Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
4255
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00770524
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2991nts
Open reading frame: 1 to 2991
ATGAGATTCCAGTGGTTCTTATCGGCCAGTGCGAGCTTGTCGCTGTTCCTTCTCCTGGAC
TTCGTCGTATGGATTCACGGCGAGCGGGACTTCAATGTCAACGACTCCCAGGTCCCTGTA
ATTGAGCCAAAGGATGTGCCCGCCTACAAACAGGATCCGTATGTTACGGAATTGATGAGT
TGCCAAAATACTCCGAGTGAAATTGTGCTTTCACTTTTGTTGAAGAAGCACGACTGGAGT
GAGCTGACGGCCACGAAACGAGCTCATGTCCAGGCCAAGTTGGCCAAGTTCTTTGCCATA
CCCAAGGAATTCATTTCCCTGGATTCGGTGTCTAAACGCGAACTGAGATCCATGCACAAG
TTGGCCATGAGAAAAGGAGGCAAGGGCAACAAGAATATCGAGACCCTGAATCGTCGCCTG
GGACGCGCCAGTTTCATGATCGGCTGTGGTCCAAGCTACTTTGTGATGGGCGAACCGATA
GCCAAGCAGATTGCCCACCAAATGAAGGATGGCACCATTGGCGCTTTGACGGAGGAGAAC
TTCGGGCTGTGGTTCATTTGGCGAAAGGAATTGAAATCAAGATCAAACCGCAAGCGACGT
CAATCCGAGGGCTCTGGTGCCGATGAAGATGACTACGACTATGGGGATGACGATGAGGAA
GTTGCTGAGCCCAGTACGGAAGTGCCACCAGTGACCACACATGCCCATCGACATCATCAC
GGAGCGCCAAATACTGTGCTTAATGAGCTCGAGCTGAGCACCCAGCTGCCCATCGACGAT
TTTGAAGGCACTGCTACTGCAACACAGCCAACTGGCACTGCAACTGCAACTGCCATTGCA
AGCACAGCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAACCGCAACAGCCACAACTGCC
AATGCATCTGCAACAACAGCAGGCGAAAGCGAATTTCATATAGAGTCCACAACGCACCGC
ACGATTAGCTCTAAGGAAGGAGGAGAACCCGATGCCATCAGCAGCGGCAACAATAGCCTG
GCCAATAATGAGCTGTCGACGCCCGCCACGCCCACATCTTCGGTGGCCACGACCACGGCA
TCTACTCCGGAGTCGAGCAGCATCAGCAGCAACTTTGTCTCCACCGACTACATGGAACCG
CAGCCGGAAGAGAATAGTCCGCCCATCATCAAGACACGCCTGCAGAAGCTGGCTGTCACA
TCGGGCAAGGCCTTCACCTTCCATGTGCTACCGGAAACGTTTTACGATGCCGAGGATCAG
GGTAATCTCCGCCTGGCGCTCACCGACAAGGATGGCCACGAGCTAAAGGCGAACTCTTGG
CTGCAGTTTAACGCCGACAAGAGGGAGCTTTATGGCCTACCTCTGGACGACACGGTATCC
CGCTGGCAATATCGCCTGTCGGCCACGGATTCGGGCAACGCCAGTGTCACGGAAACCGTG
GAGATAAGTGTGCAGCAGCATCGAGCGGTTAGGACCATTAACCACGAGATCAGTGTTTTT
GTACGGATCAACGAGAAGCCGGGTCACAACATTGATTGGCAGTTGAAGCTCATTAATGCG
GTGGCCAGAACCTTGGATGACTCCACCAATTCGGCAGTGGTGGTGCGTGAGATCCGACAG
ACACCACACGATCCACACAGTGCCACCTTCGTGTATTTTAATGAAACGCTGCCCACCAGC
GAGTGTCCCGAAAAGGAACTAAAGGATATTATTGCACGACTGGATGCCAATAGACTGAGT
GATTTGGTTCAGCCACAGCTGGGAATCAAATCTATAACAGGACAGTTAATCGGATCCTGT
CAGAAGGATTTAACTCAGGTGAAGCCCACGCAACACATGACCAAGAATGTGCCTCCAATG
CCACGCAACCAGGTGGATCGCGTTAACGCTAGCTTGGGCCAGCTGCTGGTCTACAAAGTG
