Gene:
Gene Symbol:
CYFIP2
Gene Name:
cytoplasmic FMR1 interacting protein 2
Original Clone ID:
FLH226378.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HsCD00077490
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGACCACGCACGTCACCCTGGAAGATGCCCTGTCCAACGTGGACCTGCTTGAAGAGCTT
CCCCTCCCCGACCAGCAGCCATGCATCGAGCCTCCACCTTCCTCCATCATGTACCAGGCT
AACTTTGACACAAACTTTGAGGACAGGAATGCATTTGTCACGGGCATTGCAAGGTACATT
GAGCAGGCTACAGTCCACTCCAGCATGAATGAGATGCTGGAGGAAGGACATGAGTATGCG
GTCATGCTGTACACCTGGCGCAGCTGTTCCCGGGCCATTCCCCAGGTGAAATGCAACGAG
CAGCCCAACCGAGTAGAGATCTATGAGAAGACAGTAGAGGTGCTGGAGCCGGAGGTCACC
AAGCTCATGAAGTTCATGTATTTTCAGCGCAAGGCCATCGAGCGGTTCTGCAGCGAGGTG
AAGCGGCTGTGCCATGCCGAGCGCAGGAAGGACTTTGTCTCTGAGGCCTACCTCCTGACC
CTTGGCAAGTTCATCAACATGTTTGCTGTCCTGGATGAGCTAAAGAACATGAAGTGCAGC
GTCAAGAATGACCACTCTGCCTACAAGAGGGCAGCACAGTTCCTGCGGAAGATGGCAGAT
CCCCAGTCTATCCAGGAGTCGCAGAACCTTTCCATGTTCCTGGCCAACCACAACAGGATC
ACCCAGTGTCTCCACCAGCAACTTGAAGTGATCCCAGGCTATGAGGAGCTGCTGGCTGAC
ATTGTCAACATCTGTGTGGATTACTACGAGAACAAGATGTACCTGACTCCCAGTGAGAAA
CATATGCTCCTCAAGGTGATGGGCTTTGGCCTCTACCTAATGGATGGAAATGTCAGTAAC
ATTTACAAACTGGATGCCAAGAAGAGAATTAATCTTAGCAAAATTGATAAATTCTTTAAG
CAGCTGCAGGTGGTGCCCCTTTTCGGCGACATGCAGATAGAGCTGGCCAGATACATTAAG
ACCAGTGCTCACTATGAAGAGAACAAGTCCAAGTGGACGTGCACCCAGAGCAGCATCAGC
CCCCAGTACAATATCTGCGAGCAGATGGTTCAGATCCGGGATGACCACATCCGCTTCATC
TCCGAGCTCGCTCGCTACAGCAACAGTGAGGTGGTGACGGGCTCAGGGCTGGACAGCCAG
AAGTCAGACGAGGAGTATCGCGAGCTCTTCGACCTAGCCCTGCGGGGTCTGCAGCTTCTA
TCCAAGTGGAGCGCCCACGTCATGGAGGTGTACTCTTGGAAGCTGGTTCATCCCACAGAC
AAGTTCTGCAACAAGGACTGTCCTGGCACCGCGGAGGAATATGAGAGAGCCACACGCTAC
AATTACACCAGTGAGGAAAAATTTGCCTTCGTTGAGGTGATCGCCATGATCAAAGGCCTG
CAGGTGCTCATGGGCAGGATGGAGAGCGTCTTCAACCAGGCCATCAGGAACACCATCTAC
GCGGCATTGCAGGACTTCGCCCAGGTGACGCTGCGTGAGCCCCTGCGGCAGGCGGTACGG
AAGAAGAAGAATGTCCTCATCAGCGTCCTACAGGCAATTCGAAAGACCATCTGTGACTGG
GAGGGAGGGCGAGAGCCCCCTAATGACCCATGCTTGAGAGGGGAGAAGGACCCCAAAGGT
GGATTTGATATCAAGGTGCCCCGGCGTGCTGTGGGGCCATCCAGCACACAGCTGTACATG
GTGCGGACCATGCTTGAATCACTCATTGCAGACAAAAGCGGCTCCAAGAAGACCCTGAGG
AGCAGCCTGGATGGACCCATTGTCCTCGCCATAGAGGACTTTCACAAACAGTCCTTCTTC
TTCACACATCTGCTCAACATCAGTGAAGCCCTGCAGCAGTGTTGTGACCTCTCCCAGCTC
TGGTTCCGAGAATTCTTCCTGGAGTTAACCATGGGCCGACGAATCCAGTTCCCCATCGAG
ATGTCCATGCCCTGGATTCTAACGGACCATATCCTGGAAACCAAAGAACCTTCCATGATG
GAGTATGTCCTCTACCCTCTGGATCTGTACAACGACAGCGCCTACTATGCTCTGACCAAG
TTTAAAAAGCAGTTCCTGTACGATGAGATAGAAGCTGAGGTGAACCTGTGTTTTGATCAG
TTTGTCTACAAGCTGGCAGACCAGATCTTTGCTTACTACAAAGCCATGGCTGGCAGTGTC
CTGTTGGATAAACGTTTTCGAGCTGAGTGTAAGAATTATGGCGTCATCATTCCGTATCCA
CCGTCCAATCGCTATGAAACACTGCTGAAGCAGAGACACGTCCAGCTGTTGGGTAGATCA
ATTGACTTGAACAGACTCATTACCCAGCGCACCTCTGCCGCCATGTATAAATCCTTGGAC
CAAGCTATCAGCCGCTTTGAGAGTGAGGACCTGACCTCCATTGTGGAGCTGGAGTGGCTG
CTGGAGATTAACCGGCTCACGCATCGGCTGCTCTGTAAGCATATGACGCTGGACAGCTTC
GATGCCATGTTCCGAGAGGCCAATCACAATGTGTCCGCCCCCTATGGCCGTATCACCCTG
