Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
4286
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00770072
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6696nts
Open reading frame: 1 to 6696
ATGGAAACTAGACAACCCAGTTCCGGTGATGATCTAAAGCCAGAGCTGTACTTCCTCATT
TCCAAGTTCCTGGCCGCGGGACCGCTGGAGGAGACGGCGAAGGTCTTGATCCGGGAACTC
GAAGAGAAGAAGGTTTTGCCGCGTCGTCTCGATTGGCAGGGAAACGAGCATGAGCAGACC
TTTGAGGAATTGGAACGAAAGTACAAGCACATTGGCGCCAATCACTTGCTTGAAATATGC
AGTCGTCTGGGTCCACTGGTGGATCGCGAGCTTCCGCCCAGTGTTCCGGGCATCAACTCG
CTGTTGGGTACTGGGCGCCAGAACCTCCTGCGCACCAAGGAAACCGTCTACTGCCATCGC
TCCTTAAGGGACTACTGCACCCGCCTGAATGGAGTCTCGCTGCCGGATTCGGTCCTTACA
AAGCCGACCCACAACTTAGATCGCGTTCTAACTGGACGGGAACACGGCGGGGAAGTCCGT
CGCAAGCTCCTCGTGCCCACGGACCTCTACCGCCGAACAAAATTGCTCCGTCGCACTGTG
GGCCACCTGTCCTCAGTGTACTGTGTTCTGTTTGATCGCACAGGAAGATACATCATCACC
GGAGCCGATGATCTACTTATTAAGATATGGTCAGCGGCAGATGGCCGCCTGCTGGCCACT
CTGCGAGGCGCCTCATCCGAGATTACAGACATAGCCATTAACTTGGACAATACGATGCTG
GCTGCCGGGTCATTGGATCACATACTAAGAGTGTGGGACATGCAAACCACATCACCCATC
GCCGTACTGTCTGCTCACACGGGCATGATAACTTCGGTTAACTTTTGTCCGTCGCCACGT
AGCGATCTGAAATATCTGGTGACTACCAGCACAGATGGATCGATCGCCTTCTGGCAATAC
TCAACGCCGCGCGGGCAAAAGATCACGTTTGCCCCGAAGCCAACGCAGTATCATGAGAAA
CTGCGGCCCGGACAAGCTCAGATGATGTGTACCACCTTTTCGCCAGGAGGCATTTTTCTG
GCCGCTGGGTCTGCCGATCACCATGTGCGCGTCTATATGATGGGCGAGGACGGTCCGAAG
CGAATATTGGAGACGGAAGCTTACACAGATGCCGTGGACTCGGTGCAGTGGTCGCATCGT
GGACTGAGATTTATATCGGGGAGCAAGGACGGCACAGCACACATCTGGACGTTTGAGTCG
CAGCAGTGGAAAAGTTCCAAACTATGCATGACGGAGCGTCTGGCCAGCTGCCCGGAACCA
GAGGAGGGAAAGAGGCTTAAGGTAACCATGGTGGCTTGGGATGCCTCGGACCGTTATGTC
ATCACCGCCGTAAACGATTTTACTATAAAAATCTGGGACTCGAAGTCAGCCAAGCTGCAT
CGTGTTCTGCGTGGGCACAAGGATGAGCTTTATGTGCTTGAGTCGAACCCAAGAGATGAG
CATGTCCTGCTTTCCGCCGGGCACGATGGCCAGGTATTTCTGTGGGACATCGAGCAGGGC
GTTTGTGTGGCCAACTTCCTTAACGACATCGATGGCCAAGGCCATGGCAGTGTCTTTGAT
GCAAAGTGGTCACCGGATGGCACAATGATAGCTGCCACCGATTCGCATGGACACATCCTG
