATGAA
CATGACGTGGCCAACCAGCAGCATGACTTCATCTGTTCGGACGTAAAGGCGGCGTTCAAG
CTCTTGCAGCAGGACGTTCTGCAAGTGCGCAACAAGTTGACGGCAATAATTGAAGGAGTG
CAAAAACGGTGCTGTTTGAGCAACATGCGAGATTTGGATGAGCAAGACAAACAGGCTGTG
CTGTCGCGCACTCGAGAGATTTTGCATCAGGGATACAAATTTGGATTTGAATATCACAAG
GACGTCATTCGGTTGTTCGAGCAGAAGATTATGGACCAAAAGGACAGCGGTGCTCACACA
GTAGATCTTGCCTTAGGAATTATCGCTTACGCTAAGATGTGGATGCATTTTGTAATGGAG
CGTTGCGAACGTGGACGAGGAATGCGTCCCCGTTGGGCCTCCCAGGGTCTGGAGTTTCTG
ATTCTTGCCTGTGATCCACAAATCACCCAGCACTTGGACGATGACGAGTTTGAGGCGCTA
AAGCAGCAAATGGATCGCTGTATTTCGCACGTGATTGGCATCACCTCGGAACCCGAAAAG
GTCGCCAAGAAAAAGGCTTCGCCGCGCACTCGCAAGACTTCATCGCCGGCCACCTCGCGT
TCCCGGACACCAACACGGACTCCAATGTCCGCTGGCATGGTCCTTAATCCGAATACGCCG
CCACTACAGTCGCCACCGTACAACAAGTTACTTCATCCGCAGTTCAGTTTAAAGGAGGAT
GTGTCAGGAACTTCGTACAGTCCCGTTGACAGTTCAGACTATGTTGACACTCCGTGCCAA
AGGAGTGCCAACGGCGAGCTGCGTCTGCTGGTGCCCCAGACGCCGCCGACTCCAGCATCT
CCCGGAAAGAGCAGCCTCGAAAGTACACCGCTGGCCTTGCGGCAGGAACGTGTTAGAGAT
GCCGTCAACCGTTTGGATATGGATCTAGAGGACGGGCTACGCGAACGAAGGTTGATTGGG
CAGGTCAAGTCTCTTAATTCCAGCGACAAGGTGCATATAAGGGCGCGTAGCGTTCATTTC
CGCTGGCATCGTGGCATTAAGATCGGCCAGGGACGATTCGGCAAGGTATACACGGCGGTG
AACAATAACACGGGAGAGCTGATGGCCATGAAAGAGATCGCAATCCAGCCGGGAGAGACG
CGAGCACTCAAGAATGTGGCCGAAGAGCTAAAGATCTTGGAAGGAATAAAGCACAAAAAC
CTGGTGCGTTACTATGGCATCGAGGTGCACCGCGAAGAGCTGCTTATTTTCATGGAGCTG
TGCTCTGAGGGCACCCTCGAGTCACTGGTGGAGCTGACTGGTAATCTGCCGGAGGCGCTT
ACGCGACGTTTCACCGCCCAGCTGTTGTCGGGCGTGTCTGAGCTGCACAAGCATGGCATT
GTGCATCGCGACATCAAGACTGCTAACATATTCCTCGTGGACGGCAGCAACAGCCTAAAA
CTGGGCGATTTCGGATCAGCAGTGAAGATCCAAGCGCACACCACTGTGCCCGGTGAGCTG
CAGGGCTATGTAGGCACGCAGGCCTATATGGCGCCGGAGGTTTTCACAAAGACCAACAGC
GATGGCCATGGCAGGGCGGCCGATATCTGGTCGGTGGGCTGTGTGGTTGTCGAGATGGCC
TCGGGCAAGCGTCCTTGGGCCCAGTTTGATTCCAACTTCCAGATCATGTTCAAAGTGGGC
ATGGGCGAGAAGCCGCAGGCACCGGAGAGCCTATCCCAGGAGGGTCACGACTTCATCGAT
CATTGTCTGCAGCACGATCCCAAGAGGCGTTTAACGGCAGTGGAACTGTTGGAGCACAAT
