Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
6610
Keyword:
None
Species:
Drosophila melanogaster
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The FlyBi Consortium: BDGP/Celniker, CCSB/Vidal, DRSC/Perrimon
DmCD00772300
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4992nts
Open reading frame: 1 to 4992
ATGGAAAGCACGGACAGCAGCGACAGCAGTGGGGATAGCTTAAAATCCATTCCCCGGCCG
GACTTTGAGGCCCTCTCCGCCATCGGCAGCCCACAGCCGCAGTTGCAGAGCTCCTCGCTG
GATGGCCACGTCGAGATGGAGCCGGTCATCATGCCCAGCAAGCGTCAAAAGTATATTGTC
AGTGCTCCGCCTGGGTCGGCCAACTGCAGCAGTAACTCATCCAGTTCGAGTCCCTCGAAC
ATCAACGTGGGCTCCTCGCTTACCATGAAGTTGACGAAGGTGCAGCCACAACAGCATCAG
GCTAAGTCCTCCACGCCAAACAACAAAGGCAAAGTAGCCAAGGAAATGGTTGCTCTGCAG
CGCTCACACATCGAATCCGAGGTCCTCAGCAACTTTGTGACTGGGGTGTCCAAGCTGAAG
AGTCGAAAGTCGCGATATGTGCCCGGGAATCTCGCCACTCAGGCAGTAGGTGGAGAGCTG
TCCCGCAGCTTAAATACCTCCTCCGAAAACGAAAACAAATCTCTAAAGGTTGCCCTTAAG
CGGAGCAGCAGCATTCCAGCCCCCATCCTCGACTCAGATGACGACTGGCAGGACGAGGAC
ATCGCCTGGGGCGTAACGACCTGCAGGCCGAGAGGACGATCGATGGCCTTTGAAGATGGC
GAGCGGCTGGCAAATCTAAATGCGGAGGACAGAAACATCGTTCCCGCATACTCAATGGCC
ACCCGCTGTCGGTCACGAAGCAAAACTCTATCCTTGAGCAACAGTTGGAGCAATGATGAT
AAGGGTCCCCGACGCAGCAACCTACGCAGCGAGAACGAGGAATTCGCGAAGAAGCATAAT
GCTTTTCTTGATCGCATAATCCACGATGACCGGGAATCGGAACTGGCGTTGAACACATCC
GGCGAAGGCGAAAGCTCGCTCTTCTCGGATGCCAGTGACACAAAGACGACCACGGCCAAA
ACCCCAGAGGAGGAGGCGGCCGAGCGGCTGGCTCTCATATATGAGGAGCCACCTACGCCC
GGCTGGGACCCGTTCTGCTGGAAGTGCCGAGGGTGCGGCAAGCTAATGCCGTGCTCCAAG
TGCCTGCGATCCTTTCACTCTTACTGCGTCCGACCTGCCACCACCAAGTTCGACTCCTCC
TGGAAGTGCCCCGAGTGTCAGGTTATAGAGGCGGCCCCCAAGAGACTGCGGCGCAATGGA
GTTTCGGTGGATCTACTAAGCCAGCTGCTTTCCTTCGCCCTGGACCGTATGAAGCATGTG
CGCGGGGCACATAAGCTACGGTCGCCGCTAGAGGTTTTTCCGTTGACGTACAAAAAGTTT
TTTGTGAACCCGGTGAGCTTCGAGAGCCTGGCGCAGTGCATCCGCAATGGGGCCTACCAA
AGCACAGACGAGTTTCTGAGCGAAGTGAAATGGATACAGCATAACGCTCTCATCCTGGAT