ATTTTTGGTTTGGGCGTGTGCATTGAAAAGTACAAAATGCTTCCCACGGAGCTGTTCTTT
CACACGGATTTTCGACCTTTATTGAGAGATGCGCAGCACCATGTGGTGGACGAGCAGACG
CAGATAATGCCGCACCTAATGCCCCCGCCTTTCCTGGTCGATGCAGATGGCAACCCGCAT
CCATCAAGGTTTCAACGATTTGTGCCTGGTCGTGAGAGCTGTAGCCTAGACCAATTGATT
CCAAATCTTACGGTAGGCGTGGATGGCGTAGTAATCGACGATGGAGGTGCAGGTGGAGAT
GCTGTTGTGGCCGCTGCACCCGCTGAAGCTCCGCCAGCCGCATTGCCCCCTGCTGCTGCT
GGAGGACCACAGGGAGCTAACTACTCCCATATCGACCGCATGATTGCAGCCTTGGCCAAT
CGGCAGTCGGTTAATGTAAATGCCAATGATGCCAATCCGACCAACGGCAACCAGTCCTTC
GAACGTTTGACCAATCGTCAAATGCCGGACTCCAATGGCAGGCAATCGTCACGTGGCAAT
GTCCTGGGTAGTCGTTTGAGCACGCCTCGCCAAGGATGGGGTGCCGCAGCTGCCGCGCCA
GTTGAGGATCAAGCAGGGCCGTCTCAACCTCCGGCAGAACCTGCAGCCGAACCTGGCCAG
GCACAAGTCCAACAGGCGCTGCCTCTGAAATTCATCCGTCGCACTTATGTGCGTCCCATG
AAGTATCCCCAGTTGCAAAACCTTAAGCAGACTATTTACTCTGCCGGTCAGTTTGAAAAG
CAGGAGTACAAACGCGAAATGCGACGCCGACCCATCATGATAAACACTGCCAGTGCAGCA
TCTTCGCAGCAGCCAGGAAGCGTTGGCAGGCCACGAAACACACGTGGCAATGGCGGCGGC
GCAAGAGCTGGACGAAGACGTGGCCCCCAGCCAGCTGGCGGACAGCCACAACCCGCTTAT
CGCACCCGCGCTGTTCGGGATCAGGAGCCGGAGCACTACGATGAGGTAGCGCCGCCACCC
GAGGAAGAGGAGGAAGATGTGTCCAGCAACTCGTCCGGCGACACGAGCTACTCGAATGTG
GAAGAAAACCTAGAGGACAGCAGCGATGAGTCGGAAACGGACAGTTCCGATTACTCTGAC
TGGGTGGCGGATACTCCTGGCCCGAATCTGGAACCACCAAAACGATCCAAGCGTAAGCCA
CTCTCACGGCGACCCCGGAGTAGTGATGACAGCAGCGATGATGAGCGAGCGGGCAGATCC
AGAGGTGGGCAGGTTACGGCCCGGAAGGTTGGACGTAAAACTGTTTTGTTTCCGCCCACA
GATGCAAATGGAGAGATACCGGAGCTTTATCGTCCCGCTGAGTGGCTATCTGAGGTGATT
CCACGTAAGGCACCCTATTACCCCCAGATGGGTGATGAGGTAGTCTACTTCCGGCAAGGA
CACGCAAAGTACTTGGATGCAGTCCGCCTGAAAAAGGTCTACAAGCTCTCGCACAGCTCG
GAGCCGTGGAACTTTCACACACTGCGCGACCACGAGCTGGTGCGTGTGATTGGAATCAAG
TACGAAATCCGCCCACCCCGATTATGTTGCTTGAAGTTGGCCATCATCGATGAGGAGGGC
AACATGACGGGGACAACGTTCAAGATCAAGTACCATGACATGCCCGACGTTTTGGACTTT
TTAGTGCTGCGGCAGACCTTCGATCTTGCAGTGCAGCGCAACTGGAGCATTGGTGACCGG
TTTCGCTGCATGATTGTCGATGGCTGGTGGATGGGTCAGATTGAATCGCGCCACGCCCTC
TCTGCTGACTTCCCCGACTCGTCCTTCATGTGTTTCCGCGTGCGCTGGGATAATGGAGAG
