Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
6730
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00771484
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5859nts
Open reading frame: 1 to 5859
ATGGGCGACAGCGAACAGATTTGGCCAGAAATCGGAGTGGATCCGCAACATGTTTGTGAG
GCAGGCGGCGATGGTAGATTCCAAGTGATCTGCGACAAGGCGTGGCTGCAATCGCTGGAG
CGCAATAGGCGGCTAACACACTTTCCCAGTTGGCCACTGCTGGATCGCAGGTCCTATCCC
ATTGGGCAGTTGGAGCATCCGTGGACGAGGATTTTGGTCCAGACGTATAGGAAGCAGCCG
CAGTCAAGGGTGTACCCGCTGCCTAGGATCTCTGGGGATGCGCCTTCATCCGAATTGCCA
CTCTCCTACTCGCAGGCAGTATCCTTTGTGGCTCACAGCAACTTCAAGCAGCTATGGGAG
GAGGCTTACAGGAAGTACAATGGCGCCCATGTCCGAATGTGCAGATCAACCCCGTCAGCG
CCCTCGACGAAGCCACCAAGTGGCCAAGGCCAGCTACAACCGCATGATGTGGTGCTCAAA
GAGCTCATCCAGCGGGTGTATCGTTGCCCCGTGCTGCACTGTCAGCTAGATCGTGACGGA
GTTAGTGACTCATCGAATGCTACATCTTGTTCCGCAGGCAGCTGTCATGCCAACGTCTTG
CCCGCCCTGGTGGCCATAGAGACCTCCACGCACATCAGCGTCTTCTTTTATCCTCCAGCT
CTGGAATGCAGCCTCTACGATTGCATCACCTTCAGTCCGGCATTGTTGGGGAAGAATTAC
AACAAGACACTGTTTGTGATCTACCAGATTCTGCAGCTGGCAAAGCATCTCCAAGGACAG
GGACTTTTTCTGGGTGATCTGCGTCTGCAGGATATTGTGCTGCGCGAAAATCTCTGGCTG
CAAGTGCTTCCACGGCTGCAGTGTAATCTGCTGGACGATGATCCGGCTGGGGAGGACATG
TCGCCCCTTACGCCGCTGGTCAGCGAGGATTTGGGAGAGGCTAGGGAGGCGCAGCAAGCA
CTACCCTCCAGCAGCACCATGTTCGACCTGCGATTCGCCTACGATCCCGCCCACTTCAAC
TTGCGCGAATATACCGAGATGTGGTGCAATGGTCAGTTGTCCAACTTCGACTACCTTACT
ATCCTGAACAATGCGTGCGGCAGGAGTCTGACCAATGCCGCCTACCACCACATCATGCCC
TGGGTTACTGACTTTAGTGGACGCGGAGGAGGCGCATGGCGGGATTTGACAAAGAGCAAG
TATCGCCTGAACAAGGGCGATGTCCATCTGGATCTCATGTACGCCCATGCAAGCCAGCAT
GGTAGCGCAGACGGTAGTTATCAAGCAATCGGTGATCAGGCGCCTCATCACGTCTCCGAC
TTTCTTTCCGAGATCACCTACTTTGTATATATGGCGAGGAGAACGCCCCAGTCCATTCTC
TGCGCCCATGTGCGTCCCATCTGGGTGCCCGCCGAGTATCCTGTGTCCATTCAGCGACTC
CAGGAATGGACGCCAGACGAGTGCATACCCGAGTTCTACTCAGATCCCATGATATTCAAG
AGCATTCACGAAGACCTGCCGGATCTCGAGCTGCCTGCTTGGGCCACCTGTCCGGAGGAT
TTCATCTGCAAGCACCGCGAGGCCTTGGAGTCGCAGTACGTGAGCGAACGACTGCATCAC
TGGATCGATCTCAACTTTGGATACAAACTGAGTGGCAAAGCGGCAGTTAAATCGAAGAAT
GTCTGCCTTACTCTTGTGGATCAGCACCGGAATCTCAGCCAGCGCGGCATCGTCCAGCTC
TTTGCAAGCGCACATCCTCCACGTCGATATGCGACGCCCTGGTTTAGTAGAACCGCACCC