GCCGGCGATGCCAAAGTGGAACAGGCCTCAAAGGCTGTGGTGAAGGTATGTCGACAGGAG
GCCAACGAAATCGACACCTGTCCCGAATGCTATCTAAATGCCAATTCCAGCGACGAGTGG
TTCGTGAAGGTGTGCCGGCATCCGCATCTCCTTCTCTGGGCGAAACTAAAAGGATTTCCC
TATTGGCCCGCGAAAGCCATGGGTTCATCAAACTCAACTCTGGTGAATGTGCGATTCTTC
GGCAAACACGACCGAGCTTTCGTGCCCGTAAAGGACTGCTTTCTTTATTCGGCTCAAAAT
CCAAACACGCAAACCAGCCGGAGATCGGCAAGGGATTTGGCGGAATGCATAAGGGAGGTT
GAGATTCACATCGACCACATCAAGCGCAAGATTGGCGCTTTCAATTATGCTCCCTATCGC
ACCCCTTACGATCCTCTGGAAGAGCAACAACAACTGGAGCAGATGATGCCAGGCGTGTAT
GCGGCTATCGACCGGGAACTGGAGCCGGCTAATAAGACACCGCTGCAGTTTCTTATACGC
AAGACCGCGGACGATAAGCTCTCCATCGTCAAGAAAACAAAGGCGACAGAATCGGGAAAC
GAATCGGATCAATCGCCAAGTCCTACAAAAAAACTTTCGGAGGTTGATGTTGTTTCTGTA
ACAGGGTCCGGATGCAGCGATCACTCAAATGTGAAGAGCAACAACTATGAGGTTATATCC
AGATCTGGGGAATCCTTGACCGACTCTCGTTGCAAGGTGTTGCTCAAAAGGAAATCATTA
GCTGCCAAAATAGTTGCAGAATCCGTTGAAACTTCAGAAGCGGTGGCTCCGAAACGCAAG
CACTCTCTAAGTGATGCCAGTTTCACCAGCGAATCCAGCGAACACAAACGAAAGTCAAAG
CATGCTAGGAAACAACACGATAATCAGGATAATCAAATTGAAGAGGCAGAAAAAACTGGC
CAAGAGCCCCCAAAATCTCCTACAATCTCAAAACAGAATGAAAATTTACGAGATGAAGAG
AATGTCATAGAAAATGCTGCCAATGATACTTCCTCGGCATCCCCGGTATCTGCGTCTGTG
GTTTCTGTGGTGGAATTGGTCAGACGACGCCAGGGAGTGACCATCACAAAGATACCACGC
GAGCAACAGCAGACAGCAGAGGACACAGCTGCTGTTCCCATTCCTCTTCCAACAGCTCCA
CCTCCCAAACAGAATATCCCAAAAGGGAATGAAGCAGCAAACCCAAAACAATCTGATGTC
GAGAGACAACAAGAACAGCTCATCAAAAAAGTTATTCCGTTTATAGAAATTAAAACAGAG
GTGATGTCGGAGCCAGATGACGAAGGAATAGAGGAAGCGTCACCGGCTAATCAACCTCAA
ACTGATCAAGTTCCCTTGCAACAGGAGACAATTACAGCACAGCCGGAATCTCAAATGCCA
GCTGCCGCGCCGGCTCCCCAACCCAAGCCAGTGGATAATGCACCTTTTGAAGAAGTCAGA
ATCAAGGAAGAGATTCTCAGCGAAGATGAAATGGAAACGGAACAGTCTATTGTGAGTCGG
AGGTTGAAATCTGTGCCACCTATGCCATTGCCTATGCCCCCGCCTCCTCCTTTACCGCCA
AGAGAGGAATCACCCGCGACAGCTGATTCGGTTCGATTTGTGGGCGACACCACGATCCAA
AGGGTAAGCCAGAAACAGGGAGGCAAGTCCACGGATACGGGCGGAAAACGTAAAGGTGTT
CAACAAGTTCCCATAGCAGGTATACCCCAGGCACCCTCTCCAACTCACTCGCCCCTTCTG