TATGAGTTTATGAGTCCCTGGGACATGGAACCCATCGATCAGCAAAGAGTACCGGACGAG
GTGGGAGGTGCTGTTCCAGTTCTACCGGAGGAGATTCGCGCCACTCTATACCAGCCCAAG
TCCGAGGAATGGCACAGAGGGGATCGCGATGGCACCTGCAGGCGAATTATCAATGGATTG
GAGCAGGTTATGCGTCTCTCTATCGCAGAGCATTTCCTGGCCCCCGTGGACCTTAACATA
TATCCAGACTACGCTTATTTGATCGAATATCCCATTGACCTGACCACAATTAAATCTCGC
TTTGAGAATCACTTCTACCGCAGAATAACCTCAGCCCAGTTCGATGTACGCTACCTAGCC
ACCAATGCGGAGCAGTACAATCGCCGACACGCAATTATCGTCAAGCATTCGCGAATTGTG
ACAGATCTTTGTTTGAGGATCATCAGGGAACCGGATGAGATAGATGTGGCTGCTGTTTAC
CATCAGCTTGCGGATGTGTACCATTCGTCCGAGTCGGAGAACGAGGTAGATTCGGATGTA
GTGCCATCGACCAGCACAGGGCCAACTACTTCAGCAGCTGCAGCAGCAGCTAGGCAAAGA
GTATCCTCAGCCAGGCGATCCACTCGAATTCACTCGGAAGGAGACTGGCGTGCCGATTGT
CGTCAGCTGCTTGATCTTATGTGGGCGAGGACAGATTCGGCGCCGTTCCGCGAGCCCGTT
GACACCATCGACTTCCCTGACTACCTGGAGATCATAGCTACTCCAATGGATCTGCGCACT
GTAAAGGAGGATCTGCTGGGCGGCAACTACGATGATCCGTTGGACTTTGCCAAGGATGTG
CGGCTGATCTTCCAAAACTCCAAGAACTACAACACCAACAAGCGCTCACGGATTTATGCC
ATGACCTTGCGACTCAGTGCGCTATTTGAGTCGCATATCAAGACAGTGGTCACCAGCTGG
AAGGCGGCATTAAGGCGCGCTAATAAAAACAAGTCCGGCAGCAGTGGAGGAGGACGCGGT
GGTTCCAGCAGCAGTCCCAACAAGCGAGTCAAAAAGGAGCGATCCACGCGCCGTAATCAA
AAGCCAACAGCAGCGGCCAGAAGATTGCCAGTCGCCAACTCCAGTGAAGATGAGGACGAG
GAGGATGATGATGATGACGACGATGATGATGATGAGGAAGAGGCATCAACGAGGGGGCGC
TCTCGTCGGCGAAACGGTGTGGCCGTAAGTGCTAGTTCCTCTGGCTCCAATCGCGTGACT
TCTTCATCCGCCGCCCAACAGCGTCGAGGCAAAAGCAGCAGTAGCGAGGAGGAAGAGGAG
GAGGAAACCAGTGCGCAGGCCACGTCGGATGCCAGCAGCGATGAAGAGGAAAGAGCCCAA
TCCAATGACAGTTCGCCCCAGCAGACACGCACGCGCAGGGCAAGGCAGCGGCAACGGCTG
AGCGATGACTCCAATATCAACGATTCCGAGGATAGTTACAACCCCAACGAGGGACGCGGC
AGTCGGCGACGTCCAGGTGCCGCACGCCCGTCCAGCAATAGGCAGACAAATGGCCACTCC
AGCAAGAGGCGACGTTTGAACAAAAGTCCAGCCAATGCTGCACCTGGCCCTAGTTTGCCA
CGTCGGACACGTCGCATAGCAGGCAGCTCCGCAGAAGAGGCTGCATCTCACTCACAACAC
GATGAGAGCACACAGGATAGCCAGATAGGACGTCGAAAGGGACGCTCGGCAAGCAGCAGG
TCAATTGGCAATGGACCAAGTACCAGTGCCGCAGCTGCCGCCAACTCCAGCCTTGAAAGT
CCGTCACGGAATACTCGAGCCAACGGAACCAGGACCTCTTCGCATCTTGGAGCCGAAAAT