AGGCTAAGCCAGCTGTACGCCAGTCCTGCAAAACGTCTAGCCAAAAGTACGGAAAACCTA
CAGGCTGAGAGTGCAACTGGATCATCTCACCTATCTTTGCGTCTTGGTGATGACGGATCA
AGCGGCTCTGTTCACAGCAGTTCTGCATCCGCATCAGCTTCCAATTTCTATGCAATCACA
AATTTCATTGAACTGCCGGAGAATTATAATCCAGCTCTTTTGCTACACAATCTGGAAACT
CTGGAGGCGTTTTACGCACGCACATTCCCACAACAACGCGCGTCTGCCAATCCCGAGGAG
AAGATGATCAGCAGTGACTTGATGTTCGAGCAGAACTCAGCGGAACATTCGTTCACGAAT
CAACTGTTTGCCAGTGGTGATGAGCTACCACCGAAGTCAAAGAATCTCTTAAGAGAAAAA
AGGAATTGTCTACAGCATCTGCTGAACGTCAGGAAAGAGCGGGATCTCCACGTCTTGGGA
TGTCTCATTATTGAACTCTTCGCCATGCAACGTTTGCGACCTCTTCTGATGGGAAATGGC
TTGACTGCAACCTTTGATTCCCGACTCTCCGCCTGCCGGACGGTGGCTCAGCTGCATCGT
CAGGAGCTGCCAAAGCCAGTGCGCCGAGTAGTGCGCCTGCTGCTTCAATTGGAGCAGCCT
GGAGAGGAGGATCAAGGCTTGCCACAGCCGCCAAATCCTGCCCAGCTGTTGGAGCCCATG
TTTAGCAATTTGTTGCTTCCATTCCCCAGCCACTACATGGCTGTCTACGCTCTTGTCAGA
TCTCTACATGCTTTTGAGGCTAATGCAGTGTTACTGGAGCTGCAAACTCACTTCAGCTGC
AAAGGAGGCAGGGAATGTGAAAGATACACGGAGATGGATCGGCAGAGAGTTCTTTTCGAA
CGAAAGATCGCCGAGTGCAAGGTGATGTCGTGTTGTGCCCATGTAAAGAGGCTTCTAGAG
CCCATGTCCTTTGCCTATGAGCAATTTACTCCGGTTGAGTTGCTGCTACCGCACATCATA
GATCTTTTGAGAGATGAACGCACATCGATATTGACTGCCTGGAACCTGTTCGATCCCATT
GCACAGGCATTGGGCGTATCCCAAACCCAGCAGCATTTGCTACAACCACTGCTCAAGCTG
TACGATGTGGAGAGTGTCAGTTCCCCGGACGACGGTGGCTCCAATGGCTCCACTAACAGT
GGTGGCCATCTTCGTTTCTCGGCCAGCAGTTCGTTCAAGTCCCGGAAATCGGTGAAGCTT
TATCACCACAGTTTCCTGCTTCGTCTAATTGTGCGTTTTGGACTCCGCTGTTTTCTGCAT
CATTTCATAGCGCCACTGATTGAAGCTGTTGGTGGCTACAAAGAACCGGAACAGGGCAAT
GGTTATCACTATCACAGTGGCGGAAGCAGAAGGACGAGTAAAAATCTTAACTTTGCCTCA
GAGAATGAAGTTCATCAGACAGAAGTGGCACCAGAAATGAAACCCGACATTGAGGAGCTG
TTCACCTTCGAGGATGACCACGACAGGGCGTCAACGCAGGACAGCAAGTCGATTGACTCC
TTTGACATGCGTCCCAGCACCAGTGCGGCTAGTGCGGAGGAAGCTCGAGAATCGGATGGA
TCCCCAGATAAACTGGTGATCAACGAACTAATCTATGGAGCAAGAATATCGCCAGATAAA
CTATCTTTAAAGGAGAGTCAAACACCACCCGCATTGGTACCACCTCCCATAGGACCGCGA
TCGCCCACCATTGAGATTCCCATGGGTCCCACTGCCGGTGGCATTCGTCGTAGTTTTCAG
CTAAGCGCCATTGACTGCGATATTGGTTCGCGGAAGAGTGTGGACAGCTTTGATCTGATC
AGCCAGGCGGTGGAGCAGCTACCTCCTCCAGCGGAGGAGTCAGAAGCGGAGGCATCGGAC
TCCCTGCAGGCATCGGTCATCTCTCGGTTGTCGGAGGCCAAGGCCGTGCAGAACAATCGC
ATCAGTGAGATGAGTGCCGAGAGTTTGGTGTGGCTGTCGCATCGGTTGGGTCCGGTGCTC