CCGGCGGATACCTTCTATGATGCGAACGACAATCAATTGACTCTTACGTTGAAAACCAGG
GATCACCTTGAGCTTAGCCCACGCCACTGGCTGCAGTTCGACTCCAAGAACGAAGAGTTC
TATGGCATTCCGAAAAGCGGTGACATTGGTTCCGAGGAATATCTGTTGGTCGCCGAAGAC
AGTGGTGGCCTGAGTGCCCATGATGCCCTGGTTGTAGTGGTTAGCCCAGCGCCAAAGCGT
GACTTTGGGTTCTTCTTCAAAGCGTATCTGTCTATCAAACACGAGCGATTCAATGCCGAT
CTGCAGCGGAAGTTTGTAGAGCGTGTAGCGAAGCTGAATGGAGATCCTACTACTGGCCAG
ATCCAGATCCGTTCGATAACCACGCACCACGACTCCGATGGCACCATAGTGAACTTCTAC
AATACGACGCTGTACAGGAAGCACAACAGTTGTCGGGAGAAGGAAGTGGCCATGACTAGG
AGTGTCTACCTAAACAGTGACCTCAGCCTGAGGGAGGCAGCCAAACGGGCTCTGGGACCC
GAGCTCAATTTGACCAACTTTTCGGTGGTGCCCTTCAGTATTTGTCATCATACTGAGAAC
ATAGACACCAACCAACTTGATTATATACCTAGTCGCCCCGAAGAGCCTACACATAAGTCC
TCTTTCGGCGAGGATTACATGATCACGTTCGTATGGCCCATCGTGATCATTGTGGCGATG
CTTGTGGCTGCCTCAATCATTGCCTGTTGCCTGCACTGGTGTCGCCAAAGGAGCGGCAAA
ATGGAGCTAGGCGACGAAGAGGAGCGCAAGTCCTTCCGTGCCAAGGGTATTCCCGTCATC
TTTCAGGACGAGTACGAGGAGAAGCCCGAGATCGGCAACAAGAGTCCTGTCATACTCAAG
GACGAGAAGCCGCCGCTGCTGCCACCATCCTACAATACCTCAAACATGAATGGTGACAAC
GACGTGGACGATTATGTGCCACCACCATCGGTGGTTGTGGGCGGCCGGGAAGTGCGTGGC
AAGTCGCCAGCCACGCCCTCCTACCGCAAACCACCGCCATATGTGTCGCCA
Protein sequence predicted based on nucleotide sequence
MRFQWFLSASASLSLFLLLDFVVWIHGERDFNVNDSQVPVIEPKDVPAYKQDPYVTELMS CQNTPSEIVLSLLLKKHDWSELTATKRAHVQAKLAKFFAIPKEFISLDSVSKRELRSMHK LAMRKGGKGNKNIETLNRRLGRASFMIGCGPSYFVMGEPIAKQIAHQMKDGTIGALTEEN FGLWFIWRKELKSRSNRKRRQSEGSGADEDDYDYGDDDEEVAEPSTEVPPVTTHAHRHHH GAPNTVLNELELSTQLPIDDFEGTATATQPTGTATATAIASTAAAAAATAAAATATATTA NASATTAGESEFHIESTTHRTISSKEGGEPDAISSGNNSLANNELSTPATPTSSVATTTA STPESSSISSNFVSTDYMEPQPEENSPPIIKTRLQKLAVTSGKAFTFHVLPETFYDAEDQ GNLRLALTDKDGHELKANSWLQFNADKRELYGLPLDDTVSRWQYRLSATDSGNASVTETV EISVQQHRAVRTINHEISVFVRINEKPGHNIDWQLKLINAVARTLDDSTNSAVVVREIRQ TPHDPHSATFVYFNETLPTSECPEKELKDIIARLDANRLSDLVQPQLGIKSITGQLIGSC QKDLTQVKPTQHMTKNVPPMPRNQVDRVNASLGQLLVYKVPADTFYDANDNQLTLTLKTR DHLELSPRHWLQFDSKNEEFYGIPKSGDIGSEEYLLVAEDSGGLSAHDALVVVVSPAPKR DFGFFFKAYLSIKHERFNADLQRKFVERVAKLNGDPTTGQIQIRSITTHHDSDGTIVNFY NTTLYRKHNSCREKEVAMTRSVYLNSDLSLREAAKRALGPELNLTNFSVVPFSICHHTEN IDTNQLDYIPSRPEEPTHKSSFGEDYMITFVWPIVIIVAMLVAASIIACCLHWCRQRSGK MELGDEEERKSFRAKGIPVIFQDEYEEKPEIGNKSPVILKDEKPPLLPPSYNTSNMNGDN DVDDYVPPPSVVVGGREVRGKSPATPSYRKPPPYVSP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