GATCACAGTTACCACCTGCCAGTGCGCAACGGGCGCATTATCGAGTCCGATACGGACTCT
CCTGTCATCAGACCCACGCGACAGAGCGCCAAGAGGGCGCTAATGGACTGGGGTCGTGGA
AGCGATATTGAAGAGGAGGAGGAGGAAGAGGAAGAAGACAGCGAGGAAGCCGAGGAGATA
CTGCCAACACCTACAAAGGACGACTTGCCCTCTACCAGCCGAGCCGCTTTGGCTCAGGCG
GCTGCTGGTCCTTCTCGCCAGCTACGCACGAACCGCAACACCGTCGTTCGTCCAGCCCAG
AGTGACGACGATGACGACGACGACAGTGACAACGAGCCGCTGGCCAACAATCAACAAAAA
AATTATCAATCACCTGCAAAATCTAGCTCAACCGCAACAGTGGCCCCTCATCCACTGAGC
CTGCGCCGTCGATTAGCCTCGCCGCCAGAACGGAATACCCATATGACCCGTTCCCATGCC
ACCTCCACAACGTCCTCGGCCGCAGCATCACGGGCAGAACAACAACCAGCGGTTTCCACC
CACGATCACAACTACCTAGGCAACGTGAATTCATCGAGGGCACCGCGCCGCATTCTTTCA
CGCCACCAACGAAATGCCGACGAGTTGGATACCAGTCTAGATGTCCTGGAAAACTCGCGT
CTGCTCTCAGCCATAGCCAATCACCGGCGACCCACCTCTACCACGAACAGCAACAGTGCG
AGCAACGTGGTTACAAGGCGTCTGCGTGGTCGCGCTAGCCAGGCGAATTCGCACGACGAG
GATCATGAGGAAGAGGAGGCTGCTGCTTCCGATGAGGGCACTGATGGAGGCTCTAGCTCG
CAGAGCTACGAAGATGAGGATGAAGACTCCGACTCGGAAGACAATCAGCCGCTGACCAGC
TACGTATCGCCCAGCAATGGTCGTCCGAGGCGAAAGGCAAAAACCTCCTCCTCCTCCGCA
ACAACTCAGGAGCGTCCGCTGCGACGCACACGTCGCCCGCCATCCAATAACTCCATCGCG
AACGACAACGATGACGATGATGACTATGTTGTGCCGCGGAGTCGTCGCGCCAACCGGGGT
GGAAACCAGCGAGCCGGGCGCAACCAAAAGAGACCGCGCTACAATGAGCAGTCCGAGGAG
GAAGAGGCGGCGGCTGGATATTCCCATCGGAGTAAACGAATCCGTAACGGCGACGCTCAT
GCCAGCTCTCAGGAGCGATCACAGGCCTCCACCGAGCGGGACCAGGAGGAAGAAGAGGAC
GGTACTTCGGTGGACTCGGAAGAACAGCAGGTGAACGTTAGCAGCAGAGGCAGGGTGCGC
AAGCCGTCTGCCAAGGCGCGCGGTATCTTCAAGGAG
Protein sequence predicted based on nucleotide sequence
METRQPSSGDDLKPELYFLISKFLAAGPLEETAKVLIRELEEKKVLPRRLDWQGNEHEQT FEELERKYKHIGANHLLEICSRLGPLVDRELPPSVPGINSLLGTGRQNLLRTKETVYCHR SLRDYCTRLNGVSLPDSVLTKPTHNLDRVLTGREHGGEVRRKLLVPTDLYRRTKLLRRTV GHLSSVYCVLFDRTGRYIITGADDLLIKIWSAADGRLLATLRGASSEITDIAINLDNTML AAGSLDHILRVWDMQTTSPIAVLSAHTGMITSVNFCPSPRSDLKYLVTTSTDGSIAFWQY STPRGQKITFAPKPTQYHEKLRPGQAQMMCTTFSPGGIFLAAGSADHHVRVYMMGEDGPK RILETEAYTDAVDSVQWSHRGLRFISGSKDGTAHIWTFESQQWKSSKLCMTERLASCPEP