TTCTGCAAGTACGGTCGAGACGAGTGCAGCAGTGAGCAGTTGCAGATGCAGGTGCGAGGC
TCTTTCCGGCGAAATGTGGCGACCAGCAGCAGC
Protein sequence predicted based on nucleotide sequence
MSNRRRVRTIDYLALQQSLRLQKTPAATTNAEEQVAREEENGNGHHSTVTAETPPTPPIP PIPPIRLRREQSVEEDVARVEYRVKQTPSRPVQMTRNRIGALEEDMPPEDELAAHYEAFG TTPPRTRLKIKNRDWERKQKVVNVTASADLPASVGGTPKKTRTARSRVLRRNTMDCALLN EMFVNDESKRTDKRLQSLLRDSEREMKNSLATTAVAAPRGNSFHETAHPLESLDQIMPLN SEAMAPIPLRIASKVVESCNRYRCVSSRPIGYRSSAPPLAFAAQLPAGMLRSDGRATPGL GKRKDFHETFANLIKLGSVDRQDAKLSQEEHTWQTELKDLIWLELQAWQADRTVEQQDKY LFEARQGVSDLLTHIINYKFQPRYRREPSLISLDSGTHSDSNSNASSPLPSKMCQGCMSL YCKDCMDHQELALREVEGMLTRLEAAEALYPSSQAMGALHPIYKSQSFVGRIKSMCLWYN ITKQNKLKLSILGKILARLQDEKFSWPVCTSSYIATDSGSSSASGVENDDSAVNSMDSSK PPSMAGSASRKGVTPCHKVQFMLNDATHVPGETSSSNESTSTEVSQWSSECSHSHMRKGS MHDINIFSVEPLGTCSSNGTGEQSTGLYRKFIENVLKSRGLAKSLAFLHKLHNVALYKAH IALEKPGAEDFDYESDAESIEEDVPRLDPEISREQVVELRTYGYWSEEAQSINLPSYIPT FVFLSGIPLQFMHEFLRMRLETRPVRPNPLSLEQLMKELREGLTLALTHRERYQRHITTA LVENEAELGSYISILNHYDATVRKTFELYLEYIDQLVLVAVPEGNQKSVLEKEWMFTKLI SPMIKGMHTLASQKFCGIISKLLRSISERLVKRTVELDQQIDGTADTDDNEEVKWQLLTI CRETQSLLTVERERSIKVLFFAKTFCRDVETTDFHREHYEHDVANQQHDFICSDVKAAFK LLQQDVLQVRNKLTAIIEGVQKRCCLSNMRDLDEQDKQAVLSRTREILHQGYKFGFEYHK DVIRLFEQKIMDQKDSGAHTVDLALGIIAYAKMWMHFVMERCERGRGMRPRWASQGLEFL ILACDPQITQHLDDDEFEALKQQMDRCISHVIGITSEPEKVAKKKASPRTRKTSSPATSR SRTPTRTPMSAGMVLNPNTPPLQSPPYNKLLHPQFSLKEDVSGTSYSPVDSSDYVDTPCQ RSANGELRLLVPQTPPTPASPGKSSLESTPLALRQERVRDAVNRLDMDLEDGLRERRLIG QVKSLNSSDKVHIRARSVHFRWHRGIKIGQGRFGKVYTAVNNNTGELMAMKEIAIQPGET RALKNVAEELKILEGIKHKNLVRYYGIEVHREELLIFMELCSEGTLESLVELTGNLPEAL TRRFTAQLLSGVSELHKHGIVHRDIKTANIFLVDGSNSLKLGDFGSAVKIQAHTTVPGEL QGYVGTQAYMAPEVFTKTNSDGHGRAADIWSVGCVVVEMASGKRPWAQFDSNFQIMFKVG MGEKPQAPESLSQEGHDFIDHCLQHDPKRRLTAVELLEHNFCKYGRDECSSEQLQMQVRG SFRRNVATSSS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