CATGTCTTCTGGGAACTGAACTTTGACTTTCTCCCCAACTACTGCTACAATGGGTCCACT
AACCGTTTTGTGCGGACTGCCATTCCTTTCACCCAAGAACCACAACGAGACAAACCTGCC
AACGTCCAGCCTTATTACCTCTATGGATCCAAGCCTCTCAACATTGCCTACAGCCACATC
TACAGCTCCTACAGGAATTTCGTGGGGCCACCTCATTTCAAGACTATCTGCAGACTCCTG
GGTTATCAGGGCATCGCTGTGGTCATGGAGGAACTGCTAAAGATTGTGAAGAGCTTGCTC
CAAGGAACCATTCTCCAGTATGTGAAAACACTGATAGAGGTGATGCCCAAGATATGCCGC
TTGCCCCGACATGAGTATGGCTCCCCAGGGATCCTGGAGTTCTTCCACCACCAGCTGAAG
GACATCATTGAGTACGCAGAGCTCAAAACAGACGTGTTCCAGAGCCTGAGGGAAGTGGGC
AATGCCATCCTCTTCTGCCTCCTCATAGAGCAAGCTCTGTCTCAGGAGGAGGTCTGCGAT
TTGCTCCATGCCGCACCCTTCCAAAACATCTTGCCTAGAGTCTACATCAAAGAGGGGGAG
CGCCTGGAGGTCCGGATGAAACGTCTGGAAGCCAAGTATGCCCCGCTCCACCTGGTCCCT
CTGATCGAGCGGCTGGGGACCCCTCAGCAAATCGCCATTGCTCGCGAGGGTGACCTCCTG
ACCAAGGAGCGGCTGTGCTGTGGCCTGTCCATGTTCGAGGTCATCCTGACCCGCATTCGG
AGCTACCTGCAGGACCCCATCTGGCGGGGCCCACCGCCCACCAATGGCGTCATGCACGTC
GATGAGTGTGTGGAGTTCCACCGGCTGTGGAGCGCCATGCAGTTCGTGTACTGCATCCCT
GTGGGAACCAACGAGTTCACAGCTGAGCAGTGTTTCGGCGATGGCTTGAACTGGGCTGGT
TGCTCCATCATTGTCCTGCTGGGCCAGCAGCGTCGCTTTGACCTGTTCGACTTCTGTTAC
CACCTGCTAAAAGTGCAGAGGCAGGACGGGAAGGATGAAATCATTAAGAATGTGCCCCTG
AAGAAGATGGCCGACCGGATCAGGAAGTATCAGATCTTGAACAATGAGGTTTTTGCCATC
CTGAACAAATACATGAAGTCCGTGGAGACAGACAGTTCCACTGTGGAGCATGTGCGCTGC
TTCCAGCCACCCATCCACCAGTCCTTGGCCACCACTTGC
Protein sequence predicted based on nucleotide sequence
MTTHVTLEDALSNVDLLEELPLPDQQPCIEPPPSSIMYQANFDTNFEDRNAFVTGIARYI EQATVHSSMNEMLEEGHEYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVT KLMKFMYFQRKAIERFCSEVKRLCHAERRKDFVSEAYLLTLGKFINMFAVLDELKNMKCS VKNDHSAYKRAAQFLRKMADPQSIQESQNLSMFLANHNRITQCLHQQLEVIPGYEELLAD IVNICVDYYENKMYLTPSEKHMLLKVMGFGLYLMDGNVSNIYKLDAKKRINLSKIDKFFK QLQVVPLFGDMQIELARYIKTSAHYEENKSKWTCTQSSISPQYNICEQMVQIRDDHIRFI SELARYSNSEVVTGSGLDSQKSDEEYRELFDLALRGLQLLSKWSAHVMEVYSWKLVHPTD KFCNKDCPGTAEEYERATRYNYTSEEKFAFVEVIAMIKGLQVLMGRMESVFNQAIRNTIY AALQDFAQVTLREPLRQAVRKKKNVLISVLQAIRKTICDWEGGREPPNDPCLRGEKDPKG GFDIKVPRRAVGPSSTQLYMVRTMLESLIADKSGSKKTLRSSLDGPIVLAIEDFHKQSFF FTHLLNISEALQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEPSMM EYVLYPLDLYNDSAYYALTKFKKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKAMAGSV LLDKRFRAECKNYGVIIPYPPSNRYETLLKQRHVQLLGRSIDLNRLITQRTSAAMYKSLD QAISRFESEDLTSIVELEWLLEINRLTHRLLCKHMTLDSFDAMFREANHNVSAPYGRITL HVFWELNFDFLPNYCYNGSTNRFVRTAIPFTQEPQRDKPANVQPYYLYGSKPLNIAYSHI YSSYRNFVGPPHFKTICRLLGYQGIAVVMEELLKIVKSLLQGTILQYVKTLIEVMPKICR LPRHEYGSPGILEFFHHQLKDIIEYAELKTDVFQSLREVGNAILFCLLIEQALSQEEVCD LLHAAPFQNILPRVYIKEGERLEVRMKRLEAKYAPLHLVPLIERLGTPQQIAIAREGDLL TKERLCCGLSMFEVILTRIRSYLQDPIWRGPPPTNGVMHVDECVEFHRLWSAMQFVYCIP VGTNEFTAEQCFGDGLNWAGCSIIVLLGQQRRFDLFDFCYHLLKVQRQDGKDEIIKNVPL KKMADRIRKYQILNNEVFAILNKYMKSVETDSSTVEHVRCFQPPIHQSLATTC
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
266
End on reference sequence
4027
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