ACTTCCCGATTCATCACCAGGAACCTGCTCAAGCTGCTATCCCTGTGCTATGTGGGTCAG
GAGAACCTGCTACCGGAGGTGGACGATGGTGGTTCCAGTTTCAATTCCAGTCCCGATGCA
GCCAATCTCAATTACTTCAGCATTGCCAACGCGAGGGTCGTGGGCGACCGGAGTGCAGCT
CGCGTCCTGGAGTGCCTCATGTCCATTGCGGCTCTCTATGGGGAAAACTTTTTGCTGTTG
CAATACTTTCCCCACATCAGCGAACTCATCAGTCTATGCTCCAAACGAATAACTGGCAGC
CTCGAGGGAGCCATAATCAGTTCGCTACAATTGCTTAAATACATGGTTCCGTGTCTAACC
GACGCCAGTATCATGGAGCACCTACAGCACATAATGCTAGACGCAATACTTCTGCCCATT
TTGCGCCTTTTGAGCTCCACCAACCTTGTGATGCCCAGTGGTTATCTGGGACGTTCGCTG
TTGGCACGCAAGTTTCTGGATGCGATCTACGCTCTGAGCGTTCGCTTGGGTTCGGATATG
ACGCGGGAGCACCTGTGTCAGCCGCTTCTCAATCCATTTTTCCTGATTTTCAACAAGGCC
TTTGGATTGCCAAATGACTTTGCCTGCGATCTCGCTAATCTCTCGCTCGTTGGTGGTGCA
TCGCAGCCGGAACGAGCTCTGGAGGAGCTAAGGGATGTTTTCGGACCGGAGCTGGCGCAC
ACCGCTTATCTTACATTCCTGCGCTTCTTGGGCGAGGCCAACATGAAGAGGACACTGAGC
AATCTGGATTTTGTGCTAACCTTATGCCACGAACACGAGCAACCCAATGGACAAAGCAAA
ATTCAACTAGCGACCAATGTGAGTTCTGGGCAAGATGTGGACTCTGTAGATGCAAGCTGC
GAACTGGCCGCCAATAGCTTTGGCACTCAGATCGTGGGAAATCGCCTGCAAGTGGCCAGA
AACAATGTGGAACTCATCGACATGGTGGCGTATAAACTGGACCAGATGCCCAAAACTCGT
CATCTGAAAGGCAACTGGTTGGCCTACTGGCGCAATGAGACGACGCGCAATGAGAAGGAC
ACTCAGACACTTAATCTCAAGCAAATTCGCCTGCAGTCGTTTGTAGGACACACGAACTCA
GTGAGAGCTATATATGCCCTGGATAATGAGAACAGTTTCATTTCCGCCTCCAAGGACAAG
ACCGTGAAGCTGTGGTCACTTCGCAGCGAAGGAGATGGCCGCAAGACGAGCGCCTGCCAA
TTTACTTACACGGCGCACAAGAAGTCCATAAACTCGCTTGGCTTCCTGGAATCCTTGCGA
TATGTGGTGTCCTGCGATTCGGGCATTCATCTGTGGGATCCGTTTATAGGAAGACCGCTT
TCCGTACTGGATGCTCCTCGTCATAGTGCTGTCACAGTGGTCAAATGTTTGCCCTCCCAT
TCACCGCTGGTAATTGCCGGCACAGCGGAGTCCACTGTCAAGATGGTGGATGCCCGGAGC
TGTGAGTACGTGAACGAGTGGCGGGTGTGCAATGCTTCTCTGCCCAATGCAACTGTCAGA
TGCCTGGCTGTGGCCCCTTCCGGCAACTGGCTGGCAGCGGGACTCTCGTCAGGATGCATC
GTCCAGCTGGATACGCGCACTGGCATGGTGATCAACTCGTGGCGACCAATGGAATGTGAT
CTGCTGCAGCTGGCCGCTCCGAGTGATCAGTTCTTGGTTAGCTCCGCCTTAGATCATAGC
CTGGCCGTTTGGCATGCCCTGGATGGTATCATGCACTACCAGCTCAAACCTCCACCGGAA
CCCGCCCATTTCCTGCAGAGCGTTGGCCCATCTCTGGTCTATGCAACCACTGGCAACCGC
GTGGGCGTCTATGCGGATGTGGCCCACTCCCATGCCTTCAGCACCATCACCAAACTTCGC
TCGGAGACATTCCGCGGTGTGCTAACCTCACTGGCCGTGTTGCCACTGAATCGAGCCTTC
CTCGCGGGAAACGAGAGCGGAAACATAGCCCTGCTTTGC
Protein sequence predicted based on nucleotide sequence