TCAACCGCACCTTCACCTTCGGCATCACCAAAGCCAACTAGCACGCTTGCTGTTAGCAAG
CCACCGCCTCCGCCACCGCCCAAGGGAAAGTCTGCGTCACACCTTCAATTTCGAGGAAAA
AACGATCGTATCCCAACCTCCCCACCGGCGACTGCTCCACCAGCCACAAACACGTCTTCT
TTGCTCCGATCCAACATGGTAGTGATTCCAGTGGAGCAAGGCGGCAGCTGCAATGCACAC
AGCCCGATGACTATCCCGGTTCCACCATTAAGGGCCGTTTCAAAGAACACACTGCAGAAT
ACCTCAACGGGATCAACTTCCTCTTGCGTTCCCAGCTCAGTCTCGATGCCCCCACCTTTA
GCCGGATTGAGTATTCCTCCAGTGGCCACTCCCACATCACAAGAAGAATCAATTACTAAC
AGTGGACAACCTGTGGGTCTACTGGCTAGCGCTTTGAACGGAACGGCAAACGACGTCTTG
TCCAGTGATTCCATTCCTAATGATCCCATAACTCCGGGCTTGGCCACGGCATTGAGTGAA
ATGCTCCTGCACACCGGTGTGCCAAAGTTGATAGCTCGTCCACGCGGGGCTCTCCGATCG
GATGGTTCACAAATCTATCCCTCGCAAGCCGGACCGGTCTCGCAAAAATTGAAGGAGAAC
GCACACAAGATAACCGACTACTTCATCTCCGTCATCGAGGACACGCTTAGCGACATGGCT
ACAGGGGACCAAAGTGTCCTGCAAGCCCGGATTGCCGGCCTGTCGCTGGAGAACGAGCGT
CTGAAGCAGCACTATGACCGTCAAATAAACGACTTACATCGCACGAGCGAGCTAATGATT
TCCGAAATGCGAAAGACTTTGGAGCAAGAGCACAAACGCGTGATCTCCGAACTGCGACAG
CAGAACGCCATCGAGCTGATGCGGGCGGTCGAGGAGGCAAAGCGGAAGCAGTGGTGTGCG
AATTGCATGCGTGAGGCGCAGTTGTACTGCTGTTGGAACACATCCTACTGTGACTATCCC
TGCCAGCAGCTTCACTGGCCTGGACATTCTGCCACTTGTGGTCAGTCTGTGCCACCTACA
ATTCCAGTTCCACCTTCAGTTCCGACTCCGATTATAGAACCTGGACGTGCCAAAGCTAAA
GTGGCCACGCCAACGGCAACTCCGTCAATTACAAACCCAAGCCCCAGTTCGCAGATAATG
CGAACAGTAGCCGCCTGTCCATCTGCACCACCTAGTGCCAACGCTTCCAGTTCCAAAAAG
TGGCCGCCTATGATGACGTTAATGAATCAATCCAATCAGGAGGCCATGCTCAAGCTGCCC
GCCACTACATACCTTCGGCCAGTGGTCAGCACGACGATGACAGCTCCCGTGACTGCTCCG
CAACCAGCAAACAACAACAGCACGAACGTAATAATGGCACCCACACCACCGCCAGGAAAT
AATCTGGCCACTATAATCTCCGCACAGCGGAATCCCCCCAACTACAACGCCAAGCACCCC
AATCCAGCGATGCCCGTGCAACGCTTTAATATTCCTTTGCCCATCACCGTAAATTCGAAT
GCACCCTTTATGATGGCAGAGCAGCATCAGAAGCAAGTACCAAAAGCTACTGGTCGCTCA
GGTAAAAACAATAGCCGAATGCGCCAAACTTACAGCAACAATATCAACAACAGCAACCCG
CAGGGTATGCGCTGCAACAACAACCCGCAGGCTATCCGCCAGAATCAAATGAATCAACAG
GTATTCCAGCCA