EEGKRLKVTMVAWDASDRYVITAVNDFTIKIWDSKSAKLHRVLRGHKDELYVLESNPRDE HVLLSAGHDGQVFLWDIEQGVCVANFLNDIDGQGHGSVFDAKWSPDGTMIAATDSHGHIL IFGLGVCIEKYKMLPTELFFHTDFRPLLRDAQHHVVDEQTQIMPHLMPPPFLVDADGNPH PSRFQRFVPGRESCSLDQLIPNLTVGVDGVVIDDGGAGGDAVVAAAPAEAPPAALPPAAA GGPQGANYSHIDRMIAALANRQSVNVNANDANPTNGNQSFERLTNRQMPDSNGRQSSRGN VLGSRLSTPRQGWGAAAAAPVEDQAGPSQPPAEPAAEPGQAQVQQALPLKFIRRTYVRPM KYPQLQNLKQTIYSAGQFEKQEYKREMRRRPIMINTASAASSQQPGSVGRPRNTRGNGGG ARAGRRRGPQPAGGQPQPAYRTRAVRDQEPEHYDEVAPPPEEEEEDVSSNSSGDTSYSNV EENLEDSSDESETDSSDYSDWVADTPGPNLEPPKRSKRKPLSRRPRSSDDSSDDERAGRS RGGQVTARKVGRKTVLFPPTDANGEIPELYRPAEWLSEVIPRKAPYYPQMGDEVVYFRQG HAKYLDAVRLKKVYKLSHSSEPWNFHTLRDHELVRVIGIKYEIRPPRLCCLKLAIIDEEG NMTGTTFKIKYHDMPDVLDFLVLRQTFDLAVQRNWSIGDRFRCMIVDGWWMGQIESRHAL SADFPDSSFMCFRVRWDNGEYEFMSPWDMEPIDQQRVPDEVGGAVPVLPEEIRATLYQPK SEEWHRGDRDGTCRRIINGLEQVMRLSIAEHFLAPVDLNIYPDYAYLIEYPIDLTTIKSR FENHFYRRITSAQFDVRYLATNAEQYNRRHAIIVKHSRIVTDLCLRIIREPDEIDVAAVY HQLADVYHSSESENEVDSDVVPSTSTGPTTSAAAAAARQRVSSARRSTRIHSEGDWRADC RQLLDLMWARTDSAPFREPVDTIDFPDYLEIIATPMDLRTVKEDLLGGNYDDPLDFAKDV RLIFQNSKNYNTNKRSRIYAMTLRLSALFESHIKTVVTSWKAALRRANKNKSGSSGGGRG GSSSSPNKRVKKERSTRRNQKPTAAARRLPVANSSEDEDEEDDDDDDDDDDEEEASTRGR SRRRNGVAVSASSSGSNRVTSSSAAQQRRGKSSSSEEEEEEETSAQATSDASSDEEERAQ SNDSSPQQTRTRRARQRQRLSDDSNINDSEDSYNPNEGRGSRRRPGAARPSSNRQTNGHS SKRRRLNKSPANAAPGPSLPRRTRRIAGSSAEEAASHSQHDESTQDSQIGRRKGRSASSR SIGNGPSTSAAAAANSSLESPSRNTRANGTRTSSHLGAENDHSYHLPVRNGRIIESDTDS PVIRPTRQSAKRALMDWGRGSDIEEEEEEEEEDSEEAEEILPTPTKDDLPSTSRAALAQA AAGPSRQLRTNRNTVVRPAQSDDDDDDDSDNEPLANNQQKNYQSPAKSSSTATVAPHPLS LRRRLASPPERNTHMTRSHATSTTSSAAASRAEQQPAVSTHDHNYLGNVNSSRAPRRILS RHQRNADELDTSLDVLENSRLLSAIANHRRPTSTTNSNSASNVVTRRLRGRASQANSHDE DHEEEEAAASDEGTDGGSSSQSYEDEDEDSDSEDNQPLTSYVSPSNGRPRRKAKTSSSSA TTQERPLRRTRRPPSNNSIANDNDDDDDYVVPRSRRANRGGNQRAGRNQKRPRYNEQSEE EEAAAGYSHRSKRIRNGDAHASSQERSQASTERDQEEEEDGTSVDSEEQQVNVSSRGRVR KPSAKARGIFKE
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