MGDSEQIWPEIGVDPQHVCEAGGDGRFQVICDKAWLQSLERNRRLTHFPSWPLLDRRSYP IGQLEHPWTRILVQTYRKQPQSRVYPLPRISGDAPSSELPLSYSQAVSFVAHSNFKQLWE EAYRKYNGAHVRMCRSTPSAPSTKPPSGQGQLQPHDVVLKELIQRVYRCPVLHCQLDRDG VSDSSNATSCSAGSCHANVLPALVAIETSTHISVFFYPPALECSLYDCITFSPALLGKNY NKTLFVIYQILQLAKHLQGQGLFLGDLRLQDIVLRENLWLQVLPRLQCNLLDDDPAGEDM SPLTPLVSEDLGEAREAQQALPSSSTMFDLRFAYDPAHFNLREYTEMWCNGQLSNFDYLT ILNNACGRSLTNAAYHHIMPWVTDFSGRGGGAWRDLTKSKYRLNKGDVHLDLMYAHASQH GSADGSYQAIGDQAPHHVSDFLSEITYFVYMARRTPQSILCAHVRPIWVPAEYPVSIQRL QEWTPDECIPEFYSDPMIFKSIHEDLPDLELPAWATCPEDFICKHREALESQYVSERLHH WIDLNFGYKLSGKAAVKSKNVCLTLVDQHRNLSQRGIVQLFASAHPPRRYATPWFSRTAP RLSQLYASPAKRLAKSTENLQAESATGSSHLSLRLGDDGSSGSVHSSSASASASNFYAIT NFIELPENYNPALLLHNLETLEAFYARTFPQQRASANPEEKMISSDLMFEQNSAEHSFTN QLFASGDELPPKSKNLLREKRNCLQHLLNVRKERDLHVLGCLIIELFAMQRLRPLLMGNG LTATFDSRLSACRTVAQLHRQELPKPVRRVVRLLLQLEQPGEEDQGLPQPPNPAQLLEPM FSNLLLPFPSHYMAVYALVRSLHAFEANAVLLELQTHFSCKGGRECERYTEMDRQRVLFE RKIAECKVMSCCAHVKRLLEPMSFAYEQFTPVELLLPHIIDLLRDERTSILTAWNLFDPI AQALGVSQTQQHLLQPLLKLYDVESVSSPDDGGSNGSTNSGGHLRFSASSSFKSRKSVKL YHHSFLLRLIVRFGLRCFLHHFIAPLIEAVGGYKEPEQGNGYHYHSGGSRRTSKNLNFAS ENEVHQTEVAPEMKPDIEELFTFEDDHDRASTQDSKSIDSFDMRPSTSAASAEEARESDG SPDKLVINELIYGARISPDKLSLKESQTPPALVPPPIGPRSPTIEIPMGPTAGGIRRSFQ LSAIDCDIGSRKSVDSFDLISQAVEQLPPPAEESEAEASDSLQASVISRLSEAKAVQNNR ISEMSAESLVWLSHRLGPVLTSRFITRNLLKLLSLCYVGQENLLPEVDDGGSSFNSSPDA ANLNYFSIANARVVGDRSAARVLECLMSIAALYGENFLLLQYFPHISELISLCSKRITGS LEGAIISSLQLLKYMVPCLTDASIMEHLQHIMLDAILLPILRLLSSTNLVMPSGYLGRSL LARKFLDAIYALSVRLGSDMTREHLCQPLLNPFFLIFNKAFGLPNDFACDLANLSLVGGA SQPERALEELRDVFGPELAHTAYLTFLRFLGEANMKRTLSNLDFVLTLCHEHEQPNGQSK IQLATNVSSGQDVDSVDASCELAANSFGTQIVGNRLQVARNNVELIDMVAYKLDQMPKTR HLKGNWLAYWRNETTRNEKDTQTLNLKQIRLQSFVGHTNSVRAIYALDNENSFISASKDK TVKLWSLRSEGDGRKTSACQFTYTAHKKSINSLGFLESLRYVVSCDSGIHLWDPFIGRPL SVLDAPRHSAVTVVKCLPSHSPLVIAGTAESTVKMVDARSCEYVNEWRVCNASLPNATVR CLAVAPSGNWLAAGLSSGCIVQLDTRTGMVINSWRPMECDLLQLAAPSDQFLVSSALDHS LAVWHALDGIMHYQLKPPPEPAHFLQSVGPSLVYATTGNRVGVYADVAHSHAFSTITKLR SETFRGVLTSLAVLPLNRAFLAGNESGNIALLC
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