Protein sequence predicted based on nucleotide sequence
MESTDSSDSSGDSLKSIPRPDFEALSAIGSPQPQLQSSSLDGHVEMEPVIMPSKRQKYIV SAPPGSANCSSNSSSSSPSNINVGSSLTMKLTKVQPQQHQAKSSTPNNKGKVAKEMVALQ RSHIESEVLSNFVTGVSKLKSRKSRYVPGNLATQAVGGELSRSLNTSSENENKSLKVALK RSSSIPAPILDSDDDWQDEDIAWGVTTCRPRGRSMAFEDGERLANLNAEDRNIVPAYSMA TRCRSRSKTLSLSNSWSNDDKGPRRSNLRSENEEFAKKHNAFLDRIIHDDRESELALNTS GEGESSLFSDASDTKTTTAKTPEEEAAERLALIYEEPPTPGWDPFCWKCRGCGKLMPCSK CLRSFHSYCVRPATTKFDSSWKCPECQVIEAAPKRLRRNGVSVDLLSQLLSFALDRMKHV RGAHKLRSPLEVFPLTYKKFFVNPVSFESLAQCIRNGAYQSTDEFLSEVKWIQHNALILD AGDAKVEQASKAVVKVCRQEANEIDTCPECYLNANSSDEWFVKVCRHPHLLLWAKLKGFP YWPAKAMGSSNSTLVNVRFFGKHDRAFVPVKDCFLYSAQNPNTQTSRRSARDLAECIREV EIHIDHIKRKIGAFNYAPYRTPYDPLEEQQQLEQMMPGVYAAIDRELEPANKTPLQFLIR KTADDKLSIVKKTKATESGNESDQSPSPTKKLSEVDVVSVTGSGCSDHSNVKSNNYEVIS RSGESLTDSRCKVLLKRKSLAAKIVAESVETSEAVAPKRKHSLSDASFTSESSEHKRKSK HARKQHDNQDNQIEEAEKTGQEPPKSPTISKQNENLRDEENVIENAANDTSSASPVSASV VSVVELVRRRQGVTITKIPREQQQTAEDTAAVPIPLPTAPPPKQNIPKGNEAANPKQSDV ERQQEQLIKKVIPFIEIKTEVMSEPDDEGIEEASPANQPQTDQVPLQQETITAQPESQMP AAAPAPQPKPVDNAPFEEVRIKEEILSEDEMETEQSIVSRRLKSVPPMPLPMPPPPPLPP REESPATADSVRFVGDTTIQRVSQKQGGKSTDTGGKRKGVQQVPIAGIPQAPSPTHSPLL STAPSPSASPKPTSTLAVSKPPPPPPPKGKSASHLQFRGKNDRIPTSPPATAPPATNTSS LLRSNMVVIPVEQGGSCNAHSPMTIPVPPLRAVSKNTLQNTSTGSTSSCVPSSVSMPPPL AGLSIPPVATPTSQEESITNSGQPVGLLASALNGTANDVLSSDSIPNDPITPGLATALSE MLLHTGVPKLIARPRGALRSDGSQIYPSQAGPVSQKLKENAHKITDYFISVIEDTLSDMA TGDQSVLQARIAGLSLENERLKQHYDRQINDLHRTSELMISEMRKTLEQEHKRVISELRQ QNAIELMRAVEEAKRKQWCANCMREAQLYCCWNTSYCDYPCQQLHWPGHSATCGQSVPPT IPVPPSVPTPIIEPGRAKAKVATPTATPSITNPSPSSQIMRTVAACPSAPPSANASSSKK WPPMMTLMNQSNQEAMLKLPATTYLRPVVSTTMTAPVTAPQPANNNSTNVIMAPTPPPGN NLATIISAQRNPPNYNAKHPNPAMPVQRFNIPLPITVNSNAPFMMAEQHQKQVPKATGRS GKNNSRMRQTYSNNINNSNPQGMRCNNNPQAIRQNQMNQQVFQP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
